Please review the QC test results indicated by icon below as well as clone information before placing your order.

pDUAL2-HFG1c

S. pombe 5 ter end His6-FLAG-GFP(S65C)-tagged vector with nmt1 promoter

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
Ordering	Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word] This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
Remarks	Use Survival2 (Invitrogen) or equivalent to amplify this clone.

提供案内 (日本国内) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献 (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) の引用と、　寄託者への謝辞の表明を必要とする。　利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。　寄託者から要請がある場合、理研ＢＲＣは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word] GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします （Schedule AとSchedule Bを入手[link])。
備考	このクローンの増殖にはSurvival2 (Invitrogen) あるいは同等の宿主を使用してください。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB12791_A4Eq.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: ADHrev Sequence file: RDB12791_A4Eqa.seq
>06157_12791_A4Eq_nmt1_Fw_B10_04.ab1     1 ATGGATATTT TATTTAAATC AGGAAAAACG TAACTCTCGG CTACTGGATG GTTCAGTCAC    61 CCAACGATTA CTGGGCGAGA GAAAACAGGG CAAAAGCAAA GCTTAAAGGA ATCCGATTGT   121 CATTCGGCAA TGTGCAGCGA AACTAAAAAC CGGATAATGG ACCTGTTAAT CGAAACATTG   181 AAGATATATA AAGGAAGAGG AATCCTGGCA TATCATCAAT TGAATAAGTT GAATTAATTA   241 TTTCAATCTC ATTCTCACTT TCTGACTTAT AGTCGCTTTG TTAAAGCTAG CGTCGACAGG   301 CCTGGATCCT ATGCATCACC ACCATCATCA TATGGATTAT AAGGATGATG ACGATAAACC   361 CATGGGTAAA GGAGAAGAAC TTTTCACTGG AGTTGTCCCA ATTCTTGTTG AATTAGATGG   421 TGATGTTAAT GGGCACAAAT TTTCTGTCAG TGGAGAGGGT GAAGGTGATG CAACATACGG   481 AAAACTTACC CTTAAATTTA TTTGCACTAC TGGAAAACTA CCTGTTCCGT GGCCAACACT   541 TGTCACTACT TTCTGTTATG GTGTTCAATG CTTTTCAAGA TACCCAGATC ACATGAAACA   601 GCATGACTTT TTCAAGAGTG CCATGCCCGA AGGCTATGTA CAGGAAAGAA CTATATTTTT   661 CAAAGATGAC GGGAACTACA AGACACGTGC TGAAGTCAAG TTTGAAGGTG ATACCCTTGT   721 TAATAGAATC GAGTTAAAAG GTATTGATTT TAAAGAAGAT GGAAACATTC TTGGACACAA   781 ATTGGAATAC AACTATAACT CACACAATGT ATACATCATG GCAGACAAAC AAAAGAATGG   841 AATCAAAGTT AACTTCAAAA TTAGACACAA CATTGAAGAT GGAAGCGTTC AACTAGCAGA   901 CCATTATCAA CAAAATACTC CAATTGGCGA TGGCCCTGTC CTTTTACCAG ACAACCATTA   961 CCTGTCCACA CAATCTGCCC TTTCGAAAGA TCCCAACGAA AAGAGAGACC ACATGGTCCT  1021 TTCTTGAGTT TGGTAACAGC TGCTGGGGAT TACACATGGG CATGGGATGA ACTTATACAA  1081 AGGATATCAC AAGTTGGTAA CAAAAAAAGC CTGAACGAGA AAACGTAAAA ATGAATATAA  1141 AAATATCACT ATTATAAGTA GAAATTTTTG CAATAAAAAA CACAGGAACC TTCACCGATA 1201 TAACTAACCT GGTGTATA //
