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pDUAL2-GFH1c

S. pombe 3 ter end GFP(S65C)-FLAG-His6-tagged vector with nmt1 promoter

Catalog number RDB06169
Resource name pDUAL2-GFH1c
Clone info. S. pombe 3 ter end GFP(S65C)-FLAG-His6-tagged vector with nmt1 promoter. Multicopy marker, ura4+; Integration marker, ura4+, leu1-.
Gateway compatible.
Vector backbone pDUAL2 (Plasmid)
Selectable markers E. coli: Amp^r, Cm^r, ccdB.
Growth remarks Use DB3.1, ccdB Survival or equivalent to amplify this clone. 30 degC is fine.
Reference of nucleotide sequence AB364092.1 (DDBJ accession number)
Depositor Yoshida, Minoru | Matsuyama, Akihisa |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
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Material Transfer Agreement (MTA) [Word]
Schedule A (GFP-A) [Link]
Remarks Use Survival2 (Invitrogen) or equivalent to amplify this clone.
Please use Schedule A (GFP-A) additionally.
Distribution information (domestic users) [open/close]


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RDB06169 pDUAL2-GFH1c DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB06169z.seq from Depositor

Original reference

original Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Expression Vector Backbone of Schizosaccharomyces pombe (English text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB12792_A4Eq.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: ADHrev | Sequence: RDB12792_A4Eqa.seq
>06169_12792_A4Eq_nmt1_Fw_F10_16.ab1
    1 AGGGAAATTT ATTTAAATCA AGGAAAACGT AACTCTCGGC TACTGGATGG TTCAGTCACC
   61 CAACGATTAC TGGGGCAGAG AAAACAGGGC AAAAGCAAAG CTTAAAGGAA TCCGATTGTC
  121 ATTCGGCAAT GTGCAGCGAA ACTAAAAACC GGATAATGGA CCTGTTAATC GAAACATTGA
  181 AGATATATAA AGGAAGAGGA ATCCTGGCAT ATCATCAATT GAATAAGTTG AATTAATTAT
  241 TTCAATCTCA TTCTCACTTT CTGACTTATA GTCGCTTTGT TAAAGCTAGC GTCGACAGGC
  301 CTGGATCCGG ATATCAACAA GTTTGTACAA AAAAGCTGAA CGAGAAACGT AAAATGATAT
  361 AAATATCAAT ATATTAAATT AGATTTTGCA TAAAAAACAG ACTACATAAT ACTGTAAAAC
  421 ACAACATATC CAGTCACTAT GGCGGCCGCA TTAGGCACCC CAGGCTTTAC ACTTTATGCT
  481 TCCGGCTCGT ATAATGTGTG GATTTTGAGT TAGGATCCGT CGAGATTTTC AGGAGCTAAG
  541 GAAGCTAAAA TGGAGAAAAA AATCACTGGA TATACCACCG TTGATATATC CCAATGGCAT
  601 CGTAAAGAAC ATTTTGAGGC ATTTCAGTCA GTTGCTCAAT GTACCTATAA CCAGACCGTT
  661 CAGCTGGATA TTACGGCCTT TTTAAAGACC GTAAAGAAAA ATAAGCACAA GTTTTATCCG
  721 GCCTTTATTC ACATTCTTGC CCGCCTGATG AATGCTCATC CGGAATTCCG TATGGCAATG
  781 AAAGACGGTG AGCTGGTGAT ATGGGATAGT GTTCACCCTT GTTACACCGT TTTCCATGAG
