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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pDUAL2-HFG1c

S. pombe 5 ter end His6-FLAG-GFP(S65C)-tagged vector with nmt1 promoter

Catalog number RDB06157
Resource name pDUAL2-HFG1c
Clone info. S. pombe 5 ter end His6-FLAG-GFP(S65C)-tagged vector with nmt1 promoter. Multicopy marker, ura4+; Integration marker, ura4+, leu1-.
Comment Gateway compatible.
Vector backbone pDUAL2 (Plasmid)
Selectable markers E. coli: Amp^r, Cm^r, ccdB.
Growth remarks Use DB3.1, ccdB Survival or equivalent to amplify this clone. 30 degC is fine.
Reference of insert sequence AB364086.1 (DDBJ accession number)
Depositor|Developer Yoshida, Minoru | Matsuyama, Akihisa |
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External Database

            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
            Ordering Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
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            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
            Remarks Use Survival2 (Invitrogen) or equivalent to amplify this clone.
            提供案内 (日本国内) [open/close]

            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) の引用と、 寄託者への謝辞の表明を必要とする。 利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。 寄託者から要請がある場合、理研BRCは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
            GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします (Schedule AとSchedule Bを入手[link])。
            備考 このクローンの増殖にはSurvival2 (Invitrogen) あるいは同等の宿主を使用してください。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB06157 pDUAL2-HFG1c DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Sequence RDB06157z.seq

            Original reference

            original Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Expression Vector Backbone of Schizosaccharomyces pombe (English text)

