RDB06182 - pDUAL-HFG1

Resource data sheet
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pDUAL-HFG1

S. pombe 5 ter end His6-FLAG-GFP(S65C)-tagged vector with nmt1 promoter

Catalog number	RDB06182
Resource name	pDUAL-HFG1
Clone info.	S. pombe 5 ter end His6-FLAG-GFP(S65C)-tagged vector with nmt1 promoter. Multicopy marker, ura4+; Integration marker, leu1+. Gateway incompatible.
Vector backbone	pDUAL (Plasmid)
Selectable markers	Amp^r
Reference of nucleotide sequence	AB364049.1 (DDBJ accession number)
Depositor	Yoshida, Minoru \| Matsuyama, Akihisa \|

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.

Ordering Please visit Information of Request for Distribution.
Order form [Credit Card Payment] [Bank Transfer or Check Payment]
Material Transfer Agreement (MTA) [Word]
Schedule A (GFP-A) [Link]

Remarks ((Additional form)) GFP-A
Please use Schedule A (GFP-A) additionally.

Distribution information (domestic users) [open/close]

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
Ordering	Please visit Information of Request for Distribution. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word] Schedule A (GFP-A) [Link]
Remarks	((Additional form)) GFP-A Please use Schedule A (GFP-A) additionally.

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献 (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) の引用と、　寄託者への謝辞の表明を必要とする。　利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。　寄託者から要請がある場合、理研ＢＲＣは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。
提供依頼	手続きの詳細は、「提供申込みについて」をご覧ください。提供依頼書 [Word] 提供同意書 [Word] Schedule A（GFP-A, 英語版のみ） [Link]
備考	【追加提供依頼書】GFP-A Schedule A（GFP-A, 英語版のみ）が追加で必要です。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB06182	pDUAL-HFG1	DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file	Sequence RDB06182z.seq from Depositor

