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pDUAL-YFH1c

S. pombe 3 ter end YFP-FLAG-His6-tagged vector with nmt1 promoter

Catalog number RDB06151
Resource name pDUAL-YFH1c
Clone info. S. pombe 3 ter end YFP-FLAG-His6-tagged vector with nmt1 promoter. Multicopy marker, ura4+; Integration marker, leu1+.
Comment Gateway compatible.
Vector backbone pDUAL (Plasmid)
Selectable markers E. coli: Amp^r, Cm^r, ccdB.
Growth remarks Use DB3.1, ccdB Survival or equivalent to amplify this clone. 30 degC is fine.
Reference of insert sequence AB364104.1 (DDBJ accession number)
Depositor|Developer Yoshida, Minoru | Matsuyama, Akihisa |
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External Database

            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature(s). In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must state an acknowledgment to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR . When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
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            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
            Remarks Use Survival2 (Invitrogen) or equivalent to amplify this clone.
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            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献の引用と、寄託者への謝辞の表明を必要とする。 利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。 寄託者から要請がある場合、理研BRCは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
            GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします (Schedule AとSchedule Bを入手[link])。
            備考 このクローンの増殖にはSurvival2 (Invitrogen) あるいは同等の宿主を使用してください。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB06151 pDUAL-YFH1c DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Sequence RDB06151z.seq

            Original reference

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Dnaconda's recommendation EXR0013j (Japanese text)
            Featured content Dnaconda's recommendation EXR0025j (Japanese text)
            Featured content Expression Vector Backbone of Schizosaccharomyces pombe (English text)

            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB08940_A5Fgp1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: nmt1Fw
            Sequence file: RDB08940_A5Fga.seq check
            >06151_08940_A5Fg_nmt1Fw_D01_10.ab1
                1 GGAATTTTAT TAATCAGGGA AAACGTAACT CTCGGCTACT GGATGGTTCA GTCACCCAAC
               61 GATTACTGGG GAGAGAAAAC AGGGCAAAAG CAAAGCTTAA AGGAATCCGA TTGTCATTCG
              121 GCAATGTGCA GCGAAACTAA AAACCGGATA ATGGACCTGT TAATCGAAAC ATTGAAGATA
              181 TATAAAGGAA GAGGAATCCT GGCATATCAT CAATTGAATA AGTTGAATTA ATTATTTCAA
