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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pDUAL2-FFH1c (#RDB06166)

S. pombe 3 ter end FLAG2-His6-tagged vector with nmt1 promoter

Clone info. S. pombe 3 ter end FLAG2-His6-tagged vector with nmt1 promoter. Multicopy marker, ura4+; Integration marker, ura4+, leu1-.
Comment Gateway compatible.
Vector backbone pDUAL2 (RDB06192) (Plasmid)
Selectable markers E. coli: Amp^r, Cm^r, ccdB.
Growth remarks Use DB3.1, ccdB Survival or equivalent to amplify this clone. 30 degC is fine.
Reference of insert sequence AB364089.1 (DDBJ accession number)
Depositor|Developer Yoshida, Minoru | Matsuyama, Akihisa |
 
Sequence RDB06166z.seq

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
Remarks Use Survival2 (Invitrogen) or equivalent to amplify this clone.
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) の引用と、　寄託者への謝辞の表明を必要とする。　利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。　寄託者から要請がある場合、理研ＢＲＣは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。
備考 このクローンの増殖にはSurvival2 (Invitrogen) あるいは同等の宿主を使用してください。

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RDB06166 pDUAL2-FFH1c DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pDUAL2-FFH1c was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB06166).

Reference section:

Matsuyama, A., Shirai, A., Yashiroda, Y., Kamata, A., Horinouchi, S., Yoshida, M., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB13299_A5Df.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: nmt1Fw
Sequence file: RDB13299_A5Dfa.seq check
>06166_13299_A5Df_nmt1Fw_G02_20.ab1
    1 TATTTATTTA ATCAGGAAAC GTAACTCTCG GCTACTGGAT GGTTCAGTCA CCCAACGATT
   61 ACTGGGGAGA GAAAACAGGG CAAAAGCAAA GCTTAAAGGA ATCCGATTGT CATTCGGCAA
  121 TGTGCAGCGA AACTAAAAAC CGGATAATGG ACCTGTTAAT CGAAACATTG AAGATATATA
  181 AAGGAAGAGG AATCCTGGCA TATCATCAAT TGAATAAGTT GAATTAATTA TTTCAATCTC
  241 ATTCTCACTT TCTGACTTAT AGTCGCTTTG TTAAAGCTAG CGTCGACAGG CCTGGATCCG
  301 GATATCAACA AGTTTGTACA AAAAAGCTGA ACGAGAAACG TAAAATGATA TAAATATCAA
  361 TATATTAAAT TAGATTTTGC ATAAAAAACA GACTACATAA TACTGTAAAA CACAACATAT
  421 CCAGTCACTA TGGCGGCCGC ATTAGGCACC CCAGGCTTTA CACTTTATGC TTCCGGCTCG
  481 TATAATGTGT GGATTTTGAG TTAGGATCCG TCGAGATTTT CAGGAGCTAA GGAAGCTAAA
  541 ATGGAGAAAA AAATCACTGG ATATACCACC GTTGATATAT CCCAATGGCA TCGTAAAGAA
  601 CATTTTGAGG CATTTCAGTC AGTTGCTCAA TGTACCTATA ACCAGACCGT TCAGCTGGAT
  661 ATTACGGCCT TTTTAAAGAC CGTAAAGAAA AATAAGCACA AGTTTTATCC GGCCTTTATT
  721 CACATTCTTG CCCGCCTGAT GAATGCTCAT CCGGAATTCC GTATGGCAAT GAAAGACGGT
  781 GAGCTGGTGA TATGGGATAG TGTTCACCCT TGTTACACCG TTTTCCATGA GCAAACTGAA
  841 ACGTTTTCAT CGCTCTGGAG TGAATACCAC GACGATTTCC GGCAGTTTCT ACACATATAT
  901 TCGCAAGATG TGGCGTGTTA CGGTGAAAAC CTGGCCTATT TCCCTAAAGG GTTTATTGAG
  961 AATATGTTTT TCGTCTCAGC CAATCCCTGG GTGAGTTTCA CCAGTTTTGA TTTAAACGTG
 1021 GCCATATGGG ACACTTCTTC GCCCCGTTTT CACATGGCAA ATATATACGC AGCCGACAGT
 1081 GCTGATGGCG CTGCGAATCA GGTTCATCAT GGCGTTGTGA TGGCCTTCCA ATGTCGGCGA
1141 GAATGCTTAA TGGCAT
//
Primer: ADH_rev
Sequence file: RDB13299_A5Dfb.seq check
>06166_13299_A5Df_ADH_rev_H02_23.ab1
    1 TGGAAGGTTA TCAAGATAAG AATTTTCGTT TTAAACCTAA GGAGTCACTT TAAAATTTGT
   61 ATACACTTAT TTTTTTTATA ACTTATTTAA TAATAAAAAT CATAAATCAT AAGAAATTCG
  121 CTTATTTAGA AGTGGCGCGC CGGATCCTCT AGAGTCGAGA TCTCAGTGGT GATGGTGGTG
  181 ATGTTTGTCA TCGTCGTCCT TGTAGTCCAT GGGTTTATCG TCATCATCCT TATAATCCAT
  241 GATATCAACC ACTTTGTACA AGAAAGCTGA ACGAGAAACG TAAAATGATA TAAATATCAA
  301 TATATTAAAT TAGATTTTGC ATAAAAAACA GACTACATAA TACTGTAAAA CACAACATAT
  361 CCAGTCACTA TGGTCGACCT GCAGACTGGC TGTGTATAAG GGAGCCTGAC ATTTATATTC
  421 CCCAGAACAT CAGGTTAATG GCGTTTTTGA TGTCATTTTC GCGGTGGCTG AGATCAGCCA
  481 CTTCTTCCCC GATAACGGAG ACCGGCACAC TGGCCATATC GGTGGTCATC ATGCGCCAGC
  541 TTTCATCCCC GATATGCACC ACCGGGTAAA GTTCACGGGA GACTTTATCT GACAGCAGAC
  601 GTGCACTGGC CAGGGGGATC ACCATCCGTC GCCCGGGCGT GTCAATAATA TCACTCTGTA
  661 CATCCACAAA CAGACGATAA CGGCTCTCTC TTTTATAGGT GTAAACCTTA AACTGCATTT
  721 CACCAGCCCC TGTTCTCGTC AGCAAAAGAG CCGTTCATTT CAATAAACCG GGCGACCTCA
  781 GCCATCCCTT CCTGATTTTC CGCTTTCCAG CGTTCGGCAC GCAGACGACG GGCTTCATTC
  841 TGCATGGTTG TGCTTACCAG ACCGGAGATA TTGACATCAT ATATGCCTTG AGCAACTGAT
  901 AGCTGTCGCT GTCAACTGTC ACTGTAATAC GCTGCTTCAT AGCATACCTC TTTTTGACAT
  961 ACTTCGGGTA TACATATCAG TATATATTCT TATACCCGCA AAAATCAGCG CGCAAATACG
 1021 CATACTGTTA TCTGGGCTTT TAGTAAGCCG GATCAGATCT TACGCCCCGC CTGGCACTCA
 1081 TCGCAGTACT GTTGTAATTC ATTAAGCATC TGCCGACATG CAAGGCAATC ACAACCGGCA
1141 ATGGAAATTG GAACT
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162. [link to RRC of NBRP]