Please review the QC test results indicated by icon below as well as clone information before placing your order.

pDUAL-HFG1

S. pombe 5 ter end His6-FLAG-GFP(S65C)-tagged vector with nmt1 promoter

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR

In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.

Ordering

Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word] This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).

提供案内 (日本国内) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献 (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) の引用と、　寄託者への謝辞の表明を必要とする。　利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。　寄託者から要請がある場合、理研ＢＲＣは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word] GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします （Schedule AとSchedule Bを入手[link])。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB13522_A5Ipp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: nmt1Fw Sequence file: RDB13522_A5Ipa.seq
>06182_13522_A5Ip_nmt1Fw_D04_10.ab1     1 AGGAATTTTA TTAAATCAGG AAAAACGTAA CTCTCGGCTA CTGGATGGTT CAGTCACCCA    61 ACGATTACTG GGGAGAGAAA ACAGGGCAAA AGCAAAGCTT AAAGGAATCC GATTGTCATT   121 CGGCAATGTG CAGCGAAACT AAAAACCGGA TAATGGACCT GTTAATCGAA ACATTGAAGA   181 TATATAAAGG AAGAGGAATC CTGGCATATC ATCAATTGAA TAAGTTGAAT TAATTATTTC   241 AATCTCATTC TCACTTTCTG ACTTATAGTC GCTTTGTTAA AGCTAGCGTC GACAGGCCTG   301 GATCCTATGC ATCACCACCA TCATCATATG GATTATAAGG ATGATGACGA TAAACCCATG   361 GGTAAAGGAG AAGAACTTTT CACTGGAGTT GTCCCAATTC TTGTTGAATT AGATGGTGAT   421 GTTAATGGGC ACAAATTTTC TGTCAGTGGA GAGGGTGAAG GTGATGCAAC ATACGGAAAA   481 CTTACCCTTA AATTTATTTG CACTACTGGA AAACTACCTG TTCCGTGGCC AACACTTGTC   541 ACTACTTTCT GTTATGGTGT TCAATGCTTT TCAAGATACC CAGATCACAT GAAACAGCAT   601 GACTTTTTCA AGAGTGCCAT GCCCGAAGGC TATGTACAGG AAAGAACTAT ATTTTTCAAA   661 GATGACGGGA ACTACAAGAC ACGTGCTGAA GTCAAGTTTG AAGGTGATAC CCTTGTTAAT   721 AGAATCGAGT TAAAAGGTAT TGATTTTAAA GAAGATGGAA ACATTCTTGG ACACAAATTG   781 GAATACAACT ATAACTCACA CAATGTATAC ATCATGGCAG ACAAACAAAA GAATGGAATC   841 AAAGTTAACT TCAAAATTAG ACACAACATT GAAGATGGAA GCGTTCAACT AGCAGACCAT   901 TATCAACAAA ATACTCCAAT TGGCGATGGC CCTGTCCTTT TACCAGACAA CCATTACCTG   961 TCCACACAAT CTGCCCTTTC GAAAGATCCC AACGAAAAGA GAGACCACAT GGGTCCTTCT  1021 TGAGTTTGTA ACAGCTGCTG GGATTTACAC ATGGCATGGG ATGAACTATA CAAAGGATAT  1081 CATGAGATCT CGACTCTAGA GGATCCGGCG CGCCAACTTC TAATAGCCGA TTCCTAATGG 1141 ATTTATTGAA TTTTATTATT ACATTAGTTA ATTAAAAAAT TAGAGCGT //
Primer: ADH_rev Sequence file: RDB13522_A5Ipb.seq
