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pDUAL2-HFG1c

S. pombe 5 ter end His6-FLAG-GFP(S65C)-tagged vector with nmt1 promoter

Catalog number RDB06157
Resource name pDUAL2-HFG1c
Clone info. S. pombe 5 ter end His6-FLAG-GFP(S65C)-tagged vector with nmt1 promoter. Multicopy marker, ura4+; Integration marker, ura4+, leu1-.
Gateway compatible.
Vector backbone pDUAL2 (Plasmid)
Selectable markers E. coli: Amp^r, Cm^r, ccdB.
Growth remarks Use DB3.1, ccdB Survival or equivalent to amplify this clone. 30 degC is fine.
Reference of nucleotide sequence AB364086.1 (DDBJ accession number)
Depositor Yoshida, Minoru | Matsuyama, Akihisa |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
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Material Transfer Agreement (MTA) [Word]
Schedule A (GFP-A) [Link]
Remarks Use Survival2 (Invitrogen) or equivalent to amplify this clone.
Please use Schedule A (GFP-A) additionally.
Distribution information (domestic users) [open/close]


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RDB06157 pDUAL2-HFG1c DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB06157z.seq from Depositor

Original reference

original Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Expression Vector Backbone of Schizosaccharomyces pombe (English text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB12791_A4Eq.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: ADHrev | Sequence: RDB12791_A4Eqa.seq
>06157_12791_A4Eq_nmt1_Fw_B10_04.ab1
    1 ATGGATATTT TATTTAAATC AGGAAAAACG TAACTCTCGG CTACTGGATG GTTCAGTCAC
   61 CCAACGATTA CTGGGCGAGA GAAAACAGGG CAAAAGCAAA GCTTAAAGGA ATCCGATTGT
  121 CATTCGGCAA TGTGCAGCGA AACTAAAAAC CGGATAATGG ACCTGTTAAT CGAAACATTG
  181 AAGATATATA AAGGAAGAGG AATCCTGGCA TATCATCAAT TGAATAAGTT GAATTAATTA
  241 TTTCAATCTC ATTCTCACTT TCTGACTTAT AGTCGCTTTG TTAAAGCTAG CGTCGACAGG
  301 CCTGGATCCT ATGCATCACC ACCATCATCA TATGGATTAT AAGGATGATG ACGATAAACC
  361 CATGGGTAAA GGAGAAGAAC TTTTCACTGG AGTTGTCCCA ATTCTTGTTG AATTAGATGG
  421 TGATGTTAAT GGGCACAAAT TTTCTGTCAG TGGAGAGGGT GAAGGTGATG CAACATACGG
  481 AAAACTTACC CTTAAATTTA TTTGCACTAC TGGAAAACTA CCTGTTCCGT GGCCAACACT
  541 TGTCACTACT TTCTGTTATG GTGTTCAATG CTTTTCAAGA TACCCAGATC ACATGAAACA
  601 GCATGACTTT TTCAAGAGTG CCATGCCCGA AGGCTATGTA CAGGAAAGAA CTATATTTTT
  661 CAAAGATGAC GGGAACTACA AGACACGTGC TGAAGTCAAG TTTGAAGGTG ATACCCTTGT
  721 TAATAGAATC GAGTTAAAAG GTATTGATTT TAAAGAAGAT GGAAACATTC TTGGACACAA
  781 ATTGGAATAC AACTATAACT CACACAATGT ATACATCATG GCAGACAAAC AAAAGAATGG
  841 AATCAAAGTT AACTTCAAAA TTAGACACAA CATTGAAGAT GGAAGCGTTC AACTAGCAGA
