Resource data sheet
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S. pombe 5 ter end His6-FLAG-CFP-tagged vector with nmt1 promoter

Catalog number RDB06160
Resource name pDUAL2-HFC1c
Clone info. S. pombe 5 ter end His6-FLAG-CFP-tagged vector with nmt1 promoter. Multicopy marker, ura4+; Integration marker, ura4+, leu1-.
Comment Gateway compatible.
Vector backbone pDUAL2 (Plasmid)
Selectable markers E. coli: Amp^r, Cm^r, ccdB.
Growth remarks Use DB3.1, ccdB Survival or equivalent to amplify this clone. 30 degC is fine.
Reference of insert sequence AB364107.1 (DDBJ accession number)
Depositor|Developer Yoshida, Minoru | Matsuyama, Akihisa |
Sequence (full) RDB06160hts01.seqcheck assembled from experimentally sequenced data
Test sheet RDB20263_B4Cjp1-1.pdf 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature(s). In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR . When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
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MTA, for use for not-for-profit academic purpose [Word]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Remarks Use Survival2 (Invitrogen) or equivalent to amplify this clone.
提供案内 (日本国内) [open/close]

提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献の引用と、寄託者への謝辞の表明を必要とする。 利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。 寄託者から要請がある場合、理研BRCは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。
提供依頼書 [Word]
提供同意書 (MTA, 非営利学術目的用)[Word]
備考 このクローンの増殖にはSurvival2 (Invitrogen) あるいは同等の宿主を使用してください。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB06160 pDUAL2-HFC1c DNA solution

How to cite this biological resource

Materials & Methods section:

The pDUAL2-HFC1c was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB06160).

Reference section:

Further references such as user reports and related articles (go to bottom)


Original, user report and related articles

user_report Jakociunas, T., Two portable recombination enhancers direct donor choice in fission yeast heterochromatin. PLoS Genet., 9 (10): e1003762 (2013). PMID 24204285. [link to RRC of NBRP]