              241 TCTCATTCTC ACTTTCTGAC TTATAGTCGC TTTGTTAAAG CTAGCGTCGA CAGGCCTGGA
              301 TCCGGATATC AACAAGTTTG TACAAAAAAG CTGAACGAGA AACGTAAAAT GATATAAATA
              361 TCAATATATT AAATTAGATT TTGCATAAAA AACAGACTAC ATAATACTGT AAAACACAAC
              421 ATATCCAGTC ACTATGGCGG CCGCATTAGG CACCCCAGGC TTTACACTTT ATGCTTCCGG
              481 CTCGTATAAT GTGTGGATTT TGAGTTAGGA TCCGTCGAGA TTTTCAGGAG CTAAGGAAGC
              541 TAAAATGGAG AAAAAAATCA CTGGATATAC CACCGTTGAT ATATCCCAAT GGCATCGTAA
              601 AGAACATTTT GAGGCATTTC AGTCAGTTGC TCAATGTACC TATAACCAGA CCGTTCAGCT
              661 GGATATTACG GCCTTTTTAA AGACCGTAAA GAAAAATAAG CACAAGTTTT ATCCGGCCTT
              721 TATTCACATT CTTGCCCGCC TGATGAATGC TCATCCGGAA TTCCGTATGG CAATGAAAGA
              781 CGGTGAGCTG GTGATATGGG ATAGTGTTCA CCCTTGTTAC ACCGTTTTCC ATGAGCAAAC
              841 TGAAACGTTT TCATCGCTCT GGAGTGAATA CCACGACGAT TTCCGGCAGT TTCTACACAT
              901 ATATTCGCAA GATGTGGCGT GTTACGGTGA AAACCTGGCC TATTTCCCTA AAGGGTTTAT
              961 TGAGAATATG TTTTTCGTCT CAGCCAATCC CTGGGTGAGT TTCACCAGTT TTGATTTAAA
             1021 CGTGGCCAAT ATGGACACTT CTTCGCCCCC GTTTTCACCA TGGGCAAATA TATACGCAGC
             1081 GACAGTGCTG ATGCGCTGGC GATCAGTCAT CATGCGGTTG TGATGGCTCC AATGTCCGCC
            1141 GATGCTATGA TACAACAGTA CTGCATGATT GCCAGGCCGG GGCGTAAAGG AATCTTG
            //
            Primer: Cm_C_F
            Sequence file: RDB08940_A5Fgb.seq check
            >06151_08940_A5Fg_Cm-C_F_E01_13.ab1
                1 GGCGGGGACT GGGATCCGGC TTACTAAAAG CCAGATAACA GTATGCGTAT TTGCGCGCTG
               61 ATTTTTGCGG TATAAGAATA TATACTGATA TGTATACCCG AAGTATGTCA AAAAGAGGTA
              121 TGCTATGAAG CAGCGTATTA CAGTGACAGT TGACAGCGAC AGCTATCAGT TGCTCAAGGC
              181 ATATATGATG TCAATATCTC CGGTCTGGTA AGCACAACCA TGCAGAATGA AGCCCGTCGT
              241 CTGCGTGCCG AACGCTGGAA AGCGGAAAAT CAGGAAGGGA TGGCTGAGGT CGCCCGGTTT
              301 ATTGAAATGA ACGGCTCTTT TGCTGACGAG AACAGGGGCT GGTGAAATGC AGTTTAAGGT
              361 TTACACCTAT AAAAGAGAGA GCCGTTATCG TCTGTTTGTG GATGTACAGA GTGATATTAT
              421 TGACACGCCC GGGCGACGGA TGGTGATCCC CCTGGCCAGT GCACGTCTGC TGTCAGATAA
              481 AGTCTCCCGT GAACTTTACC CGGTGGTGCA TATCGGGGAT GAAAGCTGGC GCATGATGAC
              541 CACCGATATG GCCAGTGTGC CGGTCTCCGT TATCGGGGAA GAAGTGGCTG ATCTCAGCCA
              601 CCGCGAAAAT GACATCAAAA ACGCCATTAA CCTGATGTTC TGGGGAATAT AAATGTCAGG
              661 CTCCCTTATA CACAGCCAGT CTGCAGGTCG ACCATAGTGA CTGGATATGT TGTGTTTTAC
              721 AGTATTATGT AGTCTGTTTT TTATGCAAAA TCTAATTTAA TATATTGATA TTTATATCAT
              781 TTTACGTTTC TCGTTCAGCT TTCTTGTACA AAGTGGTTGA TATCATGGTG AGCAAGGGCG
              841 AGGAGCTGTT CACCGGGGTG GTGCCCATCC TGGTCGAGCT GGACGGCGAC GTAAACGGCC
