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pDUAL-HFF41

S. pombe 5 ter end His6-FLAG2-tagged vector with nmt1 (medium strength) promoter

Catalog number RDB06180
Resource name pDUAL-HFF41
Clone info. S. pombe 5 ter end His6-FLAG2-tagged vector with nmt1 (medium strength) promoter.
Comment Multicopy marker, ura4+; Integration marker, leu1+. Gateway incompatible.
Vector backbone pDUAL (Plasmid)
Selectable markers Amp^r
Reference of insert sequence AB364047.1 (DDBJ accession number)
Depositor|Developer Yoshida, Minoru | Matsuyama, Akihisa |
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External Database

            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
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            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) の引用と、 寄託者への謝辞の表明を必要とする。 利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。 寄託者から要請がある場合、理研BRCは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
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            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

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            RDB06180 pDUAL-HFF41 DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Sequence RDB06180z.seq

            Original reference

            original Matsuyama, A., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913.
            original Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Expression Vector Backbone of Schizosaccharomyces pombe (English text)

            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB14482_A6L7p1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: nmt1Fw
            Sequence file: RDB14482_A6L7a.seq check
            >06180_14482_A6L7_nmt1Fw_A01_01_ABI24.ab1
                1 AGGAATTTAT TAATCAGGAA ACGTAACTCT CGGCTACTGG ATGGTTCAGT CACCCAACGA
               61 TTACTGGGGA GAGAAAACAG GGCAAAAGCA AAGCTTAAAG GAATCCGATT GTCATTCGGC
              121 AATGTGCAGC GAAACTAAAA ACCGGATAAT GGACCTGTTA ATCGAAACAT TGAAGATAAA
              181 GGAAGAGGAA TCCTGGCATA TCATCAATTG AATAAGTTGA ATTAATTATT TCAATCTCAT
              241 TCTCACTTTC TGACTTATAG TCGCTTTGTT AAAGCTAGCG TCGACAGGCC TGGATCCTAT
              301 GCATCACCAC CATCATCATA TGGATTATAA GGATGATGAC GATAAACCCA TGGACTACAA
              361 GGACGACGAT GACAAAGATA TCATGAGATC TCGACTCTAG AGGATCCGGC GCGCCACTTC
              421 TAAATAAGCG AATTTCTTAT GATTTATGAT TTTTATTATT AAATAAGTTA TAAAAAAAAT
              481 AAGTGTATAC AAATTTTAAA GTGACTCTTA GGTTTTAAAA CGAAAATTCT TATTCTTGAG
              541 TAACTCTTTC CTGTAGGTCA GGTTGCTTTC TCAGGTATAG TATGAGGTCG CTCTTATTGA
              601 CCACACCTCT ACCGGCAGAT CCGCTAGGGA TAACAGGGTA ATATACTAGT AAGATGATTC
              661 TTTTATCGTC GATAACGATG AGGATGTAGA CGATTATGAT GAGAGTGATT AGCTTCTAAA
              721 ATTTGGTGTT TTCAAACGCT ATTTACAGTA CAATCTGAAA AAATTGGATG AACATTGTAA
              781 ATGGTAGGAT ACGAGTTCCA ATTAATGTAT TTTTTAAGTA TTACACTTAG CTTATTAATT
              841 TTATATATAC ATTCTACCTC TGTGGGTTTA AAGACAACTC GTACACATAC TCAAATTATT
              901 TATGAACATT ATAATGCATA TTCGTACTGT CTATTAAACA TTTTTTTTGA CATTAAAAAT
