Sequencing and PCR primers for primer information.
S. pombe 5 ter end His6-FLAG-GFP(S65C)-tagged vector with nmt1 (medium strength) promoter
| Catalog number | RDB06183 |
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| Resource name | pDUAL-HFG41 |
| Clone info. | S. pombe 5 ter end His6-FLAG-GFP(S65C)-tagged vector with nmt1 (medium strength) promoter. Multicopy marker, ura4+; Integration marker, leu1+. Gateway incompatible. |
| Vector backbone | pDUAL (Plasmid) |
| Selectable markers | Amp^r |
| Reference of nucleotide sequence | AB364050.1 (DDBJ accession number) |
| Depositor | Yoshida, Minoru | Matsuyama, Akihisa | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Terms and conditions set forth by the DEPOSITOR | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation. |
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| Ordering | Please visit Information of Request for Distribution. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word] Schedule A (GFP-A) [Link] |
| Remarks | ((Additional form)) GFP-A Please use Schedule A (GFP-A) additionally. |
[open/close]| Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
|---|---|---|---|
| RDB06183 | pDUAL-HFG41 | DNA solution |
Electronic file
| Electronic file | Sequence RDB06183z.seq from Depositor |
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Featured content
| Featured content | Expression Vector Backbone of Schizosaccharomyces pombe (English text) |
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References
User reports and related articles (go to bottom)
RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.
Data are summerized on test sheet below.
| Test sheet | RDB14456_A6L7p1.pdf |
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Nucleotide sequence of a portion of this resource (if available).
| Primer: nmt1Fw | RDB14456_A6L7a.seq |
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>06183_14456_A6L7_nmt1Fw_D01_04_ABI08.ab1 1 CAAAAATTTA TTAAATCAGG AAAACGTAAC TCTCGGCTAC TGGATGGTTC AGTCACCCAA 61 CGATTACTGG GGAGAGAAAA CAGGGCAAAA GCAAAGCTTA AAGGAATCCG ATTGTCATTC 121 GGCAATGTGC AGCGAAACTA AAAACCGGAT AATGGACCTG TTAATCGAAA CATTGAAGAT 181 AAAGGAAGAG GAATCCTGGC ATATCATCAA TTGAATAAGT TGAATTAATT ATTTCAATCT 241 CATTCTCACT TTCTGACTTA TAGTCGCTTT GTTAAAGCTA GCGTCGACAG GCCTGGATCC 301 TATGCATCAC CACCATCATC ATATGGATTA TAAGGATGAT GACGATAAAC CCATGGGTAA 361 AGGAGAAGAA CTTTTCACTG GAGTTGTCCC AATTCTTGTT GAATTAGATG GTGATGTTAA 421 TGGGCACAAA TTTTCTGTCA GTGGAGAGGG TGAAGGTGAT GCAACATACG GAAAACTTAC 481 