Featured content	Expression Vector Backbone of Schizosaccharomyces pombe (English text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet	RDB13522_A5Ipp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: nmt1Fw	RDB13522_A5Ipa.seq
>06182_13522_A5Ip_nmt1Fw_D04_10.ab1 1 AGGAATTTTA TTAAATCAGG AAAAACGTAA CTCTCGGCTA CTGGATGGTT CAGTCACCCA 61 ACGATTACTG GGGAGAGAAA ACAGGGCAAA AGCAAAGCTT AAAGGAATCC GATTGTCATT 121 CGGCAATGTG CAGCGAAACT AAAAACCGGA TAATGGACCT GTTAATCGAA ACATTGAAGA 181 TATATAAAGG AAGAGGAATC CTGGCATATC ATCAATTGAA TAAGTTGAAT TAATTATTTC 241 AATCTCATTC TCACTTTCTG ACTTATAGTC GCTTTGTTAA AGCTAGCGTC GACAGGCCTG 301 GATCCTATGC ATCACCACCA TCATCATATG GATTATAAGG ATGATGACGA TAAACCCATG 361 GGTAAAGGAG AAGAACTTTT CACTGGAGTT GTCCCAATTC TTGTTGAATT AGATGGTGAT 421 GTTAATGGGC ACAAATTTTC TGTCAGTGGA GAGGGTGAAG GTGATGCAAC ATACGGAAAA 481 CTTACCCTTA AATTTATTTG CACTACTGGA AAACTACCTG TTCCGTGGCC AACACTTGTC 541 ACTACTTTCT GTTATGGTGT TCAATGCTTT TCAAGATACC CAGATCACAT GAAACAGCAT 601 GACTTTTTCA AGAGTGCCAT GCCCGAAGGC TATGTACAGG AAAGAACTAT ATTTTTCAAA 661 GATGACGGGA ACTACAAGAC ACGTGCTGAA GTCAAGTTTG AAGGTGATAC CCTTGTTAAT 721 AGAATCGAGT TAAAAGGTAT TGATTTTAAA GAAGATGGAA ACATTCTTGG ACACAAATTG 781 GAATACAACT ATAACTCACA CAATGTATAC ATCATGGCAG ACAAACAAAA GAATGGAATC 841 AAAGTTAACT TCAAAATTAG ACACAACATT GAAGATGGAA GCGTTCAACT AGCAGACCAT 901 TATCAACAAA ATACTCCAAT TGGCGATGGC CCTGTCCTTT TACCAGACAA CCATTACCTG 961 TCCACACAAT CTGCCCTTTC GAAAGATCCC AACGAAAAGA GAGACCACAT GGGTCCTTCT 1021 TGAGTTTGTA ACAGCTGCTG GGATTTACAC ATGGCATGGG ATGAACTATA CAAAGGATAT 1081 CATGAGATCT CGACTCTAGA GGATCCGGCG CGCCAACTTC TAATAGCCGA TTCCTAATGG 1141 ATTTATTGAA TTTTATTATT ACATTAGTTA ATTAAAAAAT TAGAGCGT //
Primer: ADH_rev	RDB13522_A5Ipb.seq
>06182_13522_A5Ip_ADHrev_E04_13.ab1 1 TAAAGAAATT CTTCAGGAAA TAAGAATTTT CGTTTTAAAA CCTAAGAGTC ACTTTAAAAT 61 TTGTATACAC TTATTTTTTT TATAACTTAT TTAATAATAA AAATCATAAA TCATAAGAAA 121 TTCGCTTATT TAGAAGTGGC GCGCCGGATC CTCTAGAGTC GAGATCTCAT GATATCTTTG 181 TATAGTTCAT CCATGCCATG TGTAATCCCA GCAGCTGTTA CAAACTCAAG AAGGACCATG 241 TGGTCTCTCT TTTCGTTGGG ATCTTTCGAA AGGGCAGATT GTGTGGACAG GTAATGGTTG 301 TCTGGTAAAA GGACAGGGCC ATCGCCAATT GGAGTATTTT GTTGATAATG GTCTGCTAGT 361 TGAACGCTTC CATCTTCAAT GTTGTGTCTA ATTTTGAAGT TAACTTTGAT TCCATTCTTT 421 TGTTTGTCTG CCATGATGTA TACATTGTGT GAGTTATAGT TGTATTCCAA TTTGTGTCCA 481 AGAATGTTTC CATCTTCTTT AAAATCAATA CCTTTTAACT CGATTCTATT AACAAGGGTA 541 TCACCTTCAA ACTTGACTTC AGCACGTGTC TTGTAGTTCC CGTCATCTTT GAAAAATATA 601 GTTCTTTCCT GTACATAGCC TTCGGGCATG GCACTCTTGA AAAAGTCATG CTGTTTCATG 