Primer: nmt1Fw Sequence file: RDB12791_A4Eqb.seq
>06157_12791_A4Eq_ADHrev_A10_01.ab1     1 TGGAGGGGTA CTCAAGAATA AGAATTTTCG TTTTAAAACC TAAGGAGTCA CTTTAAAATT    61 TGTATACACT TATTTTTTTT ATAACTTATT TAATAATAAA AATCATAAAT CATAAGAAAT   121 TCGCTTATTT AGAAGTGGCG CGCCGGATCC TCTAGAGTCG AGATCTCATG ATATCACCAC   181 TTTGTACAAG AAAGCTGAAC GAGAAACGTA AAATGATATA AATATCAATA TATTAAATTA   241 GATTTTGCAT AAAAAACAGA CTACATAATA CTGTAAAACA CAACATATCC AGTCACTATG   301 GTCGACCTGC AGACTGGCTG TGTATAAGGG AGCCTGACAT TTATATTCCC CAGAACATCA   361 GGTTAATGGC GTTTTTGATG TCATTTTCGC GGTGGCTGAG ATCAGCCACT TCTTCCCCGA   421 TAACGGAGAC CGGCACACTG GCCATATCGG TGGTCATCAT GCGCCAGCTT TCATCCCCGA   481 TATGCACCAC CGGGTAAAGT TCACGGGAGA CTTTATCTGA CAGCAGACGT GCACTGGCCA   541 GGGGGATCAC CATCCGTCGC CCGGGCGTGT CAATAATATC ACTCTGTACA TCCACAAACA   601 GACGATAACG GCTCTCTCTT TTATAGGTGT AAACCTTAAA CTGCATTTCA CCAGCCCCTG   661 TTCTCGTCAG CAAAAGAGCC GTTCATTTCA ATAAACCGGG CGACCTCAGC CATCCCTTCC   721 TGATTTTCCG CTTTCCAGCG TTCGGCACGC AGACGACGGG CTTCATTCTG CATGGTTGTG   781 CTTACCAGAC CGGAGATATT GACATCATAT ATGCCTTGAG CAACTGATAG CTGTCGCTGT   841 CAACTGTCAC TGTAATACGC TGCTTCATAG CATACCTCTT TTTGACATAC TTCGGGTATA   901 CATATCAGTA TATATTCTTA TACCGCAAAA ATCAGCGCGC AAATACGCAT ACTGTTATCT   961 GGCTTTTAGT AAGCCGGGAT CCACGCGTTT ACGCCCCGCC CCTGCCACTC ATCGCAGTAC  1021 TGTTTGTAAT TTCATTAAGC ATTCTGCCGA CATGGAAGCC ATCACAAAAC GGGCATGATG  1081 GACTGATCGG CAGCGGCATC AGCACTGTCG CTGCGTATAA TATTTGGCCA TGGGTGGATA 1141 CGGGGGGGCG CTAG //
Primer: Reverse2 Sequence file: RDB12791_A4Eqc.seq
>06157_12791_A4Eq_Reverse2_C10_07.ab1     1 AATACATGAT TACGCCAGCT TGTGATATTG ACGAAACTTT TTGACATCTA ATTTATTCTG    61 TTCCAACACC AATGTTTATA ACCAAGTTTT ATCTTGTTTG TCTACATGGT ATTTTACATT   121 CATCTACATA CATCTTTCAT TGGCTTTGTA CATAGTTATC ATTACAAGTC TAAAAAAATT   181 CACTCTTTTC TTATTCAATG TCAATCCAAG AGAAAAGATT GTGGTAATGT TGTAGGAGCA   241 TGTTTAATAA ATTACTATAG CAAATTACTT TTTATTCCCA AGGTGTTTAT CTATAATAGT   301 TAATATTTTA GTCGCTACAT AAAATTTTAC CAAAGAGTAC TTGTATACTA ATTCTAAATG   361 CCTTCTGACA TAAAACGCCT AGGAAAACAA ACGCAAACAA GGCATCGACT TTTTCAATAA   421 CCAACCAAAA AAATTTTACA TTAGTCTTTT TTTAATGCTG AGAAAGTCTT TGCTGATATG   481 CCTTCCAACC AGCTTCTCTA TATCTCTTGG CTTCGACAAC