            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB12791_A4Eq.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: ADHrev
            Sequence file: RDB12791_A4Eqa.seq check
            >06157_12791_A4Eq_nmt1_Fw_B10_04.ab1
                1 ATGGATATTT TATTTAAATC AGGAAAAACG TAACTCTCGG CTACTGGATG GTTCAGTCAC
               61 CCAACGATTA CTGGGCGAGA GAAAACAGGG CAAAAGCAAA GCTTAAAGGA ATCCGATTGT
              121 CATTCGGCAA TGTGCAGCGA AACTAAAAAC CGGATAATGG ACCTGTTAAT CGAAACATTG
              181 AAGATATATA AAGGAAGAGG AATCCTGGCA TATCATCAAT TGAATAAGTT GAATTAATTA
              241 TTTCAATCTC ATTCTCACTT TCTGACTTAT AGTCGCTTTG TTAAAGCTAG CGTCGACAGG
              301 CCTGGATCCT ATGCATCACC ACCATCATCA TATGGATTAT AAGGATGATG ACGATAAACC
              361 CATGGGTAAA GGAGAAGAAC TTTTCACTGG AGTTGTCCCA ATTCTTGTTG AATTAGATGG
              421 TGATGTTAAT GGGCACAAAT TTTCTGTCAG TGGAGAGGGT GAAGGTGATG CAACATACGG
              481 AAAACTTACC CTTAAATTTA TTTGCACTAC TGGAAAACTA CCTGTTCCGT GGCCAACACT
              541 TGTCACTACT TTCTGTTATG GTGTTCAATG CTTTTCAAGA TACCCAGATC ACATGAAACA
              601 GCATGACTTT TTCAAGAGTG CCATGCCCGA AGGCTATGTA CAGGAAAGAA CTATATTTTT
              661 CAAAGATGAC GGGAACTACA AGACACGTGC TGAAGTCAAG TTTGAAGGTG ATACCCTTGT
              721 TAATAGAATC GAGTTAAAAG GTATTGATTT TAAAGAAGAT GGAAACATTC TTGGACACAA
              781 ATTGGAATAC AACTATAACT CACACAATGT ATACATCATG GCAGACAAAC AAAAGAATGG
              841 AATCAAAGTT AACTTCAAAA TTAGACACAA CATTGAAGAT GGAAGCGTTC AACTAGCAGA
              901 CCATTATCAA CAAAATACTC CAATTGGCGA TGGCCCTGTC CTTTTACCAG ACAACCATTA
              961 CCTGTCCACA CAATCTGCCC TTTCGAAAGA TCCCAACGAA AAGAGAGACC ACATGGTCCT
             1021 TTCTTGAGTT TGGTAACAGC TGCTGGGGAT TACACATGGG CATGGGATGA ACTTATACAA
             1081 AGGATATCAC AAGTTGGTAA CAAAAAAAGC CTGAACGAGA AAACGTAAAA ATGAATATAA
             1141 AAATATCACT ATTATAAGTA GAAATTTTTG CAATAAAAAA CACAGGAACC TTCACCGATA
            1201 TAACTAACCT GGTGTATA
            //
            Primer: nmt1Fw
            Sequence file: RDB12791_A4Eqb.seq check
            >06157_12791_A4Eq_ADHrev_A10_01.ab1
                1 TGGAGGGGTA CTCAAGAATA AGAATTTTCG TTTTAAAACC TAAGGAGTCA CTTTAAAATT
               61 TGTATACACT TATTTTTTTT ATAACTTATT TAATAATAAA AATCATAAAT CATAAGAAAT
              121 TCGCTTATTT AGAAGTGGCG CGCCGGATCC TCTAGAGTCG AGATCTCATG ATATCACCAC
              181 TTTGTACAAG AAAGCTGAAC GAGAAACGTA AAATGATATA AATATCAATA TATTAAATTA
              241 GATTTTGCAT AAAAAACAGA CTACATAATA CTGTAAAACA CAACATATCC AGTCACTATG
              301 GTCGACCTGC AGACTGGCTG TGTATAAGGG AGCCTGACAT TTATATTCCC CAGAACATCA
              361 GGTTAATGGC GTTTTTGATG TCATTTTCGC GGTGGCTGAG ATCAGCCACT TCTTCCCCGA
              421 TAACGGAGAC CGGCACACTG GCCATATCGG TGGTCATCAT GCGCCAGCTT TCATCCCCGA
              481 TATGCACCAC CGGGTAAAGT TCACGGGAGA CTTTATCTGA CAGCAGACGT GCACTGGCCA
              541 GGGGGATCAC CATCCGTCGC CCGGGCGTGT CAATAATATC ACTCTGTACA TCCACAAACA
              601 GACGATAACG GCTCTCTCTT TTATAGGTGT AAACCTTAAA CTGCATTTCA CCAGCCCCTG
              661 TTCTCGTCAG CAAAAGAGCC GTTCATTTCA ATAAACCGGG CGACCTCAGC CATCCCTTCC
              721 TGATTTTCCG CTTTCCAGCG TTCGGCACGC AGACGACGGG CTTCATTCTG CATGGTTGTG
              781 CTTACCAGAC CGGAGATATT GACATCATAT ATGCCTTGAG CAACTGATAG CTGTCGCTGT
              841 CAACTGTCAC TGTAATACGC TGCTTCATAG CATACCTCTT TTTGACATAC TTCGGGTATA
              901 CATATCAGTA TATATTCTTA TACCGCAAAA ATCAGCGCGC AAATACGCAT ACTGTTATCT
              961 GGCTTTTAGT AAGCCGGGAT CCACGCGTTT ACGCCCCGCC CCTGCCACTC ATCGCAGTAC
             1021 TGTTTGTAAT TTCATTAAGC ATTCTGCCGA CATGGAAGCC ATCACAAAAC GGGCATGATG
             1081 GACTGATCGG CAGCGGCATC AGCACTGTCG CTGCGTATAA TATTTGGCCA TGGGTGGATA
            1141 CGGGGGGGCG CTAG
            //
            Primer: Reverse2
