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pDUAL2-GFH1c

S. pombe 3 ter end GFP(S65C)-FLAG-His6-tagged vector with nmt1 promoter

Catalog number RDB06169
Resource name pDUAL2-GFH1c
Clone info. S. pombe 3 ter end GFP(S65C)-FLAG-His6-tagged vector with nmt1 promoter. Multicopy marker, ura4+; Integration marker, ura4+, leu1-.
Comment Gateway compatible.
Vector backbone pDUAL2 (Plasmid)
Selectable markers E. coli: Amp^r, Cm^r, ccdB.
Growth remarks Use DB3.1, ccdB Survival or equivalent to amplify this clone. 30 degC is fine.
Reference of insert sequence AB364092.1 (DDBJ accession number)
Depositor|Developer Yoshida, Minoru | Matsuyama, Akihisa |
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External Database

            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
            Ordering Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
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            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
            Remarks Use Survival2 (Invitrogen) or equivalent to amplify this clone.
            提供案内 (日本国内) [open/close]

            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) の引用と、 寄託者への謝辞の表明を必要とする。 利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。 寄託者から要請がある場合、理研BRCは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
            GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします (Schedule AとSchedule Bを入手[link])。
            備考 このクローンの増殖にはSurvival2 (Invitrogen) あるいは同等の宿主を使用してください。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB06169 pDUAL2-GFH1c DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Sequence RDB06169z.seq

            Original reference

            original Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Expression Vector Backbone of Schizosaccharomyces pombe (English text)

