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pDUAL-HFG41

S. pombe 5 ter end His6-FLAG-GFP(S65C)-tagged vector with nmt1 (medium strength) promoter

Catalog number RDB06183
Resource name pDUAL-HFG41
Clone info. S. pombe 5 ter end His6-FLAG-GFP(S65C)-tagged vector with nmt1 (medium strength) promoter.
Multicopy marker, ura4+; Integration marker, leu1+. Gateway incompatible.
Vector backbone pDUAL (Plasmid)
Selectable markers Amp^r
Reference of nucleotide sequence AB364050.1 (DDBJ accession number)
Depositor Yoshida, Minoru | Matsuyama, Akihisa |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
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Material Transfer Agreement (MTA) [Word]
Schedule A (GFP-A) [Link]
Remarks ((Additional form)) GFP-A
Please use Schedule A (GFP-A) additionally.
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RDB06183 pDUAL-HFG41 DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB06183z.seq from Depositor

Featured content

Featured content Expression Vector Backbone of Schizosaccharomyces pombe (English text)

References

original Matsuyama, A., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913.
original Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB14456_A6L7p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: nmt1Fw RDB14456_A6L7a.seq
>06183_14456_A6L7_nmt1Fw_D01_04_ABI08.ab1
    1 CAAAAATTTA TTAAATCAGG AAAACGTAAC TCTCGGCTAC TGGATGGTTC AGTCACCCAA
   61 CGATTACTGG GGAGAGAAAA CAGGGCAAAA GCAAAGCTTA AAGGAATCCG ATTGTCATTC
  121 GGCAATGTGC AGCGAAACTA AAAACCGGAT AATGGACCTG TTAATCGAAA CATTGAAGAT
  181 AAAGGAAGAG GAATCCTGGC ATATCATCAA TTGAATAAGT TGAATTAATT ATTTCAATCT
  241 CATTCTCACT TTCTGACTTA TAGTCGCTTT GTTAAAGCTA GCGTCGACAG GCCTGGATCC
  301 TATGCATCAC CACCATCATC ATATGGATTA TAAGGATGAT GACGATAAAC CCATGGGTAA
  361 AGGAGAAGAA CTTTTCACTG GAGTTGTCCC AATTCTTGTT GAATTAGATG GTGATGTTAA
  421 TGGGCACAAA TTTTCTGTCA GTGGAGAGGG TGAAGGTGAT GCAACATACG GAAAACTTAC
  481 CCTTAAATTT ATTTGCACTA CTGGAAAACT ACCTGTTCCG TGGCCAACAC TTGTCACTAC
  541 TTTCTGTTAT GGTGTTCAAT GCTTTTCAAG ATACCCAGAT CACATGAAAC AGCATGACTT
  601 TTTCAAGAGT GCCATGCCCG AAGGCTATGT ACAGGAAAGA ACTATATTTT TCAAAGATGA
  661 CGGGAACTAC AAGACACGTG CTGAAGTCAA GTTTGAAGGT GATACCCTTG TTAATAGAAT
