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pDUAL-HFG1

S. pombe 5 ter end His6-FLAG-GFP(S65C)-tagged vector with nmt1 promoter

Catalog number RDB06182
Resource name pDUAL-HFG1
Clone info. S. pombe 5 ter end His6-FLAG-GFP(S65C)-tagged vector with nmt1 promoter.
Comment Multicopy marker, ura4+; Integration marker, leu1+. Gateway incompatible.
Vector backbone pDUAL (Plasmid)
Selectable markers Amp^r
Reference of insert sequence AB364049.1 (DDBJ accession number)
Depositor|Developer Yoshida, Minoru | Matsuyama, Akihisa |
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External Database

            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
            Ordering Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
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            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
            提供案内 (日本国内) [open/close]

            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) の引用と、 寄託者への謝辞の表明を必要とする。 利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。 寄託者から要請がある場合、理研BRCは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
            GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします (Schedule AとSchedule Bを入手[link])。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB06182 pDUAL-HFG1 DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Sequence RDB06182z.seq

            Original reference

            original Matsuyama, A., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913.
            original Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Expression Vector Backbone of Schizosaccharomyces pombe (English text)

            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB13522_A5Ipp1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: nmt1Fw
            Sequence file: RDB13522_A5Ipa.seq check
            >06182_13522_A5Ip_nmt1Fw_D04_10.ab1
                1 AGGAATTTTA TTAAATCAGG AAAAACGTAA CTCTCGGCTA CTGGATGGTT CAGTCACCCA
               61 ACGATTACTG GGGAGAGAAA ACAGGGCAAA AGCAAAGCTT AAAGGAATCC GATTGTCATT
              121 CGGCAATGTG CAGCGAAACT AAAAACCGGA TAATGGACCT GTTAATCGAA ACATTGAAGA
              181 TATATAAAGG AAGAGGAATC CTGGCATATC ATCAATTGAA TAAGTTGAAT TAATTATTTC
              241 AATCTCATTC TCACTTTCTG ACTTATAGTC GCTTTGTTAA AGCTAGCGTC GACAGGCCTG
              301 GATCCTATGC ATCACCACCA TCATCATATG GATTATAAGG ATGATGACGA TAAACCCATG
              361 GGTAAAGGAG AAGAACTTTT CACTGGAGTT GTCCCAATTC TTGTTGAATT AGATGGTGAT
              421 GTTAATGGGC ACAAATTTTC TGTCAGTGGA GAGGGTGAAG GTGATGCAAC ATACGGAAAA
              481 CTTACCCTTA AATTTATTTG CACTACTGGA AAACTACCTG TTCCGTGGCC AACACTTGTC
