Sequencing and PCR primers for primer information.
S. pombe 3 ter end GFP(S65C)-FLAG-His6-tagged vector with nmt1 promoter
| Catalog number | RDB06145 |
|---|---|
| Resource name | pDUAL-GFH1c |
| Clone info. | S. pombe 3 ter end GFP(S65C)-FLAG-His6-tagged vector with nmt1 promoter. Multicopy marker, ura4+; Integration marker, leu1+. Gateway compatible. |
| Vector backbone | pDUAL (Plasmid) |
| Selectable markers | E. coli: Amp^r, Cm^r, ccdB. |
| Growth remarks | Use DB3.1, ccdB Survival or equivalent to amplify this clone. 30 degC is fine. |
| Reference of nucleotide sequence | AB364080.1 (DDBJ accession number) |
| Depositor | Yoshida, Minoru | Matsuyama, Akihisa | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Terms and conditions set forth by the DEPOSITOR | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation. |
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| Ordering | Please visit Information of Request for Distribution. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word] Schedule A (GFP-A) [Link] |
| Remarks | Use Survival2 (Invitrogen) or equivalent to amplify this clone. Please use Schedule A (GFP-A) additionally. |
[open/close]| Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
|---|---|---|---|
| RDB06145 | pDUAL-GFH1c | DNA solution |
Electronic file
| Electronic file | Sequence RDB06145z.seq from Depositor |
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Original reference
| original | Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162. |
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Further references such as user reports and related articles (go to bottom)
Featured content
| Featured content | Expression Vector Backbone of Schizosaccharomyces pombe (English text) |
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RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.
Data are summerized on test sheet below.
| Test sheet | RDB12163_A3H8.pdf |
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Nucleotide sequence of a portion of this resource (if available).
| Primer: nmt1Fw | Sequence: RDB12163_A3H8a.seq |
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>06145_12163_A3H8_nmt1Fw_A03_03.ab1 1 AAAAAATTAT TAAAATCAGG AAAAACGTAA CTCTCGGCTA CTGGATGGTT CAGTCACCCA 61 ACGATTACTG GGGAGAGAAA ACAGGGCAAA AGCAAAGCTT AAAGGAATCC GATTGTCATT 121 CGGCAATGTG CAGCGAAACT AAAAACCGGA TAATGGACCT GTTAATCGAA ACATTGAAGA 181 TATATAAAGG AAGAGGAATC CTGGCATATC ATCAATTGAA TAAGTTGAAT TAATTATTTC 241 AATCTCATTC TCACTTTCTG ACTTATAGTC GCTTTGTTAA AGCTAGCGTC GACAGGCCTG 301 GATCCGGATA TCAACAAGTT TGTACAAAAA AGCTGAACGA GAAACGTAAA ATGATATAAA 361 TATCAATATA TTAAATTAGA TTTTGCATAA AAAACAGACT ACATAATACT GTAAAACACA 421 ACATATCCAG TCACTATGGC GGCCGCATTA GGCACCCCAG GCTTTACACT TTATGCTTCC 481 GGCTCGTATA ATGTGTGGAT TTTGAGTTAG GATCCGTCGA GATTTTCAGG AGCTAAGGAA 541 GCTAAAATGG AGAAAAAAAT CACTGGATAT ACCACCGTTG ATATATCCCA ATGGCATCGT 601 AAAGAACATT TTGAGGCATT TCAGTCAGTT GCTCAATGTA CCTATAACCA GACCGTTCAG 661 CTGGATATTA CGGCCTTTTT AAAGACCGTA AAGAAAAATA AGCACAAGTT TTATCCGGCC 721 TTTATTCACA TTCTTGCCCG CCTGATGAAT GCTCATCCGG AATTCCGTAT GGCAATGAAA 781 GACGGTGAGC TGGTGATATG GGATAGTGTT CACCCTTGTT ACACCGTTTT CCATGAGCAA 841 ACTGAAACGT TTTCATCGCT CTGGAGTGAA TACCACGACG ATTTCCGGCA GTTTCTACAC 901 ATATATTCGC AAGATGTGGC GTGTTACGGT GAAAACCTGG CCTATTTCCC TAAAGGGTTT 961 ATTGAGAATA TGTTTTTTCG TCTCAGCCAA TCCCTGGGTG AGTTTCACCA GTTTTTGATT 1021 TAAACGTGGC CAATATGGAC CACTTTCTTC GCCCCCGTTT TCACCATGGC AAATATATAC 1081 GCAAGCGACA GGTGCTGATG CGCTTGGCCG AATCAGATCC ATTCAATGCC GTTGGTGAAA 1141 TGGCCTTCCA TGTGTTCCGG GGCC // |
| Primer: ADH_rev | Sequence: RDB12163_A3H8b.seq |
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>06145_12163_A3H8_ADHrev_B03_06.ab1 1 TTAGGCTCTC AAAGATAAGA ATTTTCGTTT TAAACCTAAG AGTCACTTTA AAATTTGTAT 61 ACACTTATTT TTTTTATAAC TTATTTAATA ATAAAAATCA TAAATCATAA GAAATTCGCT 121 TATTTAGAAG TGGCGCGCCG GATCCTCTAG AGTCGAGATC TCAGTGGTGA TGGTGGTGAT 181 GTTTGTCATC GTCGTCCTTG TAGTCCATGG GTTTGTATAG TTCATCCATG CCATGTGTAA 241 TCCCAGCAGC TGTTACAAAC TCAAGAAGGA CCATGTGGTC TCTCTTTTCG TTGGGATCTT 301 TCGAAAGGGC AGATTGTGTG GACAGGTAAT GGTTGTCTGG TAAAAGGACA GGGCCATCGC 361 CAATTGGAGT ATTTTGTTGA TAATGGTCTG CTAGTTGAAC GCTTCCATCT TCAATGTTGT 421 GTCTAATTTT GAAGTTAACT TTGATTCCAT TCTTTTGTTT GTCTGCCATG ATGTATACAT 481 TGTGTGAGTT ATAGTTGTAT TCCAATTTGT GTCCAAGAAT GTTTCCATCT TCTTTAAAAT 541 CAATACCTTT TAACTCGATT CTATTAACAA GGGTATCACC TTCAAACTTG ACTTCAGCAC 601 GTGTCTTGTA GTTCCCGTCA TCTTTGAAAA ATATAGTTCT TTCCTGTACA TAGCCTTCGG 661 GCATGGCACT CTTGAAAAAG TCATGCTGTT TCATGTGATC TGGGTATCTT GAAAAGCATT 721 GAACACCATA ACAGAAAGTA GTGACAAGTG TTGGCCACGG AACAGGTAGT TTTCCAGTAG 781 TGCAAATAAA TTTAAGGGTA AGTTTTCCGT ATGTTGCATC ACCTTCACCC TCTCCACTGA 841 CAGAAAATTT GTGCCCATTA ACATCACCAT CTAATTCAAC AAGAATTGGG ACAACTCCAG 901 TGAAAAGTTC TTCTCCTTTA CTCATGATAT CAACCACTTT GTACAAGAAA GCCTGGAACG 961 AGAAACGTAA AATGATATAA AATATCATAT ATTAAATTAG ATTTTTGCAT AAAAAACAGA 1021 CTACATATAC TTGTTAAAAC ACACATATCC AGTCACTAAT GGTTCGACTT GCCAGACCTG 1081 GCCTGTGTAT GAGCAGTCTG GACCAGCTTA TATTCCCCAG AAACATTCAG G // |
Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
2019.09.27
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