  901 CCATTATCAA CAAAATACTC CAATTGGCGA TGGCCCTGTC CTTTTACCAG ACAACCATTA
  961 CCTGTCCACA CAATCTGCCC TTTCGAAAGA TCCCAACGAA AAGAGAGACC ACATGGTCCT
 1021 TTCTTGAGTT TGGTAACAGC TGCTGGGGAT TACACATGGG CATGGGATGA ACTTATACAA
 1081 AGGATATCAC AAGTTGGTAA CAAAAAAAGC CTGAACGAGA AAACGTAAAA ATGAATATAA
 1141 AAATATCACT ATTATAAGTA GAAATTTTTG CAATAAAAAA CACAGGAACC TTCACCGATA
1201 TAACTAACCT GGTGTATA
//
Primer: nmt1Fw | Sequence: RDB12791_A4Eqb.seq
>06157_12791_A4Eq_ADHrev_A10_01.ab1
    1 TGGAGGGGTA CTCAAGAATA AGAATTTTCG TTTTAAAACC TAAGGAGTCA CTTTAAAATT
   61 TGTATACACT TATTTTTTTT ATAACTTATT TAATAATAAA AATCATAAAT CATAAGAAAT
  121 TCGCTTATTT AGAAGTGGCG CGCCGGATCC TCTAGAGTCG AGATCTCATG ATATCACCAC
  181 TTTGTACAAG AAAGCTGAAC GAGAAACGTA AAATGATATA AATATCAATA TATTAAATTA
  241 GATTTTGCAT AAAAAACAGA CTACATAATA CTGTAAAACA CAACATATCC AGTCACTATG
  301 GTCGACCTGC AGACTGGCTG TGTATAAGGG AGCCTGACAT TTATATTCCC CAGAACATCA
  361 GGTTAATGGC GTTTTTGATG TCATTTTCGC GGTGGCTGAG ATCAGCCACT TCTTCCCCGA
  421 TAACGGAGAC CGGCACACTG GCCATATCGG TGGTCATCAT GCGCCAGCTT TCATCCCCGA
  481 TATGCACCAC CGGGTAAAGT TCACGGGAGA CTTTATCTGA CAGCAGACGT GCACTGGCCA
  541 GGGGGATCAC CATCCGTCGC CCGGGCGTGT CAATAATATC ACTCTGTACA TCCACAAACA
  601 GACGATAACG GCTCTCTCTT TTATAGGTGT AAACCTTAAA CTGCATTTCA CCAGCCCCTG
  661 TTCTCGTCAG CAAAAGAGCC GTTCATTTCA ATAAACCGGG CGACCTCAGC CATCCCTTCC
  721 TGATTTTCCG CTTTCCAGCG TTCGGCACGC AGACGACGGG CTTCATTCTG CATGGTTGTG
  781 CTTACCAGAC CGGAGATATT GACATCATAT ATGCCTTGAG CAACTGATAG CTGTCGCTGT
  841 CAACTGTCAC TGTAATACGC TGCTTCATAG CATACCTCTT TTTGACATAC TTCGGGTATA
  901 CATATCAGTA TATATTCTTA TACCGCAAAA ATCAGCGCGC AAATACGCAT ACTGTTATCT
  961 GGCTTTTAGT AAGCCGGGAT CCACGCGTTT ACGCCCCGCC CCTGCCACTC ATCGCAGTAC
 1021 TGTTTGTAAT TTCATTAAGC ATTCTGCCGA CATGGAAGCC ATCACAAAAC GGGCATGATG
 1081 GACTGATCGG CAGCGGCATC AGCACTGTCG CTGCGTATAA TATTTGGCCA TGGGTGGATA
1141 CGGGGGGGCG CTAG
//
Primer: Reverse2 | Sequence: RDB12791_A4Eqc.seq
>06157_12791_A4Eq_Reverse2_C10_07.ab1
    1 AATACATGAT TACGCCAGCT TGTGATATTG ACGAAACTTT TTGACATCTA ATTTATTCTG
   61 TTCCAACACC AATGTTTATA ACCAAGTTTT ATCTTGTTTG TCTACATGGT ATTTTACATT
  121 CATCTACATA CATCTTTCAT TGGCTTTGTA CATAGTTATC ATTACAAGTC TAAAAAAATT
  181 CACTCTTTTC TTATTCAATG TCAATCCAAG AGAAAAGATT GTGGTAATGT TGTAGGAGCA
  241 TGTTTAATAA ATTACTATAG CAAATTACTT TTTATTCCCA AGGTGTTTAT CTATAATAGT
  301 TAATATTTTA GTCGCTACAT AAAATTTTAC CAAAGAGTAC TTGTATACTA ATTCTAAATG
  361 CCTTCTGACA TAAAACGCCT AGGAAAACAA ACGCAAACAA GGCATCGACT TTTTCAATAA
  421 CCAACCAAAA AAATTTTACA TTAGTCTTTT TTTAATGCTG AGAAAGTCTT TGCTGATATG