              961 TTCGTTTACT AACGTAGAAA GCGCTGATAA TTTGAGGCTA CTATTTTAAT ATCTAGAAAC
             1021 AAGATTCATT TAATTTAATT TAAGTAAGCT CCAGGATACT TGTATATTTC GTTAAAAAAA
             1081 GCGCAGTTCA CATCTATGAC ATCCCATGAT CATCACAAAT CCAACCATAT CAAGAATTTA
            1141 AACGTTGAAT TATTTTTGCC TTTTCCTTTG ATGA
            //
            Primer: LEU1pro_F
            Sequence file: RDB14482_A6L7b.seq check
            >06180_14482_A6L7_LEU1pro_F_B01_04_ABI24.ab1
                1 GATAAAGGTG CACAGTGTTG AGTTTCCCGA AACCAGGCAT TTTTGCGGTA GGGTCTGAAA
               61 CTTTCACGAA ACCATTTCAC AACAAAACTT CTTCAACAAC ATGTGTGCAA AGAAAATCGT
              121 CGTCTTACCA GGAGACCATA TTGGCCCTGA AATTGTTGCT TCTGCCTTGG AGGTTTTGAA
              181 AGTCGTTGAG AAGAAGCGAC CTGAGTTAAA ACTCGAGTTT GAAGAACACA AGATTGGAGG
              241 TGCCTCTATT GATGCCTATG GAACCCCTTT GACTGACGAG ACTGTGAAGG CTTGTTTGGA
              301 AGCTGACGGT GTTCTTTTGG GTGCCGTTGG TGGTCCTGAA TGGACCAACC CCAATTGTCG
              361 TCCTGAGCAA GGTTTATTGA AGCTTCGTAA GAGTATGGGT GTTTGGGCCA ACCTTCGACC
              421 TTGCAACTTT GCCAGCAAGT CTTTAGTCAA GTACAGCCCT TTGAAGCCTG AAATCGTTGA
              481 AGGTGTCGAT TTTTGTGTTG TACGAGAACT TACTGGAGGT TGTTACTTTG GTGAGCGCAC
              541 TGAGGACAAC GGATCGGGTT ATGCTATGGA CACTTGGCCT TACAGTTTGG AAGAAGTTTC
              601 TCGTATTGCT CGTTTGGCGG CCGCGGGCCC AACACCAATG TTTATAACCA AGTTTTATCT
              661 TGTTTGTCTA CATGGTATTT TACATTCATC TACATACATC TTTCATTGGC TTTGTACATA
              721 GTTATCATTA CAAGTCTAAA AAAATTCACT CTTTTCTTAT TCAATGTCAA TCCAAGAGAA
              781 AAGATTGTGG TAATGTTGTA GGAGCATGTT TAATAAATTA CTATAGCAAA TTACTTTTTA
              841 TTCCCAAGGT GTTTATCTAT AATAGTTAAT ATTTTAGTCG CTACATAAAA TTTTACCAAA
              901 GAGTACTTGT ATACTAATTC TAAATGCCTT CTGACATAAA ACGCCTAGGA AAACAAACGC
              961 AACAAGGCAT CGACTTTTTC AATAACCAAC CAAAAAATTT TACATTAGTC TTTTTTTTTA
             1021 ATGCTGAGAA AGTCTTTTGC TGATATGCCC TTCCCAACCC AGCTCTCTAT ATCTCTGGCT
             1081 TCGACACAGA TAACGAACCA GCTCCATAGA CCTCCCACGG ACGACCATGG ATGGATGGTC
            1141 GCCTACCCGC AGTTACAATC CACCTCCTTT CCAGGGAGTT ACCGGAAT
            //
            Primer: ars_R1
            Sequence file: RDB14482_A6L7c.seq check
            >06180_14482_A6L7_ars_R1_C01_07_ABI24.ab1
                1 TGGTTCGGTT TATACTAATT ACTTAAGTAC CTTTAACTAA ACAAAATGCC TATATATATA
               61 TTAATTTACA ATGAGTGTCA GATAAGTCAC TATGTCCGAG TGGTTAAGGA GTTAGACTCG
              121 AATTCGCAAG CTTAGCTACA AATCCCACTG GCTATATGTA TGCATTTGTG TTAAAAAAGT
              181 TTGTATAGAT TATTTAATCT ACTCAGCATT CTTTCTCTAA ATAGGAATTT GTTACTTAAT
              241 GGAGAAAAAA ATGTTTCGAT TTACCTAGTG TATTTGTTTG TATACTCACG TTTAATTTCA
              301 AACATCCATT CTATCTTGTG TAATTTTTGG CATGGTGAAA AAGATAATCA GCCTTATAAT
              361 CTTTACAAAA GTAAGAAATT CTGTAAATAA GCCTTAATGC CCTTGCTTTA AATTAAAATG
              421 GTTCTTTTTC ATGATAATGT TTGCACTTTG TGAATATATT TTAGATAGTT CTGTGAGGTA
              481 TAATTAAGAT GTTTTAGAGA CTTATACAAT TTTGTCTTTA TAAATTCTTA ATTGATTTTA
              541 CCATCCCAGT TTAACTATGC TTCGTCGGCA TCTCTGCACA TGTCGTGTTT TCTTACCGTA
