Resource data sheet
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pDUAL-YFH1c

S. pombe 3 ter end YFP-FLAG-His6-tagged vector with nmt1 promoter

Catalog number RDB06151
Resource name pDUAL-YFH1c
Clone info. S. pombe 3 ter end YFP-FLAG-His6-tagged vector with nmt1 promoter.
Multicopy marker, ura4+; Integration marker, leu1+. Gateway compatible.
Vector backbone pDUAL (Plasmid)
Selectable markers Amp^r, Cm^r
Reference of nucleotide sequence AB364104.1 (DDBJ accession number)
Depositor Yoshida, Minoru | Matsuyama, Akihisa |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature(s). In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must state an acknowledgment to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR . When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
Ordering Please visit Information of Request for Distribution. 
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Material Transfer Agreement (MTA) [Word]
Schedule A (GFP-A) [Link]
Remarks Use Survival2 (Invitrogen) or equivalent to amplify this clone.
Please use Schedule A (GFP-A) additionally.
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB06151 pDUAL-YFH1c DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB06151z.seq from Depositor

Featured content

Featured content Dnaconda's recommendation EXR0013j (Japanese text)
Featured content Dnaconda's recommendation EXR0025j (Japanese text)
Featured content Expression Vector Backbone of Schizosaccharomyces pombe (English text)

