RDB06178 - pDUAL

Resource data sheet
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pDUAL

S. pombe pDUAL series backbone vector

Catalog number	RDB06178
Resource name	pDUAL
Clone info.	S. pombe pDUAL series backbone vector.
Comment	Multicopy marker, ura4+; Integration marker, leu1+. Gateway incompatible.
Vector backbone	pDUAL (Plasmid)
Selectable markers	Amp^r
Reference of insert sequence	AB364045.1 (DDBJ accession number)
Depositor\|Developer	Yoshida, Minoru \| Matsuyama, Akihisa \|

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External Database

Reference sequence

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.

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Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

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Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
Ordering	Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. Order form [Credit Card Payment] [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献 (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) の引用と、　寄託者への謝辞の表明を必要とする。　利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。　寄託者から要請がある場合、理研ＢＲＣは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

提供条件

利用者は、研究成果の公表にあたって寄託者の指定する文献 (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) の引用と、　寄託者への謝辞の表明を必要とする。　利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。　寄託者から要請がある場合、理研ＢＲＣは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。

提供依頼

手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
提供依頼書 [Word

]
提供同意書 (MTA、非営利機関による非営利学術研究用)[Word

]

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB06178	pDUAL	DNA solution

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

References and tips

Electronic file

Sequence	RDB06178z.seq

Original reference

original	Matsuyama, A., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913.
original	Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162.

Further references such as user reports and related articles (go to bottom)

