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pDUAL-YFH41c

S. pombe 3 ter end YFP-FLAG-His6-tagged vector with nmt1 (medium strength) promoter

Catalog number RDB06152
Resource name pDUAL-YFH41c
Clone info. S. pombe 3 ter end YFP-FLAG-His6-tagged vector with nmt1 (medium strength) promoter. Multicopy marker, ura4+; Integration marker, leu1+.
Comment Gateway compatible.
Vector backbone pDUAL (Plasmid)
Selectable markers E. coli: Amp^r, Cm^r, ccdB.
Growth remarks Use DB3.1, ccdB Survival or equivalent to amplify this clone. 30 degC is fine.
Reference of insert sequence AB364105.1 (DDBJ accession number)
Depositor|Developer Yoshida, Minoru | Matsuyama, Akihisa |
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External Database

            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature(s). In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must state an acknowledgment to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR . When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
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            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
            Remarks Use Survival2 (Invitrogen) or equivalent to amplify this clone.
            提供案内 (日本国内) [open/close]

            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献の引用と、寄託者への謝辞の表明を必要とする。 利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。 寄託者から要請がある場合、理研BRCは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
            GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします (Schedule AとSchedule Bを入手[link])。
            備考 このクローンの増殖にはSurvival2 (Invitrogen) あるいは同等の宿主を使用してください。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB06152 pDUAL-YFH41c DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Sequence RDB06152z.seq

            Original reference

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Expression Vector Backbone of Schizosaccharomyces pombe (English text)

