Please review the QC test results indicated by icon below as well as clone information before placing your order.

pDUAL-HFG41

S. pombe 5 ter end His6-FLAG-GFP(S65C)-tagged vector with nmt1 (medium strength) promoter

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR

In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.

Ordering

Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word] This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).

提供案内 (日本国内) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献 (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) の引用と、　寄託者への謝辞の表明を必要とする。　利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。　寄託者から要請がある場合、理研ＢＲＣは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word] GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします （Schedule AとSchedule Bを入手[link])。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB14456_A6L7p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: nmt1Fw Sequence file: RDB14456_A6L7a.seq
>06183_14456_A6L7_nmt1Fw_D01_04_ABI08.ab1     1 CAAAAATTTA TTAAATCAGG AAAACGTAAC TCTCGGCTAC TGGATGGTTC AGTCACCCAA    61 CGATTACTGG GGAGAGAAAA CAGGGCAAAA GCAAAGCTTA AAGGAATCCG ATTGTCATTC   121 GGCAATGTGC AGCGAAACTA AAAACCGGAT AATGGACCTG TTAATCGAAA CATTGAAGAT   181 AAAGGAAGAG GAATCCTGGC ATATCATCAA TTGAATAAGT TGAATTAATT ATTTCAATCT   241 CATTCTCACT TTCTGACTTA TAGTCGCTTT GTTAAAGCTA GCGTCGACAG GCCTGGATCC   301 TATGCATCAC CACCATCATC ATATGGATTA TAAGGATGAT GACGATAAAC CCATGGGTAA   361 AGGAGAAGAA CTTTTCACTG GAGTTGTCCC AATTCTTGTT GAATTAGATG GTGATGTTAA   421 TGGGCACAAA TTTTCTGTCA GTGGAGAGGG TGAAGGTGAT GCAACATACG GAAAACTTAC   481 CCTTAAATTT ATTTGCACTA CTGGAAAACT ACCTGTTCCG TGGCCAACAC TTGTCACTAC   541 TTTCTGTTAT GGTGTTCAAT GCTTTTCAAG ATACCCAGAT CACATGAAAC AGCATGACTT   601 TTTCAAGAGT GCCATGCCCG AAGGCTATGT ACAGGAAAGA ACTATATTTT TCAAAGATGA   661 CGGGAACTAC AAGACACGTG CTGAAGTCAA GTTTGAAGGT GATACCCTTG TTAATAGAAT   721 CGAGTTAAAA GGTATTGATT TTAAAGAAGA TGGAAACATT CTTGGACACA AATTGGAATA   781 CAACTATAAC TCACACAATG TATACATCAT GGCAGACAAA CAAAAGAATG GAATCAAAGT   841 TAACTTCAAA ATTAGACACA ACATTGAAGA TGGAAGCGTT TCAACTAGCA GACCATTATC   901 AACAAAATAC TCCAAATTGG CGATGGCCCT GTCCTTTTAC CAGACAAACC ATTACCCTGT   961 CCACACAATC TGCCTTTTCG AAAGATCCCA CCGAAAAGAG AGACACAATG GTTCGTTCCT  1021 GAGTTTGGTA CAGCTGGCTT GGAATTACAC ATTGGCATGG AATGAACTTA TACACGATAT 1081 CATGAAATCT CGAACCTCTA GAGGAATCG //
Primer: ADH_rev Sequence file: RDB14456_A6L7b.seq
