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pDUAL-HFG1

S. pombe 5 ter end His6-FLAG-GFP(S65C)-tagged vector with nmt1 promoter

Catalog number RDB06182
Resource name pDUAL-HFG1
Clone info. S. pombe 5 ter end His6-FLAG-GFP(S65C)-tagged vector with nmt1 promoter.
Multicopy marker, ura4+; Integration marker, leu1+. Gateway incompatible.
Vector backbone pDUAL (Plasmid)
Selectable markers Amp^r
Reference of nucleotide sequence AB364049.1 (DDBJ accession number)
Depositor Yoshida, Minoru | Matsuyama, Akihisa |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
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Material Transfer Agreement (MTA) [Word]
Schedule A (GFP-A) [Link]
Remarks ((Additional form)) GFP-A
Please use Schedule A (GFP-A) additionally.
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB06182 pDUAL-HFG1 DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB06182z.seq from Depositor

Featured content

Featured content Expression Vector Backbone of Schizosaccharomyces pombe (English text)

References

original Matsuyama, A., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913.
original Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB13522_A5Ipp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: nmt1Fw RDB13522_A5Ipa.seq
>06182_13522_A5Ip_nmt1Fw_D04_10.ab1
    1 AGGAATTTTA TTAAATCAGG AAAAACGTAA CTCTCGGCTA CTGGATGGTT CAGTCACCCA
   61 ACGATTACTG GGGAGAGAAA ACAGGGCAAA AGCAAAGCTT AAAGGAATCC GATTGTCATT
  121 CGGCAATGTG CAGCGAAACT AAAAACCGGA TAATGGACCT GTTAATCGAA ACATTGAAGA
  181 TATATAAAGG AAGAGGAATC CTGGCATATC ATCAATTGAA TAAGTTGAAT TAATTATTTC
  241 AATCTCATTC TCACTTTCTG ACTTATAGTC GCTTTGTTAA AGCTAGCGTC GACAGGCCTG
  301 GATCCTATGC ATCACCACCA TCATCATATG GATTATAAGG ATGATGACGA TAAACCCATG
  361 GGTAAAGGAG AAGAACTTTT CACTGGAGTT GTCCCAATTC TTGTTGAATT AGATGGTGAT
  421 GTTAATGGGC ACAAATTTTC TGTCAGTGGA GAGGGTGAAG GTGATGCAAC ATACGGAAAA
  481 CTTACCCTTA AATTTATTTG CACTACTGGA AAACTACCTG TTCCGTGGCC AACACTTGTC
  541 ACTACTTTCT GTTATGGTGT TCAATGCTTT TCAAGATACC CAGATCACAT GAAACAGCAT
  601 GACTTTTTCA AGAGTGCCAT GCCCGAAGGC TATGTACAGG AAAGAACTAT ATTTTTCAAA
  661 GATGACGGGA ACTACAAGAC ACGTGCTGAA GTCAAGTTTG AAGGTGATAC CCTTGTTAAT