            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB13464_A5G7p1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: nmt1Fw
            Sequence file: RDB13464_A5G7a.seq check
            >06152_13464_A5G7_nmt1Fw_A10_01.ab1
                1 TGGAGTTATG GAATCAGGAA AACGTAACTC TCGGCTACTG GATGGTTCAG TCACCCAACG
               61 ATTACTGGGG AGAGAAAACA GGGCAAAAGC AAAGCTTAAA GGAATCCGAT TGTCATTCGG
              121 CAATGTGCAG CGAAACTAAA AACCGGATAA TGGACCTGTT AATCGAAACA TTGAAGATAA
              181 AGGAAGAGGA ATCCTGGCAT ATCATCAATT GAATAAGTTG AATTAATTAT TTCAATCTCA
              241 TTCTCACTTT CTGACTTATA GTCGCTTTGT TAAAGCTAGC GTCGACAGGC CTGGATCCGG
              301 ATATCAACAA GTTTGTACAA AAAAGCTGAA CGAGAAACGT AAAATGATAT AAATATCAAT
              361 ATATTAAATT AGATTTTGCA TAAAAAACAG ACTACATAAT ACTGTAAAAC ACAACATATC
              421 CAGTCACTAT GGCGGCCGCA TTAGGCACCC CAGGCTTTAC ACTTTATGCT TCCGGCTCGT
              481 ATAATGTGTG GATTTTGAGT TAGGATCCGT CGAGATTTTC AGGAGCTAAG GAAGCTAAAA
              541 TGGAGAAAAA AATCACTGGA TATACCACCG TTGATATATC CCAATGGCAT CGTAAAGAAC
              601 ATTTTGAGGC ATTTCAGTCA GTTGCTCAAT GTACCTATAA CCAGACCGTT CAGCTGGATA
              661 TTACGGCCTT TTTAAAGACC GTAAAGAAAA ATAAGCACAA GTTTTATCCG GCCTTTATTC
              721 ACATTCTTGC CCGCCTGATG AATGCTCATC CGGAATTCCG TATGGCAATG AAAGACGGTG
              781 AGCTGGTGAT ATGGGATAGT GTTCACCCTT GTTACACCGT TTTCCATGAG CAAACTGAAA
              841 CGTTTTCATC GCTCTGGAGT GAATACCACG ACGATATCCG GCAGTTTCTA CACATATATT
              901 CGCAAGATGT GGCGTGTTAC GGTGAAAACC TGGCCTATTT CCCTAAAGGG TTTATTGAGA
              961 ATATGTTTTT TCGTCTCAGC CCAATCCCTG GTGAGTTTCA CCAGTTTTGA TTTAAACGTG
             1021 GGCCAATATG GGACACTTCT TCGCCCCCGG TTTCACATGG GCAATATATA CGCAAGGCCG
            1081 ACAGTGCTGA TGGCCCGCTT GGGCGATTCC AGGCTTTCCA T
            //
            Primer: YFP_R
            Sequence file: RDB13464_A5G7b.seq check
            >06152_13464_A5G7_YFP_R1_B10_04.ab1
                1 GAGCTGCATG TCTCGCCGGA ACGCTGACTT GTGGCCGTTT ACGTCGCCGT CCAGCTCGAC
               61 CAGGATGGGC ACCACCCCGG TGAACAGCTC CTCGCCCTTG CTCACCATGA TATCAACCAC
              121 TTTGTACAAG AAAGCTGAAC GAGAAACGTA AAATGATATA AATATCAATA TATTAAATTA
              181 GATTTTGCAT AAAAAACAGA CTACATAATA CTGTAAAACA CAACATATCC AGTCACTATG
              241 GTCGACCTGC AGACTGGCTG TGTATAAGGG AGCCTGACAT TTATATTCCC CAGAACATCA
              301 GGTTAATGGC GTTTTTGATG TCATTTTCGC GGTGGCTGAG ATCAGCCACT TCTTCCCCGA
              361 TAACGGAGAC CGGCACACTG GCCATATCGG TGGTCATCAT GCGCCAGCTT TCATCCCCGA
              421 TATGCACCAC CGGGTAAAGT TCACGGGAGA CTTTATCTGA CAGCAGACGT GCACTGGCCA
              481 GGGGGATCAC CATCCGTCGC CCGGGCGTGT CAATAATATC ACTCTGTACA TCCACAAACA