References

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB08940_A5Fgp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: nmt1Fw RDB08940_A5Fga.seq
>06151_08940_A5Fg_nmt1Fw_D01_10.ab1
    1 GGAATTTTAT TAATCAGGGA AAACGTAACT CTCGGCTACT GGATGGTTCA GTCACCCAAC
   61 GATTACTGGG GAGAGAAAAC AGGGCAAAAG CAAAGCTTAA AGGAATCCGA TTGTCATTCG
  121 GCAATGTGCA GCGAAACTAA AAACCGGATA ATGGACCTGT TAATCGAAAC ATTGAAGATA
  181 TATAAAGGAA GAGGAATCCT GGCATATCAT CAATTGAATA AGTTGAATTA ATTATTTCAA
  241 TCTCATTCTC ACTTTCTGAC TTATAGTCGC TTTGTTAAAG CTAGCGTCGA CAGGCCTGGA
  301 TCCGGATATC AACAAGTTTG TACAAAAAAG CTGAACGAGA AACGTAAAAT GATATAAATA
  361 TCAATATATT AAATTAGATT TTGCATAAAA AACAGACTAC ATAATACTGT AAAACACAAC
  421 ATATCCAGTC ACTATGGCGG CCGCATTAGG CACCCCAGGC TTTACACTTT ATGCTTCCGG
  481 CTCGTATAAT GTGTGGATTT TGAGTTAGGA TCCGTCGAGA TTTTCAGGAG CTAAGGAAGC
  541 TAAAATGGAG AAAAAAATCA CTGGATATAC CACCGTTGAT ATATCCCAAT GGCATCGTAA
  601 AGAACATTTT GAGGCATTTC AGTCAGTTGC TCAATGTACC TATAACCAGA CCGTTCAGCT
  661 GGATATTACG GCCTTTTTAA AGACCGTAAA GAAAAATAAG CACAAGTTTT ATCCGGCCTT
  721 TATTCACATT CTTGCCCGCC TGATGAATGC TCATCCGGAA TTCCGTATGG CAATGAAAGA
  781 CGGTGAGCTG GTGATATGGG ATAGTGTTCA CCCTTGTTAC ACCGTTTTCC ATGAGCAAAC
  841 TGAAACGTTT TCATCGCTCT GGAGTGAATA CCACGACGAT TTCCGGCAGT TTCTACACAT
  901 ATATTCGCAA GATGTGGCGT GTTACGGTGA AAACCTGGCC TATTTCCCTA AAGGGTTTAT
  961 TGAGAATATG TTTTTCGTCT CAGCCAATCC CTGGGTGAGT TTCACCAGTT TTGATTTAAA
 1021 CGTGGCCAAT ATGGACACTT CTTCGCCCCC GTTTTCACCA TGGGCAAATA TATACGCAGC
 1081 GACAGTGCTG ATGCGCTGGC GATCAGTCAT CATGCGGTTG TGATGGCTCC AATGTCCGCC
1141 GATGCTATGA TACAACAGTA CTGCATGATT GCCAGGCCGG GGCGTAAAGG AATCTTG
//
Primer: Cm_C_F RDB08940_A5Fgb.seq
>06151_08940_A5Fg_Cm-C_F_E01_13.ab1
    1 GGCGGGGACT GGGATCCGGC TTACTAAAAG CCAGATAACA GTATGCGTAT TTGCGCGCTG
   61 ATTTTTGCGG TATAAGAATA TATACTGATA TGTATACCCG AAGTATGTCA AAAAGAGGTA
  121 TGCTATGAAG CAGCGTATTA CAGTGACAGT TGACAGCGAC AGCTATCAGT TGCTCAAGGC
  181 ATATATGATG TCAATATCTC CGGTCTGGTA AGCACAACCA TGCAGAATGA AGCCCGTCGT
  241 CTGCGTGCCG AACGCTGGAA AGCGGAAAAT CAGGAAGGGA TGGCTGAGGT CGCCCGGTTT
  301 ATTGAAATGA ACGGCTCTTT TGCTGACGAG AACAGGGGCT GGTGAAATGC AGTTTAAGGT
  361 TTACACCTAT AAAAGAGAGA GCCGTTATCG TCTGTTTGTG GATGTACAGA GTGATATTAT
  421 TGACACGCCC GGGCGACGGA TGGTGATCCC CCTGGCCAGT GCACGTCTGC TGTCAGATAA
  481 AGTCTCCCGT GAACTTTACC CGGTGGTGCA TATCGGGGAT GAAAGCTGGC GCATGATGAC
  541 CACCGATATG GCCAGTGTGC CGGTCTCCGT TATCGGGGAA GAAGTGGCTG ATCTCAGCCA