Featured content	Expression Vector Backbone of Schizosaccharomyces pombe (English text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB13001_A4Js.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: Reverse2 Sequence file: RDB13001_A4Jsa.seq
>06178_13001_A4Js_Reverse2_A03_03.ab1 1 ATTCCATGAA TTCGCCAAGC TTGCATGCCT GCAGGTCGAC TCTAGAGGAT CCGGCGCGCC 61 ACTTCTAAAT AAGCGAATTT CTTATGATTT ATGATTTTTA TTATTAAATA AGTTATAAAA 121 AAAATAAGTG TATACAAATT TTAAAGTGAC TCTTAGGTTT TAAAACGAAA ATTCTTATTC 181 TTGAGTAACT CTTTCCTGTA GGTCAGGTTG CTTTCTCAGG TATAGTATGA GGTCGCTCTT 241 ATTGACCACA CCTCTACCGG CAGATCCGCT AGGGATAACA GGGTAATATA CTAGTAAGAT 301 GATTCTTTTA TCGTCGATAA CGATGAGGAT GTAGACGATT ATGATGAGAG TGATTAGCTT 361 CTAAAATTTG GTGTTTTCAA ACGCTATTTA CAGTACAATC TGAAAAAATT GGATGAACAT 421 TGTAAATGGT AGGATACGAG TTCCAATTAA TGTATTTTTT AAGTATTACA CTTAGCTTAT 481 TAATTTTATA TATACATTCT ACCTCTGTGG GTTTAAAGAC AACTCGTACA CATACTCAAA 541 TTATTTATGA ACATTATAAT GCATATTCGT ACTGTCTATT AAACATTTTT TTGACATTAA 601 AAATTTCGTT TACTAACGTA GAAAGCGCTG ATAATTGAGG CTACTATTTT AATATCTAGA 661 AACAAAGATT CATTAATTAA TTAAGTAAGC TCCAGGATAC TTGTATATTT CGTTAAAAAA 721 GCGCAATTTC AACAATTCCT ATGAACATCC ATGATTTCAT CACAAAATCA AACATATCAG 781 GATTTAACGT GAATTATTTG CTTTCTTGTG ATTTTCATCT GATTTCTGGT CATTTACGTT 841 ACTGTAAACA AGCTTTTTAA AAAAAAGGAT TTTTTACGCA ATATTATCGA TATCCCAATC 901 TGTAGTATAA AAAGTGCAAC AGTGTTGAGT TTCCCGAAAC CAGGCATTTT TGCGGTAGGG 961 TCTGAAACTT TCACGAAACC ATTTCACAAC AAAACTTCTT CAACAACATG TGTGCAAAGA 1021 AATCGTCGTC TTACCAGGAG ACCATATTGG CCCTGAAATT GTTGCTTCTG CCTTTGGAGG 1081 TTTTGAAGGT CGGTTGAGAG AGCGACCCTG ACTAAACTCG AGCTTGAGAC ACAGATGAAG 1141 GTGCCCTCCT ATTGAATGCC ATTAGGG //
Primer: M13_-40 Sequence file: RDB13001_A4Jsb.seq
>06178_13001_A4Js_M13_-40_B03_06.ab1 1 AAGGCGGTTA ATCGAGCTCG GGAGCGCTAC CGTGAATGGG CTCAACCAAA CAATGAACCT 61 TTTCTTCTGA TTTTCCTACC ACACCGGAAA GGGAGGCAGA AGGCAAAAGG CCCAAGCTAC 121 CAGGAATGAC AGAAGCCTCA TCTGAAATAA TGTCACCAAA CAAGTTGTCA GTCAAAACAA 181 CACCGTTAAG TGTACGAGGG CTCTTGACCA AAAGCATGGC TGCGGAGTCA ATGAGCTGGT 241 TTTTTAAGGT AAGGTGAGGA TATTCCTCCT TAAAAATCTT AGCTACAGTC TTGCGCCAAA 301 GACGAGAAGT TGCCAAAACA TTAGCTTTGT CGAGTAATGT GACGGGAGCA GGAGGGTTGG 361 AAGTTTCAGC TAAGCGGCCG CGGGCCCAAC CTTCCAATTC ATTAAATCGA GTCCTCCATC 421 AGATGATTTA CAAGGTTTTG CATAGAATCC CTAAGTAAGA GAAGTTGTTA GTGATTTCTT 481 CAAGTTTACT AAAATTTACA TACAAATGGA TGATCTCTTC TCCATTGTTT CCTTTCTTCT 541 TGCAAACGCG TTTTACAAAG AGATGACATG CTATTTTCTG ATTATTTTTT TCTATAGTTT 601 TCTATTTTGT ACGTCCTTGG GAGTCCGAAA TGTAAAATCG GGGTATGGTT AGTAGCGGCA 661 GCGTTCATTA TGGTATATAA AAGATAAAAA GTAGGGGCAA GCGAGACAGA ATGGGATACA 721 AGGGCATCGT CTATAATTAT AGCTAAAAAT TGTATTTTAA TTTGTATTTT TTGTAATTTT 781 ATTTCTCATT GTTTTACTTA AAATGAATGC GAATTAGAGA AAACTTCAAC GAAATGTCAA 841 AATAAGCTCA GCAAAATATA CAATTTTAGG GAAAGCGATC AGCAACTTAT TCTCCGTTGT 901 AGATTCTTTT TCTTCATAAA TTCCAACAAT GTAACTGCCA ATTGATTTTT CTCTAGATGC 961 TAATAAATTG AAACTAATGA ATATAATAAC ACAGGTTTTG ATAAATTTGT AAACTCTTAC 1021 TCAAAAAAAG AATGTTAAAA CTAATTTAAC CCTTTTTTAT AAAGGCTCAT GGCGTTTAAA 1081 GAGGATAGTA AAAAATCTAC ATTTTTTTAA TTAATGTCAC ACTGTCCTGC GATATATTCT 1141 AATAGTTTTT CGCTACTGCC TGTTTTTAGT AACTAATTTA ACCT //
