RDB06151 - pDUAL-YFH1c

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pDUAL-YFH1c (#RDB06151)

S. pombe 3 ter end YFP-FLAG-His6-tagged vector with nmt1 promoter

Clone info.	S. pombe 3 ter end YFP-FLAG-His6-tagged vector with nmt1 promoter. Multicopy marker, ura4+; Integration marker, leu1+.
Comment	Gateway compatible.
Vector backbone	pDUAL (RDB06178) (Plasmid)
Selectable markers	E. coli: Amp^r, Cm^r, ccdB.
Growth remarks	Use DB3.1, ccdB Survival or equivalent to amplify this clone. 30 degC is fine.
Reference of insert sequence	AB364104.1 (DDBJ accession number)
Depositor\|Developer	Yoshida, Minoru \| Matsuyama, Akihisa \|

Sequence	RDB06151z.seq

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature(s). In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must state an acknowledgment to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR . When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.

Remarks Use Survival2 (Invitrogen) or equivalent to amplify this clone.

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Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.
Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature(s). In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must state an acknowledgment to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR . When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
Remarks	Use Survival2 (Invitrogen) or equivalent to amplify this clone.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件	利用者は、研究成果の公表にあたって寄託者の指定する文献の引用と、寄託者への謝辞の表明を必要とする。　利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。　寄託者から要請がある場合、理研ＢＲＣは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。
備考	このクローンの増殖にはSurvival2 (Invitrogen) あるいは同等の宿主を使用してください。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB06151	pDUAL-YFH1c	DNA solution