661 TGATCTGGGT ATCTTGAAAA GCATTGAACA CCATAACAGA AAGTAGTGAC AAGTGTTGGC 721 CACGGAACAG GTAGTTTTCC AGTAGTGCAA ATAAATTTAA GGGTAAGTTT TCCGTATGTT 781 GCATCACCTT CACCCTCTCC ACTGACAGAA AATTTGTGCC CATTAACATC ACCATCTAAT 841 TCAACAAGAA TTGGGACAAC TCCAGTGAAA AGTTCTTCTC CTTTACCCAT GGGTTTATCG 901 TCATCATCCT TATAATCCAA TATGATGATG GTGGTGAATG CATAGGATCC AGGCCTGTCG 961 ACGCTAGCTT TAACAAAGCG GACTTATAAG TCAGAAGTGA GATGAGATTG AAATAATTTA 1021 TTCCAACTTA ATTCCAATGG ATGATTGGCA GGGATTCCTC TCCTTATATA TATCCTCATG 1081 GTTCGATACC GTCCATATCC GTTTACTCGC TGCAATGCGA TGCATCGGAT CCTAGCTTGC 1141 TTTGCCTGGA TTTCTCTCCC CG //
Primer: ADH-Forward	RDB13522_A5Ipc.seq
>06182_13522_A5Ip_ADH-Forward_F04_16.ab1 1 TATTCTTTAT TGACACACCT CTACCGGCAG ATCCGCTAGG GATAACAGGG TAATATACTA 61 GTAAGATGAT TCTTTTATCG TCGATAACGA TGAGGATGTA GACGATTATG ATGAGAGTGA 121 TTAGCTTCTA AAATTTGGTG TTTTCAAACG CTATTTACAG TACAATCTGA AAAAATTGGA 181 TGAACATTGT AAATGGTAGG ATACGAGTTC CAATTAATGT ATTTTTTAAG TATTACACTT 241 AGCTTATTAA TTTTATATAT ACATTCTACC TCTGTGGGTT TAAAGACAAC TCGTACACAT 301 ACTCAAATTA TTTATGAACA TTATAATGCA TATTCGTACT GTCTATTAAA CATTTTTTTG 361 ACATTAAAAA TTTCGTTTAC TAACGTAGAA AGCGCTGATA ATTGAGGCTA CTATTTTAAT 421 ATCTAGAAAC AAAGATTCAT TAATTAATTA AGTAAGCTCC AGGATACTTG TATATTTCGT 481 TAAAAAAGCG CAATTTCAAC AATTCCTATG AACATCCATG ATTTCATCAC AAAATCAAAC 541 ATATCAGGAT TTAACGTGAA TTATTTGCTT TCTGTGGATT TTCATCTGAT TTCTGGGTCA 601 TTACGTTACT GTAAACAAGC TTTTAAAAAA AAGGATTTTT TACGCAATTA TTATCGATAT 661 TCCGATCTGT AGTATAAAAA AGTTGGCAAC AGTGTGTGAG TTTCCGGAAA CAGGCATTTT 721 TGCCGTTAGG TCTGGAAACT TTCCCGGAAA CATTCACAAC AAAAACTTCT TCACACATTG 781 GTTGGCCAAG GAAATCTGTC GTTCTTACCA GAGACATATT GGCCTTGAAA TGTGCTCGGC 841 TGAGATTGGA AGTCGTGAAG AACGACGGAT GAACTCAGTT GGACCAGTTG AGGTCCCTTT 901 TAGGACTTTG GACCTGATGA GCAAA //
Primer: ars_R1	RDB13522_A5Ipd.seq
>06182_13522_A5Ip_ars_R1_G04_19.ab1 1 AGGTTTTTGG TTTATACTAA TTTACTTAAG TACCTTTAAC TAAACAAAAT GCCTATATAT 61 ATATTAATTT ACAATGAGTG TCACATAAGT CACTATGTCC GAGTGGTTAA GGAGTTAGAC 121 TCGAATTCGC AAGCTTAGCT ACAAATCCCA CTGGCTATAT GTATGCATTT GTGTTAAAAA 181 AGTTTGTATA GATTATTTAA TCTACTCAGC ATTCTTTCTC TAAATAGGAA TTTGTTACTT 241 AATGGAGAAA AAAATGTTTC GATTTACCTA GTGTATTTGT TTGTATACTC ACGTTTAATT 301 TCAAACATCC ATTCTATCTT GTGTAATTTT TGGCATGGTG AAAAAGATAA TCAGCCTTAT 361 AATCTTTACA AAAGTAAGAA ATTCTGTAAA TAAGCCTTAA TGCCCTTGCT TTAAATTAAA 421 ATGGTTCTTT TTCATGATAA TGTTTGCACT TTGTGAATAT ATTTTAGATA GTTCTGTGAG 481 GTATAATTAA GATGTTTTAG AGACTTATAC AATTTTGTCT TTATAAATTC TTAATTGATT 541 TTACCATCCC AGTTTAACTA TGCTTCGTCG