AGGATTACGA CCAGCTCCAT   541 AGACTCCACG ACCAACAATG ATGATGTCGC TACCGCAGTT TACAATCACT TCTTCAGGAG   601 TACGATATTG CTGTCCCAGC CCGTCTCCTT TAACATCCAA GCCGATACCA GGGGACATAG   661 TTATGTAGTC GCTTTGAAGG TTAGGAAATC GACGACCAGC TATAAAGCCA AAGCAAAAAT   721 CGGTATGCTT CTCAAACCAT TCTAAGGTTT TCTCTGTGTA GGAACCAGTA GCCAAAGAGC   781 CTTTGGAAGA CATTTCAGCC AAAAGCAAGA GACCACGTCC CAAAGGTAAA CCAACTTCTT   841 TGAGGCCTTG TATAATACCC TCGCCTGGCA CTGTATGGCA ATTTGTGATA TGAGCCCAAG   901 AAGCAATTTT GTACACACCA GATGCATATT GTAGCTTGAC GGTATTTCCA ATGTCTGCGA   961 ATTTGCGATC CTCAAGATAA GAAAACGATG CTTTTTACCT AAGGCCACCA GTTTTTTCTA  1021 CCATATCCTG ATCGAAATCC TCGACAACGT CAATATGTGT CTGATAACAC AGACATAGGT  1081 CCAATTTTAT CTACATTTCT AGATTTCGGA TTCTCGTCGA TTCGACGCGA ACTGACAGCT 1141 GGCCTTGCTT TTCTTCCATC AAGGCCGAA //
Primer: Cm_M_R Sequence file: RDB12791_A4Eqd.seq
>06157_12791_A4Eq_Cm_M_R_D10_10.ab1     1 GGCGGGGGGT GAAACTATCC CATATCACCA GCTCACCGTC TTTCATTGCC ATACGGAATT    61 CCGGATGAGC ATTCATCAGG CGGGCAAGAA TGTGAATAAA GGCCGGATAA AACTTGTGCT   121 TATTTTTCTT TACGGTCTTT AAAAAGGCCG TAATATCCAG CTGAACGGTC TGGTTATAGG   181 TACATTGAGC AACTGACTGA AATGCCTCAA AATGTTCTTT ACGATGCCAT TGGGATATAT   241 CAACGGTGGT ATATCCAGTG ATTTTTTTCT CCATTTTAGC TTCCTTAGCT CCTGAAAATC   301 TCGACGGATC CTAACTCAAA ATCCACACAT TATACGAGCC GGAAGCATAA AGTGTAAAGC   361 CTGGGGTGCC TAATGCGGCC GCCATAGTGA CTGGATATGT TGTGTTTTAC AGTATTATGT   421 AGTCTGTTTT TTATGCAAAA TCTAATTTAA TATATTGATA TTTATATCAT TTTACGTTTC   481 TCGTTCAGCT TTTTTGTACA AACTTGTGAT ATCTTTGTAT AGTTCATCCA TGCCATGTGT   541 AATCCCAGCA GCTGTTACAA ACTCAAGAAG GACCATGTGG TCTCTCTTTT CGTTGGGATC   601 TTTCGAAAGG GCAGATTGTG TGGACAGGTA ATGGTTGTCT GGTAAAAGGA CAGGGCCATC   661 GCCAATTGGA GTATTTTGTT GATAATGGTC TGCTAGTTGA ACGCTTCCAT CTTCAATGTT   721 GTGTCTAATT TTGAAGTTAA CTTTGATTCC ATTCTTTTGT TTGTCTGCCA TGATGTATAC   781 ATTGTGTGAG TTATAGTTGT ATTCCAATTT GTGTCCAAGA ATGTTTCCAT CTTCTTTAAA   841 ATCAATACCT TTTAACTCGA TTCTATTAAC AAGGGTATCA CCTTCAAACT TTAACTTCAG   901 CACGTGTCTT GTAGTTCCCG TCATCTTTGA AAAATATAGT TCTTTCCTGT ACATAGCCTT   961 CGGGCATGGC ACTCTTGAAA AAGTCATGCT GTTTCATGTG ATCTGGGTAT CTTGAAAAGC  1021 ATTGAACACC ATAACAGAAA GTAGTGACAA GTGTTTGGCC ACGACAGTAG TTTTCCAGTA  1081 GTGCAATAAT TTAAGTAGTT TTCCCGTATG TGCATCAACT CAACCCTCTC CACTGACGGA 1141 AAATTGTGCC CAATTAACCA TCACCCATCC CA //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.07.13

GNP_filter3_RDBDEP_html_220713.pl