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pDUAL-HFG41

S. pombe 5 ter end His6-FLAG-GFP(S65C)-tagged vector with nmt1 (medium strength) promoter

Catalog number RDB06183
Resource name pDUAL-HFG41
Clone info. S. pombe 5 ter end His6-FLAG-GFP(S65C)-tagged vector with nmt1 (medium strength) promoter.
Comment Multicopy marker, ura4+; Integration marker, leu1+. Gateway incompatible.
Vector backbone pDUAL (Plasmid)
Selectable markers Amp^r
Reference of insert sequence AB364050.1 (DDBJ accession number)
Depositor|Developer Yoshida, Minoru | Matsuyama, Akihisa |
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External Database

            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
            Ordering Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
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            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
            提供案内 (日本国内) [open/close]

            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) の引用と、 寄託者への謝辞の表明を必要とする。 利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。 寄託者から要請がある場合、理研BRCは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
            GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします (Schedule AとSchedule Bを入手[link])。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB06183 pDUAL-HFG41 DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Sequence RDB06183z.seq

            Original reference

            original Matsuyama, A., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913.
            original Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Expression Vector Backbone of Schizosaccharomyces pombe (English text)

            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB14456_A6L7p1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: nmt1Fw
            Sequence file: RDB14456_A6L7a.seq check
            >06183_14456_A6L7_nmt1Fw_D01_04_ABI08.ab1
                1 CAAAAATTTA TTAAATCAGG AAAACGTAAC TCTCGGCTAC TGGATGGTTC AGTCACCCAA
               61 CGATTACTGG GGAGAGAAAA CAGGGCAAAA GCAAAGCTTA AAGGAATCCG ATTGTCATTC
              121 GGCAATGTGC AGCGAAACTA AAAACCGGAT AATGGACCTG TTAATCGAAA CATTGAAGAT
              181 AAAGGAAGAG GAATCCTGGC ATATCATCAA TTGAATAAGT TGAATTAATT ATTTCAATCT
              241 CATTCTCACT TTCTGACTTA TAGTCGCTTT GTTAAAGCTA GCGTCGACAG GCCTGGATCC
              301 TATGCATCAC CACCATCATC ATATGGATTA TAAGGATGAT GACGATAAAC CCATGGGTAA
              361 AGGAGAAGAA CTTTTCACTG GAGTTGTCCC AATTCTTGTT GAATTAGATG GTGATGTTAA
              421 TGGGCACAAA TTTTCTGTCA GTGGAGAGGG TGAAGGTGAT GCAACATACG GAAAACTTAC
              481 CCTTAAATTT ATTTGCACTA CTGGAAAACT ACCTGTTCCG TGGCCAACAC TTGTCACTAC
              541 TTTCTGTTAT GGTGTTCAAT GCTTTTCAAG ATACCCAGAT CACATGAAAC AGCATGACTT