>06183_14456_A6L7_ADH_rev_E01_05_ABI08.ab1     1 GGAAGGAGTT CCTCAGGATA GGAATTTTCG TTTTAAAACC TAAGAGTCAC TTTAAAATTT    61 GTATACACTT ATTTTTTTTA TAACTTATTT AATAATAAAA ATCATAAATC ATAAGAAATT   121 CGCTTATTTA GAAGTGGCGC GCCGGATCCT CTAGAGTCGA GATCTCATGA TATCTTTGTA   181 TAGTTCATCC ATGCCATGTG TAATCCCAGC AGCTGTTACA AACTCAAGAA GGACCATGTG   241 GTCTCTCTTT TCGTTGGGAT CTTTCGAAAG GGCAGATTGT GTGGACAGGT AATGGTTGTC   301 TGGTAAAAGG ACAGGGCCAT CGCCAATTGG AGTATTTTGT TGATAATGGT CTGCTAGTTG   361 AACGCTTCCA TCTTCAATGT TGTGTCTAAT TTTGAAGTTA ACTTTGATTC CATTCTTTTG   421 TTTGTCTGCC ATGATGTATA CATTGTGTGA GTTATAGTTG TATTCCAATT TGTGTCCAAG   481 AATGTTTCCA TCTTCTTTAA AATCAATACC TTTTAACTCG ATTCTATTAA CAAGGGTATC   541 ACCTTCAAAC TTGACTTCAG CACGTGTCTT GTAGTTCCCG TCATCTTTGA AAAATATAGT   601 TCTTTCCTGT ACATAGCCTT CGGGCATGGC ACTCTTGAAA AAGTCATGCT GTTTCATGTG   661 ATCTGGGTAT CTTGAAAAGC ATTGAACACC ATAACAGAAA GTAGTGACAA GTGTTGGCCA   721 CGGAACAGGT AGTTTTCCAG TAGTGCAAAT AAATTTAAGG GTAAGTTTTC CGTATGTTGC   781 ATCACCTTCA CCCTCTCCAC TGACAGAAAA TTTGTGCCCA TTAACATCAC CATCTAATTC   841 AACAAGAAAT TGGGACAACT CCAGTGAAAA GTTCTTCTCC TTTACCCATG GGTTTATCGT   901 CATCATCCTT ATAATCCATA TGATGATGGT GGTGATGCAT AGGATCCAGG CCCTGTCGAC   961 GCTAGCTTTA ACAAAGCGAC TATAAGTCAG AAAGTGAGAT GAGATTGAAA TAGTAAATCA  1021 CTTATTCAAA TTGAATGAAT ATGGCCAGGA TTTCCTTCGT GCGTAATCCT CAATGTTTCG 1081 AATTAAACCA GGTCCCCAGA T //
Primer: ADH-Forward Sequence file: RDB14456_A6L7c.seq
>06183_14456_A6L7_ADH-Forward_F01_06_ABI08.ab1     1 TATCCCCTTT TTTTGACACA CCTCTACCGG CAGATCCGCT AGGGATAACA GGGTAATATA    61 CTAGTAAGAT GATTCTTTTA TCGTCGATAA CGATGAGGAT GTAGACGATT ATGATGAGAG   121 TGATTAGCTT CTAAAATTTG GTGTTTTCAA ACGCTATTTA CAGTACAATC TGAAAAAATT   181 GGATGAACAT TGTAAATGGT AGGATACGAG TTCCAATTAA TGTATTTTTT AAGTATTACA   241 CTTAGCTTAT TAATTTTATA TATACATTCT ACCTCTGTGG GTTTAAAGAC AACTCGTACA   301 CATACTCAAA TTATTTATGA ACATTATAAT GCATATTCGT ACTGTCTATT AAACATTTTT   361 TTGACATTAA AAATTTCGTT TACTAACGTA GAAAGCGCTG ATAATTGAGG CTACTATTTT   421 AATATCTAGA AACAAAGATT CATTAATTAA TTAAGTAAGC TCCAGGATAC TTGTATATTT   481 CGTTAAAAAA GCGCAATTTC AACAATTCCT ATGAACATCC ATGATTTCAT CACAAAATCA   541 AACATATCAG GATTTAACGT GAATTATTTG CTTTCTTGTG ATTTTCATCT GATTTCTGGT   601 CATTTACGTT ACTGTAAACA AGCTTTTTAA AAAAAGGATT TTTTACGCAA TATTATCGAT   661 ATCCCAATCT GTAGTATAAA AAGTGCAACA GTGTTGAGTT TCCCGAAACC AGGCATTTTT   721 GCGGTAGGGT CTGAAACTTT CACGAAACCA TTTCACAACA AAACTTCTTC AACAACATGT   781 GTGCAAAGAA AATCGTCGTC TTACCAGGAG ACCATATTGG CCCTGAAATT GTTGCTTCTG   841 CCTTGGAGGT TTTGAAAGTC GTTGAGAAGA AGCGACCTGA GTTAAAACTC GAGTTTGAAG   901 AACACAAGAT TGGAGGTGCC TCTATTGATG CCTATGGAAC CCCTTTGACT GACGAGACTG   961 TGGAAGGCTT GTTTGGGAAG GCTGACGGGT GTCTTTTGGG TTGCGTTTGG TGGGTCTGGA  1021 ATGATACCCC AATGTCGGTC TGAGCCAAGT TTATTGAGCT TCGTAGAGTA TGGGGAGTAG 1081 GGTCAAACCC TTTG //
Primer: ars_R1 Sequence file: RDB14456_A6L7d.seq