              541 ACTACTTTCT GTTATGGTGT TCAATGCTTT TCAAGATACC CAGATCACAT GAAACAGCAT
              601 GACTTTTTCA AGAGTGCCAT GCCCGAAGGC TATGTACAGG AAAGAACTAT ATTTTTCAAA
              661 GATGACGGGA ACTACAAGAC ACGTGCTGAA GTCAAGTTTG AAGGTGATAC CCTTGTTAAT
              721 AGAATCGAGT TAAAAGGTAT TGATTTTAAA GAAGATGGAA ACATTCTTGG ACACAAATTG
              781 GAATACAACT ATAACTCACA CAATGTATAC ATCATGGCAG ACAAACAAAA GAATGGAATC
              841 AAAGTTAACT TCAAAATTAG ACACAACATT GAAGATGGAA GCGTTCAACT AGCAGACCAT
              901 TATCAACAAA ATACTCCAAT TGGCGATGGC CCTGTCCTTT TACCAGACAA CCATTACCTG
              961 TCCACACAAT CTGCCCTTTC GAAAGATCCC AACGAAAAGA GAGACCACAT GGGTCCTTCT
             1021 TGAGTTTGTA ACAGCTGCTG GGATTTACAC ATGGCATGGG ATGAACTATA CAAAGGATAT
             1081 CATGAGATCT CGACTCTAGA GGATCCGGCG CGCCAACTTC TAATAGCCGA TTCCTAATGG
            1141 ATTTATTGAA TTTTATTATT ACATTAGTTA ATTAAAAAAT TAGAGCGT
            //
            Primer: ADH_rev
            Sequence file: RDB13522_A5Ipb.seq check
            >06182_13522_A5Ip_ADHrev_E04_13.ab1
                1 TAAAGAAATT CTTCAGGAAA TAAGAATTTT CGTTTTAAAA CCTAAGAGTC ACTTTAAAAT
               61 TTGTATACAC TTATTTTTTT TATAACTTAT TTAATAATAA AAATCATAAA TCATAAGAAA
              121 TTCGCTTATT TAGAAGTGGC GCGCCGGATC CTCTAGAGTC GAGATCTCAT GATATCTTTG
              181 TATAGTTCAT CCATGCCATG TGTAATCCCA GCAGCTGTTA CAAACTCAAG AAGGACCATG
              241 TGGTCTCTCT TTTCGTTGGG ATCTTTCGAA AGGGCAGATT GTGTGGACAG GTAATGGTTG
              301 TCTGGTAAAA GGACAGGGCC ATCGCCAATT GGAGTATTTT GTTGATAATG GTCTGCTAGT
              361 TGAACGCTTC CATCTTCAAT GTTGTGTCTA ATTTTGAAGT TAACTTTGAT TCCATTCTTT
              421 TGTTTGTCTG CCATGATGTA TACATTGTGT GAGTTATAGT TGTATTCCAA TTTGTGTCCA
              481 AGAATGTTTC CATCTTCTTT AAAATCAATA CCTTTTAACT CGATTCTATT AACAAGGGTA
              541 TCACCTTCAA ACTTGACTTC AGCACGTGTC TTGTAGTTCC CGTCATCTTT GAAAAATATA
              601 GTTCTTTCCT GTACATAGCC TTCGGGCATG GCACTCTTGA AAAAGTCATG CTGTTTCATG
              661 TGATCTGGGT ATCTTGAAAA GCATTGAACA CCATAACAGA AAGTAGTGAC AAGTGTTGGC
              721 CACGGAACAG GTAGTTTTCC AGTAGTGCAA ATAAATTTAA GGGTAAGTTT TCCGTATGTT
              781 GCATCACCTT CACCCTCTCC ACTGACAGAA AATTTGTGCC CATTAACATC ACCATCTAAT
              841 TCAACAAGAA TTGGGACAAC TCCAGTGAAA AGTTCTTCTC CTTTACCCAT GGGTTTATCG
              901 TCATCATCCT TATAATCCAA TATGATGATG GTGGTGAATG CATAGGATCC AGGCCTGTCG
              961 ACGCTAGCTT TAACAAAGCG GACTTATAAG TCAGAAGTGA GATGAGATTG AAATAATTTA
             1021 TTCCAACTTA ATTCCAATGG ATGATTGGCA GGGATTCCTC TCCTTATATA TATCCTCATG
             1081 GTTCGATACC GTCCATATCC GTTTACTCGC TGCAATGCGA TGCATCGGAT CCTAGCTTGC
            1141 TTTGCCTGGA TTTCTCTCCC CG
            //
            Primer: ADH-Forward