              901 ACAAGTTCAG CGTGTCCGGC GAGGGCGAGG GCGATGCCAC CTACGGCAAG CTGACCCTGA
              961 AGTTCATCTG CACCACCGGC AAGCTGCCCG TGCCCTGGCC CACCCTCGTG ACCACCTTCG
             1021 GCTACGGCCT GCAGTGCTTC GCCCGCTACC CCGACCACAT GAAGCAGCAC GACTTCTTCA
             1081 AGTCGTCATG CCCGAAGCTA CGTCCAGGAG CGCAACAATC TCTCACGAAC GACGCCACCT
            1141 ACAGACCCGC GCCCGAAGGG TGAAGTTCCA AAG
            //
            Primer: ADH_rev
            Sequence file: RDB08940_A5Fgc.seq check
            >06151_08940_A5Fg_ADH_rev_F01_16.ab1
                1 GAGGAGTACT CAGATAAGAT TTTCGTTTTA AAACCTAAGA GTCACTTTAA AATTTGTATA
               61 CACTTATTTT TTTTATAACT TATTTAATAA TAAAAATCAT AAATCATAAG AAATTCGCTT
              121 ATTTAGAAGT GGCGCGCCGG ATCCTCTAGA GTCGAGATCT CAGTGGTGAT GGTGGTGATG
              181 TTTGTCATCG TCGTCCTTGT AGTCCATGGG CTTGTACAGC TCGTCCATGC CGAGAGTGAT
              241 CCCGGCGGCG GTCACGAACT CCAGCAGGAC CATGTGATCG CGCTTCTCGT TGGGGTCTTT
              301 GCTCAGGGCG GACTGGTAGC TCAGGTAGTG GTTGTCGGGC AGCAGCACGG GGCCGTCGCC
              361 GATGGGGGTG TTCTGCTGGT AGTGGTCGGC GAGCTGCACG CTGCCGTCCT CGATGTTGTG
              421 GCGGATCTTG AAGTTCACCT TGATGCCGTT CTTCTGCTTG TCGGCCATGA TATAGACGTT
              481 GTGGCTGTTG TAGTTGTACT CCAGCTTGTG CCCCAGGATG TTGCCGTCCT CCTTGAAGTC
              541 GATGCCCTTC AGCTCGATGC GGTTCACCAG GGTGTCGCCC TCGAACTTCA CCTCGGCGCG
              601 GGTCTTGTAG TTGCCGTCGT CCTTGAAGAA GATGGTGCGC TCCTGGACGT AGCCTTCGGG
              661 CATGGCGGAC TTGAAGAAGT CGTGCTGCTT CATGTGGTCG GGGTAGCGGG CGAAGCACTG
              721 CAGGCCGTAG CCGAAGGTGG TCACGAGGGT GGGCCAGGGC ACGGGCAGCT TGCCGGTGGT
              781 GCAGATGAAC TTCAGGGTCA GCTTGCCGTA GGTGGCATCG CCCTCGCCCT CGCCGGACAC
              841 GCTGAACTTG TGGCCGTTTA CGTCGCCGTC CAGCTCGACC AGGATGGGCA CCACCCCGGT
              901 GAACAGCTCC TCGCCCTTGC TCACCATGAT ATCAACCACT TTGTACAGAA AGCCTGAACG
              961 AGAAACGTAA AATGATATAA ATATCAATAT ATTAAATTAG ATTTTGCATA AAAAACAGAC
             1021 TACATAATAC TGTAAAACAC AACATATCCA GTCACTATGG GTCGACCTGC AGACTGGCTG
             1081 TGTATATGGA GGCTGGACAT TTATATTCCC CAGACCATCA GTATGACGAT TTTTGATGTC
            1141 AATTTTCCCC CGGGTGGCCT TGGAGAAATT ACAGGC
            //
            Primer: ADH-Forward
            Sequence file: RDB08940_A5Fgd.seq check
            >06151_08940_A5Fg_ADH_Forward_G01_19.ab1
                1 ACTCCTTAAT TGACAACACC TCTACCGGCA GATCCGCTAG GGATAACAGG GTAATATACT
               61 AGTAAGATGA TTCTTTTATC GTCGATAACG ATGAGGATGT AGACGATTAT GATGAGAGTG
              121 ATTAGCTTCT AAAATTTGGT GTTTTCAAAC GCTATTTACA GTACAATCTG AAAAAATTGG
              181 ATGAACATTG TAAATGGTAG GATACGAGTT CCAATTAATG TATTTTTTAA GTATTACACT
              241 TAGCTTATTA ATTTTATATA TACATTCTAC CTCTGTGGGT TTAAAGACAA CTCGTACACA