              601 TTTCAAGAGT GCCATGCCCG AAGGCTATGT ACAGGAAAGA ACTATATTTT TCAAAGATGA
              661 CGGGAACTAC AAGACACGTG CTGAAGTCAA GTTTGAAGGT GATACCCTTG TTAATAGAAT
              721 CGAGTTAAAA GGTATTGATT TTAAAGAAGA TGGAAACATT CTTGGACACA AATTGGAATA
              781 CAACTATAAC TCACACAATG TATACATCAT GGCAGACAAA CAAAAGAATG GAATCAAAGT
              841 TAACTTCAAA ATTAGACACA ACATTGAAGA TGGAAGCGTT TCAACTAGCA GACCATTATC
              901 AACAAAATAC TCCAAATTGG CGATGGCCCT GTCCTTTTAC CAGACAAACC ATTACCCTGT
              961 CCACACAATC TGCCTTTTCG AAAGATCCCA CCGAAAAGAG AGACACAATG GTTCGTTCCT
             1021 GAGTTTGGTA CAGCTGGCTT GGAATTACAC ATTGGCATGG AATGAACTTA TACACGATAT
            1081 CATGAAATCT CGAACCTCTA GAGGAATCG
            //
            Primer: ADH_rev
            Sequence file: RDB14456_A6L7b.seq check
            >06183_14456_A6L7_ADH_rev_E01_05_ABI08.ab1
                1 GGAAGGAGTT CCTCAGGATA GGAATTTTCG TTTTAAAACC TAAGAGTCAC TTTAAAATTT
               61 GTATACACTT ATTTTTTTTA TAACTTATTT AATAATAAAA ATCATAAATC ATAAGAAATT
              121 CGCTTATTTA GAAGTGGCGC GCCGGATCCT CTAGAGTCGA GATCTCATGA TATCTTTGTA
              181 TAGTTCATCC ATGCCATGTG TAATCCCAGC AGCTGTTACA AACTCAAGAA GGACCATGTG
              241 GTCTCTCTTT TCGTTGGGAT CTTTCGAAAG GGCAGATTGT GTGGACAGGT AATGGTTGTC
              301 TGGTAAAAGG ACAGGGCCAT CGCCAATTGG AGTATTTTGT TGATAATGGT CTGCTAGTTG
              361 AACGCTTCCA TCTTCAATGT TGTGTCTAAT TTTGAAGTTA ACTTTGATTC CATTCTTTTG
              421 TTTGTCTGCC ATGATGTATA CATTGTGTGA GTTATAGTTG TATTCCAATT TGTGTCCAAG
              481 AATGTTTCCA TCTTCTTTAA AATCAATACC TTTTAACTCG ATTCTATTAA CAAGGGTATC
              541 ACCTTCAAAC TTGACTTCAG CACGTGTCTT GTAGTTCCCG TCATCTTTGA AAAATATAGT
              601 TCTTTCCTGT ACATAGCCTT CGGGCATGGC ACTCTTGAAA AAGTCATGCT GTTTCATGTG
              661 ATCTGGGTAT CTTGAAAAGC ATTGAACACC ATAACAGAAA GTAGTGACAA GTGTTGGCCA
              721 CGGAACAGGT AGTTTTCCAG TAGTGCAAAT AAATTTAAGG GTAAGTTTTC CGTATGTTGC
              781 ATCACCTTCA CCCTCTCCAC TGACAGAAAA TTTGTGCCCA TTAACATCAC CATCTAATTC
              841 AACAAGAAAT TGGGACAACT CCAGTGAAAA GTTCTTCTCC TTTACCCATG GGTTTATCGT
              901 CATCATCCTT ATAATCCATA TGATGATGGT GGTGATGCAT AGGATCCAGG CCCTGTCGAC
              961 GCTAGCTTTA ACAAAGCGAC TATAAGTCAG AAAGTGAGAT GAGATTGAAA TAGTAAATCA
             1021 CTTATTCAAA TTGAATGAAT ATGGCCAGGA TTTCCTTCGT GCGTAATCCT CAATGTTTCG
            1081 AATTAAACCA GGTCCCCAGA T
            //
            Primer: ADH-Forward
            Sequence file: RDB14456_A6L7c.seq check
            >06183_14456_A6L7_ADH-Forward_F01_06_ABI08.ab1
                1 TATCCCCTTT TTTTGACACA CCTCTACCGG CAGATCCGCT AGGGATAACA GGGTAATATA
               61 CTAGTAAGAT GATTCTTTTA TCGTCGATAA CGATGAGGAT GTAGACGATT ATGATGAGAG