            Sequence file: RDB12791_A4Eqc.seq check
            >06157_12791_A4Eq_Reverse2_C10_07.ab1
                1 AATACATGAT TACGCCAGCT TGTGATATTG ACGAAACTTT TTGACATCTA ATTTATTCTG
               61 TTCCAACACC AATGTTTATA ACCAAGTTTT ATCTTGTTTG TCTACATGGT ATTTTACATT
              121 CATCTACATA CATCTTTCAT TGGCTTTGTA CATAGTTATC ATTACAAGTC TAAAAAAATT
              181 CACTCTTTTC TTATTCAATG TCAATCCAAG AGAAAAGATT GTGGTAATGT TGTAGGAGCA
              241 TGTTTAATAA ATTACTATAG CAAATTACTT TTTATTCCCA AGGTGTTTAT CTATAATAGT
              301 TAATATTTTA GTCGCTACAT AAAATTTTAC CAAAGAGTAC TTGTATACTA ATTCTAAATG
              361 CCTTCTGACA TAAAACGCCT AGGAAAACAA ACGCAAACAA GGCATCGACT TTTTCAATAA
              421 CCAACCAAAA AAATTTTACA TTAGTCTTTT TTTAATGCTG AGAAAGTCTT TGCTGATATG
              481 CCTTCCAACC AGCTTCTCTA TATCTCTTGG CTTCGACAAC AGGATTACGA CCAGCTCCAT
              541 AGACTCCACG ACCAACAATG ATGATGTCGC TACCGCAGTT TACAATCACT TCTTCAGGAG
              601 TACGATATTG CTGTCCCAGC CCGTCTCCTT TAACATCCAA GCCGATACCA GGGGACATAG
              661 TTATGTAGTC GCTTTGAAGG TTAGGAAATC GACGACCAGC TATAAAGCCA AAGCAAAAAT
              721 CGGTATGCTT CTCAAACCAT TCTAAGGTTT TCTCTGTGTA GGAACCAGTA GCCAAAGAGC
              781 CTTTGGAAGA CATTTCAGCC AAAAGCAAGA GACCACGTCC CAAAGGTAAA CCAACTTCTT
              841 TGAGGCCTTG TATAATACCC TCGCCTGGCA CTGTATGGCA ATTTGTGATA TGAGCCCAAG
              901 AAGCAATTTT GTACACACCA GATGCATATT GTAGCTTGAC GGTATTTCCA ATGTCTGCGA
              961 ATTTGCGATC CTCAAGATAA GAAAACGATG CTTTTTACCT AAGGCCACCA GTTTTTTCTA
             1021 CCATATCCTG ATCGAAATCC TCGACAACGT CAATATGTGT CTGATAACAC AGACATAGGT
             1081 CCAATTTTAT CTACATTTCT AGATTTCGGA TTCTCGTCGA TTCGACGCGA ACTGACAGCT
            1141 GGCCTTGCTT TTCTTCCATC AAGGCCGAA
            //
            Primer: Cm_M_R
            Sequence file: RDB12791_A4Eqd.seq check
            >06157_12791_A4Eq_Cm_M_R_D10_10.ab1
                1 GGCGGGGGGT GAAACTATCC CATATCACCA GCTCACCGTC TTTCATTGCC ATACGGAATT
               61 CCGGATGAGC ATTCATCAGG CGGGCAAGAA TGTGAATAAA GGCCGGATAA AACTTGTGCT
              121 TATTTTTCTT TACGGTCTTT AAAAAGGCCG TAATATCCAG CTGAACGGTC TGGTTATAGG
              181 TACATTGAGC AACTGACTGA AATGCCTCAA AATGTTCTTT ACGATGCCAT TGGGATATAT
              241 CAACGGTGGT ATATCCAGTG ATTTTTTTCT CCATTTTAGC TTCCTTAGCT CCTGAAAATC
              301 TCGACGGATC CTAACTCAAA ATCCACACAT TATACGAGCC GGAAGCATAA AGTGTAAAGC
              361 CTGGGGTGCC TAATGCGGCC GCCATAGTGA CTGGATATGT TGTGTTTTAC AGTATTATGT
              421 AGTCTGTTTT TTATGCAAAA TCTAATTTAA TATATTGATA TTTATATCAT TTTACGTTTC
              481 TCGTTCAGCT TTTTTGTACA AACTTGTGAT ATCTTTGTAT AGTTCATCCA TGCCATGTGT
              541 AATCCCAGCA GCTGTTACAA ACTCAAGAAG GACCATGTGG TCTCTCTTTT CGTTGGGATC
              601 TTTCGAAAGG GCAGATTGTG TGGACAGGTA ATGGTTGTCT GGTAAAAGGA CAGGGCCATC
              661 GCCAATTGGA GTATTTTGTT GATAATGGTC TGCTAGTTGA ACGCTTCCAT CTTCAATGTT
              721 GTGTCTAATT TTGAAGTTAA CTTTGATTCC ATTCTTTTGT TTGTCTGCCA TGATGTATAC
              781 ATTGTGTGAG TTATAGTTGT ATTCCAATTT GTGTCCAAGA ATGTTTCCAT CTTCTTTAAA
              841 ATCAATACCT TTTAACTCGA TTCTATTAAC AAGGGTATCA CCTTCAAACT TTAACTTCAG
              901 CACGTGTCTT GTAGTTCCCG TCATCTTTGA AAAATATAGT TCTTTCCTGT ACATAGCCTT
              961 CGGGCATGGC ACTCTTGAAA AAGTCATGCT GTTTCATGTG ATCTGGGTAT CTTGAAAAGC
             1021 ATTGAACACC ATAACAGAAA GTAGTGACAA GTGTTTGGCC ACGACAGTAG TTTTCCAGTA
             1081 GTGCAATAAT TTAAGTAGTT TTCCCGTATG TGCATCAACT CAACCCTCTC CACTGACGGA
            1141 AAATTGTGCC CAATTAACCA TCACCCATCC CA
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162.
            user_report Ito, D., Pericentrin-mediated SAS-6 recruitment promotes centriole assembly. Elife 8. pii: e41418 (2019). PMID 31182187.

            2022.05.18

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