>06182_13522_A5Ip_ADHrev_E04_13.ab1     1 TAAAGAAATT CTTCAGGAAA TAAGAATTTT CGTTTTAAAA CCTAAGAGTC ACTTTAAAAT    61 TTGTATACAC TTATTTTTTT TATAACTTAT TTAATAATAA AAATCATAAA TCATAAGAAA   121 TTCGCTTATT TAGAAGTGGC GCGCCGGATC CTCTAGAGTC GAGATCTCAT GATATCTTTG   181 TATAGTTCAT CCATGCCATG TGTAATCCCA GCAGCTGTTA CAAACTCAAG AAGGACCATG   241 TGGTCTCTCT TTTCGTTGGG ATCTTTCGAA AGGGCAGATT GTGTGGACAG GTAATGGTTG   301 TCTGGTAAAA GGACAGGGCC ATCGCCAATT GGAGTATTTT GTTGATAATG GTCTGCTAGT   361 TGAACGCTTC CATCTTCAAT GTTGTGTCTA ATTTTGAAGT TAACTTTGAT TCCATTCTTT   421 TGTTTGTCTG CCATGATGTA TACATTGTGT GAGTTATAGT TGTATTCCAA TTTGTGTCCA   481 AGAATGTTTC CATCTTCTTT AAAATCAATA CCTTTTAACT CGATTCTATT AACAAGGGTA   541 TCACCTTCAA ACTTGACTTC AGCACGTGTC TTGTAGTTCC CGTCATCTTT GAAAAATATA   601 GTTCTTTCCT GTACATAGCC TTCGGGCATG GCACTCTTGA AAAAGTCATG CTGTTTCATG   661 TGATCTGGGT ATCTTGAAAA GCATTGAACA CCATAACAGA AAGTAGTGAC AAGTGTTGGC   721 CACGGAACAG GTAGTTTTCC AGTAGTGCAA ATAAATTTAA GGGTAAGTTT TCCGTATGTT   781 GCATCACCTT CACCCTCTCC ACTGACAGAA AATTTGTGCC CATTAACATC ACCATCTAAT   841 TCAACAAGAA TTGGGACAAC TCCAGTGAAA AGTTCTTCTC CTTTACCCAT GGGTTTATCG   901 TCATCATCCT TATAATCCAA TATGATGATG GTGGTGAATG CATAGGATCC AGGCCTGTCG   961 ACGCTAGCTT TAACAAAGCG GACTTATAAG TCAGAAGTGA GATGAGATTG AAATAATTTA  1021 TTCCAACTTA ATTCCAATGG ATGATTGGCA GGGATTCCTC TCCTTATATA TATCCTCATG  1081 GTTCGATACC GTCCATATCC GTTTACTCGC TGCAATGCGA TGCATCGGAT CCTAGCTTGC 1141 TTTGCCTGGA TTTCTCTCCC CG //
Primer: ADH-Forward Sequence file: RDB13522_A5Ipc.seq
>06182_13522_A5Ip_ADH-Forward_F04_16.ab1     1 TATTCTTTAT TGACACACCT CTACCGGCAG ATCCGCTAGG GATAACAGGG TAATATACTA    61 GTAAGATGAT TCTTTTATCG TCGATAACGA TGAGGATGTA GACGATTATG ATGAGAGTGA   121 TTAGCTTCTA AAATTTGGTG TTTTCAAACG CTATTTACAG TACAATCTGA AAAAATTGGA   181 TGAACATTGT AAATGGTAGG ATACGAGTTC CAATTAATGT ATTTTTTAAG TATTACACTT   241 AGCTTATTAA TTTTATATAT ACATTCTACC TCTGTGGGTT TAAAGACAAC TCGTACACAT   301 ACTCAAATTA TTTATGAACA TTATAATGCA TATTCGTACT GTCTATTAAA CATTTTTTTG   361 ACATTAAAAA TTTCGTTTAC TAACGTAGAA AGCGCTGATA ATTGAGGCTA CTATTTTAAT   421 ATCTAGAAAC AAAGATTCAT TAATTAATTA AGTAAGCTCC AGGATACTTG TATATTTCGT   481 TAAAAAAGCG CAATTTCAAC AATTCCTATG AACATCCATG ATTTCATCAC AAAATCAAAC   541 ATATCAGGAT TTAACGTGAA TTATTTGCTT TCTGTGGATT TTCATCTGAT TTCTGGGTCA   601 TTACGTTACT GTAAACAAGC TTTTAAAAAA AAGGATTTTT TACGCAATTA TTATCGATAT   661 TCCGATCTGT AGTATAAAAA AGTTGGCAAC AGTGTGTGAG TTTCCGGAAA CAGGCATTTT   721 TGCCGTTAGG TCTGGAAACT TTCCCGGAAA CATTCACAAC AAAAACTTCT TCACACATTG   781 GTTGGCCAAG GAAATCTGTC GTTCTTACCA GAGACATATT GGCCTTGAAA TGTGCTCGGC   841 TGAGATTGGA AGTCGTGAAG AACGACGGAT GAACTCAGTT GGACCAGTTG AGGTCCCTTT 901 TAGGACTTTG GACCTGATGA GCAAA //
Primer: ars_R1 Sequence file: RDB13522_A5Ipd.seq