  721 AGAATCGAGT TAAAAGGTAT TGATTTTAAA GAAGATGGAA ACATTCTTGG ACACAAATTG
  781 GAATACAACT ATAACTCACA CAATGTATAC ATCATGGCAG ACAAACAAAA GAATGGAATC
  841 AAAGTTAACT TCAAAATTAG ACACAACATT GAAGATGGAA GCGTTCAACT AGCAGACCAT
  901 TATCAACAAA ATACTCCAAT TGGCGATGGC CCTGTCCTTT TACCAGACAA CCATTACCTG
  961 TCCACACAAT CTGCCCTTTC GAAAGATCCC AACGAAAAGA GAGACCACAT GGGTCCTTCT
 1021 TGAGTTTGTA ACAGCTGCTG GGATTTACAC ATGGCATGGG ATGAACTATA CAAAGGATAT
 1081 CATGAGATCT CGACTCTAGA GGATCCGGCG CGCCAACTTC TAATAGCCGA TTCCTAATGG
1141 ATTTATTGAA TTTTATTATT ACATTAGTTA ATTAAAAAAT TAGAGCGT
//
Primer: ADH_rev RDB13522_A5Ipb.seq
>06182_13522_A5Ip_ADHrev_E04_13.ab1
    1 TAAAGAAATT CTTCAGGAAA TAAGAATTTT CGTTTTAAAA CCTAAGAGTC ACTTTAAAAT
   61 TTGTATACAC TTATTTTTTT TATAACTTAT TTAATAATAA AAATCATAAA TCATAAGAAA
  121 TTCGCTTATT TAGAAGTGGC GCGCCGGATC CTCTAGAGTC GAGATCTCAT GATATCTTTG
  181 TATAGTTCAT CCATGCCATG TGTAATCCCA GCAGCTGTTA CAAACTCAAG AAGGACCATG
  241 TGGTCTCTCT TTTCGTTGGG ATCTTTCGAA AGGGCAGATT GTGTGGACAG GTAATGGTTG
  301 TCTGGTAAAA GGACAGGGCC ATCGCCAATT GGAGTATTTT GTTGATAATG GTCTGCTAGT
  361 TGAACGCTTC CATCTTCAAT GTTGTGTCTA ATTTTGAAGT TAACTTTGAT TCCATTCTTT
  421 TGTTTGTCTG CCATGATGTA TACATTGTGT GAGTTATAGT TGTATTCCAA TTTGTGTCCA
  481 AGAATGTTTC CATCTTCTTT AAAATCAATA CCTTTTAACT CGATTCTATT AACAAGGGTA
  541 TCACCTTCAA ACTTGACTTC AGCACGTGTC TTGTAGTTCC CGTCATCTTT GAAAAATATA
  601 GTTCTTTCCT GTACATAGCC TTCGGGCATG GCACTCTTGA AAAAGTCATG CTGTTTCATG
  661 TGATCTGGGT ATCTTGAAAA GCATTGAACA CCATAACAGA AAGTAGTGAC AAGTGTTGGC
  721 CACGGAACAG GTAGTTTTCC AGTAGTGCAA ATAAATTTAA GGGTAAGTTT TCCGTATGTT
  781 GCATCACCTT CACCCTCTCC ACTGACAGAA AATTTGTGCC CATTAACATC ACCATCTAAT
  841 TCAACAAGAA TTGGGACAAC TCCAGTGAAA AGTTCTTCTC CTTTACCCAT GGGTTTATCG
  901 TCATCATCCT TATAATCCAA TATGATGATG GTGGTGAATG CATAGGATCC AGGCCTGTCG
  961 ACGCTAGCTT TAACAAAGCG GACTTATAAG TCAGAAGTGA GATGAGATTG AAATAATTTA
 1021 TTCCAACTTA ATTCCAATGG ATGATTGGCA GGGATTCCTC TCCTTATATA TATCCTCATG
 1081 GTTCGATACC GTCCATATCC GTTTACTCGC TGCAATGCGA TGCATCGGAT CCTAGCTTGC
1141 TTTGCCTGGA TTTCTCTCCC CG
//
Primer: ADH-Forward RDB13522_A5Ipc.seq
>06182_13522_A5Ip_ADH-Forward_F04_16.ab1
    1 TATTCTTTAT TGACACACCT CTACCGGCAG ATCCGCTAGG GATAACAGGG TAATATACTA