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pDUAL-YFH1c was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB06151).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB08940_A5Fgp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: nmt1Fw Sequence file: RDB08940_A5Fga.seq
>06151_08940_A5Fg_nmt1Fw_D01_10.ab1 1 GGAATTTTAT TAATCAGGGA AAACGTAACT CTCGGCTACT GGATGGTTCA GTCACCCAAC 61 GATTACTGGG GAGAGAAAAC AGGGCAAAAG CAAAGCTTAA AGGAATCCGA TTGTCATTCG 121 GCAATGTGCA GCGAAACTAA AAACCGGATA ATGGACCTGT TAATCGAAAC ATTGAAGATA 181 TATAAAGGAA GAGGAATCCT GGCATATCAT CAATTGAATA AGTTGAATTA ATTATTTCAA 241 TCTCATTCTC ACTTTCTGAC TTATAGTCGC TTTGTTAAAG CTAGCGTCGA CAGGCCTGGA 301 TCCGGATATC AACAAGTTTG TACAAAAAAG CTGAACGAGA AACGTAAAAT GATATAAATA 361 TCAATATATT AAATTAGATT TTGCATAAAA AACAGACTAC ATAATACTGT AAAACACAAC 421 ATATCCAGTC ACTATGGCGG CCGCATTAGG CACCCCAGGC TTTACACTTT ATGCTTCCGG 481 CTCGTATAAT GTGTGGATTT TGAGTTAGGA TCCGTCGAGA TTTTCAGGAG CTAAGGAAGC 541 TAAAATGGAG AAAAAAATCA CTGGATATAC CACCGTTGAT ATATCCCAAT GGCATCGTAA 601 AGAACATTTT GAGGCATTTC AGTCAGTTGC TCAATGTACC TATAACCAGA CCGTTCAGCT 661 GGATATTACG GCCTTTTTAA AGACCGTAAA GAAAAATAAG CACAAGTTTT ATCCGGCCTT 721 TATTCACATT CTTGCCCGCC TGATGAATGC TCATCCGGAA TTCCGTATGG CAATGAAAGA 781 CGGTGAGCTG GTGATATGGG ATAGTGTTCA CCCTTGTTAC ACCGTTTTCC ATGAGCAAAC 841 TGAAACGTTT TCATCGCTCT GGAGTGAATA CCACGACGAT TTCCGGCAGT TTCTACACAT 901 ATATTCGCAA GATGTGGCGT GTTACGGTGA AAACCTGGCC TATTTCCCTA AAGGGTTTAT 961 TGAGAATATG TTTTTCGTCT CAGCCAATCC CTGGGTGAGT TTCACCAGTT TTGATTTAAA 1021 CGTGGCCAAT ATGGACACTT CTTCGCCCCC GTTTTCACCA TGGGCAAATA TATACGCAGC 1081 GACAGTGCTG ATGCGCTGGC GATCAGTCAT CATGCGGTTG TGATGGCTCC AATGTCCGCC 1141 GATGCTATGA TACAACAGTA CTGCATGATT GCCAGGCCGG GGCGTAAAGG AATCTTG //
Primer: Cm_C_F Sequence file: RDB08940_A5Fgb.seq
>06151_08940_A5Fg_Cm-C_F_E01_13.ab1 1 GGCGGGGACT GGGATCCGGC TTACTAAAAG CCAGATAACA GTATGCGTAT TTGCGCGCTG 61 ATTTTTGCGG TATAAGAATA TATACTGATA TGTATACCCG AAGTATGTCA AAAAGAGGTA 121 TGCTATGAAG CAGCGTATTA CAGTGACAGT TGACAGCGAC AGCTATCAGT TGCTCAAGGC 181 ATATATGATG TCAATATCTC CGGTCTGGTA AGCACAACCA TGCAGAATGA AGCCCGTCGT 241 CTGCGTGCCG AACGCTGGAA AGCGGAAAAT CAGGAAGGGA TGGCTGAGGT CGCCCGGTTT 301 ATTGAAATGA ACGGCTCTTT TGCTGACGAG AACAGGGGCT GGTGAAATGC AGTTTAAGGT 361 TTACACCTAT AAAAGAGAGA GCCGTTATCG TCTGTTTGTG GATGTACAGA GTGATATTAT 421 TGACACGCCC GGGCGACGGA TGGTGATCCC CCTGGCCAGT GCACGTCTGC TGTCAGATAA 481 AGTCTCCCGT GAACTTTACC CGGTGGTGCA TATCGGGGAT