              121 TGATTAGCTT CTAAAATTTG GTGTTTTCAA ACGCTATTTA CAGTACAATC TGAAAAAATT
              181 GGATGAACAT TGTAAATGGT AGGATACGAG TTCCAATTAA TGTATTTTTT AAGTATTACA
              241 CTTAGCTTAT TAATTTTATA TATACATTCT ACCTCTGTGG GTTTAAAGAC AACTCGTACA
              301 CATACTCAAA TTATTTATGA ACATTATAAT GCATATTCGT ACTGTCTATT AAACATTTTT
              361 TTGACATTAA AAATTTCGTT TACTAACGTA GAAAGCGCTG ATAATTGAGG CTACTATTTT
              421 AATATCTAGA AACAAAGATT CATTAATTAA TTAAGTAAGC TCCAGGATAC TTGTATATTT
              481 CGTTAAAAAA GCGCAATTTC AACAATTCCT ATGAACATCC ATGATTTCAT CACAAAATCA
              541 AACATATCAG GATTTAACGT GAATTATTTG CTTTCTTGTG ATTTTCATCT GATTTCTGGT
              601 CATTTACGTT ACTGTAAACA AGCTTTTTAA AAAAAGGATT TTTTACGCAA TATTATCGAT
              661 ATCCCAATCT GTAGTATAAA AAGTGCAACA GTGTTGAGTT TCCCGAAACC AGGCATTTTT
              721 GCGGTAGGGT CTGAAACTTT CACGAAACCA TTTCACAACA AAACTTCTTC AACAACATGT
              781 GTGCAAAGAA AATCGTCGTC TTACCAGGAG ACCATATTGG CCCTGAAATT GTTGCTTCTG
              841 CCTTGGAGGT TTTGAAAGTC GTTGAGAAGA AGCGACCTGA GTTAAAACTC GAGTTTGAAG
              901 AACACAAGAT TGGAGGTGCC TCTATTGATG CCTATGGAAC CCCTTTGACT GACGAGACTG
              961 TGGAAGGCTT GTTTGGGAAG GCTGACGGGT GTCTTTTGGG TTGCGTTTGG TGGGTCTGGA
             1021 ATGATACCCC AATGTCGGTC TGAGCCAAGT TTATTGAGCT TCGTAGAGTA TGGGGAGTAG
            1081 GGTCAAACCC TTTG
            //
            Primer: ars_R1
            Sequence file: RDB14456_A6L7d.seq check
            >06183_14456_A6L7_ars_R1_G01_07_ABI08.ab1
                1 TGGGTTTCGG TTTAATACTA AATTACTTAA AGTACCTTTA ACTAAACAAA ATGCCTATAT
               61 ATATATTAAT TTACAATGAG TGTCAGATAA GTCACTATGT CCGAGTGGTT AAGGAGTTAG
              121 ACTCGAATTC GCAAGCTTAG CTACAAATCC CACTGGCTAT ATGTATGCAT TTGTGTTAAA
              181 AAAGTTTGTA TAGATTATTT AATCTACTCA GCATTCTTTC TCTAAATAGG AATTTGTTAC
              241 TTAATGGAGA AAAAAATGTT TCGATTTACC TAGTGTATTT GTTTGTATAC TCACGTTTAA
              301 TTTCAAACAT CCATTCTATC TTGTGTAATT TTTGGCATGG TGAAAAAGAT AATCAGCCTT
              361 ATAATCTTTA CAAAAGTAAG AAATTCTGTA AATAAGCCTT AATGCCCTTG CTTTAAATTA
              421 AAATGGTTCT TTTTCATGAT AATGTTTGCA CTTTGTGAAT ATATTTTAGA TAGTTCTGTG
              481 AGGTATAATT AAGATGTTTT AGAGACTTAT ACAATTTTGT CTTTATAAAT TCTTAATTGA
              541 TTTTACCATC CCAGTTTAAC TATGCTTCGT CGGCATCTCT GCACATGTCG TGTTTTCTTA
              601 CCGTATTGTC CTACCAAGAA CCTCTTTTTT GCTTGGATCG AAATTAAAGG TTTAAAAGCA
              661 AAGTTATGGA TGCTAGAGTA TTTCAAAGCT ATTCAGCTAG AGCTGAGGGG ATGAAAAAAT
              721 CCCATTGCCA AGGAATTGTT GGCTTTGATG GAAGAAAAGC AAAGCAACTT GTCAGTCGCG
              781 GTCGATTTGA CGAAGAAATC CGAAATCTTA GAATTGGGTA GATAAAATTG GGACCCTATG
              841 TCTGTGTTAT CAAGACACAT ATTGACGTTG TCGAGGATTT CGACCAAGGA TATGGGTAGA