CCTTAAATTT ATTTGCACTA CTGGAAAACT ACCTGTTCCG TGGCCAACAC TTGTCACTAC 541 TTTCTGTTAT GGTGTTCAAT GCTTTTCAAG ATACCCAGAT CACATGAAAC AGCATGACTT 601 TTTCAAGAGT GCCATGCCCG AAGGCTATGT ACAGGAAAGA ACTATATTTT TCAAAGATGA 661 CGGGAACTAC AAGACACGTG CTGAAGTCAA GTTTGAAGGT GATACCCTTG TTAATAGAAT 721 CGAGTTAAAA GGTATTGATT TTAAAGAAGA TGGAAACATT CTTGGACACA AATTGGAATA 781 CAACTATAAC TCACACAATG TATACATCAT GGCAGACAAA CAAAAGAATG GAATCAAAGT 841 TAACTTCAAA ATTAGACACA ACATTGAAGA TGGAAGCGTT TCAACTAGCA GACCATTATC 901 AACAAAATAC TCCAAATTGG CGATGGCCCT GTCCTTTTAC CAGACAAACC ATTACCCTGT 961 CCACACAATC TGCCTTTTCG AAAGATCCCA CCGAAAAGAG AGACACAATG GTTCGTTCCT 1021 GAGTTTGGTA CAGCTGGCTT GGAATTACAC ATTGGCATGG AATGAACTTA TACACGATAT 1081 CATGAAATCT CGAACCTCTA GAGGAATCG // |
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| Primer: ADH_rev | RDB14456_A6L7b.seq |
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>06183_14456_A6L7_ADH_rev_E01_05_ABI08.ab1 1 GGAAGGAGTT CCTCAGGATA GGAATTTTCG TTTTAAAACC TAAGAGTCAC TTTAAAATTT 61 GTATACACTT ATTTTTTTTA TAACTTATTT AATAATAAAA ATCATAAATC ATAAGAAATT 121 CGCTTATTTA GAAGTGGCGC GCCGGATCCT CTAGAGTCGA GATCTCATGA TATCTTTGTA 181 TAGTTCATCC ATGCCATGTG TAATCCCAGC AGCTGTTACA AACTCAAGAA GGACCATGTG 241 GTCTCTCTTT TCGTTGGGAT CTTTCGAAAG GGCAGATTGT GTGGACAGGT AATGGTTGTC 301 TGGTAAAAGG ACAGGGCCAT CGCCAATTGG AGTATTTTGT TGATAATGGT CTGCTAGTTG 361 AACGCTTCCA TCTTCAATGT TGTGTCTAAT TTTGAAGTTA ACTTTGATTC CATTCTTTTG 421 TTTGTCTGCC ATGATGTATA CATTGTGTGA GTTATAGTTG TATTCCAATT TGTGTCCAAG 481 AATGTTTCCA TCTTCTTTAA AATCAATACC TTTTAACTCG ATTCTATTAA CAAGGGTATC 541 ACCTTCAAAC TTGACTTCAG CACGTGTCTT GTAGTTCCCG TCATCTTTGA AAAATATAGT 601 TCTTTCCTGT ACATAGCCTT CGGGCATGGC ACTCTTGAAA AAGTCATGCT GTTTCATGTG 661 ATCTGGGTAT CTTGAAAAGC ATTGAACACC ATAACAGAAA GTAGTGACAA GTGTTGGCCA 721 CGGAACAGGT AGTTTTCCAG TAGTGCAAAT AAATTTAAGG GTAAGTTTTC CGTATGTTGC 781 ATCACCTTCA CCCTCTCCAC TGACAGAAAA TTTGTGCCCA TTAACATCAC CATCTAATTC 841 AACAAGAAAT TGGGACAACT CCAGTGAAAA GTTCTTCTCC TTTACCCATG GGTTTATCGT 901 CATCATCCTT ATAATCCATA TGATGATGGT GGTGATGCAT AGGATCCAGG CCCTGTCGAC 961 GCTAGCTTTA ACAAAGCGAC TATAAGTCAG AAAGTGAGAT GAGATTGAAA TAGTAAATCA 1021 CTTATTCAAA TTGAATGAAT ATGGCCAGGA TTTCCTTCGT GCGTAATCCT CAATGTTTCG 1081 AATTAAACCA GGTCCCCAGA T // |
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| Primer: ADH-Forward | RDB14456_A6L7c.seq |