  481 CCTTCCAACC AGCTTCTCTA TATCTCTTGG CTTCGACAAC AGGATTACGA CCAGCTCCAT
  541 AGACTCCACG ACCAACAATG ATGATGTCGC TACCGCAGTT TACAATCACT TCTTCAGGAG
  601 TACGATATTG CTGTCCCAGC CCGTCTCCTT TAACATCCAA GCCGATACCA GGGGACATAG
  661 TTATGTAGTC GCTTTGAAGG TTAGGAAATC GACGACCAGC TATAAAGCCA AAGCAAAAAT
  721 CGGTATGCTT CTCAAACCAT TCTAAGGTTT TCTCTGTGTA GGAACCAGTA GCCAAAGAGC
  781 CTTTGGAAGA CATTTCAGCC AAAAGCAAGA GACCACGTCC CAAAGGTAAA CCAACTTCTT
  841 TGAGGCCTTG TATAATACCC TCGCCTGGCA CTGTATGGCA ATTTGTGATA TGAGCCCAAG
  901 AAGCAATTTT GTACACACCA GATGCATATT GTAGCTTGAC GGTATTTCCA ATGTCTGCGA
  961 ATTTGCGATC CTCAAGATAA GAAAACGATG CTTTTTACCT AAGGCCACCA GTTTTTTCTA
 1021 CCATATCCTG ATCGAAATCC TCGACAACGT CAATATGTGT CTGATAACAC AGACATAGGT
 1081 CCAATTTTAT CTACATTTCT AGATTTCGGA TTCTCGTCGA TTCGACGCGA ACTGACAGCT
1141 GGCCTTGCTT TTCTTCCATC AAGGCCGAA
//
Primer: Cm_M_R | Sequence: RDB12791_A4Eqd.seq
>06157_12791_A4Eq_Cm_M_R_D10_10.ab1
    1 GGCGGGGGGT GAAACTATCC CATATCACCA GCTCACCGTC TTTCATTGCC ATACGGAATT
   61 CCGGATGAGC ATTCATCAGG CGGGCAAGAA TGTGAATAAA GGCCGGATAA AACTTGTGCT
  121 TATTTTTCTT TACGGTCTTT AAAAAGGCCG TAATATCCAG CTGAACGGTC TGGTTATAGG
  181 TACATTGAGC AACTGACTGA AATGCCTCAA AATGTTCTTT ACGATGCCAT TGGGATATAT
  241 CAACGGTGGT ATATCCAGTG ATTTTTTTCT CCATTTTAGC TTCCTTAGCT CCTGAAAATC
  301 TCGACGGATC CTAACTCAAA ATCCACACAT TATACGAGCC GGAAGCATAA AGTGTAAAGC
  361 CTGGGGTGCC TAATGCGGCC GCCATAGTGA CTGGATATGT TGTGTTTTAC AGTATTATGT
  421 AGTCTGTTTT TTATGCAAAA TCTAATTTAA TATATTGATA TTTATATCAT TTTACGTTTC
  481 TCGTTCAGCT TTTTTGTACA AACTTGTGAT ATCTTTGTAT AGTTCATCCA TGCCATGTGT
  541 AATCCCAGCA GCTGTTACAA ACTCAAGAAG GACCATGTGG TCTCTCTTTT CGTTGGGATC
  601 TTTCGAAAGG GCAGATTGTG TGGACAGGTA ATGGTTGTCT GGTAAAAGGA CAGGGCCATC
  661 GCCAATTGGA GTATTTTGTT GATAATGGTC TGCTAGTTGA ACGCTTCCAT CTTCAATGTT
  721 GTGTCTAATT TTGAAGTTAA CTTTGATTCC ATTCTTTTGT TTGTCTGCCA TGATGTATAC
  781 ATTGTGTGAG TTATAGTTGT ATTCCAATTT GTGTCCAAGA ATGTTTCCAT CTTCTTTAAA
  841 ATCAATACCT TTTAACTCGA TTCTATTAAC AAGGGTATCA CCTTCAAACT TTAACTTCAG
  901 CACGTGTCTT GTAGTTCCCG TCATCTTTGA AAAATATAGT TCTTTCCTGT ACATAGCCTT
  961 CGGGCATGGC ACTCTTGAAA AAGTCATGCT GTTTCATGTG ATCTGGGTAT CTTGAAAAGC
 1021 ATTGAACACC ATAACAGAAA GTAGTGACAA GTGTTTGGCC ACGACAGTAG TTTTCCAGTA
 1081 GTGCAATAAT TTAAGTAGTT TTCCCGTATG TGCATCAACT CAACCCTCTC CACTGACGGA
1141 AAATTGTGCC CAATTAACCA TCACCCATCC CA
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162.
user report Ito, D., Pericentrin-mediated SAS-6 recruitment promotes centriole assembly. Elife 8. pii: e41418 (2019). PMID 31182187.

2019.10.05

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