              301 TACTCAAATT ATTTATGAAC ATTATAATGC ATATTCGTAC TGTCTATTAA ACATTTTTTT
              361 GACATTAAAA ATTTCGTTTA CTAACGTAGA AAGCGCTGAT AATTGAGGCT ACTATTTTAA
              421 TATCTAGAAA CAAAGATTCA TTAATTAATT AAGTAAGCTC CAGGATACTT GTATATTTCG
              481 TTAAAAAAGC GCAATTTCAA CAATTCCTAT GAACATCCAT GATTTCATCA CAAAATCAAA
              541 CATATCAGGA TTTAACGTGA ATTATTTGCT TTCTTGTGAT TTTCATCTGA TTTCTGGTCA
              601 TTTACGTTAC TGTAAACAAG CTTTTTAAAA AAAGGATTTT TTACGCAATA TTATCGATAT
              661 CCCAATCTGT AGTATAAAAA GTGCAACAGT GTTGAGTTTC CCGAAACCAG GCATTTTTGC
              721 GGTAGGGTCT GAAACTTTCA CGAAACCATT TCACAACAAA ACTTCTTCAA CAACATGTGT
              781 GCAAAGAAAA TCGTCGTCTT ACCAGGAGAC CATATTGGCC CTGAAATTGT TGCTTCTGCC
              841 TTGGAGGTTT TGAAAGTCGT TGAGAAGAAG CGACCTGAGT TAAAACTCGA GTTTGAAGAA
              901 CACAAGATTG GAGGTGCCTC TATTGATGCC TATGGAACCC CTTTGACTGA CGAGACTGTG
              961 AAGGCTTGTT TGGAAGCTGA CGGTGTTCTT TTGGTGCCGT TGGTGGTCCT GATGGACCAC
             1021 CCCAATTGTC GTCCTGAGCA GTTTATGAGC TCGTAGGAGT ATGGGTGTTT GGCACTCGAC
             1081 TGCCACTTGG CAGCAGTCTT AGTCAGTACA GCCATGAAGC TGGAATCGTT GAGGTTCGAT
            1141 ATGGTTGTAC CGGAACTACT GAGCGTGATA CTTGGGGGAG CGTCACAT
            //
            Primer: ars_R1
            Sequence file: RDB08940_A5Fge.seq check
            >06151_08940_A5Fg_ars_R1_H01_22.ab1
                1 AGGTTCGTTA TACTAATTAC TTAAGTACCT TTAACTAAAC AAAATGCCTA TATATATATT
               61 AATTTACAAT GAGTGTCAGA TAAGTCACTA TGTCCGAGTG GTTAAGGAGT TAGACTCGAA
              121 TTCGCAAGCT TAGCTACAAA TCCCACTGGC TATATGTATG CATTTGTGTT AAAAAAGTTT
              181 GTATAGATTA TTTAATCTAC TCAGCATTCT TTCTCTAAAT AGGAATTTGT TACTTAATGG
              241 AGAAAAAAAT GTTTCGATTT ACCTAGTGTA TTTGTTTGTA TACTCACGTT TAATTTCAAA
              301 CATCCATTCT ATCTTGTGTA ATTTTTGGCA TGGTGAAAAA GATAATCAGC CTTATAATCT
              361 TTACAAAAGT AAGAAATTCT GTAAATAAGC CTTAATGCCC TTGCTTTAAA TTAAAATGGT
              421 TCTTTTTCAT GATAATGTTT GCACTTTGTG AATATATTTT AGATAGTTCT GTGAGGTATA
              481 ATTAAGATGT TTTAGAGACT TATACAATTT TGTCTTTATA AATTCTTAAT TGATTTTACC
              541 ATCCCAGTTT AACTATGCTT CGTCGGCATC TCTGCACATG TCGTGTTTTC TTACCGTATT
              601 GTCCTACCAA GAACCTCTTT TTTGCTTGGA TCGAAATTAA AGGTTTAAAA GCAAAGTTAT
              661 GGATGCTAGA GTATTTCAAA GCTATTCAGC TAGAGCTGAG GGGATGAAAA ATCCCATTGC
              721 CAAGGAATTG TTGGCTTTGA TGGAAGAAAA GCAAAGCAAC TTGTCAGTCG CGGTCGATTT
              781 GACGAAGAAA TCCGAAATCT TAGAATTGGT AGATAAAATT GGACCCTATG TCTGTGTTAT
              841 CAAGACACAT ATTGACGTTG TCGAGGATTT CGACCAGGAT ATGGTAGAAA AACTGGTGGC