GCATCTCTGC ACATGTCGTG TTTTCTTACC 601 GTATTGTCCT ACCAAGAACC TCTTTTTTGC TTGGATCGAA ATTAAAGGTT TAAAAGCAAA 661 GTTATGGATG CTAGAGTATT TCAAAGCTAT TCAGCTAGAG CTGAGGGGAT GAAAAATCCC 721 ATTGCCAAGG AATTGTTGGC TTTGATGGAA GAAAAGCAAA GCAACTTGTC AGTCGCGGTC 781 GATTTGACGA AGAAATCCGA AATCTTAGAA ATTGGTAGAT AAAATTGGAC CCTATGTCTG 841 TGTTATCAAG ACACATATTG AACGTTGTCG AGGATTTCGA CCAGGATATG GTAGAAAAAA 901 CTGGTGGCCT TAGGTAAAAA GCATCGTTTT CTTATCTTTG AGGATCGCAA ATTCCGCAGG 961 ACATTGGAAA TACCGTCAAG CTACAATATG CATCTGGGTG TGTACAAAAT TGCTTCTTGG 1021 GCTCATATCA CAAATGGCCA TACAGTGCCA GGCCGAGGGT ATTATACAAG GCCTCCAAAG 1081 AAGCTGGGTT TACCTTTGGG GACGTGGTCC TCATGCTTTG GCTGAATGGC CTTCCAAGGC 1141 TCTTTGCTAA CTGTCTACAC AGAGGAATCT TAGAAATGGG TTGGGAGAAT GCATCATCCC 1201 GCGACAATTT TTTG //
Primer: f1ori_R3	RDB13522_A5Ipe.seq
>06182_13522_A5Ip_f1ori_R3_H04_22.ab1 1 GCGGGTGGGG GCTTTCGTAT GCGGTGTGAA ATACCGCACA GATGCGTAAG GAGAAAATAC 61 CGCATCAGGC GCCATTCGCC ATTCAGGCTG CGCAACTGTT GGGAAGGGCG ATCGGTGCGG 121 GCCTCTTCGC TATTACGCCA GCTGGCGAAA GGGGGATGTG CTGCAAGGCG ATTAAGTTGG 181 GTAACGCCAG GGTTTTCCCA GTCACGACGT TGTAAAACGA CGGCCAGTGA ATTCGAGCTC 241 GGGAGCGCTA CCGTGAATGG GCTCAACCAA ACAATGAACC TTTTCTTCTG ATTTTCCTAC 301 CACACCGGAA AGGGAGGCAG AAGGCAAAAG GCCCAAGCTA CCAGGAATGA CAGAAGCCTC 361 ATCTGAAATA ATGTCACCAA ACAAGTTGTC AGTCAAAACA ACACCGTTAA GTGTACGAGG 421 GCTCTTGACC AAAAGCATGG CTGCGGAGTC AATGAGCTGG TTTTTTAAGG TAAGGTGAGG 481 ATATTCCTCC TTAAAAATCT TAGCTACAGT CTTGCGCCAA AGACGAGAAG TTGCCAAAAC 541 ATTAGCTTTG TCGAGTAATG TGACGGGAGC AGGAGGGTTG GAAGTTTCAG CTAAGCGGCC 601 GCGGGCCCAA CCTTCCAATT CATTAAATCG AGTCCTCCAT CAGATGATTT ACAAGGTTTT 661 GCATAGAATC CCTAAGTAAG AGAAGTTGTT AGTGATTTCT TCAAGTTTAC TAAAATTTAC 721 ATACAAATGG ATGATCTCTT CTCCATTGTT TCCTTTCTTC TTGCAAACGC GTTTTACAAA 781 GAGATGACAT GCTATTTTCT GATTATTTTT TTCTATAGTT TTCTATTTTG TACGTCCTTG 841 GGAGTCCGAA ATGTAAAATC GGGGTATGGT TAGTAGCGGC AGCGTTCATT ATGGTATATA 901 AAAGATAAAA AGTAGGGGCA AGCGAGACAG AATGGGATAC AAGGGCATCG TCTATAATTA 961 TAGCTAAAAA TTGTATTTTA ATTTGTATTT TTTTGTAATT TTATTTCTCA TTGTTTTACT 1021 TTAAATGAAA TGCGAAATTA GAGAAAACTT CCAACGGAAA TGTCAAAATA AGCTCAGCAA 1081 AAATAATAAC AATTTTACGA AAGCGATCAG CAACTTATTT CTCCGCTGGT AGATTCCTTT 1141 TCCTCATAAA TCAACAATGT ACCTGCCAAT TGGAGTTTTC CCTCCATAGA G //

Please visit Sequencing and PCR primers for primer information.

References

original	Matsuyama, A., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913.
original	Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162.

2018.12.10

GNP_filter3_RDBDEP_html_181012.pl