  601 CCGCGAAAAT GACATCAAAA ACGCCATTAA CCTGATGTTC TGGGGAATAT AAATGTCAGG
  661 CTCCCTTATA CACAGCCAGT CTGCAGGTCG ACCATAGTGA CTGGATATGT TGTGTTTTAC
  721 AGTATTATGT AGTCTGTTTT TTATGCAAAA TCTAATTTAA TATATTGATA TTTATATCAT
  781 TTTACGTTTC TCGTTCAGCT TTCTTGTACA AAGTGGTTGA TATCATGGTG AGCAAGGGCG
  841 AGGAGCTGTT CACCGGGGTG GTGCCCATCC TGGTCGAGCT GGACGGCGAC GTAAACGGCC
  901 ACAAGTTCAG CGTGTCCGGC GAGGGCGAGG GCGATGCCAC CTACGGCAAG CTGACCCTGA
  961 AGTTCATCTG CACCACCGGC AAGCTGCCCG TGCCCTGGCC CACCCTCGTG ACCACCTTCG
 1021 GCTACGGCCT GCAGTGCTTC GCCCGCTACC CCGACCACAT GAAGCAGCAC GACTTCTTCA
 1081 AGTCGTCATG CCCGAAGCTA CGTCCAGGAG CGCAACAATC TCTCACGAAC GACGCCACCT
1141 ACAGACCCGC GCCCGAAGGG TGAAGTTCCA AAG
//
Primer: ADH_rev RDB08940_A5Fgc.seq
>06151_08940_A5Fg_ADH_rev_F01_16.ab1
    1 GAGGAGTACT CAGATAAGAT TTTCGTTTTA AAACCTAAGA GTCACTTTAA AATTTGTATA
   61 CACTTATTTT TTTTATAACT TATTTAATAA TAAAAATCAT AAATCATAAG AAATTCGCTT
  121 ATTTAGAAGT GGCGCGCCGG ATCCTCTAGA GTCGAGATCT CAGTGGTGAT GGTGGTGATG
  181 TTTGTCATCG TCGTCCTTGT AGTCCATGGG CTTGTACAGC TCGTCCATGC CGAGAGTGAT
  241 CCCGGCGGCG GTCACGAACT CCAGCAGGAC CATGTGATCG CGCTTCTCGT TGGGGTCTTT
  301 GCTCAGGGCG GACTGGTAGC TCAGGTAGTG GTTGTCGGGC AGCAGCACGG GGCCGTCGCC
  361 GATGGGGGTG TTCTGCTGGT AGTGGTCGGC GAGCTGCACG CTGCCGTCCT CGATGTTGTG
  421 GCGGATCTTG AAGTTCACCT TGATGCCGTT CTTCTGCTTG TCGGCCATGA TATAGACGTT
  481 GTGGCTGTTG TAGTTGTACT CCAGCTTGTG CCCCAGGATG TTGCCGTCCT CCTTGAAGTC
  541 GATGCCCTTC AGCTCGATGC GGTTCACCAG GGTGTCGCCC TCGAACTTCA CCTCGGCGCG
  601 GGTCTTGTAG TTGCCGTCGT CCTTGAAGAA GATGGTGCGC TCCTGGACGT AGCCTTCGGG
  661 CATGGCGGAC TTGAAGAAGT CGTGCTGCTT CATGTGGTCG GGGTAGCGGG CGAAGCACTG
  721 CAGGCCGTAG CCGAAGGTGG TCACGAGGGT GGGCCAGGGC ACGGGCAGCT TGCCGGTGGT
  781 GCAGATGAAC TTCAGGGTCA GCTTGCCGTA GGTGGCATCG CCCTCGCCCT CGCCGGACAC
  841 GCTGAACTTG TGGCCGTTTA CGTCGCCGTC CAGCTCGACC AGGATGGGCA CCACCCCGGT
  901 GAACAGCTCC TCGCCCTTGC TCACCATGAT ATCAACCACT TTGTACAGAA AGCCTGAACG
  961 AGAAACGTAA AATGATATAA ATATCAATAT ATTAAATTAG ATTTTGCATA AAAAACAGAC
 1021 TACATAATAC TGTAAAACAC AACATATCCA GTCACTATGG GTCGACCTGC AGACTGGCTG
 1081 TGTATATGGA GGCTGGACAT TTATATTCCC CAGACCATCA GTATGACGAT TTTTGATGTC
1141 AATTTTCCCC CGGGTGGCCT TGGAGAAATT ACAGGC
//
Primer: ADH-Forward RDB08940_A5Fgd.seq
>06151_08940_A5Fg_ADH_Forward_G01_19.ab1
    1 ACTCCTTAAT TGACAACACC TCTACCGGCA GATCCGCTAG GGATAACAGG GTAATATACT
   61 AGTAAGATGA TTCTTTTATC GTCGATAACG ATGAGGATGT AGACGATTAT GATGAGAGTG
