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pDUAL-HFF41 (#RDB06180)

S. pombe 5 ter end His6-FLAG2-tagged vector with nmt1 (medium strength) promoter

Clone info. S. pombe 5 ter end His6-FLAG2-tagged vector with nmt1 (medium strength) promoter.
Comment Multicopy marker, ura4+; Integration marker, leu1+. Gateway incompatible.
Vector backbone pDUAL (RDB06178) (Plasmid)
Selectable markers Amp^r
Reference of insert sequence AB364047.1 (DDBJ accession number)
Depositor|Developer Yoshida, Minoru | Matsuyama, Akihisa |
 
Sequence RDB06180z.seq

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) の引用と、　寄託者への謝辞の表明を必要とする。　利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。　寄託者から要請がある場合、理研ＢＲＣは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。

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RDB06180 pDUAL-HFF41 DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pDUAL-HFF41 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB06180).

Reference section:

Matsuyama, A., Yoshida, M., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913. [link to RRC of NBRP]
Matsuyama, A., Shirai, A., Yashiroda, Y., Kamata, A., Horinouchi, S., Yoshida, M., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB14482_A6L7p1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: nmt1Fw
Sequence file: RDB14482_A6L7a.seq check
>06180_14482_A6L7_nmt1Fw_A01_01_ABI24.ab1
    1 AGGAATTTAT TAATCAGGAA ACGTAACTCT CGGCTACTGG ATGGTTCAGT CACCCAACGA
   61 TTACTGGGGA GAGAAAACAG GGCAAAAGCA AAGCTTAAAG GAATCCGATT GTCATTCGGC
  121 AATGTGCAGC GAAACTAAAA ACCGGATAAT GGACCTGTTA ATCGAAACAT TGAAGATAAA
  181 GGAAGAGGAA TCCTGGCATA TCATCAATTG AATAAGTTGA ATTAATTATT TCAATCTCAT
  241 TCTCACTTTC TGACTTATAG TCGCTTTGTT AAAGCTAGCG TCGACAGGCC TGGATCCTAT
  301 GCATCACCAC CATCATCATA TGGATTATAA GGATGATGAC GATAAACCCA TGGACTACAA
  361 GGACGACGAT GACAAAGATA TCATGAGATC TCGACTCTAG AGGATCCGGC GCGCCACTTC
  421 TAAATAAGCG AATTTCTTAT GATTTATGAT TTTTATTATT AAATAAGTTA TAAAAAAAAT
  481 AAGTGTATAC AAATTTTAAA GTGACTCTTA GGTTTTAAAA CGAAAATTCT TATTCTTGAG
  541 TAACTCTTTC CTGTAGGTCA GGTTGCTTTC TCAGGTATAG TATGAGGTCG CTCTTATTGA
  601 CCACACCTCT ACCGGCAGAT CCGCTAGGGA TAACAGGGTA ATATACTAGT AAGATGATTC
  661 TTTTATCGTC GATAACGATG AGGATGTAGA CGATTATGAT GAGAGTGATT AGCTTCTAAA
  721 ATTTGGTGTT TTCAAACGCT ATTTACAGTA CAATCTGAAA AAATTGGATG AACATTGTAA
  781 ATGGTAGGAT ACGAGTTCCA ATTAATGTAT TTTTTAAGTA TTACACTTAG CTTATTAATT
  841 TTATATATAC ATTCTACCTC TGTGGGTTTA AAGACAACTC GTACACATAC TCAAATTATT
  901 TATGAACATT ATAATGCATA TTCGTACTGT CTATTAAACA TTTTTTTTGA CATTAAAAAT
  961 TTCGTTTACT AACGTAGAAA GCGCTGATAA TTTGAGGCTA CTATTTTAAT ATCTAGAAAC