              541 GACGATAACG GCTCTCTCTT TTATAGGTGT AAACCTTAAA CTGCATTTCA CCAGCCCCTG
              601 TTCTCGTCAG CAAAAGAGCC GTTCATTTCA ATAAACCGGG CGACCTCAGC CATCCCTTCC
              661 TGATTTTCCG CTTTCCAGCG TTCGGCACGC AGACGACGGG CTTCATTCTG CATGGTTGTG
              721 CTTACCAGAC CGGAGATATT GACATCATAT ATGCCTTGAG CAACTGATAG CTGTCGCTGT
              781 CAACTGTCAC TGTAATACGC TGCTTCATAG CATACCTCTT TTTGACATAC TTCGGGTATA
              841 CATATCAGTA TATATTCTTA TACCGCAAAA ATCAGCGCGC AAATACGCAT ACTGTTATCT
              901 GGCTTTTAGT AAGCCGGATC CAGATCTTTA CGCCCCGCCC TGCCACTCAT CGCAGTACTG
              961 TTGTAATTCA TTAAGCATTC TGCCGACATG GAAAGCCATC ACAAACGGCA TGATGAAACC
             1021 TGAATCGCCC AGCGGGCATC AGCACCCTTG TCGCCTTTGC GTATATATTT TGCCCAATGA
            1081 TGAAACCGGG GGCCGCAGAG TTGATCCATT ATTTGACCCA CCGGATTTA
            //
            Primer: ADH_rev
            Sequence file: RDB13464_A5G7c.seq check
            >06152_13464_A5G7_ADHrev_C10_07.ab1
                1 TGAGAATACT CAAGATAAGA TTTTCGTTTT AAAACCTAAG AGTCACTTTA AAATTTGTAT
               61 ACACTTATTT TTTTTATAAC TTATTTAATA ATAAAAATCA TAAATCATAA GAAATTCGCT
              121 TATTTAGAAG TGGCGCGCCG GATCCTCTAG AGTCGAGATC TCAGTGGTGA TGGTGGTGAT
              181 GTTTGTCATC GTCGTCCTTG TAGTCCATGG GCTTGTACAG CTCGTCCATG CCGAGAGTGA
              241 TCCCGGCGGC GGTCACGAAC TCCAGCAGGA CCATGTGATC GCGCTTCTCG TTGGGGTCTT
              301 TGCTCAGGGC GGACTGGTAG CTCAGGTAGT GGTTGTCGGG CAGCAGCACG GGGCCGTCGC
              361 CGATGGGGGT GTTCTGCTGG TAGTGGTCGG CGAGCTGCAC GCTGCCGTCC TCGATGTTGT
              421 GGCGGATCTT GAAGTTCACC TTGATGCCGT TCTTCTGCTT GTCGGCCATG ATATAGACGT
              481 TGTGGCTGTT GTAGTTGTAC TCCAGCTTGT GCCCCAGGAT GTTGCCGTCC TCCTTGAAGT
              541 CGATGCCCTT CAGCTCGATG CGGTTCACCA GGGTGTCGCC CTCGAACTTC ACCTCGGCGC
              601 GGGTCTTGTA GTTGCCGTCG TCCTTGAAGA AGATGGTGCG CTCCTGGACG TAGCCTTCGG
              661 GCATGGCGGA CTTGAAGAAG TCGTGCTGCT TCATGTGGTC GGGGTAGCGG GCGAAGCACT
              721 GCAGGCCGTA GCCGAAGGTG GTCACGAGGG TGGGCCAGGG CACGGGCAGC TTGCCGGTGG
              781 TGCAGATGAA CTTCAGGGTC AGCTTGCCGT AGGTGGCATC GCCCTCGCCC TCGCCGGACA
              841 CGCTGAACTT GTGGCCGTTT ACGTCGCCGT CCAGCTCGAC CAGGATGGGC ACCACCCCCG
              901 GTGAACAGCT CCTCGCCCTT GCTCACCATG ATATCAACCA CTTTTGTACA AGAAAAGCTG
              961 AAACGAGAAA CGTAAAAATG AATAATAAAT ATCAATATAT TAAAATTTAG AATTTTTGCA
             1021 TAAAAAAAAC CAGACTACCA TAATAACTGT TAAAAACACA AACAATATCC AGTTCACTAT
            1081 GATCGGACCC TTGCCAGAAC TGGCTTGTGT CTTATAATTA ACG
            //
            Primer: ADH-Forward
            Sequence file: RDB13464_A5G7d.seq check