  121 ATTAGCTTCT AAAATTTGGT GTTTTCAAAC GCTATTTACA GTACAATCTG AAAAAATTGG
  181 ATGAACATTG TAAATGGTAG GATACGAGTT CCAATTAATG TATTTTTTAA GTATTACACT
  241 TAGCTTATTA ATTTTATATA TACATTCTAC CTCTGTGGGT TTAAAGACAA CTCGTACACA
  301 TACTCAAATT ATTTATGAAC ATTATAATGC ATATTCGTAC TGTCTATTAA ACATTTTTTT
  361 GACATTAAAA ATTTCGTTTA CTAACGTAGA AAGCGCTGAT AATTGAGGCT ACTATTTTAA
  421 TATCTAGAAA CAAAGATTCA TTAATTAATT AAGTAAGCTC CAGGATACTT GTATATTTCG
  481 TTAAAAAAGC GCAATTTCAA CAATTCCTAT GAACATCCAT GATTTCATCA CAAAATCAAA
  541 CATATCAGGA TTTAACGTGA ATTATTTGCT TTCTTGTGAT TTTCATCTGA TTTCTGGTCA
  601 TTTACGTTAC TGTAAACAAG CTTTTTAAAA AAAGGATTTT TTACGCAATA TTATCGATAT
  661 CCCAATCTGT AGTATAAAAA GTGCAACAGT GTTGAGTTTC CCGAAACCAG GCATTTTTGC
  721 GGTAGGGTCT GAAACTTTCA CGAAACCATT TCACAACAAA ACTTCTTCAA CAACATGTGT
  781 GCAAAGAAAA TCGTCGTCTT ACCAGGAGAC CATATTGGCC CTGAAATTGT TGCTTCTGCC
  841 TTGGAGGTTT TGAAAGTCGT TGAGAAGAAG CGACCTGAGT TAAAACTCGA GTTTGAAGAA
  901 CACAAGATTG GAGGTGCCTC TATTGATGCC TATGGAACCC CTTTGACTGA CGAGACTGTG
  961 AAGGCTTGTT TGGAAGCTGA CGGTGTTCTT TTGGTGCCGT TGGTGGTCCT GATGGACCAC
 1021 CCCAATTGTC GTCCTGAGCA GTTTATGAGC TCGTAGGAGT ATGGGTGTTT GGCACTCGAC
 1081 TGCCACTTGG CAGCAGTCTT AGTCAGTACA GCCATGAAGC TGGAATCGTT GAGGTTCGAT
1141 ATGGTTGTAC CGGAACTACT GAGCGTGATA CTTGGGGGAG CGTCACAT
//
Primer: ars_R1 RDB08940_A5Fge.seq
>06151_08940_A5Fg_ars_R1_H01_22.ab1
    1 AGGTTCGTTA TACTAATTAC TTAAGTACCT TTAACTAAAC AAAATGCCTA TATATATATT
   61 AATTTACAAT GAGTGTCAGA TAAGTCACTA TGTCCGAGTG GTTAAGGAGT TAGACTCGAA
  121 TTCGCAAGCT TAGCTACAAA TCCCACTGGC TATATGTATG CATTTGTGTT AAAAAAGTTT
  181 GTATAGATTA TTTAATCTAC TCAGCATTCT TTCTCTAAAT AGGAATTTGT TACTTAATGG
  241 AGAAAAAAAT GTTTCGATTT ACCTAGTGTA TTTGTTTGTA TACTCACGTT TAATTTCAAA
  301 CATCCATTCT ATCTTGTGTA ATTTTTGGCA TGGTGAAAAA GATAATCAGC CTTATAATCT
  361 TTACAAAAGT AAGAAATTCT GTAAATAAGC CTTAATGCCC TTGCTTTAAA TTAAAATGGT
  421 TCTTTTTCAT GATAATGTTT GCACTTTGTG AATATATTTT AGATAGTTCT GTGAGGTATA
  481 ATTAAGATGT TTTAGAGACT TATACAATTT TGTCTTTATA AATTCTTAAT TGATTTTACC
  541 ATCCCAGTTT AACTATGCTT CGTCGGCATC TCTGCACATG TCGTGTTTTC TTACCGTATT
  601 GTCCTACCAA GAACCTCTTT TTTGCTTGGA TCGAAATTAA AGGTTTAAAA GCAAAGTTAT
  661 GGATGCTAGA GTATTTCAAA GCTATTCAGC TAGAGCTGAG GGGATGAAAA ATCCCATTGC
  721 CAAGGAATTG TTGGCTTTGA TGGAAGAAAA GCAAAGCAAC TTGTCAGTCG CGGTCGATTT
  781 GACGAAGAAA TCCGAAATCT TAGAATTGGT AGATAAAATT GGACCCTATG TCTGTGTTAT