              901 AAAAACTGGT GGCCTTAGGT AAAAAAGCAT CGTTTTCTTA TCCTTTGAAG GATCGCAAAT
              961 TCGCCAGAAC AATTGGAAAA ATACCGGTCC AAGCTTACAA TAATGGCATC TGGGTGGTGT
             1021 ACAAAAATTT GCCTTCTTGG GGCTCAATAA TCAAACAAAT TGCCAATAAC AGTGGCCAAG
            1081 CCGAGGGTAT AATACCGAGG CCTTCAAAAG AAAAGCCTTG TGGT
            //
            Primer: f1ori_R3
            Sequence file: RDB14456_A6L7e.seq check
            >06183_14456_A6L7_f1ori_R3_E01_13_ABI24.ab1
                1 GGCAGGGGAT GCTTGAGTAT GCGGTGTGAA TACCGCACAG ATGCGTAAGG AGAAAATACC
               61 GCATCAGGCG CCATTCGCCA TTCAGGCTGC GCAACTGTTG GGAAGGGCGA TCGGTGCGGG
              121 CCTCTTCGCT ATTACGCCAG CTGGCGAAAG GGGGATGTGC TGCAAGGCGA TTAAGTTGGG
              181 TAACGCCAGG GTTTTCCCAG TCACGACGTT GTAAAACGAC GGCCAGTGAA TTCGAGCTCG
              241 GGAGCGCTAC CGTGAATGGG CTCAACCAAA CAATGAACCT TTTCTTCTGA TTTTCCTACC
              301 ACACCGGAAA GGGAGGCAGA AGGCAAAAGG CCCAAGCTAC CAGGAATGAC AGAAGCCTCA
              361 TCTGAAATAA TGTCACCAAA CAAGTTGTCA GTCAAAACAA CACCGTTAAG TGTACGAGGG
              421 CTCTTGACCA AAAGCATGGC TGCGGAGTCA ATGAGCTGGT TTTTTAAGGT AAGGTGAGGA
              481 TATTCCTCCT TAAAAATCTT AGCTACAGTC TTGCGCCAAA GACGAGAAGT TGCCAAAACA
              541 TTAGCTTTGT CGAGTAATGT GACGGGAGCA GGAGGGTTGG AAGTTTCAGC TAAGCGGCCG
              601 CGGGCCCAAC CTTCCAATTC ATTAAATCGA GTCCTCCATC AGATGATTTA CAAGGTTTTG
              661 CATAGAATCC CTAAGTAAGA GAAGTTGTTA GTGATTTCTT CAAGTTTACT AAAATTTACA
              721 TACAAATGGA TGATCTCTTC TCCATTGTTT CCTTTCTTCT TGCAAACGCG TTTTACAAAG
              781 AGATGACATG CTATTTTCTG ATTATTTTTT TCTATAGTTT TCTATTTTGT ACGTCCTTGG
              841 GAGTCCGAAA TGTAAAATCG GGGTATGGTT AGTAGCGGCA GCGTTCATTA TGGTATATAA
              901 AAGATAAAAA GTAGGGCAAG CGAGACAGAA TGGGATACAA GGGCATCGTC TATAATTATA
              961 GCTAAAATTG TATTTTAATT TGTATTTTTT GTAATTTTTA TTTCTCATGT TTACTTAAAT
             1021 GAATGCGATT AGAGAAAAAC TTTCACGAAT TGTCAAATAG CTCAGCAAAT ATACATTTTA
             1081 GAAGCGAATC AGCACTTTAT TCTTCCGGTT GGTAGATTCG TTCGTCATAA ATTCGACATG
            1141 TAACTGCAAT TTGGAAGTAC CCTCGGGATG
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Matsuyama, A., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913.
            original Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162.
            reference Kitamura, K., Inhibition of the Arg/N-end rule pathway-mediated proteolysis by dipeptide-mimetic molecules. Amino Acids 48 (1): 235-243 ((2016). PMID 26334349.
            reference Kitamura, K., The type-2 N-end rule peptide recognition activity of Ubr11 ubiquitin ligase is required for the expression of peptide transporters. FEBS Lett. 587 (2): 214-219 (2013). PMID 23219921.

            2022.07.13

            GNP_filter3_RDBDEP_html_220713.pl