            >06152_13464_A5G7_ADH_Forward_D10_10.ab1
                1 AGCTCCGTAT TGACACACCT CTACCGGCAG ATCCGCTAGG GATAACAGGG TAATATACTA
               61 GTAAGATGAT TCTTTTATCG TCGATAACGA TGAGGATGTA GACGATTATG ATGAGAGTGA
              121 TTAGCTTCTA AAATTTGGTG TTTTCAAACG CTATTTACAG TACAATCTGA AAAAATTGGA
              181 TGAACATTGT AAATGGTAGG ATACGAGTTC CAATTAATGT ATTTTTTAAG TATTACACTT
              241 AGCTTATTAA TTTTATATAT ACATTCTACC TCTGTGGGTT TAAAGACAAC TCGTACACAT
              301 ACTCAAATTA TTTATGAACA TTATAATGCA TATTCGTACT GTCTATTAAA CATTTTTTTG
              361 ACATTAAAAA TTTCGTTTAC TAACGTAGAA AGCGCTGATA ATTGAGGCTA CTATTTTAAT
              421 ATCTAGAAAC AAAGATTCAT TAATTAATTA AGTAAGCTCC AGGATACTTG TATATTTCGT
              481 TAAAAAAGCG CAATTTCAAC AATTCCTATG AACATCCATG ATTTCATCAC AAAATCAAAC
              541 ATATCAGGAT TTAACGTGAA TTATTTGCTT TCTTGTGATT TTCATCTGAT TTCTGGTCAT
              601 TTACGTTACT GTAAACAAGC TTTTTAAAAA AAGGATTTTT TACGCAATAT TATCGATATC
              661 CCAATCTGTA GTATAAAAAG TGCAACAGTG TTGAGTTTCC CGAAACCAGG CATTTTTGCG
              721 GTAGGGTCTG AAACTTTCAC GAAACCATTT CACAACAAAA CTTCTTCAAC AACATGTGTG
              781 CAAAGAAAAT CGTCGTCTTA CCAGGAGACC ATATTGGCCC TGAAATTGTT GCTTCTGCCT
              841 TGGAGGTTTT GAAAGTCGTT GAGAAGAAGC GACCTGAGTT AAAACTCGAG TTTGAAGAAC
              901 ACAAGATTGG AGGTGCCTCT ATTGATGCCT ATGGAACCCC TTTGACTGAC GAGACTGTGA
              961 AAGGCTTGTT TGGAAGCTGA CGGTGTTCTT TTGGGTGCCG TTGGTGGTCC TGAATGGACC
             1021 CAACCCCATT GTTCGTCTGA AGCACGTTTT AATGGAGGCT CCGTTAGAAG TATGGGTGTT
            1081 CGGCCAACCA TCGACCATGC CACCTTTGCC AGCCAAGTTC GATTTAG
            //
            Primer: ars_R1
            Sequence file: RDB13464_A5G7e.seq check
            >06152_13464_A5G7_ars_R1_E10_13.ab1
                1 TGGTTCGGTT TATACTATTA CTTAAGTACC TTTAACTAAA CAAAATGCCT ATATATATAT
               61 TAATTTACAA TGAGTGTCAG ATAAGTCACT ATGTCCGAGT GGTTAAGGAG TTAGACTCGA
              121 ATTCGCAAGC TTAGCTACAA ATCCCACTGG CTATATGTAT GCATTTGTGT TAAAAAAGTT
              181 TGTATAGATT ATTTAATCTA CTCAGCATTC TTTCTCTAAA TAGGAATTTG TTACTTAATG
              241 GAGAAAAAAA TGTTTCGATT TACCTAGTGT ATTTGTTTGT ATACTCACGT TTAATTTCAA
              301 ACATCCATTC TATCTTGTGT AATTTTTGGC ATGGTGAAAA AGATAATCAG CCTTATAATC
              361 TTTACAAAAG TAAGAAATTC TGTAAATAAG CCTTAATGCC CTTGCTTTAA ATTAAAATGG
              421 TTCTTTTTCA TGATAATGTT TGCACTTTGT GAATATATTT TAGATAGTTC TGTGAGGTAT
              481 AATTAAGATG TTTTAGAGAC TTATACAATT TTGTCTTTAT AAATTCTTAA TTGATTTTAC
              541 CATCCCAGTT TAACTATGCT TCGTCGGCAT CTCTGCACAT GTCGTGTTTT CTTACCGTAT
              601 TGTCCTACCA AGAACCTCTT TTTTGCTTGG ATCGAAATTA AAGGTTTAAA AGCAAAGTTA