>06182_13522_A5Ip_ars_R1_G04_19.ab1     1 AGGTTTTTGG TTTATACTAA TTTACTTAAG TACCTTTAAC TAAACAAAAT GCCTATATAT    61 ATATTAATTT ACAATGAGTG TCACATAAGT CACTATGTCC GAGTGGTTAA GGAGTTAGAC   121 TCGAATTCGC AAGCTTAGCT ACAAATCCCA CTGGCTATAT GTATGCATTT GTGTTAAAAA   181 AGTTTGTATA GATTATTTAA TCTACTCAGC ATTCTTTCTC TAAATAGGAA TTTGTTACTT   241 AATGGAGAAA AAAATGTTTC GATTTACCTA GTGTATTTGT TTGTATACTC ACGTTTAATT   301 TCAAACATCC ATTCTATCTT GTGTAATTTT TGGCATGGTG AAAAAGATAA TCAGCCTTAT   361 AATCTTTACA AAAGTAAGAA ATTCTGTAAA TAAGCCTTAA TGCCCTTGCT TTAAATTAAA   421 ATGGTTCTTT TTCATGATAA TGTTTGCACT TTGTGAATAT ATTTTAGATA GTTCTGTGAG   481 GTATAATTAA GATGTTTTAG AGACTTATAC AATTTTGTCT TTATAAATTC TTAATTGATT   541 TTACCATCCC AGTTTAACTA TGCTTCGTCG GCATCTCTGC ACATGTCGTG TTTTCTTACC   601 GTATTGTCCT ACCAAGAACC TCTTTTTTGC TTGGATCGAA ATTAAAGGTT TAAAAGCAAA   661 GTTATGGATG CTAGAGTATT TCAAAGCTAT TCAGCTAGAG CTGAGGGGAT GAAAAATCCC   721 ATTGCCAAGG AATTGTTGGC TTTGATGGAA GAAAAGCAAA GCAACTTGTC AGTCGCGGTC   781 GATTTGACGA AGAAATCCGA AATCTTAGAA ATTGGTAGAT AAAATTGGAC CCTATGTCTG   841 TGTTATCAAG ACACATATTG AACGTTGTCG AGGATTTCGA CCAGGATATG GTAGAAAAAA   901 CTGGTGGCCT TAGGTAAAAA GCATCGTTTT CTTATCTTTG AGGATCGCAA ATTCCGCAGG   961 ACATTGGAAA TACCGTCAAG CTACAATATG CATCTGGGTG TGTACAAAAT TGCTTCTTGG  1021 GCTCATATCA CAAATGGCCA TACAGTGCCA GGCCGAGGGT ATTATACAAG GCCTCCAAAG  1081 AAGCTGGGTT TACCTTTGGG GACGTGGTCC TCATGCTTTG GCTGAATGGC CTTCCAAGGC  1141 TCTTTGCTAA CTGTCTACAC AGAGGAATCT TAGAAATGGG TTGGGAGAAT GCATCATCCC 1201 GCGACAATTT TTTG //
Primer: f1ori_R3 Sequence file: RDB13522_A5Ipe.seq
>06182_13522_A5Ip_f1ori_R3_H04_22.ab1     1 GCGGGTGGGG GCTTTCGTAT GCGGTGTGAA ATACCGCACA GATGCGTAAG GAGAAAATAC    61 CGCATCAGGC GCCATTCGCC ATTCAGGCTG CGCAACTGTT GGGAAGGGCG ATCGGTGCGG   121 GCCTCTTCGC TATTACGCCA GCTGGCGAAA GGGGGATGTG CTGCAAGGCG ATTAAGTTGG   181 GTAACGCCAG GGTTTTCCCA GTCACGACGT TGTAAAACGA CGGCCAGTGA ATTCGAGCTC   241 GGGAGCGCTA CCGTGAATGG GCTCAACCAA ACAATGAACC TTTTCTTCTG ATTTTCCTAC   301 CACACCGGAA AGGGAGGCAG AAGGCAAAAG GCCCAAGCTA CCAGGAATGA CAGAAGCCTC   361 ATCTGAAATA ATGTCACCAA ACAAGTTGTC AGTCAAAACA ACACCGTTAA GTGTACGAGG   421 GCTCTTGACC AAAAGCATGG CTGCGGAGTC AATGAGCTGG TTTTTTAAGG TAAGGTGAGG   481 ATATTCCTCC TTAAAAATCT TAGCTACAGT CTTGCGCCAA AGACGAGAAG TTGCCAAAAC   541 ATTAGCTTTG TCGAGTAATG TGACGGGAGC AGGAGGGTTG GAAGTTTCAG CTAAGCGGCC   601 GCGGGCCCAA CCTTCCAATT CATTAAATCG AGTCCTCCAT CAGATGATTT ACAAGGTTTT   661 GCATAGAATC CCTAAGTAAG AGAAGTTGTT AGTGATTTCT TCAAGTTTAC TAAAATTTAC   721 ATACAAATGG ATGATCTCTT CTCCATTGTT TCCTTTCTTC TTGCAAACGC GTTTTACAAA   781 GAGATGACAT GCTATTTTCT GATTATTTTT TTCTATAGTT TTCTATTTTG TACGTCCTTG   841 GGAGTCCGAA ATGTAAAATC GGGGTATGGT TAGTAGCGGC AGCGTTCATT ATGGTATATA   901 AAAGATAAAA AGTAGGGGCA AGCGAGACAG AATGGGATAC AAGGGCATCG TCTATAATTA   961 TAGCTAAAAA TTGTATTTTA ATTTGTATTT TTTTGTAATT TTATTTCTCA TTGTTTTACT  1021 TTAAATGAAA TGCGAAATTA GAGAAAACTT CCAACGGAAA TGTCAAAATA AGCTCAGCAA  1081 AAATAATAAC AATTTTACGA AAGCGATCAG CAACTTATTT CTCCGCTGGT AGATTCCTTT 1141 TCCTCATAAA TCAACAATGT ACCTGCCAAT TGGAGTTTTC CCTCCATAGA G //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.07.13

GNP_filter3_RDBDEP_html_220713.pl