   61 GTAAGATGAT TCTTTTATCG TCGATAACGA TGAGGATGTA GACGATTATG ATGAGAGTGA
  121 TTAGCTTCTA AAATTTGGTG TTTTCAAACG CTATTTACAG TACAATCTGA AAAAATTGGA
  181 TGAACATTGT AAATGGTAGG ATACGAGTTC CAATTAATGT ATTTTTTAAG TATTACACTT
  241 AGCTTATTAA TTTTATATAT ACATTCTACC TCTGTGGGTT TAAAGACAAC TCGTACACAT
  301 ACTCAAATTA TTTATGAACA TTATAATGCA TATTCGTACT GTCTATTAAA CATTTTTTTG
  361 ACATTAAAAA TTTCGTTTAC TAACGTAGAA AGCGCTGATA ATTGAGGCTA CTATTTTAAT
  421 ATCTAGAAAC AAAGATTCAT TAATTAATTA AGTAAGCTCC AGGATACTTG TATATTTCGT
  481 TAAAAAAGCG CAATTTCAAC AATTCCTATG AACATCCATG ATTTCATCAC AAAATCAAAC
  541 ATATCAGGAT TTAACGTGAA TTATTTGCTT TCTGTGGATT TTCATCTGAT TTCTGGGTCA
  601 TTACGTTACT GTAAACAAGC TTTTAAAAAA AAGGATTTTT TACGCAATTA TTATCGATAT
  661 TCCGATCTGT AGTATAAAAA AGTTGGCAAC AGTGTGTGAG TTTCCGGAAA CAGGCATTTT
  721 TGCCGTTAGG TCTGGAAACT TTCCCGGAAA CATTCACAAC AAAAACTTCT TCACACATTG
  781 GTTGGCCAAG GAAATCTGTC GTTCTTACCA GAGACATATT GGCCTTGAAA TGTGCTCGGC
  841 TGAGATTGGA AGTCGTGAAG AACGACGGAT GAACTCAGTT GGACCAGTTG AGGTCCCTTT
901 TAGGACTTTG GACCTGATGA GCAAA
//
Primer: ars_R1 RDB13522_A5Ipd.seq
>06182_13522_A5Ip_ars_R1_G04_19.ab1
    1 AGGTTTTTGG TTTATACTAA TTTACTTAAG TACCTTTAAC TAAACAAAAT GCCTATATAT
   61 ATATTAATTT ACAATGAGTG TCACATAAGT CACTATGTCC GAGTGGTTAA GGAGTTAGAC
  121 TCGAATTCGC AAGCTTAGCT ACAAATCCCA CTGGCTATAT GTATGCATTT GTGTTAAAAA
  181 AGTTTGTATA GATTATTTAA TCTACTCAGC ATTCTTTCTC TAAATAGGAA TTTGTTACTT
  241 AATGGAGAAA AAAATGTTTC GATTTACCTA GTGTATTTGT TTGTATACTC ACGTTTAATT
  301 TCAAACATCC ATTCTATCTT GTGTAATTTT TGGCATGGTG AAAAAGATAA TCAGCCTTAT
  361 AATCTTTACA AAAGTAAGAA ATTCTGTAAA TAAGCCTTAA TGCCCTTGCT TTAAATTAAA
  421 ATGGTTCTTT TTCATGATAA TGTTTGCACT TTGTGAATAT ATTTTAGATA GTTCTGTGAG
  481 GTATAATTAA GATGTTTTAG AGACTTATAC AATTTTGTCT TTATAAATTC TTAATTGATT
  541 TTACCATCCC AGTTTAACTA TGCTTCGTCG GCATCTCTGC ACATGTCGTG TTTTCTTACC
  601 GTATTGTCCT ACCAAGAACC TCTTTTTTGC TTGGATCGAA ATTAAAGGTT TAAAAGCAAA
  661 GTTATGGATG CTAGAGTATT TCAAAGCTAT TCAGCTAGAG CTGAGGGGAT GAAAAATCCC
  721 ATTGCCAAGG AATTGTTGGC TTTGATGGAA GAAAAGCAAA GCAACTTGTC AGTCGCGGTC
  781 GATTTGACGA AGAAATCCGA AATCTTAGAA ATTGGTAGAT AAAATTGGAC CCTATGTCTG