            Sequence file: RDB13522_A5Ipc.seq check
            >06182_13522_A5Ip_ADH-Forward_F04_16.ab1
                1 TATTCTTTAT TGACACACCT CTACCGGCAG ATCCGCTAGG GATAACAGGG TAATATACTA
               61 GTAAGATGAT TCTTTTATCG TCGATAACGA TGAGGATGTA GACGATTATG ATGAGAGTGA
              121 TTAGCTTCTA AAATTTGGTG TTTTCAAACG CTATTTACAG TACAATCTGA AAAAATTGGA
              181 TGAACATTGT AAATGGTAGG ATACGAGTTC CAATTAATGT ATTTTTTAAG TATTACACTT
              241 AGCTTATTAA TTTTATATAT ACATTCTACC TCTGTGGGTT TAAAGACAAC TCGTACACAT
              301 ACTCAAATTA TTTATGAACA TTATAATGCA TATTCGTACT GTCTATTAAA CATTTTTTTG
              361 ACATTAAAAA TTTCGTTTAC TAACGTAGAA AGCGCTGATA ATTGAGGCTA CTATTTTAAT
              421 ATCTAGAAAC AAAGATTCAT TAATTAATTA AGTAAGCTCC AGGATACTTG TATATTTCGT
              481 TAAAAAAGCG CAATTTCAAC AATTCCTATG AACATCCATG ATTTCATCAC AAAATCAAAC
              541 ATATCAGGAT TTAACGTGAA TTATTTGCTT TCTGTGGATT TTCATCTGAT TTCTGGGTCA
              601 TTACGTTACT GTAAACAAGC TTTTAAAAAA AAGGATTTTT TACGCAATTA TTATCGATAT
              661 TCCGATCTGT AGTATAAAAA AGTTGGCAAC AGTGTGTGAG TTTCCGGAAA CAGGCATTTT
              721 TGCCGTTAGG TCTGGAAACT TTCCCGGAAA CATTCACAAC AAAAACTTCT TCACACATTG
              781 GTTGGCCAAG GAAATCTGTC GTTCTTACCA GAGACATATT GGCCTTGAAA TGTGCTCGGC
              841 TGAGATTGGA AGTCGTGAAG AACGACGGAT GAACTCAGTT GGACCAGTTG AGGTCCCTTT
            901 TAGGACTTTG GACCTGATGA GCAAA
            //
            Primer: ars_R1
            Sequence file: RDB13522_A5Ipd.seq check
            >06182_13522_A5Ip_ars_R1_G04_19.ab1
                1 AGGTTTTTGG TTTATACTAA TTTACTTAAG TACCTTTAAC TAAACAAAAT GCCTATATAT
               61 ATATTAATTT ACAATGAGTG TCACATAAGT CACTATGTCC GAGTGGTTAA GGAGTTAGAC
              121 TCGAATTCGC AAGCTTAGCT ACAAATCCCA CTGGCTATAT GTATGCATTT GTGTTAAAAA
              181 AGTTTGTATA GATTATTTAA TCTACTCAGC ATTCTTTCTC TAAATAGGAA TTTGTTACTT
              241 AATGGAGAAA AAAATGTTTC GATTTACCTA GTGTATTTGT TTGTATACTC ACGTTTAATT
              301 TCAAACATCC ATTCTATCTT GTGTAATTTT TGGCATGGTG AAAAAGATAA TCAGCCTTAT
              361 AATCTTTACA AAAGTAAGAA ATTCTGTAAA TAAGCCTTAA TGCCCTTGCT TTAAATTAAA
              421 ATGGTTCTTT TTCATGATAA TGTTTGCACT TTGTGAATAT ATTTTAGATA GTTCTGTGAG
              481 GTATAATTAA GATGTTTTAG AGACTTATAC AATTTTGTCT TTATAAATTC TTAATTGATT
              541 TTACCATCCC AGTTTAACTA TGCTTCGTCG GCATCTCTGC ACATGTCGTG TTTTCTTACC
              601 GTATTGTCCT ACCAAGAACC TCTTTTTTGC TTGGATCGAA ATTAAAGGTT TAAAAGCAAA
              661 GTTATGGATG CTAGAGTATT TCAAAGCTAT TCAGCTAGAG CTGAGGGGAT GAAAAATCCC
              721 ATTGCCAAGG AATTGTTGGC TTTGATGGAA GAAAAGCAAA GCAACTTGTC AGTCGCGGTC