  841 CAAGACACAT ATTGACGTTG TCGAGGATTT CGACCAGGAT ATGGTAGAAA AACTGGTGGC
  901 CTTAGGTAAA AAGCATCGTT TTCTTATCTT TGAGGATCGC AAATTCGCAG ACATTGGAAA
  961 TACCGTCAAG CTACAATATG CATCTGGTGT GTACAAAATT GCTTCTTGGG CTCATATCAC
 1021 AAATTGCCAT ACAGTGCCAG GCGAGGGTAT ATACACGCTC AAAGAGTGGT TTACCTTTGG
 1081 ACGTGTCTCT GCTTTGCTGA AATGTCTCAA AGCTCTTTGC TACTGATCTA CAAGAGAATC
 1141 TAGATTGATG GAAGCCAACG ATTTTGCTTG GCTTATAGCT GCTCGTCGAA TTCCTACGTT
1201 CCAAGGCGAC CATCAC
//
Primer: f1ori_R3 RDB08940_A5Fgf.seq
>06151_08940_A5Fg_M13(-40)_A02_02.ab1
    1 AGGCCAGAGA TTCGAGCTCG GGAGCGCTAC CGTGAATGGG CTCAACCAAA CAATGAACCT
   61 TTTCTTCTGA TTTTCCTACC ACACCGGAAA GGGAGGCAGA AGGCAAAAGG CCCAAGCTAC
  121 CAGGAATGAC AGAAGCCTCA TCTGAAATAA TGTCACCAAA CAAGTTGTCA GTCAAAACAA
  181 CACCGTTAAG TGTACGAGGG CTCTTGACCA AAAGCATGGC TGCGGAGTCA ATGAGCTGGT
  241 TTTTTAAGGT AAGGTGAGGA TATTCCTCCT TAAAAATCTT AGCTACAGTC TTGCGCCAAA
  301 GACGAGAAGT TGCCAAAACA TTAGCTTTGT CGAGTAATGT GACGGGAGCA GGAGGGTTGG
  361 AAGTTTCAGC TAAGCGGCCG CGGGCCCAAC CTTCCAATTC ATTAAATCGA GTCCTCCATC
  421 AGATGATTTA CAAGGTTTTG CATAGAATCC CTAAGTAAGA GAAGTTGTTA GTGATTTCTT
  481 CAAGTTTACT AAAATTTACA TACAAATGGA TGATCTCTTC TCCATTGTTT CCTTTCTTCT
  541 TGCAAACGCG TTTTACAAAG AGATGACATG CTATTTTCTG ATTATTTTTT TCTATAGTTT
  601 TCTATTTTGT ACGTCCTTGG GAGTCCGAAA TGTAAAATCG GGGTATGGTT AGTAGCGGCA
  661 GCGTTCATTA TGGTATATAA AAGATAAAAA GTAGGGGCAA GCGAGACAGA ATGGGATACA
  721 AGGGCATCGT CTATAATTAT AGCTAAAAAT TGTATTTTAA TTTGTATTTT TTGTAATTTT
  781 ATTTCTCATT GTTTTACTTA AAATGAATGC GAATTAGAGA AAACTTCAAC GAAATGTCAA
  841 AATAAGCTCA GCAAATATAC AATTTTAGGG AAAGCGATCA GCAACTTATT CTCCGTTGTA
  901 GATTCTTTTT CTTCATAAAT TCCAACAATG TAACTGCCAA TTGATTTTTC TCTAGATGCT
  961 AATAAATTGA AACTAATGAA TATAATAACA CAGGTTTTGA TAAATTTGTA AACTCTTACT
 1021 CAAAAAAAGA ATGTTAAAAC TAATTTTAAC CCTTTTTATA ACGGCTCATG GCGTTAAAGA
 1081 GATAGTAAAA TCTACATTTT TTTATTTAAT GTCACACTGT CTGCGAATAT ATCCTAATAT
1141 TTTCGCTTAC TGCTGTTTTT AGTACCTATT TAACCTTTCA GA
//

Please visit Sequencing and PCR primers for primer information.


References

References

user report Eckler, A.M., Haploinsufficiency of the Sec7 guanine nucleotide exchange factor gea1 impairs septation in fission yeast. PLoS One, 8 (2): e56807 (2013). PMID 23457617.
user report Stigliano I.D., Glucosidase II beta subunit modulates N-glycan trimming in fission yeasts and mammals. Mol. Biol. Cell, 20, 3974-3984 (2009). PMID 19605557.

2019.03.08

GNP_filter3_RDBDEP_html_190307.pl