GAAAGCTGGC GCATGATGAC 541 CACCGATATG GCCAGTGTGC CGGTCTCCGT TATCGGGGAA GAAGTGGCTG ATCTCAGCCA 601 CCGCGAAAAT GACATCAAAA ACGCCATTAA CCTGATGTTC TGGGGAATAT AAATGTCAGG 661 CTCCCTTATA CACAGCCAGT CTGCAGGTCG ACCATAGTGA CTGGATATGT TGTGTTTTAC 721 AGTATTATGT AGTCTGTTTT TTATGCAAAA TCTAATTTAA TATATTGATA TTTATATCAT 781 TTTACGTTTC TCGTTCAGCT TTCTTGTACA AAGTGGTTGA TATCATGGTG AGCAAGGGCG 841 AGGAGCTGTT CACCGGGGTG GTGCCCATCC TGGTCGAGCT GGACGGCGAC GTAAACGGCC 901 ACAAGTTCAG CGTGTCCGGC GAGGGCGAGG GCGATGCCAC CTACGGCAAG CTGACCCTGA 961 AGTTCATCTG CACCACCGGC AAGCTGCCCG TGCCCTGGCC CACCCTCGTG ACCACCTTCG 1021 GCTACGGCCT GCAGTGCTTC GCCCGCTACC CCGACCACAT GAAGCAGCAC GACTTCTTCA 1081 AGTCGTCATG CCCGAAGCTA CGTCCAGGAG CGCAACAATC TCTCACGAAC GACGCCACCT 1141 ACAGACCCGC GCCCGAAGGG TGAAGTTCCA AAG //
Primer: ADH_rev Sequence file: RDB08940_A5Fgc.seq
>06151_08940_A5Fg_ADH_rev_F01_16.ab1 1 GAGGAGTACT CAGATAAGAT TTTCGTTTTA AAACCTAAGA GTCACTTTAA AATTTGTATA 61 CACTTATTTT TTTTATAACT TATTTAATAA TAAAAATCAT AAATCATAAG AAATTCGCTT 121 ATTTAGAAGT GGCGCGCCGG ATCCTCTAGA GTCGAGATCT CAGTGGTGAT GGTGGTGATG 181 TTTGTCATCG TCGTCCTTGT AGTCCATGGG CTTGTACAGC TCGTCCATGC CGAGAGTGAT 241 CCCGGCGGCG GTCACGAACT CCAGCAGGAC CATGTGATCG CGCTTCTCGT TGGGGTCTTT 301 GCTCAGGGCG GACTGGTAGC TCAGGTAGTG GTTGTCGGGC AGCAGCACGG GGCCGTCGCC 361 GATGGGGGTG TTCTGCTGGT AGTGGTCGGC GAGCTGCACG CTGCCGTCCT CGATGTTGTG 421 GCGGATCTTG AAGTTCACCT TGATGCCGTT CTTCTGCTTG TCGGCCATGA TATAGACGTT 481 GTGGCTGTTG TAGTTGTACT CCAGCTTGTG CCCCAGGATG TTGCCGTCCT CCTTGAAGTC 541 GATGCCCTTC AGCTCGATGC GGTTCACCAG GGTGTCGCCC TCGAACTTCA CCTCGGCGCG 601 GGTCTTGTAG TTGCCGTCGT CCTTGAAGAA GATGGTGCGC TCCTGGACGT AGCCTTCGGG 661 CATGGCGGAC TTGAAGAAGT CGTGCTGCTT CATGTGGTCG GGGTAGCGGG CGAAGCACTG 721 CAGGCCGTAG CCGAAGGTGG TCACGAGGGT GGGCCAGGGC ACGGGCAGCT TGCCGGTGGT 781 GCAGATGAAC TTCAGGGTCA GCTTGCCGTA GGTGGCATCG CCCTCGCCCT CGCCGGACAC 841 GCTGAACTTG TGGCCGTTTA CGTCGCCGTC CAGCTCGACC AGGATGGGCA CCACCCCGGT 901 GAACAGCTCC TCGCCCTTGC TCACCATGAT ATCAACCACT TTGTACAGAA AGCCTGAACG 961 AGAAACGTAA AATGATATAA ATATCAATAT ATTAAATTAG ATTTTGCATA AAAAACAGAC 1021 TACATAATAC TGTAAAACAC AACATATCCA GTCACTATGG GTCGACCTGC AGACTGGCTG 1081 TGTATATGGA GGCTGGACAT TTATATTCCC CAGACCATCA GTATGACGAT TTTTGATGTC 1141 AATTTTCCCC CGGGTGGCCT TGGAGAAATT ACAGGC //
Primer: ADH-Forward Sequence file: RDB08940_A5Fgd.seq