  841 CAAACTGAAA CGTTTTCATC GCTCTGGAGT GAATACCACG ACGATTTCCG GCAGTTTCTA
  901 CACATATATT CGCAAGATGT GGCGTGTTAC GGTGAAAACC TGGGCCTATT TCCCTAAAGG
  961 GTTTATTGAG AATATGTTTT TCGTCTCAGC CAATCCCTGG GTGAGTTTCA CCAGTTTTGA
 1021 TTTAAACGTG GCCAATATGG ACAACTTCTT CGCCCCCGTT TTCACATGGG CAAATATTTA
 1081 TACGCAAGGC GACCAAGGTG CTGATGCGCT GGCGATTCAG GTCATTCATG CCGATTGTGA
1141 ATGCTTCATG TTCGGCCAGA ATGCCTTAAT TGAACTTACA CCAACACGAG
//
Primer: nmt1Fw | Sequence: RDB12792_A4Eqb.seq
>06169_12792_A4Eq_ADHrev_E10_13.ab1
    1 GGAGAGTTCT CAAGATAAGA ATTTTCGTTT TAAAACCTAA GAGTCACTTT AAAATTTGTA
   61 TACACTTATT TTTTTTATAA CTTATTTAAT AATAAAAATC ATAAATCATA AGAAATTCGC
  121 TTATTTAGAA GTGGCGCGCC GGATCCTCTA GAGTCGAGAT CTCAGTGGTG ATGGTGGTGA
  181 TGTTTGTCAT CGTCGTCCTT GTAGTCCATG GGTTTGTATA GTTCATCCAT GCCATGTGTA
  241 ATCCCAGCAG CTGTTACAAA CTCAAGAAGG ACCATGTGGT CTCTCTTTTC GTTGGGATCT
  301 TTCGAAAGGG CAGATTGTGT GGACAGGTAA TGGTTGTCTG GTAAAAGGAC AGGGCCATCG
  361 CCAATTGGAG TATTTTGTTG ATAATGGTCT GCTAGTTGAA CGCTTCCATC TTCAATGTTG
  421 TGTCTAATTT TGAAGTTAAC TTTGATTCCA TTCTTTTGTT TGTCTGCCAT GATGTATACA
  481 TTGTGTGAGT TATAGTTGTA TTCCAATTTG TGTCCAAGAA TGTTTCCATC TTCTTTAAAA
  541 TCAATACCTT TTAACTCGAT TCTATTAACA AGGGTATCAC CTTCAAACTT GACTTCAGCA
  601 CGTGTCTTGT AGTTCCCGTC ATCTTTGAAA AATATAGTTC TTTCCTGTAC ATAGCCTTCG
  661 GGCATGGCAC TCTTGAAAAA GTCATGCTGT TTCATGTGAT CTGGGTATCT TGAAAAGCAT
  721 TGAACACCAT AACAGAAAGT AGTGACAAGT GTTGGCCACG GAACAGGTAG TTTTCCAGTA
  781 GTGCAAATAA ATTTAAGGGT AAGTTTTCCG TATGTTGCAT CACCTTCACC CTCTCCACTG
  841 ACAGAAAATT TGTGCCCATT AACATCACCA TCTAATTCAA CAAGAATTGG GACAACTCCA
  901 GTGAAAAGTT CTTCTCCTTT ACTCATGATA TCAACCACTT TGTACAGAAA GCCTGAAACG
  961 AGAAACGTAA AATGATATAA ATATCAATAT ATTAAATTAG ATTTTGCATA AAAAACAGAC
 1021 TACATAATAC TGTAAAACAC AACATATCCC AGTCACTATG GTCGACCTGC AGACTGGCCT
 1081 GTGTTATAAG GCAGCCCTGA CATTTATATT CCCAGACCAT TCAGTAATGG GCGATTTTTG
1141 ATTGTTCAAT TTTTTCGCCG GGGTGAGC
//
Primer: Reverse2 | Sequence: RDB12792_A4Eqc.seq
>06169_12792_A4Eq_Reverse2_G10_19.ab1
    1 AATAATGATT ACGCCAGCTT GTGATATTGA CGAAACTTTT TGACATCTAA TTTATTCTGT
   61 TCCAACACCA ATGTTTATAA CCAAGTTTTA TCTTGTTTGT CTACATGGTA TTTTACATTC
  121 ATCTACATAC ATCTTTCATT GGCTTTGTAC ATAGTTATCA TTACAAGTCT AAAAAAATTC
  181 ACTCTTTTCT TATTCAATGT CAATCCAAGA GAAAAGATTG TGGTAATGTT GTAGGAGCAT
  241 GTTTAATAAA TTACTATAGC AAATTACTTT TTATTCCCAA GGTGTTTATC TATAATAGTT
  301 AATATTTTAG TCGCTACATA AAATTTTACC AAAGAGTACT TGTATACTAA TTCTAAATGC
  361 CTTCTGACAT AAAACGCCTA GGAAAACAAA CGCAAACAAG GCATCGACTT TTTCAATAAC