              781 GATTTGACGA AGAAATCCGA AATCTTAGAA ATTGGTAGAT AAAATTGGAC CCTATGTCTG
              841 TGTTATCAAG ACACATATTG AACGTTGTCG AGGATTTCGA CCAGGATATG GTAGAAAAAA
              901 CTGGTGGCCT TAGGTAAAAA GCATCGTTTT CTTATCTTTG AGGATCGCAA ATTCCGCAGG
              961 ACATTGGAAA TACCGTCAAG CTACAATATG CATCTGGGTG TGTACAAAAT TGCTTCTTGG
             1021 GCTCATATCA CAAATGGCCA TACAGTGCCA GGCCGAGGGT ATTATACAAG GCCTCCAAAG
             1081 AAGCTGGGTT TACCTTTGGG GACGTGGTCC TCATGCTTTG GCTGAATGGC CTTCCAAGGC
             1141 TCTTTGCTAA CTGTCTACAC AGAGGAATCT TAGAAATGGG TTGGGAGAAT GCATCATCCC
            1201 GCGACAATTT TTTG
            //
            Primer: f1ori_R3
            Sequence file: RDB13522_A5Ipe.seq check
            >06182_13522_A5Ip_f1ori_R3_H04_22.ab1
                1 GCGGGTGGGG GCTTTCGTAT GCGGTGTGAA ATACCGCACA GATGCGTAAG GAGAAAATAC
               61 CGCATCAGGC GCCATTCGCC ATTCAGGCTG CGCAACTGTT GGGAAGGGCG ATCGGTGCGG
              121 GCCTCTTCGC TATTACGCCA GCTGGCGAAA GGGGGATGTG CTGCAAGGCG ATTAAGTTGG
              181 GTAACGCCAG GGTTTTCCCA GTCACGACGT TGTAAAACGA CGGCCAGTGA ATTCGAGCTC
              241 GGGAGCGCTA CCGTGAATGG GCTCAACCAA ACAATGAACC TTTTCTTCTG ATTTTCCTAC
              301 CACACCGGAA AGGGAGGCAG AAGGCAAAAG GCCCAAGCTA CCAGGAATGA CAGAAGCCTC
              361 ATCTGAAATA ATGTCACCAA ACAAGTTGTC AGTCAAAACA ACACCGTTAA GTGTACGAGG
              421 GCTCTTGACC AAAAGCATGG CTGCGGAGTC AATGAGCTGG TTTTTTAAGG TAAGGTGAGG
              481 ATATTCCTCC TTAAAAATCT TAGCTACAGT CTTGCGCCAA AGACGAGAAG TTGCCAAAAC
              541 ATTAGCTTTG TCGAGTAATG TGACGGGAGC AGGAGGGTTG GAAGTTTCAG CTAAGCGGCC
              601 GCGGGCCCAA CCTTCCAATT CATTAAATCG AGTCCTCCAT CAGATGATTT ACAAGGTTTT
              661 GCATAGAATC CCTAAGTAAG AGAAGTTGTT AGTGATTTCT TCAAGTTTAC TAAAATTTAC
              721 ATACAAATGG ATGATCTCTT CTCCATTGTT TCCTTTCTTC TTGCAAACGC GTTTTACAAA
              781 GAGATGACAT GCTATTTTCT GATTATTTTT TTCTATAGTT TTCTATTTTG TACGTCCTTG
              841 GGAGTCCGAA ATGTAAAATC GGGGTATGGT TAGTAGCGGC AGCGTTCATT ATGGTATATA
              901 AAAGATAAAA AGTAGGGGCA AGCGAGACAG AATGGGATAC AAGGGCATCG TCTATAATTA
              961 TAGCTAAAAA TTGTATTTTA ATTTGTATTT TTTTGTAATT TTATTTCTCA TTGTTTTACT
             1021 TTAAATGAAA TGCGAAATTA GAGAAAACTT CCAACGGAAA TGTCAAAATA AGCTCAGCAA
             1081 AAATAATAAC AATTTTACGA AAGCGATCAG CAACTTATTT CTCCGCTGGT AGATTCCTTT
            1141 TCCTCATAAA TCAACAATGT ACCTGCCAAT TGGAGTTTTC CCTCCATAGA G
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Matsuyama, A., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913.
            original Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162.

            2022.05.18

            GNP_filter3_RDBDEP_html_220516.pl