>06151_08940_A5Fg_ADH_Forward_G01_19.ab1 1 ACTCCTTAAT TGACAACACC TCTACCGGCA GATCCGCTAG GGATAACAGG GTAATATACT 61 AGTAAGATGA TTCTTTTATC GTCGATAACG ATGAGGATGT AGACGATTAT GATGAGAGTG 121 ATTAGCTTCT AAAATTTGGT GTTTTCAAAC GCTATTTACA GTACAATCTG AAAAAATTGG 181 ATGAACATTG TAAATGGTAG GATACGAGTT CCAATTAATG TATTTTTTAA GTATTACACT 241 TAGCTTATTA ATTTTATATA TACATTCTAC CTCTGTGGGT TTAAAGACAA CTCGTACACA 301 TACTCAAATT ATTTATGAAC ATTATAATGC ATATTCGTAC TGTCTATTAA ACATTTTTTT 361 GACATTAAAA ATTTCGTTTA CTAACGTAGA AAGCGCTGAT AATTGAGGCT ACTATTTTAA 421 TATCTAGAAA CAAAGATTCA TTAATTAATT AAGTAAGCTC CAGGATACTT GTATATTTCG 481 TTAAAAAAGC GCAATTTCAA CAATTCCTAT GAACATCCAT GATTTCATCA CAAAATCAAA 541 CATATCAGGA TTTAACGTGA ATTATTTGCT TTCTTGTGAT TTTCATCTGA TTTCTGGTCA 601 TTTACGTTAC TGTAAACAAG CTTTTTAAAA AAAGGATTTT TTACGCAATA TTATCGATAT 661 CCCAATCTGT AGTATAAAAA GTGCAACAGT GTTGAGTTTC CCGAAACCAG GCATTTTTGC 721 GGTAGGGTCT GAAACTTTCA CGAAACCATT TCACAACAAA ACTTCTTCAA CAACATGTGT 781 GCAAAGAAAA TCGTCGTCTT ACCAGGAGAC CATATTGGCC CTGAAATTGT TGCTTCTGCC 841 TTGGAGGTTT TGAAAGTCGT TGAGAAGAAG CGACCTGAGT TAAAACTCGA GTTTGAAGAA 901 CACAAGATTG GAGGTGCCTC TATTGATGCC TATGGAACCC CTTTGACTGA CGAGACTGTG 961 AAGGCTTGTT TGGAAGCTGA CGGTGTTCTT TTGGTGCCGT TGGTGGTCCT GATGGACCAC 1021 CCCAATTGTC GTCCTGAGCA GTTTATGAGC TCGTAGGAGT ATGGGTGTTT GGCACTCGAC 1081 TGCCACTTGG CAGCAGTCTT AGTCAGTACA GCCATGAAGC TGGAATCGTT GAGGTTCGAT 1141 ATGGTTGTAC CGGAACTACT GAGCGTGATA CTTGGGGGAG CGTCACAT //
Primer: ars_R1 Sequence file: RDB08940_A5Fge.seq
>06151_08940_A5Fg_ars_R1_H01_22.ab1 1 AGGTTCGTTA TACTAATTAC TTAAGTACCT TTAACTAAAC AAAATGCCTA TATATATATT 61 AATTTACAAT GAGTGTCAGA TAAGTCACTA TGTCCGAGTG GTTAAGGAGT TAGACTCGAA 121 TTCGCAAGCT TAGCTACAAA TCCCACTGGC TATATGTATG CATTTGTGTT AAAAAAGTTT 181 GTATAGATTA TTTAATCTAC TCAGCATTCT TTCTCTAAAT AGGAATTTGT TACTTAATGG 241 AGAAAAAAAT GTTTCGATTT ACCTAGTGTA TTTGTTTGTA TACTCACGTT TAATTTCAAA 301 CATCCATTCT ATCTTGTGTA ATTTTTGGCA TGGTGAAAAA GATAATCAGC CTTATAATCT 361 TTACAAAAGT AAGAAATTCT GTAAATAAGC CTTAATGCCC TTGCTTTAAA TTAAAATGGT 421 TCTTTTTCAT GATAATGTTT GCACTTTGTG AATATATTTT AGATAGTTCT GTGAGGTATA 481 ATTAAGATGT TTTAGAGACT TATACAATTT TGTCTTTATA AATTCTTAAT TGATTTTACC 541 ATCCCAGTTT AACTATGCTT CGTCGGCATC TCTGCACATG TCGTGTTTTC TTACCGTATT 601 GTCCTACCAA GAACCTCTTT TTTGCTTGGA TCGAAATTAA AGGTTTAAAA GCAAAGTTAT 661 GGATGCTAGA GTATTTCAAA GCTATTCAGC TAGAGCTGAG GGGATGAAAA ATCCCATTGC 721 CAAGGAATTG TTGGCTTTGA TGGAAGAAAA GCAAAGCAAC TTGTCAGTCG CGGTCGATTT 781 GACGAAGAAA TCCGAAATCT TAGAATTGGT AGATAAAATT GGACCCTATG TCTGTGTTAT 841 CAAGACACAT ATTGACGTTG TCGAGGATTT CGACCAGGAT ATGGTAGAAA