  721 CGAGTTAAAA GGTATTGATT TTAAAGAAGA TGGAAACATT CTTGGACACA AATTGGAATA
  781 CAACTATAAC TCACACAATG TATACATCAT GGCAGACAAA CAAAAGAATG GAATCAAAGT
  841 TAACTTCAAA ATTAGACACA ACATTGAAGA TGGAAGCGTT TCAACTAGCA GACCATTATC
  901 AACAAAATAC TCCAAATTGG CGATGGCCCT GTCCTTTTAC CAGACAAACC ATTACCCTGT
  961 CCACACAATC TGCCTTTTCG AAAGATCCCA CCGAAAAGAG AGACACAATG GTTCGTTCCT
 1021 GAGTTTGGTA CAGCTGGCTT GGAATTACAC ATTGGCATGG AATGAACTTA TACACGATAT
1081 CATGAAATCT CGAACCTCTA GAGGAATCG
//
Primer: ADH_rev RDB14456_A6L7b.seq
>06183_14456_A6L7_ADH_rev_E01_05_ABI08.ab1
    1 GGAAGGAGTT CCTCAGGATA GGAATTTTCG TTTTAAAACC TAAGAGTCAC TTTAAAATTT
   61 GTATACACTT ATTTTTTTTA TAACTTATTT AATAATAAAA ATCATAAATC ATAAGAAATT
  121 CGCTTATTTA GAAGTGGCGC GCCGGATCCT CTAGAGTCGA GATCTCATGA TATCTTTGTA
  181 TAGTTCATCC ATGCCATGTG TAATCCCAGC AGCTGTTACA AACTCAAGAA GGACCATGTG
  241 GTCTCTCTTT TCGTTGGGAT CTTTCGAAAG GGCAGATTGT GTGGACAGGT AATGGTTGTC
  301 TGGTAAAAGG ACAGGGCCAT CGCCAATTGG AGTATTTTGT TGATAATGGT CTGCTAGTTG
  361 AACGCTTCCA TCTTCAATGT TGTGTCTAAT TTTGAAGTTA ACTTTGATTC CATTCTTTTG
  421 TTTGTCTGCC ATGATGTATA CATTGTGTGA GTTATAGTTG TATTCCAATT TGTGTCCAAG
  481 AATGTTTCCA TCTTCTTTAA AATCAATACC TTTTAACTCG ATTCTATTAA CAAGGGTATC
  541 ACCTTCAAAC TTGACTTCAG CACGTGTCTT GTAGTTCCCG TCATCTTTGA AAAATATAGT
  601 TCTTTCCTGT ACATAGCCTT CGGGCATGGC ACTCTTGAAA AAGTCATGCT GTTTCATGTG
  661 ATCTGGGTAT CTTGAAAAGC ATTGAACACC ATAACAGAAA GTAGTGACAA GTGTTGGCCA
  721 CGGAACAGGT AGTTTTCCAG TAGTGCAAAT AAATTTAAGG GTAAGTTTTC CGTATGTTGC
  781 ATCACCTTCA CCCTCTCCAC TGACAGAAAA TTTGTGCCCA TTAACATCAC CATCTAATTC
  841 AACAAGAAAT TGGGACAACT CCAGTGAAAA GTTCTTCTCC TTTACCCATG GGTTTATCGT
  901 CATCATCCTT ATAATCCATA TGATGATGGT GGTGATGCAT AGGATCCAGG CCCTGTCGAC
  961 GCTAGCTTTA ACAAAGCGAC TATAAGTCAG AAAGTGAGAT GAGATTGAAA TAGTAAATCA
 1021 CTTATTCAAA TTGAATGAAT ATGGCCAGGA TTTCCTTCGT GCGTAATCCT CAATGTTTCG
1081 AATTAAACCA GGTCCCCAGA T
//
Primer: ADH-Forward RDB14456_A6L7c.seq
>06183_14456_A6L7_ADH-Forward_F01_06_ABI08.ab1
    1 TATCCCCTTT TTTTGACACA CCTCTACCGG CAGATCCGCT AGGGATAACA GGGTAATATA
   61 CTAGTAAGAT GATTCTTTTA TCGTCGATAA CGATGAGGAT GTAGACGATT ATGATGAGAG
  121 TGATTAGCTT CTAAAATTTG GTGTTTTCAA ACGCTATTTA CAGTACAATC TGAAAAAATT
  181 GGATGAACAT TGTAAATGGT AGGATACGAG TTCCAATTAA TGTATTTTTT AAGTATTACA
  241 CTTAGCTTAT TAATTTTATA TATACATTCT ACCTCTGTGG GTTTAAAGAC AACTCGTACA