              901 CTTAGGTAAA AAGCATCGTT TTCTTATCTT TGAGGATCGC AAATTCGCAG ACATTGGAAA
              961 TACCGTCAAG CTACAATATG CATCTGGTGT GTACAAAATT GCTTCTTGGG CTCATATCAC
             1021 AAATTGCCAT ACAGTGCCAG GCGAGGGTAT ATACACGCTC AAAGAGTGGT TTACCTTTGG
             1081 ACGTGTCTCT GCTTTGCTGA AATGTCTCAA AGCTCTTTGC TACTGATCTA CAAGAGAATC
             1141 TAGATTGATG GAAGCCAACG ATTTTGCTTG GCTTATAGCT GCTCGTCGAA TTCCTACGTT
            1201 CCAAGGCGAC CATCAC
            //
            Primer: f1ori_R3
            Sequence file: RDB08940_A5Fgf.seq check
            >06151_08940_A5Fg_M13(-40)_A02_02.ab1
                1 AGGCCAGAGA TTCGAGCTCG GGAGCGCTAC CGTGAATGGG CTCAACCAAA CAATGAACCT
               61 TTTCTTCTGA TTTTCCTACC ACACCGGAAA GGGAGGCAGA AGGCAAAAGG CCCAAGCTAC
              121 CAGGAATGAC AGAAGCCTCA TCTGAAATAA TGTCACCAAA CAAGTTGTCA GTCAAAACAA
              181 CACCGTTAAG TGTACGAGGG CTCTTGACCA AAAGCATGGC TGCGGAGTCA ATGAGCTGGT
              241 TTTTTAAGGT AAGGTGAGGA TATTCCTCCT TAAAAATCTT AGCTACAGTC TTGCGCCAAA
              301 GACGAGAAGT TGCCAAAACA TTAGCTTTGT CGAGTAATGT GACGGGAGCA GGAGGGTTGG
              361 AAGTTTCAGC TAAGCGGCCG CGGGCCCAAC CTTCCAATTC ATTAAATCGA GTCCTCCATC
              421 AGATGATTTA CAAGGTTTTG CATAGAATCC CTAAGTAAGA GAAGTTGTTA GTGATTTCTT
              481 CAAGTTTACT AAAATTTACA TACAAATGGA TGATCTCTTC TCCATTGTTT CCTTTCTTCT
              541 TGCAAACGCG TTTTACAAAG AGATGACATG CTATTTTCTG ATTATTTTTT TCTATAGTTT
              601 TCTATTTTGT ACGTCCTTGG GAGTCCGAAA TGTAAAATCG GGGTATGGTT AGTAGCGGCA
              661 GCGTTCATTA TGGTATATAA AAGATAAAAA GTAGGGGCAA GCGAGACAGA ATGGGATACA
              721 AGGGCATCGT CTATAATTAT AGCTAAAAAT TGTATTTTAA TTTGTATTTT TTGTAATTTT
              781 ATTTCTCATT GTTTTACTTA AAATGAATGC GAATTAGAGA AAACTTCAAC GAAATGTCAA
              841 AATAAGCTCA GCAAATATAC AATTTTAGGG AAAGCGATCA GCAACTTATT CTCCGTTGTA
              901 GATTCTTTTT CTTCATAAAT TCCAACAATG TAACTGCCAA TTGATTTTTC TCTAGATGCT
              961 AATAAATTGA AACTAATGAA TATAATAACA CAGGTTTTGA TAAATTTGTA AACTCTTACT
             1021 CAAAAAAAGA ATGTTAAAAC TAATTTTAAC CCTTTTTATA ACGGCTCATG GCGTTAAAGA
             1081 GATAGTAAAA TCTACATTTT TTTATTTAAT GTCACACTGT CTGCGAATAT ATCCTAATAT
            1141 TTTCGCTTAC TGCTGTTTTT AGTACCTATT TAACCTTTCA GA
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            user_report Eckler, A.M., Haploinsufficiency of the Sec7 guanine nucleotide exchange factor gea1 impairs septation in fission yeast. PLoS One, 8 (2): e56807 (2013). PMID 23457617.
            user_report Stigliano I.D., Glucosidase II beta subunit modulates N-glycan trimming in fission yeasts and mammals. Mol. Biol. Cell, 20, 3974-3984 (2009). PMID 19605557.

            2022.05.18

            GNP_filter3_RDBDEP_html_220516.pl