            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB12792_A4Eq.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: ADHrev
            Sequence file: RDB12792_A4Eqa.seq check
            >06169_12792_A4Eq_nmt1_Fw_F10_16.ab1
                1 AGGGAAATTT ATTTAAATCA AGGAAAACGT AACTCTCGGC TACTGGATGG TTCAGTCACC
               61 CAACGATTAC TGGGGCAGAG AAAACAGGGC AAAAGCAAAG CTTAAAGGAA TCCGATTGTC
              121 ATTCGGCAAT GTGCAGCGAA ACTAAAAACC GGATAATGGA CCTGTTAATC GAAACATTGA
              181 AGATATATAA AGGAAGAGGA ATCCTGGCAT ATCATCAATT GAATAAGTTG AATTAATTAT
              241 TTCAATCTCA TTCTCACTTT CTGACTTATA GTCGCTTTGT TAAAGCTAGC GTCGACAGGC
              301 CTGGATCCGG ATATCAACAA GTTTGTACAA AAAAGCTGAA CGAGAAACGT AAAATGATAT
              361 AAATATCAAT ATATTAAATT AGATTTTGCA TAAAAAACAG ACTACATAAT ACTGTAAAAC
              421 ACAACATATC CAGTCACTAT GGCGGCCGCA TTAGGCACCC CAGGCTTTAC ACTTTATGCT
              481 TCCGGCTCGT ATAATGTGTG GATTTTGAGT TAGGATCCGT CGAGATTTTC AGGAGCTAAG
              541 GAAGCTAAAA TGGAGAAAAA AATCACTGGA TATACCACCG TTGATATATC CCAATGGCAT
              601 CGTAAAGAAC ATTTTGAGGC ATTTCAGTCA GTTGCTCAAT GTACCTATAA CCAGACCGTT
              661 CAGCTGGATA TTACGGCCTT TTTAAAGACC GTAAAGAAAA ATAAGCACAA GTTTTATCCG
              721 GCCTTTATTC ACATTCTTGC CCGCCTGATG AATGCTCATC CGGAATTCCG TATGGCAATG
              781 AAAGACGGTG AGCTGGTGAT ATGGGATAGT GTTCACCCTT GTTACACCGT TTTCCATGAG
              841 CAAACTGAAA CGTTTTCATC GCTCTGGAGT GAATACCACG ACGATTTCCG GCAGTTTCTA
              901 CACATATATT CGCAAGATGT GGCGTGTTAC GGTGAAAACC TGGGCCTATT TCCCTAAAGG
              961 GTTTATTGAG AATATGTTTT TCGTCTCAGC CAATCCCTGG GTGAGTTTCA CCAGTTTTGA
             1021 TTTAAACGTG GCCAATATGG ACAACTTCTT CGCCCCCGTT TTCACATGGG CAAATATTTA
             1081 TACGCAAGGC GACCAAGGTG CTGATGCGCT GGCGATTCAG GTCATTCATG CCGATTGTGA
            1141 ATGCTTCATG TTCGGCCAGA ATGCCTTAAT TGAACTTACA CCAACACGAG
            //
            Primer: nmt1Fw
            Sequence file: RDB12792_A4Eqb.seq check
            >06169_12792_A4Eq_ADHrev_E10_13.ab1
                1 GGAGAGTTCT CAAGATAAGA ATTTTCGTTT TAAAACCTAA GAGTCACTTT AAAATTTGTA
               61 TACACTTATT TTTTTTATAA CTTATTTAAT AATAAAAATC ATAAATCATA AGAAATTCGC
              121 TTATTTAGAA GTGGCGCGCC GGATCCTCTA GAGTCGAGAT CTCAGTGGTG ATGGTGGTGA
              181 TGTTTGTCAT CGTCGTCCTT GTAGTCCATG GGTTTGTATA GTTCATCCAT GCCATGTGTA
              241 ATCCCAGCAG CTGTTACAAA CTCAAGAAGG ACCATGTGGT CTCTCTTTTC GTTGGGATCT
              301 TTCGAAAGGG CAGATTGTGT GGACAGGTAA TGGTTGTCTG GTAAAAGGAC AGGGCCATCG
              361 CCAATTGGAG TATTTTGTTG ATAATGGTCT GCTAGTTGAA CGCTTCCATC TTCAATGTTG
              421 TGTCTAATTT TGAAGTTAAC TTTGATTCCA TTCTTTTGTT TGTCTGCCAT GATGTATACA
              481 TTGTGTGAGT TATAGTTGTA TTCCAATTTG TGTCCAAGAA TGTTTCCATC TTCTTTAAAA
              541 TCAATACCTT TTAACTCGAT TCTATTAACA AGGGTATCAC CTTCAAACTT GACTTCAGCA
              601 CGTGTCTTGT AGTTCCCGTC ATCTTTGAAA AATATAGTTC TTTCCTGTAC ATAGCCTTCG
              661 GGCATGGCAC TCTTGAAAAA GTCATGCTGT TTCATGTGAT CTGGGTATCT TGAAAAGCAT
              721 TGAACACCAT AACAGAAAGT AGTGACAAGT GTTGGCCACG GAACAGGTAG TTTTCCAGTA
              781 GTGCAAATAA ATTTAAGGGT AAGTTTTCCG TATGTTGCAT CACCTTCACC CTCTCCACTG
              841 ACAGAAAATT TGTGCCCATT AACATCACCA TCTAATTCAA CAAGAATTGG GACAACTCCA
              901 GTGAAAAGTT CTTCTCCTTT ACTCATGATA TCAACCACTT TGTACAGAAA GCCTGAAACG
              961 AGAAACGTAA AATGATATAA ATATCAATAT ATTAAATTAG ATTTTGCATA AAAAACAGAC
             1021 TACATAATAC TGTAAAACAC AACATATCCC AGTCACTATG GTCGACCTGC AGACTGGCCT
             1081 GTGTTATAAG GCAGCCCTGA CATTTATATT CCCAGACCAT TCAGTAATGG GCGATTTTTG
            1141 ATTGTTCAAT TTTTTCGCCG GGGTGAGC