  841 TGTTATCAAG ACACATATTG AACGTTGTCG AGGATTTCGA CCAGGATATG GTAGAAAAAA
  901 CTGGTGGCCT TAGGTAAAAA GCATCGTTTT CTTATCTTTG AGGATCGCAA ATTCCGCAGG
  961 ACATTGGAAA TACCGTCAAG CTACAATATG CATCTGGGTG TGTACAAAAT TGCTTCTTGG
 1021 GCTCATATCA CAAATGGCCA TACAGTGCCA GGCCGAGGGT ATTATACAAG GCCTCCAAAG
 1081 AAGCTGGGTT TACCTTTGGG GACGTGGTCC TCATGCTTTG GCTGAATGGC CTTCCAAGGC
 1141 TCTTTGCTAA CTGTCTACAC AGAGGAATCT TAGAAATGGG TTGGGAGAAT GCATCATCCC
1201 GCGACAATTT TTTG
//
Primer: f1ori_R3 RDB13522_A5Ipe.seq
>06182_13522_A5Ip_f1ori_R3_H04_22.ab1
    1 GCGGGTGGGG GCTTTCGTAT GCGGTGTGAA ATACCGCACA GATGCGTAAG GAGAAAATAC
   61 CGCATCAGGC GCCATTCGCC ATTCAGGCTG CGCAACTGTT GGGAAGGGCG ATCGGTGCGG
  121 GCCTCTTCGC TATTACGCCA GCTGGCGAAA GGGGGATGTG CTGCAAGGCG ATTAAGTTGG
  181 GTAACGCCAG GGTTTTCCCA GTCACGACGT TGTAAAACGA CGGCCAGTGA ATTCGAGCTC
  241 GGGAGCGCTA CCGTGAATGG GCTCAACCAA ACAATGAACC TTTTCTTCTG ATTTTCCTAC
  301 CACACCGGAA AGGGAGGCAG AAGGCAAAAG GCCCAAGCTA CCAGGAATGA CAGAAGCCTC
  361 ATCTGAAATA ATGTCACCAA ACAAGTTGTC AGTCAAAACA ACACCGTTAA GTGTACGAGG
  421 GCTCTTGACC AAAAGCATGG CTGCGGAGTC AATGAGCTGG TTTTTTAAGG TAAGGTGAGG
  481 ATATTCCTCC TTAAAAATCT TAGCTACAGT CTTGCGCCAA AGACGAGAAG TTGCCAAAAC
  541 ATTAGCTTTG TCGAGTAATG TGACGGGAGC AGGAGGGTTG GAAGTTTCAG CTAAGCGGCC
  601 GCGGGCCCAA CCTTCCAATT CATTAAATCG AGTCCTCCAT CAGATGATTT ACAAGGTTTT
  661 GCATAGAATC CCTAAGTAAG AGAAGTTGTT AGTGATTTCT TCAAGTTTAC TAAAATTTAC
  721 ATACAAATGG ATGATCTCTT CTCCATTGTT TCCTTTCTTC TTGCAAACGC GTTTTACAAA
  781 GAGATGACAT GCTATTTTCT GATTATTTTT TTCTATAGTT TTCTATTTTG TACGTCCTTG
  841 GGAGTCCGAA ATGTAAAATC GGGGTATGGT TAGTAGCGGC AGCGTTCATT ATGGTATATA
  901 AAAGATAAAA AGTAGGGGCA AGCGAGACAG AATGGGATAC AAGGGCATCG TCTATAATTA
  961 TAGCTAAAAA TTGTATTTTA ATTTGTATTT TTTTGTAATT TTATTTCTCA TTGTTTTACT
 1021 TTAAATGAAA TGCGAAATTA GAGAAAACTT CCAACGGAAA TGTCAAAATA AGCTCAGCAA
 1081 AAATAATAAC AATTTTACGA AAGCGATCAG CAACTTATTT CTCCGCTGGT AGATTCCTTT
1141 TCCTCATAAA TCAACAATGT ACCTGCCAAT TGGAGTTTTC CCTCCATAGA G
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Matsuyama, A., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913.
original Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162.

2018.12.10

GNP_filter3_RDBDEP_html_181012.pl