  421 CAACCAAAAA AATTTTACAT TAGTCTTTTT TTAATGCTGA GAAAGTCTTT GCTGATATGC
  481 CTTCCAACCA GCTTCTCTAT ATCTCTTGGC TTCGACAACA GGATTACGAC CAGCTCCATA
  541 GACTCCACGA CCAACAATGA TGATGTCGCT ACCGCAGTTT ACAATCACTT CTTCAGGAGT
  601 ACGATATTGC TGTCCCAGCC CGTCTCCTTT AACATCCAAG CCGATACCAG GGGACATAGT
  661 TATGTAGTCG CTTTGAAGGT TAGGAAATCG ACGACCAGCT ATAAAGCCAA AGCAAAAATC
  721 GGTATGCTTC TCAAACCATT CTAAGGTTTT CTCTGTGTAG GAACCAGTAG CCAAAGAGCC
  781 TTTGGAAGAC ATTTCAGCCA AAAGCAAGAG ACCACGTCCC AAAGGTAAAC CAACTTCTTT
  841 GAGGCCTTGT ATAATACCCT CGCCTGGCAC TGTATGGCAA TTTGTGATAT GAGCCCAAGA
  901 AGCAATTTTG TACACACCAG ATGCATATTG TAGCTTGACG GTATTTCCAA TGTCTGCGAA
  961 TTTGCGATCC TCAAAGATAA GAAAACGATG CTTTTTACCT AAGGCCACCA GTTTTTCTAC
 1021 CATATCCCTG GTCGAAATCC TCGACAACGT CAATATGTGT CTTGATACAC AGACATAGGT
 1081 TCAAATTTAT TCTACAATTC TAGATTTCGA TTCTCGTTCA AATTCGAACC GCGAACTGAC
1141 CAAGTTTGCC TTTTGCCTTT TCTTTCCAAT TCCA
//
Primer: Cm_C_F | Sequence: RDB12792_A4Eqd.seq
>06169_12792_A4Eq_Cm_C_F_H10_22.ab1
    1 GGGCTGACTG GATCGGCTTA CTAAAAGCCA GATAACAGTA TGCGTATTTG CGCGCTGATT
   61 TTTGCGGTAT AAGAATATAT ACTGATATGT ATACCCGAAG TATGTCAAAA AGAGGTATGC
  121 TATGAAGCAG CGTATTACAG TGACAGTTGA CAGCGACAGC TATCAGTTGC TCAAGGCATA
  181 TATGATGTCA ATATCTCCGG TCTGGTAAGC ACAACCATGC AGAATGAAGC CCGTCGTCTG
  241 CGTGCCGAAC GCTGGAAAGC GGAAAATCAG GAAGGGATGG CTGAGGTCGC CCGGTTTATT
  301 GAAATGAACG GCTCTTTTGC TGACGAGAAC AGGGGCTGGT GAAATGCAGT TTAAGGTTTA
  361 CACCTATAAA AGAGAGAGCC GTTATCGTCT GTTTGTGGAT GTACAGAGTG ATATTATTGA
  421 CACGCCCGGG CGACGGATGG TGATCCCCCT GGCCAGTGCA CGTCTGCTGT CAGATAAAGT
  481 CTCCCGTGAA CTTTACCCGG TGGTGCATAT CGGGGATGAA AGCTGGCGCA TGATGACCAC
  541 CGATATGGCC AGTGTGCCGG TCTCCGTTAT CGGGGAAGAA GTGGCTGATC TCAGCCACCG
  601 CGAAAATGAC ATCAAAAACG CCATTAACCT GATGTTCTGG GGAATATAAA TGTCAGGCTC
  661 CCTTATACAC AGCCAGTCTG CAGGTCGACC ATAGTGACTG GATATGTTGT GTTTTACAGT
  721 ATTATGTAGT CTGTTTTTTA TGCAAAATCT AATTTAATAT ATTGATATTT ATATCATTTT
  781 ACGTTTCTCG TTCAGCTTTC TTGTACAAAG TGGTTGATAT CATGAGTAAA GGAGAAGAAC
  841 TTTTCACTGG AGTTGTCCCA ATTCTTGTTG AATTAGATGG TGATGTTAAT GGGCACAAAT
  901 TTTCTGTCAG TGGAGAGGGT GAAAGGTGAT GCAACATACG GAAAACTTAC CCTTAAATTT
  961 ATTTGCACTA CTGGAAAACT ACCTGTTCCG TGGCCAACAC TTTGTCACTA CTTTCTGTTA
 1021 TGGTGTCATG CTTTTTCAGA TACCCAGATC ACATGGAAAC AGCATGACTT TTTCAGAGTG
 1081 CCATGGCGGA TGCTATGTAC AGAAGACTAT ATTTTCAGAT GACTGACTAC AGACACGTGC
1141 CTGAGCTCCA GTTTGAAAGG GTGA
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162.

2019.09.27

GNP_filter3_RDBDEP_html_190927.pl