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pDUAL (#RDB06178)

S. pombe pDUAL series backbone vector

Clone info. S. pombe pDUAL series backbone vector.
Comment Multicopy marker, ura4+; Integration marker, leu1+. Gateway incompatible.
Vector backbone pDUAL (Plasmid)
Selectable markers Amp^r
Reference of insert sequence AB364045.1 (DDBJ accession number)
Depositor|Developer Yoshida, Minoru | Matsuyama, Akihisa |
 
Sequence RDB06178z.seq

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
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手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) の引用と、　寄託者への謝辞の表明を必要とする。　利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。　寄託者から要請がある場合、理研ＢＲＣは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。

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RDB06178 pDUAL DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pDUAL was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB06178).

Reference section:

Matsuyama, A., Yoshida, M., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913. [link to RRC of NBRP]
Matsuyama, A., Shirai, A., Yashiroda, Y., Kamata, A., Horinouchi, S., Yoshida, M., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB13001_A4Js.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: Reverse2
Sequence file: RDB13001_A4Jsa.seq check
>06178_13001_A4Js_Reverse2_A03_03.ab1
    1 ATTCCATGAA TTCGCCAAGC TTGCATGCCT GCAGGTCGAC TCTAGAGGAT CCGGCGCGCC
   61 ACTTCTAAAT AAGCGAATTT CTTATGATTT ATGATTTTTA TTATTAAATA AGTTATAAAA
  121 AAAATAAGTG TATACAAATT TTAAAGTGAC TCTTAGGTTT TAAAACGAAA ATTCTTATTC
  181 TTGAGTAACT CTTTCCTGTA GGTCAGGTTG CTTTCTCAGG TATAGTATGA GGTCGCTCTT
  241 ATTGACCACA CCTCTACCGG CAGATCCGCT AGGGATAACA GGGTAATATA CTAGTAAGAT
  301 GATTCTTTTA TCGTCGATAA CGATGAGGAT GTAGACGATT ATGATGAGAG TGATTAGCTT
  361 CTAAAATTTG GTGTTTTCAA ACGCTATTTA CAGTACAATC TGAAAAAATT GGATGAACAT
  421 TGTAAATGGT AGGATACGAG TTCCAATTAA TGTATTTTTT AAGTATTACA CTTAGCTTAT
  481 TAATTTTATA TATACATTCT ACCTCTGTGG GTTTAAAGAC AACTCGTACA CATACTCAAA
  541 TTATTTATGA ACATTATAAT GCATATTCGT ACTGTCTATT AAACATTTTT TTGACATTAA
  601 AAATTTCGTT TACTAACGTA GAAAGCGCTG ATAATTGAGG CTACTATTTT AATATCTAGA
  661 AACAAAGATT CATTAATTAA TTAAGTAAGC TCCAGGATAC TTGTATATTT CGTTAAAAAA
  721 GCGCAATTTC AACAATTCCT ATGAACATCC ATGATTTCAT CACAAAATCA AACATATCAG
  781 GATTTAACGT GAATTATTTG CTTTCTTGTG ATTTTCATCT GATTTCTGGT CATTTACGTT