AACTGGTGGC 901 CTTAGGTAAA AAGCATCGTT TTCTTATCTT TGAGGATCGC AAATTCGCAG ACATTGGAAA 961 TACCGTCAAG CTACAATATG CATCTGGTGT GTACAAAATT GCTTCTTGGG CTCATATCAC 1021 AAATTGCCAT ACAGTGCCAG GCGAGGGTAT ATACACGCTC AAAGAGTGGT TTACCTTTGG 1081 ACGTGTCTCT GCTTTGCTGA AATGTCTCAA AGCTCTTTGC TACTGATCTA CAAGAGAATC 1141 TAGATTGATG GAAGCCAACG ATTTTGCTTG GCTTATAGCT GCTCGTCGAA TTCCTACGTT 1201 CCAAGGCGAC CATCAC //
Primer: f1ori_R3 Sequence file: RDB08940_A5Fgf.seq
>06151_08940_A5Fg_M13(-40)_A02_02.ab1 1 AGGCCAGAGA TTCGAGCTCG GGAGCGCTAC CGTGAATGGG CTCAACCAAA CAATGAACCT 61 TTTCTTCTGA TTTTCCTACC ACACCGGAAA GGGAGGCAGA AGGCAAAAGG CCCAAGCTAC 121 CAGGAATGAC AGAAGCCTCA TCTGAAATAA TGTCACCAAA CAAGTTGTCA GTCAAAACAA 181 CACCGTTAAG TGTACGAGGG CTCTTGACCA AAAGCATGGC TGCGGAGTCA ATGAGCTGGT 241 TTTTTAAGGT AAGGTGAGGA TATTCCTCCT TAAAAATCTT AGCTACAGTC TTGCGCCAAA 301 GACGAGAAGT TGCCAAAACA TTAGCTTTGT CGAGTAATGT GACGGGAGCA GGAGGGTTGG 361 AAGTTTCAGC TAAGCGGCCG CGGGCCCAAC CTTCCAATTC ATTAAATCGA GTCCTCCATC 421 AGATGATTTA CAAGGTTTTG CATAGAATCC CTAAGTAAGA GAAGTTGTTA GTGATTTCTT 481 CAAGTTTACT AAAATTTACA TACAAATGGA TGATCTCTTC TCCATTGTTT CCTTTCTTCT 541 TGCAAACGCG TTTTACAAAG AGATGACATG CTATTTTCTG ATTATTTTTT TCTATAGTTT 601 TCTATTTTGT ACGTCCTTGG GAGTCCGAAA TGTAAAATCG GGGTATGGTT AGTAGCGGCA 661 GCGTTCATTA TGGTATATAA AAGATAAAAA GTAGGGGCAA GCGAGACAGA ATGGGATACA 721 AGGGCATCGT CTATAATTAT AGCTAAAAAT TGTATTTTAA TTTGTATTTT TTGTAATTTT 781 ATTTCTCATT GTTTTACTTA AAATGAATGC GAATTAGAGA AAACTTCAAC GAAATGTCAA 841 AATAAGCTCA GCAAATATAC AATTTTAGGG AAAGCGATCA GCAACTTATT CTCCGTTGTA 901 GATTCTTTTT CTTCATAAAT TCCAACAATG TAACTGCCAA TTGATTTTTC TCTAGATGCT 961 AATAAATTGA AACTAATGAA TATAATAACA CAGGTTTTGA TAAATTTGTA AACTCTTACT 1021 CAAAAAAAGA ATGTTAAAAC TAATTTTAAC CCTTTTTATA ACGGCTCATG GCGTTAAAGA 1081 GATAGTAAAA TCTACATTTT TTTATTTAAT GTCACACTGT CTGCGAATAT ATCCTAATAT 1141 TTTCGCTTAC TGCTGTTTTT AGTACCTATT TAACCTTTCA GA //

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References

Original, user report and related articles

user_report	Eckler, A.M., Haploinsufficiency of the Sec7 guanine nucleotide exchange factor gea1 impairs septation in fission yeast. PLoS One, 8 (2): e56807 (2013). PMID 23457617. [link to RRC of NBRP]
user_report	Stigliano I.D., Glucosidase II beta subunit modulates N-glycan trimming in fission yeasts and mammals. Mol. Biol. Cell, 20, 3974-3984 (2009). PMID 19605557. [link to RRC of NBRP]

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