 1021 AAGATTCATT TAATTTAATT TAAGTAAGCT CCAGGATACT TGTATATTTC GTTAAAAAAA
 1081 GCGCAGTTCA CATCTATGAC ATCCCATGAT CATCACAAAT CCAACCATAT CAAGAATTTA
1141 AACGTTGAAT TATTTTTGCC TTTTCCTTTG ATGA
//
Primer: LEU1pro_F
Sequence file: RDB14482_A6L7b.seq check
>06180_14482_A6L7_LEU1pro_F_B01_04_ABI24.ab1
    1 GATAAAGGTG CACAGTGTTG AGTTTCCCGA AACCAGGCAT TTTTGCGGTA GGGTCTGAAA
   61 CTTTCACGAA ACCATTTCAC AACAAAACTT CTTCAACAAC ATGTGTGCAA AGAAAATCGT
  121 CGTCTTACCA GGAGACCATA TTGGCCCTGA AATTGTTGCT TCTGCCTTGG AGGTTTTGAA
  181 AGTCGTTGAG AAGAAGCGAC CTGAGTTAAA ACTCGAGTTT GAAGAACACA AGATTGGAGG
  241 TGCCTCTATT GATGCCTATG GAACCCCTTT GACTGACGAG ACTGTGAAGG CTTGTTTGGA
  301 AGCTGACGGT GTTCTTTTGG GTGCCGTTGG TGGTCCTGAA TGGACCAACC CCAATTGTCG
  361 TCCTGAGCAA GGTTTATTGA AGCTTCGTAA GAGTATGGGT GTTTGGGCCA ACCTTCGACC
  421 TTGCAACTTT GCCAGCAAGT CTTTAGTCAA GTACAGCCCT TTGAAGCCTG AAATCGTTGA
  481 AGGTGTCGAT TTTTGTGTTG TACGAGAACT TACTGGAGGT TGTTACTTTG GTGAGCGCAC
  541 TGAGGACAAC GGATCGGGTT ATGCTATGGA CACTTGGCCT TACAGTTTGG AAGAAGTTTC
  601 TCGTATTGCT CGTTTGGCGG CCGCGGGCCC AACACCAATG TTTATAACCA AGTTTTATCT
  661 TGTTTGTCTA CATGGTATTT TACATTCATC TACATACATC TTTCATTGGC TTTGTACATA
  721 GTTATCATTA CAAGTCTAAA AAAATTCACT CTTTTCTTAT TCAATGTCAA TCCAAGAGAA
  781 AAGATTGTGG TAATGTTGTA GGAGCATGTT TAATAAATTA CTATAGCAAA TTACTTTTTA
  841 TTCCCAAGGT GTTTATCTAT AATAGTTAAT ATTTTAGTCG CTACATAAAA TTTTACCAAA
  901 GAGTACTTGT ATACTAATTC TAAATGCCTT CTGACATAAA ACGCCTAGGA AAACAAACGC
  961 AACAAGGCAT CGACTTTTTC AATAACCAAC CAAAAAATTT TACATTAGTC TTTTTTTTTA
 1021 ATGCTGAGAA AGTCTTTTGC TGATATGCCC TTCCCAACCC AGCTCTCTAT ATCTCTGGCT
 1081 TCGACACAGA TAACGAACCA GCTCCATAGA CCTCCCACGG ACGACCATGG ATGGATGGTC
1141 GCCTACCCGC AGTTACAATC CACCTCCTTT CCAGGGAGTT ACCGGAAT
//
Primer: ars_R1
Sequence file: RDB14482_A6L7c.seq check
>06180_14482_A6L7_ars_R1_C01_07_ABI24.ab1
    1 TGGTTCGGTT TATACTAATT ACTTAAGTAC CTTTAACTAA ACAAAATGCC TATATATATA
   61 TTAATTTACA ATGAGTGTCA GATAAGTCAC TATGTCCGAG TGGTTAAGGA GTTAGACTCG
  121 AATTCGCAAG CTTAGCTACA AATCCCACTG GCTATATGTA TGCATTTGTG TTAAAAAAGT
  181 TTGTATAGAT TATTTAATCT ACTCAGCATT CTTTCTCTAA ATAGGAATTT GTTACTTAAT
  241 GGAGAAAAAA ATGTTTCGAT TTACCTAGTG TATTTGTTTG TATACTCACG TTTAATTTCA
  301 AACATCCATT CTATCTTGTG TAATTTTTGG CATGGTGAAA AAGATAATCA GCCTTATAAT
  361 CTTTACAAAA GTAAGAAATT CTGTAAATAA GCCTTAATGC CCTTGCTTTA AATTAAAATG
  421 GTTCTTTTTC ATGATAATGT TTGCACTTTG TGAATATATT TTAGATAGTT CTGTGAGGTA
  481 TAATTAAGAT GTTTTAGAGA CTTATACAAT TTTGTCTTTA TAAATTCTTA ATTGATTTTA
  541 CCATCCCAGT TTAACTATGC TTCGTCGGCA TCTCTGCACA TGTCGTGTTT TCTTACCGTA
  601 TTGTCCTACC AAGAACCTCT TTTTTGCTTG GATCGAAATT AAAGGTTTAA AAGCAAAGTT