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>06183_14456_A6L7_ADH-Forward_F01_06_ABI08.ab1 1 TATCCCCTTT TTTTGACACA CCTCTACCGG CAGATCCGCT AGGGATAACA GGGTAATATA 61 CTAGTAAGAT GATTCTTTTA TCGTCGATAA CGATGAGGAT GTAGACGATT ATGATGAGAG 121 TGATTAGCTT CTAAAATTTG GTGTTTTCAA ACGCTATTTA CAGTACAATC TGAAAAAATT 181 GGATGAACAT TGTAAATGGT AGGATACGAG TTCCAATTAA TGTATTTTTT AAGTATTACA 241 CTTAGCTTAT TAATTTTATA TATACATTCT ACCTCTGTGG GTTTAAAGAC AACTCGTACA 301 CATACTCAAA TTATTTATGA ACATTATAAT GCATATTCGT ACTGTCTATT AAACATTTTT 361 TTGACATTAA AAATTTCGTT TACTAACGTA GAAAGCGCTG ATAATTGAGG CTACTATTTT 421 AATATCTAGA AACAAAGATT CATTAATTAA TTAAGTAAGC TCCAGGATAC TTGTATATTT 481 CGTTAAAAAA GCGCAATTTC AACAATTCCT ATGAACATCC ATGATTTCAT CACAAAATCA 541 AACATATCAG GATTTAACGT GAATTATTTG CTTTCTTGTG ATTTTCATCT GATTTCTGGT 601 CATTTACGTT ACTGTAAACA AGCTTTTTAA AAAAAGGATT TTTTACGCAA TATTATCGAT 661 ATCCCAATCT GTAGTATAAA AAGTGCAACA GTGTTGAGTT TCCCGAAACC AGGCATTTTT 721 GCGGTAGGGT CTGAAACTTT CACGAAACCA TTTCACAACA AAACTTCTTC AACAACATGT 781 GTGCAAAGAA AATCGTCGTC TTACCAGGAG ACCATATTGG CCCTGAAATT GTTGCTTCTG 841 CCTTGGAGGT TTTGAAAGTC GTTGAGAAGA AGCGACCTGA GTTAAAACTC GAGTTTGAAG 901 AACACAAGAT TGGAGGTGCC TCTATTGATG CCTATGGAAC CCCTTTGACT GACGAGACTG 961 TGGAAGGCTT GTTTGGGAAG GCTGACGGGT GTCTTTTGGG TTGCGTTTGG TGGGTCTGGA 1021 ATGATACCCC AATGTCGGTC TGAGCCAAGT TTATTGAGCT TCGTAGAGTA TGGGGAGTAG 1081 GGTCAAACCC TTTG // |
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| Primer: ars_R1 | RDB14456_A6L7d.seq |
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>06183_14456_A6L7_ars_R1_G01_07_ABI08.ab1 1 TGGGTTTCGG TTTAATACTA AATTACTTAA AGTACCTTTA ACTAAACAAA ATGCCTATAT 61 ATATATTAAT TTACAATGAG TGTCAGATAA GTCACTATGT CCGAGTGGTT AAGGAGTTAG 121 ACTCGAATTC GCAAGCTTAG CTACAAATCC CACTGGCTAT ATGTATGCAT TTGTGTTAAA 181 AAAGTTTGTA TAGATTATTT AATCTACTCA GCATTCTTTC TCTAAATAGG AATTTGTTAC 241 TTAATGGAGA AAAAAATGTT TCGATTTACC TAGTGTATTT GTTTGTATAC TCACGTTTAA 301 TTTCAAACAT CCATTCTATC TTGTGTAATT TTTGGCATGG TGAAAAAGAT AATCAGCCTT 361 ATAATCTTTA CAAAAGTAAG AAATTCTGTA AATAAGCCTT AATGCCCTTG CTTTAAATTA 421 AAATGGTTCT TTTTCATGAT AATGTTTGCA CTTTGTGAAT ATATTTTAGA TAGTTCTGTG 481 AGGTATAATT AAGATGTTTT AGAGACTTAT ACAATTTTGT CTTTATAAAT TCTTAATTGA 541 TTTTACCATC CCAGTTTAAC TATGCTTCGT CGGCATCTCT GCACATGTCG TGTTTTCTTA 601 CCGTATTGTC CTACCAAGAA CCTCTTTTTT GCTTGGATCG AAATTAAAGG TTTAAAAGCA 661 AAGTTATGGA TGCTAGAGTA TTTCAAAGCT ATTCAGCTAG AGCTGAGGGG ATGAAAAAAT 721 CCCATTGCCA AGGAATTGTT GGCTTTGATG GAAGAAAAGC AAAGCAACTT GTCAGTCGCG 781 GTCGATTTGA CGAAGAAATC CGAAATCTTA GAATTGGGTA GATAAAATTG GGACCCTATG 841 TCTGTGTTAT CAAGACACAT ATTGACGTTG TCGAGGATTT CGACCAAGGA TATGGGTAGA 901 AAAAACTGGT GGCCTTAGGT AAAAAAGCAT CGTTTTCTTA TCCTTTGAAG GATCGCAAAT 961 TCGCCAGAAC AATTGGAAAA ATACCGGTCC AAGCTTACAA TAATGGCATC TGGGTGGTGT 1021 ACAAAAATTT GCCTTCTTGG GGCTCAATAA TCAAACAAAT TGCCAATAAC AGTGGCCAAG 1081 CCGAGGGTAT AATACCGAGG CCTTCAAAAG AAAAGCCTTG TGGT // |