  301 CATACTCAAA TTATTTATGA ACATTATAAT GCATATTCGT ACTGTCTATT AAACATTTTT
  361 TTGACATTAA AAATTTCGTT TACTAACGTA GAAAGCGCTG ATAATTGAGG CTACTATTTT
  421 AATATCTAGA AACAAAGATT CATTAATTAA TTAAGTAAGC TCCAGGATAC TTGTATATTT
  481 CGTTAAAAAA GCGCAATTTC AACAATTCCT ATGAACATCC ATGATTTCAT CACAAAATCA
  541 AACATATCAG GATTTAACGT GAATTATTTG CTTTCTTGTG ATTTTCATCT GATTTCTGGT
  601 CATTTACGTT ACTGTAAACA AGCTTTTTAA AAAAAGGATT TTTTACGCAA TATTATCGAT
  661 ATCCCAATCT GTAGTATAAA AAGTGCAACA GTGTTGAGTT TCCCGAAACC AGGCATTTTT
  721 GCGGTAGGGT CTGAAACTTT CACGAAACCA TTTCACAACA AAACTTCTTC AACAACATGT
  781 GTGCAAAGAA AATCGTCGTC TTACCAGGAG ACCATATTGG CCCTGAAATT GTTGCTTCTG
  841 CCTTGGAGGT TTTGAAAGTC GTTGAGAAGA AGCGACCTGA GTTAAAACTC GAGTTTGAAG
  901 AACACAAGAT TGGAGGTGCC TCTATTGATG CCTATGGAAC CCCTTTGACT GACGAGACTG
  961 TGGAAGGCTT GTTTGGGAAG GCTGACGGGT GTCTTTTGGG TTGCGTTTGG TGGGTCTGGA
 1021 ATGATACCCC AATGTCGGTC TGAGCCAAGT TTATTGAGCT TCGTAGAGTA TGGGGAGTAG
1081 GGTCAAACCC TTTG
//
Primer: ars_R1 RDB14456_A6L7d.seq
>06183_14456_A6L7_ars_R1_G01_07_ABI08.ab1
    1 TGGGTTTCGG TTTAATACTA AATTACTTAA AGTACCTTTA ACTAAACAAA ATGCCTATAT
   61 ATATATTAAT TTACAATGAG TGTCAGATAA GTCACTATGT CCGAGTGGTT AAGGAGTTAG
  121 ACTCGAATTC GCAAGCTTAG CTACAAATCC CACTGGCTAT ATGTATGCAT TTGTGTTAAA
  181 AAAGTTTGTA TAGATTATTT AATCTACTCA GCATTCTTTC TCTAAATAGG AATTTGTTAC
  241 TTAATGGAGA AAAAAATGTT TCGATTTACC TAGTGTATTT GTTTGTATAC TCACGTTTAA
  301 TTTCAAACAT CCATTCTATC TTGTGTAATT TTTGGCATGG TGAAAAAGAT AATCAGCCTT
  361 ATAATCTTTA CAAAAGTAAG AAATTCTGTA AATAAGCCTT AATGCCCTTG CTTTAAATTA
  421 AAATGGTTCT TTTTCATGAT AATGTTTGCA CTTTGTGAAT ATATTTTAGA TAGTTCTGTG
  481 AGGTATAATT AAGATGTTTT AGAGACTTAT ACAATTTTGT CTTTATAAAT TCTTAATTGA
  541 TTTTACCATC CCAGTTTAAC TATGCTTCGT CGGCATCTCT GCACATGTCG TGTTTTCTTA
  601 CCGTATTGTC CTACCAAGAA CCTCTTTTTT GCTTGGATCG AAATTAAAGG TTTAAAAGCA
  661 AAGTTATGGA TGCTAGAGTA TTTCAAAGCT ATTCAGCTAG AGCTGAGGGG ATGAAAAAAT
  721 CCCATTGCCA AGGAATTGTT GGCTTTGATG GAAGAAAAGC AAAGCAACTT GTCAGTCGCG
  781 GTCGATTTGA CGAAGAAATC CGAAATCTTA GAATTGGGTA GATAAAATTG GGACCCTATG
  841 TCTGTGTTAT CAAGACACAT ATTGACGTTG TCGAGGATTT CGACCAAGGA TATGGGTAGA
  901 AAAAACTGGT GGCCTTAGGT AAAAAAGCAT CGTTTTCTTA TCCTTTGAAG GATCGCAAAT
  961 TCGCCAGAAC AATTGGAAAA ATACCGGTCC AAGCTTACAA TAATGGCATC TGGGTGGTGT