            //
            Primer: Reverse2
            Sequence file: RDB12792_A4Eqc.seq check
            >06169_12792_A4Eq_Reverse2_G10_19.ab1
                1 AATAATGATT ACGCCAGCTT GTGATATTGA CGAAACTTTT TGACATCTAA TTTATTCTGT
               61 TCCAACACCA ATGTTTATAA CCAAGTTTTA TCTTGTTTGT CTACATGGTA TTTTACATTC
              121 ATCTACATAC ATCTTTCATT GGCTTTGTAC ATAGTTATCA TTACAAGTCT AAAAAAATTC
              181 ACTCTTTTCT TATTCAATGT CAATCCAAGA GAAAAGATTG TGGTAATGTT GTAGGAGCAT
              241 GTTTAATAAA TTACTATAGC AAATTACTTT TTATTCCCAA GGTGTTTATC TATAATAGTT
              301 AATATTTTAG TCGCTACATA AAATTTTACC AAAGAGTACT TGTATACTAA TTCTAAATGC
              361 CTTCTGACAT AAAACGCCTA GGAAAACAAA CGCAAACAAG GCATCGACTT TTTCAATAAC
              421 CAACCAAAAA AATTTTACAT TAGTCTTTTT TTAATGCTGA GAAAGTCTTT GCTGATATGC
              481 CTTCCAACCA GCTTCTCTAT ATCTCTTGGC TTCGACAACA GGATTACGAC CAGCTCCATA
              541 GACTCCACGA CCAACAATGA TGATGTCGCT ACCGCAGTTT ACAATCACTT CTTCAGGAGT
              601 ACGATATTGC TGTCCCAGCC CGTCTCCTTT AACATCCAAG CCGATACCAG GGGACATAGT
              661 TATGTAGTCG CTTTGAAGGT TAGGAAATCG ACGACCAGCT ATAAAGCCAA AGCAAAAATC
              721 GGTATGCTTC TCAAACCATT CTAAGGTTTT CTCTGTGTAG GAACCAGTAG CCAAAGAGCC
              781 TTTGGAAGAC ATTTCAGCCA AAAGCAAGAG ACCACGTCCC AAAGGTAAAC CAACTTCTTT
              841 GAGGCCTTGT ATAATACCCT CGCCTGGCAC TGTATGGCAA TTTGTGATAT GAGCCCAAGA
              901 AGCAATTTTG TACACACCAG ATGCATATTG TAGCTTGACG GTATTTCCAA TGTCTGCGAA
              961 TTTGCGATCC TCAAAGATAA GAAAACGATG CTTTTTACCT AAGGCCACCA GTTTTTCTAC
             1021 CATATCCCTG GTCGAAATCC TCGACAACGT CAATATGTGT CTTGATACAC AGACATAGGT
             1081 TCAAATTTAT TCTACAATTC TAGATTTCGA TTCTCGTTCA AATTCGAACC GCGAACTGAC
            1141 CAAGTTTGCC TTTTGCCTTT TCTTTCCAAT TCCA
            //
            Primer: Cm_C_F
            Sequence file: RDB12792_A4Eqd.seq check
            >06169_12792_A4Eq_Cm_C_F_H10_22.ab1
                1 GGGCTGACTG GATCGGCTTA CTAAAAGCCA GATAACAGTA TGCGTATTTG CGCGCTGATT
               61 TTTGCGGTAT AAGAATATAT ACTGATATGT ATACCCGAAG TATGTCAAAA AGAGGTATGC
              121 TATGAAGCAG CGTATTACAG TGACAGTTGA CAGCGACAGC TATCAGTTGC TCAAGGCATA
              181 TATGATGTCA ATATCTCCGG TCTGGTAAGC ACAACCATGC AGAATGAAGC CCGTCGTCTG
              241 CGTGCCGAAC GCTGGAAAGC GGAAAATCAG GAAGGGATGG CTGAGGTCGC CCGGTTTATT
              301 GAAATGAACG GCTCTTTTGC TGACGAGAAC AGGGGCTGGT GAAATGCAGT TTAAGGTTTA
              361 CACCTATAAA AGAGAGAGCC GTTATCGTCT GTTTGTGGAT GTACAGAGTG ATATTATTGA
              421 CACGCCCGGG CGACGGATGG TGATCCCCCT GGCCAGTGCA CGTCTGCTGT CAGATAAAGT
              481 CTCCCGTGAA CTTTACCCGG TGGTGCATAT CGGGGATGAA AGCTGGCGCA TGATGACCAC
              541 CGATATGGCC AGTGTGCCGG TCTCCGTTAT CGGGGAAGAA GTGGCTGATC TCAGCCACCG
              601 CGAAAATGAC ATCAAAAACG CCATTAACCT GATGTTCTGG GGAATATAAA TGTCAGGCTC
              661 CCTTATACAC AGCCAGTCTG CAGGTCGACC ATAGTGACTG GATATGTTGT GTTTTACAGT
              721 ATTATGTAGT CTGTTTTTTA TGCAAAATCT AATTTAATAT ATTGATATTT ATATCATTTT
              781 ACGTTTCTCG TTCAGCTTTC TTGTACAAAG TGGTTGATAT CATGAGTAAA GGAGAAGAAC
              841 TTTTCACTGG AGTTGTCCCA ATTCTTGTTG AATTAGATGG TGATGTTAAT GGGCACAAAT
              901 TTTCTGTCAG TGGAGAGGGT GAAAGGTGAT GCAACATACG GAAAACTTAC CCTTAAATTT
              961 ATTTGCACTA CTGGAAAACT ACCTGTTCCG TGGCCAACAC TTTGTCACTA CTTTCTGTTA
             1021 TGGTGTCATG CTTTTTCAGA TACCCAGATC ACATGGAAAC AGCATGACTT TTTCAGAGTG
             1081 CCATGGCGGA TGCTATGTAC AGAAGACTAT ATTTTCAGAT GACTGACTAC AGACACGTGC
            1141 CTGAGCTCCA GTTTGAAAGG GTGA
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162.

            2022.07.13

            GNP_filter3_RDBDEP_html_220713.pl