Primer: ars_R1 Sequence file: RDB13001_A4Jsc.seq
>06178_13001_A4Js_ars_R1_C03_09.ab1 1 AAGGGGTCTG TTAATACTAA TTACTTAAGT ACCTTTAACT AAACAAAATG CCTATATATA 61 TATTAATTTA CAATGAGTGT CAGATAAGTC ACTATGTCCG AGTGGTTAAG GAGTTAGACT 121 CGAATTCGCA AGCTTAGCTA CAAATCCCAC TGGCTATATG TATGCATTTG TGTTAAAAAA 181 GTTTGTATAG ATTATTTAAT CTACTCAGCA TTCTTTCTCT AAATAGGAAT TTGTTACTTA 241 ATGGAGAAAA AAATGTTTCG ATTTACCTAG TGTATTTGTT TGTATACTCA CGTTTAATTT 301 CAAACATCCA TTCTATCTTG TGTAATTTTT GGCATGGTGA AAAAGATAAT CAGCCTTATA 361 ATCTTTACAA AAGTAAGAAA TTCTGTAAAT AAGCCTTAAT GCCCTTGCTT TAAATTAAAA 421 TGGTTCTTTT TCATGATAAT GTTTGCACTT TGTGAATATA TTTTAGATAG TTCTGTGAGG 481 TATAATTAAG ATGTTTTAGA GACTTATACA ATTTTGTCTT TATAAATTCT TAATTGATTT 541 TACCATCCCA GTTTAACTAT GCTTCGTCGG CATCTCTGCA CATGTCGTGT TTTCTTACCG 601 TATTGTCCTA CCAAGAACCT CTTTTTTGCT TGGATCGAAA TTAAAGGTTT AAAAGCAAAG 661 TTATGGATGC TAGAGTATTT CAAAGCTATT CAGCTAGAGC TGAGGGGATG AAAAATCCCA 721 TTGCCAAGGA ATTGTTGGCT TTGATGGAAG AAAAGCAAAG CAACTTGTCA GTCGCGGTCG 781 ATTTGACGAA GAAATCCGAA ATCTTAGAAT TGGTAGATAA AATTGGACCC TATGTCTGTG 841 TTATCAAGAC ACATATTGAC GTTGTCGAGG ATTTCGACCA GGATATGGTA GAAAAACTGG 901 TGGCCTTAGG TAAAAAGCAT CGTTTTCTTA TCTTTGAGGA TCGCAAATTC GCAGACATTG 961 GAAATACCGT CAAGCTACAA TATGCATCTG GTGTGTACAA AATTGCTTCT TGGGCTCATA 1021 TCACAAATTG CCATACAGTG CCCAGGCGAG GGTATTATAC AGTCCTCAAA AGAAGTTGGG 1081 TTACCTTTGG GACGTTGATC TCTTGCTTTG GCCTGAAATG TCTCCAAAGG CTCTTTGCTA 1141 CCTGATCTAC CCAGAGAAAA CCTTAGGAAA TGGAGTTTTG AGAAAGCCAA TATGCACG //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Matsuyama, A., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913.
original	Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162.
reference	Yu, H., Conserved Sequence Preferences Contribute to Substrate Recognition by the Proteasome. J. Biol. Chem. 291 (28): 14526-14539 (2016). PMID 27226608.
reference	Huang, S., Identification of Methylosome Components as Negative Regulators of Plant Immunity Using Chemical Genetics. Mol. Plant 9 (12): 1620-1633 (2016). PMID 27756575.
user_report	Gu, M., LEM2 recruits CHMP7 for ESCRT-mediated nuclear envelope closure in fission yeast and human cells. Proc. Natl. Acad. Sci. U.S.A. 114 (11): E2166-E2175 (2017). PMID 28242692.
user_report	Boehringer J., Structural and functional characterization of Rpn12 identifies residues required for Rpn10 proteasome incorporation. Biochem. J. 448 (1): 55-65 (2012). PMID 22906049.
user_report	Wen, J., Splicing-dependent NMD does not require the EJC in Schizosaccharomyces pombe. EMBO J. 29 (9): 1537-1551 (2010). PMID 20360683.
user_report	Snaith, H.A., New and old reagents for fluorescent protein tagging of microtubules in fission yeast; experimental and critical evaluation. Methods Cell Biol., 97: 147-172 (2010). PMID 20719270.

2022.05.18

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