              601 TTGTCCTACC AAGAACCTCT TTTTTGCTTG GATCGAAATT AAAGGTTTAA AAGCAAAGTT
              661 ATGGATGCTA GAGTATTTCA AAGCTATTCA GCTAGAGCTG AGGGGATGAA AAATCCCATT
              721 GCCAAGGAAT TGTTGGCTTT GATGGAAGAA AAGCAAAGCA ACTTGTCAGT CGCGGTCGAT
              781 TTGACGAAGA AATCCGAAAT CTTAGAATTG GGTAGATAAA ATTGGACCCT ATGTCTGTGT
              841 TATCAGACAC ATATTGACGT TGTCGAAGGA TTTCGACCAG GATATGGGTA GAAAAACTGG
              901 TGGCTTAGTA AAAAGCATCG TTTTCTTATC TTGAGATCGC AATCGCAGAC ATGGAATACG
              961 GTCAAGCTTA CAATATGCAT CTGGTGTGTA CAAATTGGCT CCTGGGCTCA ATTCAAATTG
            1021 CCATACATGC CAGCAGATTT ATCAGGCGCT AGATGTACCT TGGACTGGCA TCCTTTTGC
            //
            Primer: f1ori_R3
            Sequence file: RDB14482_A6L7d.seq check
            >06180_14482_A6L7_f1ori_R3_D01_10_ABI24.ab1
                1 GCAGTGCTCA TGAGTATGCG GTGTGAATAC CGCACAGATG CGTAAGGAGA AAATACCGCA
               61 TCAGGCGCCA TTCGCCATTC AGGCTGCGCA ACTGTTGGGA AGGGCGATCG GTGCGGGCCT
              121 CTTCGCTATT ACGCCAGCTG GCGAAAGGGG GATGTGCTGC AAGGCGATTA AGTTGGGTAA
              181 CGCCAGGGTT TTCCCAGTCA CGACGTTGTA AAACGACGGC CAGTGAATTC GAGCTCGGGA
              241 GCGCTACCGT GAATGGGCTC AACCAAACAA TGAACCTTTT CTTCTGATTT TCCTACCACA
              301 CCGGAAAGGG AGGCAGAAGG CAAAAGGCCC AAGCTACCAG GAATGACAGA AGCCTCATCT
              361 GAAATAATGT CACCAAACAA GTTGTCAGTC AAAACAACAC CGTTAAGTGT ACGAGGGCTC
              421 TTGACCAAAA GCATGGCTGC GGAGTCAATG AGCTGGTTTT TTAAGGTAAG GTGAGGATAT
              481 TCCTCCTTAA AAATCTTAGC TACAGTCTTG CGCCAAAGAC GAGAAGTTGC CAAAACATTA
              541 GCTTTGTCGA GTAATGTGAC GGGAGCAGGA GGGTTGGAAG TTTCAGCTAA GCGGCCGCGG
              601 GCCCAACCTT CCAATTCATT AAATCGAGTC CTCCATCAGA TGATTTACAA GGTTTTGCAT
              661 AGAATCCCTA AGTAAGAGAA GTTGTTAGTG ATTTCTTCAA GTTTACTAAA ATTTACATAC
              721 AAATGGATGA TCTCTTCTCC ATTGTTTCCT TTCTTCTTGC AAACGCGTTT TACAAAGAGA
              781 TGACATGCTA TTTTCTGATT ATTTTTTTCT ATAGTTTTCT ATTTTGTACG TCCTTGGGAG
              841 TCCGAAATGT AAAATCGGGG TATGGTTAGT AGCGGCAGCG TTCATTATGG TATATAAAAG
              901 ATAAAAAGTA TGGGCAAGCG AGACAGAATG GGATACAAGG GCATCGTCTA TAATTATAGC
              961 TAAAAATTGT ATTTTAATTT GTATTTTTTG TAATTTTATT TCTCATTGTT TTACTTAAAT
             1021 GAATGCGAAT TTAGAGAAAA ACTTTCACCG AATGTCAAAA ATAGCTCAGC AAAATATACC
             1081 ACTTTAGGGA AGCGATCAGC CACTTATCTC GATGTAGATC CGTTTCCTTC ATAAATTCGA
            1141 ACCATTGTTA ACTGTGGCCG AT
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Matsuyama, A., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913.
            original Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162.
            reference Kitamura, K., The type-2 N-end rule peptide recognition activity of Ubr11 ubiquitin ligase is required for the expression of peptide transporters. FEBS Lett. 587 (2): 214-219 (2013). PMID 23219921.
            user_report Kanda, K., Application of a phosphite dehydrogenase gene as a novel dominant selection marker for yeasts. J. Biotechnol. 182-183: 68-73 (2014). PMID 24786825.

            2022.05.18

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