              661 TGGATGCTAG AGTATTTCAA AGCTATTCAG CTAGAGCTGA GGGGATGAAA AATCCCATTG
              721 CCAAGGAATT GTTGGCTTTG ATGGAAGAAA AGCAAAGCAA CTTGTCAGTC GCGGTCGATT
              781 TGACGAAGAA ATCCGAAATC TTAGAATTGG TAGATAAAAT TGGACCCTAT GTCTGTGTTA
              841 TCAAGACACA TATTGACGTT GTCGAGGATT TCGACCAGGA TATGGTAGAA AAACTGGTGG
              901 CCTTAGTAAA AAGCATCGTT TTCTTATCTT TGAGGATCGC AAATTCGCAG ACATTGGAAA
              961 TACCGTCAAG CTACAATATG CATCTGGGTG TGTACAAAAT TTGCTTCTTG GGCTCATATC
             1021 ACAATTTGCC ATACAGTGCC CAGCGAGGGG TATTATTACC AAGTCCTCAA AAGAAGGTTG
            1081 ATTAACCGTT GGACGTTGAT CTCTTGGCTT TTTGGCTGGA AATGTTCCTT TCCAAG
            //
            Primer: f1ori_R3
            Sequence file: RDB13464_A5G7f.seq check
            >06152_13464_A5G7_f1ori_R3_F10_16.ab1
                1 GAATGGGTCT TGCGTATGCG GTGTGAATAC CGCACAGATG CGTAAGGAGA AAATACCGCA
               61 TCAGGCGCCA TTCGCCATTC AGGCTGCGCA ACTGTTGGGA AGGGCGATCG GTGCGGGCCT
              121 CTTCGCTATT ACGCCAGCTG GCGAAAGGGG GATGTGCTGC AAGGCGATTA AGTTGGGTAA
              181 CGCCAGGGTT TTCCCAGTCA CGACGTTGTA AAACGACGGC CAGTGAATTC GAGCTCGGGA
              241 GCGCTACCGT GAATGGGCTC AACCAAACAA TGAACCTTTT CTTCTGATTT TCCTACCACA
              301 CCGGAAAGGG AGGCAGAAGG CAAAAGGCCC AAGCTACCAG GAATGACAGA AGCCTCATCT
              361 GAAATAATGT CACCAAACAA GTTGTCAGTC AAAACAACAC CGTTAAGTGT ACGAGGGCTC
              421 TTGACCAAAA GCATGGCTGC GGAGTCAATG AGCTGGTTTT TTAAGGTAAG GTGAGGATAT
              481 TCCTCCTTAA AAATCTTAGC TACAGTCTTG CGCCAAAGAC GAGAAGTTGC CAAAACATTA
              541 GCTTTGTCGA GTAATGTGAC GGGAGCAGGA GGGTTGGAAG TTTCAGCTAA GCGGCCGCGG
              601 GCCCAACCTT CCAATTCATT AAATCGAGTC CTCCATCAGA TGATTTACAA GGTTTTGCAT
              661 AGAATCCCTA AGTAAGAGAA GTTGTTAGTG ATTTCTTCAA GTTTACTAAA ATTTACATAC
              721 AAATGGATGA TCTCTTCTCC ATTGTTTCCT TTCTTCTTGC AAACGCGTTT TACAAAGAGA
              781 TGACATGCTA TTTTCTGATT ATTTTTTTCT ATAGTTTTCT ATTTTGTACG TCCTTGGGAG
              841 TCCGAAATGT AAAATCGGGG GTATGGTTAG TAGCGGCAGC GTTCATTATG GTATATAAAA
              901 GATAAAAAGT AGGGGGCAAG CGAGACAGAA TGGGATACAA GGGCATCGTC TATAATTATA
              961 GCTAAAAATT GTATTTTAAT TTGTATTTTT TTTGTAATTT TATTTCTCAT TTGTTTTACT
             1021 TTAAAATGAT GCGAAATTTA GAGAAAACTT CACCGAAATG TCCAAAATAA GCCTCAGCAC
            1081 ATATACAAGT TTTAGGAAAG CGATCAGCAA CCTTTAA
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            user_report Zhang, M.M., Quantitative control of protein S-palmitoylation regulates meiotic entry in fission yeast. PLoS Biol., 11 (7): e1001597 (2013). PMID 23843742.

            2022.07.13

            GNP_filter3_RDBDEP_html_220713.pl