  841 ACTGTAAACA AGCTTTTTAA AAAAAAGGAT TTTTTACGCA ATATTATCGA TATCCCAATC
  901 TGTAGTATAA AAAGTGCAAC AGTGTTGAGT TTCCCGAAAC CAGGCATTTT TGCGGTAGGG
  961 TCTGAAACTT TCACGAAACC ATTTCACAAC AAAACTTCTT CAACAACATG TGTGCAAAGA
 1021 AATCGTCGTC TTACCAGGAG ACCATATTGG CCCTGAAATT GTTGCTTCTG CCTTTGGAGG
 1081 TTTTGAAGGT CGGTTGAGAG AGCGACCCTG ACTAAACTCG AGCTTGAGAC ACAGATGAAG
1141 GTGCCCTCCT ATTGAATGCC ATTAGGG
//
Primer: M13_-40
Sequence file: RDB13001_A4Jsb.seq check
>06178_13001_A4Js_M13_-40_B03_06.ab1
    1 AAGGCGGTTA ATCGAGCTCG GGAGCGCTAC CGTGAATGGG CTCAACCAAA CAATGAACCT
   61 TTTCTTCTGA TTTTCCTACC ACACCGGAAA GGGAGGCAGA AGGCAAAAGG CCCAAGCTAC
  121 CAGGAATGAC AGAAGCCTCA TCTGAAATAA TGTCACCAAA CAAGTTGTCA GTCAAAACAA
  181 CACCGTTAAG TGTACGAGGG CTCTTGACCA AAAGCATGGC TGCGGAGTCA ATGAGCTGGT
  241 TTTTTAAGGT AAGGTGAGGA TATTCCTCCT TAAAAATCTT AGCTACAGTC TTGCGCCAAA
  301 GACGAGAAGT TGCCAAAACA TTAGCTTTGT CGAGTAATGT GACGGGAGCA GGAGGGTTGG
  361 AAGTTTCAGC TAAGCGGCCG CGGGCCCAAC CTTCCAATTC ATTAAATCGA GTCCTCCATC
  421 AGATGATTTA CAAGGTTTTG CATAGAATCC CTAAGTAAGA GAAGTTGTTA GTGATTTCTT
  481 CAAGTTTACT AAAATTTACA TACAAATGGA TGATCTCTTC TCCATTGTTT CCTTTCTTCT
  541 TGCAAACGCG TTTTACAAAG AGATGACATG CTATTTTCTG ATTATTTTTT TCTATAGTTT
  601 TCTATTTTGT ACGTCCTTGG GAGTCCGAAA TGTAAAATCG GGGTATGGTT AGTAGCGGCA
  661 GCGTTCATTA TGGTATATAA AAGATAAAAA GTAGGGGCAA GCGAGACAGA ATGGGATACA
  721 AGGGCATCGT CTATAATTAT AGCTAAAAAT TGTATTTTAA TTTGTATTTT TTGTAATTTT
  781 ATTTCTCATT GTTTTACTTA AAATGAATGC GAATTAGAGA AAACTTCAAC GAAATGTCAA
  841 AATAAGCTCA GCAAAATATA CAATTTTAGG GAAAGCGATC AGCAACTTAT TCTCCGTTGT
  901 AGATTCTTTT TCTTCATAAA TTCCAACAAT GTAACTGCCA ATTGATTTTT CTCTAGATGC
  961 TAATAAATTG AAACTAATGA ATATAATAAC ACAGGTTTTG ATAAATTTGT AAACTCTTAC
 1021 TCAAAAAAAG AATGTTAAAA CTAATTTAAC CCTTTTTTAT AAAGGCTCAT GGCGTTTAAA
 1081 GAGGATAGTA AAAAATCTAC ATTTTTTTAA TTAATGTCAC ACTGTCCTGC GATATATTCT
1141 AATAGTTTTT CGCTACTGCC TGTTTTTAGT AACTAATTTA ACCT
//
Primer: ars_R1
Sequence file: RDB13001_A4Jsc.seq check
>06178_13001_A4Js_ars_R1_C03_09.ab1
    1 AAGGGGTCTG TTAATACTAA TTACTTAAGT ACCTTTAACT AAACAAAATG CCTATATATA
   61 TATTAATTTA CAATGAGTGT CAGATAAGTC ACTATGTCCG AGTGGTTAAG GAGTTAGACT
  121 CGAATTCGCA AGCTTAGCTA CAAATCCCAC TGGCTATATG TATGCATTTG TGTTAAAAAA
  181 GTTTGTATAG ATTATTTAAT CTACTCAGCA TTCTTTCTCT AAATAGGAAT TTGTTACTTA
  241 ATGGAGAAAA AAATGTTTCG ATTTACCTAG TGTATTTGTT TGTATACTCA CGTTTAATTT
  301 CAAACATCCA TTCTATCTTG TGTAATTTTT GGCATGGTGA AAAAGATAAT CAGCCTTATA
