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pDUAL2-FFH1c

S. pombe 3 ter end FLAG2-His6-tagged vector with nmt1 promoter

Catalog number RDB06166
Resource name pDUAL2-FFH1c
Clone info. S. pombe 3 ter end FLAG2-His6-tagged vector with nmt1 promoter. Multicopy marker, ura4+; Integration marker, ura4+, leu1-.
Gateway compatible.
Vector backbone pDUAL2 (Plasmid)
Selectable markers E. coli: Amp^r, Cm^r, ccdB.
Growth remarks Use DB3.1, ccdB Survival or equivalent to amplify this clone. 30 degC is fine.
Reference of nucleotide sequence AB364089.1 (DDBJ accession number)
Depositor Yoshida, Minoru | Matsuyama, Akihisa |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
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Material Transfer Agreement (MTA) [Word]
Remarks Use Survival2 (Invitrogen) or equivalent to amplify this clone.
Distribution information (domestic users) [open/close]


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RDB06166 pDUAL2-FFH1c DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB06166z.seq from Depositor

Original reference

original Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Expression Vector Backbone of Schizosaccharomyces pombe (English text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB13299_A5Df.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: nmt1Fw | Sequence: RDB13299_A5Dfa.seq
>06166_13299_A5Df_nmt1Fw_G02_20.ab1
    1 TATTTATTTA ATCAGGAAAC GTAACTCTCG GCTACTGGAT GGTTCAGTCA CCCAACGATT
   61 ACTGGGGAGA GAAAACAGGG CAAAAGCAAA GCTTAAAGGA ATCCGATTGT CATTCGGCAA
  121 TGTGCAGCGA AACTAAAAAC CGGATAATGG ACCTGTTAAT CGAAACATTG AAGATATATA
  181 AAGGAAGAGG AATCCTGGCA TATCATCAAT TGAATAAGTT GAATTAATTA TTTCAATCTC
  241 ATTCTCACTT TCTGACTTAT AGTCGCTTTG TTAAAGCTAG CGTCGACAGG CCTGGATCCG
  301 GATATCAACA AGTTTGTACA AAAAAGCTGA ACGAGAAACG TAAAATGATA TAAATATCAA
  361 TATATTAAAT TAGATTTTGC ATAAAAAACA GACTACATAA TACTGTAAAA CACAACATAT
  421 CCAGTCACTA TGGCGGCCGC ATTAGGCACC CCAGGCTTTA CACTTTATGC TTCCGGCTCG
  481 TATAATGTGT GGATTTTGAG TTAGGATCCG TCGAGATTTT CAGGAGCTAA GGAAGCTAAA
  541 ATGGAGAAAA AAATCACTGG ATATACCACC GTTGATATAT CCCAATGGCA TCGTAAAGAA
  601 CATTTTGAGG CATTTCAGTC AGTTGCTCAA TGTACCTATA ACCAGACCGT TCAGCTGGAT
  661 ATTACGGCCT TTTTAAAGAC CGTAAAGAAA AATAAGCACA AGTTTTATCC GGCCTTTATT
  721 CACATTCTTG CCCGCCTGAT GAATGCTCAT CCGGAATTCC GTATGGCAAT GAAAGACGGT
  781 GAGCTGGTGA TATGGGATAG TGTTCACCCT TGTTACACCG TTTTCCATGA GCAAACTGAA
  841 ACGTTTTCAT CGCTCTGGAG TGAATACCAC GACGATTTCC GGCAGTTTCT ACACATATAT
  901 TCGCAAGATG TGGCGTGTTA CGGTGAAAAC CTGGCCTATT TCCCTAAAGG GTTTATTGAG
  961 AATATGTTTT TCGTCTCAGC CAATCCCTGG GTGAGTTTCA CCAGTTTTGA TTTAAACGTG
 1021 GCCATATGGG ACACTTCTTC GCCCCGTTTT CACATGGCAA ATATATACGC AGCCGACAGT
 1081 GCTGATGGCG CTGCGAATCA GGTTCATCAT GGCGTTGTGA TGGCCTTCCA ATGTCGGCGA
1141 GAATGCTTAA TGGCAT
//
Primer: ADH_rev | Sequence: RDB13299_A5Dfb.seq
>06166_13299_A5Df_ADH_rev_H02_23.ab1
    1 TGGAAGGTTA TCAAGATAAG AATTTTCGTT TTAAACCTAA GGAGTCACTT TAAAATTTGT
   61 ATACACTTAT TTTTTTTATA ACTTATTTAA TAATAAAAAT CATAAATCAT AAGAAATTCG
  121 CTTATTTAGA AGTGGCGCGC CGGATCCTCT AGAGTCGAGA TCTCAGTGGT GATGGTGGTG
  181 ATGTTTGTCA TCGTCGTCCT TGTAGTCCAT GGGTTTATCG TCATCATCCT TATAATCCAT
  241 GATATCAACC ACTTTGTACA AGAAAGCTGA ACGAGAAACG TAAAATGATA TAAATATCAA
  301 TATATTAAAT TAGATTTTGC ATAAAAAACA GACTACATAA TACTGTAAAA CACAACATAT
  361 CCAGTCACTA TGGTCGACCT GCAGACTGGC TGTGTATAAG GGAGCCTGAC ATTTATATTC
  421 CCCAGAACAT CAGGTTAATG GCGTTTTTGA TGTCATTTTC GCGGTGGCTG AGATCAGCCA
  481 CTTCTTCCCC GATAACGGAG ACCGGCACAC TGGCCATATC GGTGGTCATC ATGCGCCAGC
  541 TTTCATCCCC GATATGCACC ACCGGGTAAA GTTCACGGGA GACTTTATCT GACAGCAGAC
  601 GTGCACTGGC CAGGGGGATC ACCATCCGTC GCCCGGGCGT GTCAATAATA TCACTCTGTA
  661 CATCCACAAA CAGACGATAA CGGCTCTCTC TTTTATAGGT GTAAACCTTA AACTGCATTT
  721 CACCAGCCCC TGTTCTCGTC AGCAAAAGAG CCGTTCATTT CAATAAACCG GGCGACCTCA
  781 GCCATCCCTT CCTGATTTTC CGCTTTCCAG CGTTCGGCAC GCAGACGACG GGCTTCATTC
  841 TGCATGGTTG TGCTTACCAG ACCGGAGATA TTGACATCAT ATATGCCTTG AGCAACTGAT
  901 AGCTGTCGCT GTCAACTGTC ACTGTAATAC GCTGCTTCAT AGCATACCTC TTTTTGACAT
  961 ACTTCGGGTA TACATATCAG TATATATTCT TATACCCGCA AAAATCAGCG CGCAAATACG
 1021 CATACTGTTA TCTGGGCTTT TAGTAAGCCG GATCAGATCT TACGCCCCGC CTGGCACTCA
 1081 TCGCAGTACT GTTGTAATTC ATTAAGCATC TGCCGACATG CAAGGCAATC ACAACCGGCA
1141 ATGGAAATTG GAACT
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162.

2019.09.27

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