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| Primer: f1ori_R3 | RDB14456_A6L7e.seq |
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>06183_14456_A6L7_f1ori_R3_E01_13_ABI24.ab1 1 GGCAGGGGAT GCTTGAGTAT GCGGTGTGAA TACCGCACAG ATGCGTAAGG AGAAAATACC 61 GCATCAGGCG CCATTCGCCA TTCAGGCTGC GCAACTGTTG GGAAGGGCGA TCGGTGCGGG 121 CCTCTTCGCT ATTACGCCAG CTGGCGAAAG GGGGATGTGC TGCAAGGCGA TTAAGTTGGG 181 TAACGCCAGG GTTTTCCCAG TCACGACGTT GTAAAACGAC GGCCAGTGAA TTCGAGCTCG 241 GGAGCGCTAC CGTGAATGGG CTCAACCAAA CAATGAACCT TTTCTTCTGA TTTTCCTACC 301 ACACCGGAAA GGGAGGCAGA AGGCAAAAGG CCCAAGCTAC CAGGAATGAC AGAAGCCTCA 361 TCTGAAATAA TGTCACCAAA CAAGTTGTCA GTCAAAACAA CACCGTTAAG TGTACGAGGG 421 CTCTTGACCA AAAGCATGGC TGCGGAGTCA ATGAGCTGGT TTTTTAAGGT AAGGTGAGGA 481 TATTCCTCCT TAAAAATCTT AGCTACAGTC TTGCGCCAAA GACGAGAAGT TGCCAAAACA 541 TTAGCTTTGT CGAGTAATGT GACGGGAGCA GGAGGGTTGG AAGTTTCAGC TAAGCGGCCG 601 CGGGCCCAAC CTTCCAATTC ATTAAATCGA GTCCTCCATC AGATGATTTA CAAGGTTTTG 661 CATAGAATCC CTAAGTAAGA GAAGTTGTTA GTGATTTCTT CAAGTTTACT AAAATTTACA 721 TACAAATGGA TGATCTCTTC TCCATTGTTT CCTTTCTTCT TGCAAACGCG TTTTACAAAG 781 AGATGACATG CTATTTTCTG ATTATTTTTT TCTATAGTTT TCTATTTTGT ACGTCCTTGG 841 GAGTCCGAAA TGTAAAATCG GGGTATGGTT AGTAGCGGCA GCGTTCATTA TGGTATATAA 901 AAGATAAAAA GTAGGGCAAG CGAGACAGAA TGGGATACAA GGGCATCGTC TATAATTATA 961 GCTAAAATTG TATTTTAATT TGTATTTTTT GTAATTTTTA TTTCTCATGT TTACTTAAAT 1021 GAATGCGATT AGAGAAAAAC TTTCACGAAT TGTCAAATAG CTCAGCAAAT ATACATTTTA 1081 GAAGCGAATC AGCACTTTAT TCTTCCGGTT GGTAGATTCG TTCGTCATAA ATTCGACATG 1141 TAACTGCAAT TTGGAAGTAC CCTCGGGATG // |
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Please visit Sequencing and PCR primers for primer information.
References
| original | Matsuyama, A., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913. |
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| original | Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162. |
| reference | Kitamura, K., Inhibition of the Arg/N-end rule pathway-mediated proteolysis by dipeptide-mimetic molecules. Amino Acids 48 (1): 235-243 ((2016). PMID 26334349. |
| reference | Kitamura, K., The type-2 N-end rule peptide recognition activity of Ubr11 ubiquitin ligase is required for the expression of peptide transporters. FEBS Lett. 587 (2): 214-219 (2013). PMID 23219921. |
2018.12.10
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