>06183_14456_A6L7_ars_R1_G01_07_ABI08.ab1     1 TGGGTTTCGG TTTAATACTA AATTACTTAA AGTACCTTTA ACTAAACAAA ATGCCTATAT    61 ATATATTAAT TTACAATGAG TGTCAGATAA GTCACTATGT CCGAGTGGTT AAGGAGTTAG   121 ACTCGAATTC GCAAGCTTAG CTACAAATCC CACTGGCTAT ATGTATGCAT TTGTGTTAAA   181 AAAGTTTGTA TAGATTATTT AATCTACTCA GCATTCTTTC TCTAAATAGG AATTTGTTAC   241 TTAATGGAGA AAAAAATGTT TCGATTTACC TAGTGTATTT GTTTGTATAC TCACGTTTAA   301 TTTCAAACAT CCATTCTATC TTGTGTAATT TTTGGCATGG TGAAAAAGAT AATCAGCCTT   361 ATAATCTTTA CAAAAGTAAG AAATTCTGTA AATAAGCCTT AATGCCCTTG CTTTAAATTA   421 AAATGGTTCT TTTTCATGAT AATGTTTGCA CTTTGTGAAT ATATTTTAGA TAGTTCTGTG   481 AGGTATAATT AAGATGTTTT AGAGACTTAT ACAATTTTGT CTTTATAAAT TCTTAATTGA   541 TTTTACCATC CCAGTTTAAC TATGCTTCGT CGGCATCTCT GCACATGTCG TGTTTTCTTA   601 CCGTATTGTC CTACCAAGAA CCTCTTTTTT GCTTGGATCG AAATTAAAGG TTTAAAAGCA   661 AAGTTATGGA TGCTAGAGTA TTTCAAAGCT ATTCAGCTAG AGCTGAGGGG ATGAAAAAAT   721 CCCATTGCCA AGGAATTGTT GGCTTTGATG GAAGAAAAGC AAAGCAACTT GTCAGTCGCG   781 GTCGATTTGA CGAAGAAATC CGAAATCTTA GAATTGGGTA GATAAAATTG GGACCCTATG   841 TCTGTGTTAT CAAGACACAT ATTGACGTTG TCGAGGATTT CGACCAAGGA TATGGGTAGA   901 AAAAACTGGT GGCCTTAGGT AAAAAAGCAT CGTTTTCTTA TCCTTTGAAG GATCGCAAAT   961 TCGCCAGAAC AATTGGAAAA ATACCGGTCC AAGCTTACAA TAATGGCATC TGGGTGGTGT  1021 ACAAAAATTT GCCTTCTTGG GGCTCAATAA TCAAACAAAT TGCCAATAAC AGTGGCCAAG 1081 CCGAGGGTAT AATACCGAGG CCTTCAAAAG AAAAGCCTTG TGGT //
Primer: f1ori_R3 Sequence file: RDB14456_A6L7e.seq
>06183_14456_A6L7_f1ori_R3_E01_13_ABI24.ab1     1 GGCAGGGGAT GCTTGAGTAT GCGGTGTGAA TACCGCACAG ATGCGTAAGG AGAAAATACC    61 GCATCAGGCG CCATTCGCCA TTCAGGCTGC GCAACTGTTG GGAAGGGCGA TCGGTGCGGG   121 CCTCTTCGCT ATTACGCCAG CTGGCGAAAG GGGGATGTGC TGCAAGGCGA TTAAGTTGGG   181 TAACGCCAGG GTTTTCCCAG TCACGACGTT GTAAAACGAC GGCCAGTGAA TTCGAGCTCG   241 GGAGCGCTAC CGTGAATGGG CTCAACCAAA CAATGAACCT TTTCTTCTGA TTTTCCTACC   301 ACACCGGAAA GGGAGGCAGA AGGCAAAAGG CCCAAGCTAC CAGGAATGAC AGAAGCCTCA   361 TCTGAAATAA TGTCACCAAA CAAGTTGTCA GTCAAAACAA CACCGTTAAG TGTACGAGGG   421 CTCTTGACCA AAAGCATGGC TGCGGAGTCA ATGAGCTGGT TTTTTAAGGT AAGGTGAGGA   481 TATTCCTCCT TAAAAATCTT AGCTACAGTC TTGCGCCAAA GACGAGAAGT TGCCAAAACA   541 TTAGCTTTGT CGAGTAATGT GACGGGAGCA GGAGGGTTGG AAGTTTCAGC TAAGCGGCCG   601 CGGGCCCAAC CTTCCAATTC ATTAAATCGA GTCCTCCATC AGATGATTTA CAAGGTTTTG   661 CATAGAATCC CTAAGTAAGA GAAGTTGTTA GTGATTTCTT CAAGTTTACT AAAATTTACA   721 TACAAATGGA TGATCTCTTC TCCATTGTTT CCTTTCTTCT TGCAAACGCG TTTTACAAAG   781 AGATGACATG CTATTTTCTG ATTATTTTTT TCTATAGTTT TCTATTTTGT ACGTCCTTGG   841 GAGTCCGAAA TGTAAAATCG GGGTATGGTT AGTAGCGGCA GCGTTCATTA TGGTATATAA   901 AAGATAAAAA GTAGGGCAAG CGAGACAGAA TGGGATACAA GGGCATCGTC TATAATTATA   961 GCTAAAATTG TATTTTAATT TGTATTTTTT GTAATTTTTA TTTCTCATGT TTACTTAAAT  1021 GAATGCGATT AGAGAAAAAC TTTCACGAAT TGTCAAATAG CTCAGCAAAT ATACATTTTA  1081 GAAGCGAATC AGCACTTTAT TCTTCCGGTT GGTAGATTCG TTCGTCATAA ATTCGACATG 1141 TAACTGCAAT TTGGAAGTAC CCTCGGGATG //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.05.18

GNP_filter3_RDBDEP_html_220516.pl