  361 ATCTTTACAA AAGTAAGAAA TTCTGTAAAT AAGCCTTAAT GCCCTTGCTT TAAATTAAAA
  421 TGGTTCTTTT TCATGATAAT GTTTGCACTT TGTGAATATA TTTTAGATAG TTCTGTGAGG
  481 TATAATTAAG ATGTTTTAGA GACTTATACA ATTTTGTCTT TATAAATTCT TAATTGATTT
  541 TACCATCCCA GTTTAACTAT GCTTCGTCGG CATCTCTGCA CATGTCGTGT TTTCTTACCG
  601 TATTGTCCTA CCAAGAACCT CTTTTTTGCT TGGATCGAAA TTAAAGGTTT AAAAGCAAAG
  661 TTATGGATGC TAGAGTATTT CAAAGCTATT CAGCTAGAGC TGAGGGGATG AAAAATCCCA
  721 TTGCCAAGGA ATTGTTGGCT TTGATGGAAG AAAAGCAAAG CAACTTGTCA GTCGCGGTCG
  781 ATTTGACGAA GAAATCCGAA ATCTTAGAAT TGGTAGATAA AATTGGACCC TATGTCTGTG
  841 TTATCAAGAC ACATATTGAC GTTGTCGAGG ATTTCGACCA GGATATGGTA GAAAAACTGG
  901 TGGCCTTAGG TAAAAAGCAT CGTTTTCTTA TCTTTGAGGA TCGCAAATTC GCAGACATTG
  961 GAAATACCGT CAAGCTACAA TATGCATCTG GTGTGTACAA AATTGCTTCT TGGGCTCATA
 1021 TCACAAATTG CCATACAGTG CCCAGGCGAG GGTATTATAC AGTCCTCAAA AGAAGTTGGG
 1081 TTACCTTTGG GACGTTGATC TCTTGCTTTG GCCTGAAATG TCTCCAAAGG CTCTTTGCTA
1141 CCTGATCTAC CCAGAGAAAA CCTTAGGAAA TGGAGTTTTG AGAAAGCCAA TATGCACG
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Matsuyama, A., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913. [link to RRC of NBRP]
original Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162. [link to RRC of NBRP]
reference Yu, H., Conserved Sequence Preferences Contribute to Substrate Recognition by the Proteasome. J. Biol. Chem. 291 (28): 14526-14539 (2016). PMID 27226608.
reference Huang, S., Identification of Methylosome Components as Negative Regulators of Plant Immunity Using Chemical Genetics. Mol. Plant 9 (12): 1620-1633 (2016). PMID 27756575.
user_report Gu, M., LEM2 recruits CHMP7 for ESCRT-mediated nuclear envelope closure in fission yeast and human cells. Proc. Natl. Acad. Sci. U.S.A. 114 (11): E2166-E2175 (2017). PMID 28242692. [link to RRC of NBRP]
user_report Boehringer J., Structural and functional characterization of Rpn12 identifies residues required for Rpn10 proteasome incorporation. Biochem. J. 448 (1): 55-65 (2012). PMID 22906049. [link to RRC of NBRP]
user_report Wen, J., Splicing-dependent NMD does not require the EJC in Schizosaccharomyces pombe. EMBO J. 29 (9): 1537-1551 (2010). PMID 20360683. [link to RRC of NBRP]
user_report Snaith, H.A., New and old reagents for fluorescent protein tagging of microtubules in fission yeast; experimental and critical evaluation. Methods Cell Biol., 97: 147-172 (2010). PMID 20719270. [link to RRC of NBRP]