  661 ATGGATGCTA GAGTATTTCA AAGCTATTCA GCTAGAGCTG AGGGGATGAA AAATCCCATT
  721 GCCAAGGAAT TGTTGGCTTT GATGGAAGAA AAGCAAAGCA ACTTGTCAGT CGCGGTCGAT
  781 TTGACGAAGA AATCCGAAAT CTTAGAATTG GGTAGATAAA ATTGGACCCT ATGTCTGTGT
  841 TATCAGACAC ATATTGACGT TGTCGAAGGA TTTCGACCAG GATATGGGTA GAAAAACTGG
  901 TGGCTTAGTA AAAAGCATCG TTTTCTTATC TTGAGATCGC AATCGCAGAC ATGGAATACG
  961 GTCAAGCTTA CAATATGCAT CTGGTGTGTA CAAATTGGCT CCTGGGCTCA ATTCAAATTG
1021 CCATACATGC CAGCAGATTT ATCAGGCGCT AGATGTACCT TGGACTGGCA TCCTTTTGC
//
Primer: f1ori_R3
Sequence file: RDB14482_A6L7d.seq check
>06180_14482_A6L7_f1ori_R3_D01_10_ABI24.ab1
    1 GCAGTGCTCA TGAGTATGCG GTGTGAATAC CGCACAGATG CGTAAGGAGA AAATACCGCA
   61 TCAGGCGCCA TTCGCCATTC AGGCTGCGCA ACTGTTGGGA AGGGCGATCG GTGCGGGCCT
  121 CTTCGCTATT ACGCCAGCTG GCGAAAGGGG GATGTGCTGC AAGGCGATTA AGTTGGGTAA
  181 CGCCAGGGTT TTCCCAGTCA CGACGTTGTA AAACGACGGC CAGTGAATTC GAGCTCGGGA
  241 GCGCTACCGT GAATGGGCTC AACCAAACAA TGAACCTTTT CTTCTGATTT TCCTACCACA
  301 CCGGAAAGGG AGGCAGAAGG CAAAAGGCCC AAGCTACCAG GAATGACAGA AGCCTCATCT
  361 GAAATAATGT CACCAAACAA GTTGTCAGTC AAAACAACAC CGTTAAGTGT ACGAGGGCTC
  421 TTGACCAAAA GCATGGCTGC GGAGTCAATG AGCTGGTTTT TTAAGGTAAG GTGAGGATAT
  481 TCCTCCTTAA AAATCTTAGC TACAGTCTTG CGCCAAAGAC GAGAAGTTGC CAAAACATTA
  541 GCTTTGTCGA GTAATGTGAC GGGAGCAGGA GGGTTGGAAG TTTCAGCTAA GCGGCCGCGG
  601 GCCCAACCTT CCAATTCATT AAATCGAGTC CTCCATCAGA TGATTTACAA GGTTTTGCAT
  661 AGAATCCCTA AGTAAGAGAA GTTGTTAGTG ATTTCTTCAA GTTTACTAAA ATTTACATAC
  721 AAATGGATGA TCTCTTCTCC ATTGTTTCCT TTCTTCTTGC AAACGCGTTT TACAAAGAGA
  781 TGACATGCTA TTTTCTGATT ATTTTTTTCT ATAGTTTTCT ATTTTGTACG TCCTTGGGAG
  841 TCCGAAATGT AAAATCGGGG TATGGTTAGT AGCGGCAGCG TTCATTATGG TATATAAAAG
  901 ATAAAAAGTA TGGGCAAGCG AGACAGAATG GGATACAAGG GCATCGTCTA TAATTATAGC
  961 TAAAAATTGT ATTTTAATTT GTATTTTTTG TAATTTTATT TCTCATTGTT TTACTTAAAT
 1021 GAATGCGAAT TTAGAGAAAA ACTTTCACCG AATGTCAAAA ATAGCTCAGC AAAATATACC
 1081 ACTTTAGGGA AGCGATCAGC CACTTATCTC GATGTAGATC CGTTTCCTTC ATAAATTCGA
1141 ACCATTGTTA ACTGTGGCCG AT
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Matsuyama, A., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913. [link to RRC of NBRP]
original Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162. [link to RRC of NBRP]
reference Kitamura, K., The type-2 N-end rule peptide recognition activity of Ubr11 ubiquitin ligase is required for the expression of peptide transporters. FEBS Lett. 587 (2): 214-219 (2013). PMID 23219921.
user_report Kanda, K., Application of a phosphite dehydrogenase gene as a novel dominant selection marker for yeasts. J. Biotechnol. 182-183: 68-73 (2014). PMID 24786825. [link to RRC of NBRP]