 1021 ACAAAAATTT GCCTTCTTGG GGCTCAATAA TCAAACAAAT TGCCAATAAC AGTGGCCAAG
1081 CCGAGGGTAT AATACCGAGG CCTTCAAAAG AAAAGCCTTG TGGT
//
Primer: f1ori_R3 RDB14456_A6L7e.seq
>06183_14456_A6L7_f1ori_R3_E01_13_ABI24.ab1
    1 GGCAGGGGAT GCTTGAGTAT GCGGTGTGAA TACCGCACAG ATGCGTAAGG AGAAAATACC
   61 GCATCAGGCG CCATTCGCCA TTCAGGCTGC GCAACTGTTG GGAAGGGCGA TCGGTGCGGG
  121 CCTCTTCGCT ATTACGCCAG CTGGCGAAAG GGGGATGTGC TGCAAGGCGA TTAAGTTGGG
  181 TAACGCCAGG GTTTTCCCAG TCACGACGTT GTAAAACGAC GGCCAGTGAA TTCGAGCTCG
  241 GGAGCGCTAC CGTGAATGGG CTCAACCAAA CAATGAACCT TTTCTTCTGA TTTTCCTACC
  301 ACACCGGAAA GGGAGGCAGA AGGCAAAAGG CCCAAGCTAC CAGGAATGAC AGAAGCCTCA
  361 TCTGAAATAA TGTCACCAAA CAAGTTGTCA GTCAAAACAA CACCGTTAAG TGTACGAGGG
  421 CTCTTGACCA AAAGCATGGC TGCGGAGTCA ATGAGCTGGT TTTTTAAGGT AAGGTGAGGA
  481 TATTCCTCCT TAAAAATCTT AGCTACAGTC TTGCGCCAAA GACGAGAAGT TGCCAAAACA
  541 TTAGCTTTGT CGAGTAATGT GACGGGAGCA GGAGGGTTGG AAGTTTCAGC TAAGCGGCCG
  601 CGGGCCCAAC CTTCCAATTC ATTAAATCGA GTCCTCCATC AGATGATTTA CAAGGTTTTG
  661 CATAGAATCC CTAAGTAAGA GAAGTTGTTA GTGATTTCTT CAAGTTTACT AAAATTTACA
  721 TACAAATGGA TGATCTCTTC TCCATTGTTT CCTTTCTTCT TGCAAACGCG TTTTACAAAG
  781 AGATGACATG CTATTTTCTG ATTATTTTTT TCTATAGTTT TCTATTTTGT ACGTCCTTGG
  841 GAGTCCGAAA TGTAAAATCG GGGTATGGTT AGTAGCGGCA GCGTTCATTA TGGTATATAA
  901 AAGATAAAAA GTAGGGCAAG CGAGACAGAA TGGGATACAA GGGCATCGTC TATAATTATA
  961 GCTAAAATTG TATTTTAATT TGTATTTTTT GTAATTTTTA TTTCTCATGT TTACTTAAAT
 1021 GAATGCGATT AGAGAAAAAC TTTCACGAAT TGTCAAATAG CTCAGCAAAT ATACATTTTA
 1081 GAAGCGAATC AGCACTTTAT TCTTCCGGTT GGTAGATTCG TTCGTCATAA ATTCGACATG
1141 TAACTGCAAT TTGGAAGTAC CCTCGGGATG
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Matsuyama, A., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913.
original Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162.
reference Kitamura, K., Inhibition of the Arg/N-end rule pathway-mediated proteolysis by dipeptide-mimetic molecules. Amino Acids 48 (1): 235-243 ((2016). PMID 26334349.
reference Kitamura, K., The type-2 N-end rule peptide recognition activity of Ubr11 ubiquitin ligase is required for the expression of peptide transporters. FEBS Lett. 587 (2): 214-219 (2013). PMID 23219921.

2018.12.10

GNP_filter3_RDBDEP_html_181012.pl