RDB06145 - pDUAL-GFH1c

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pDUAL-GFH1c (#RDB06145)

S. pombe 3 ter end GFP(S65C)-FLAG-His6-tagged vector with nmt1 promoter

Clone info.	S. pombe 3 ter end GFP(S65C)-FLAG-His6-tagged vector with nmt1 promoter. Multicopy marker, ura4+; Integration marker, leu1+.
Comment	Gateway compatible.
Vector backbone	pDUAL (RDB06178) (Plasmid)
Selectable markers	E. coli: Amp^r, Cm^r, ccdB.
Growth remarks	Use DB3.1, ccdB Survival or equivalent to amplify this clone. 30 degC is fine.
Reference of insert sequence	AB364080.1 (DDBJ accession number)
Depositor\|Developer	Yoshida, Minoru \| Matsuyama, Akihisa \|

Sequence	RDB06145z.seq

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.

Remarks Use Survival2 (Invitrogen) or equivalent to amplify this clone.

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Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.
Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
Remarks	Use Survival2 (Invitrogen) or equivalent to amplify this clone.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件	利用者は、研究成果の公表にあたって寄託者の指定する文献 (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) の引用と、　寄託者への謝辞の表明を必要とする。　利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。　寄託者から要請がある場合、理研ＢＲＣは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。
備考	このクローンの増殖にはSurvival2 (Invitrogen) あるいは同等の宿主を使用してください。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB06145	pDUAL-GFH1c	DNA solution

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pDUAL-GFH1c was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB06145).

Reference section:

Matsuyama, A., Shirai, A., Yashiroda, Y., Kamata, A., Horinouchi, S., Yoshida, M., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB12163_A3H8.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: nmt1Fw Sequence file: RDB12163_A3H8a.seq
>06145_12163_A3H8_nmt1Fw_A03_03.ab1 1 AAAAAATTAT TAAAATCAGG AAAAACGTAA CTCTCGGCTA CTGGATGGTT CAGTCACCCA 61 ACGATTACTG GGGAGAGAAA ACAGGGCAAA AGCAAAGCTT AAAGGAATCC GATTGTCATT 121 CGGCAATGTG CAGCGAAACT AAAAACCGGA TAATGGACCT GTTAATCGAA ACATTGAAGA 181 TATATAAAGG AAGAGGAATC CTGGCATATC ATCAATTGAA TAAGTTGAAT TAATTATTTC 241 AATCTCATTC TCACTTTCTG ACTTATAGTC GCTTTGTTAA AGCTAGCGTC GACAGGCCTG 301 GATCCGGATA TCAACAAGTT TGTACAAAAA AGCTGAACGA GAAACGTAAA ATGATATAAA 361 TATCAATATA TTAAATTAGA TTTTGCATAA AAAACAGACT ACATAATACT GTAAAACACA 421 ACATATCCAG TCACTATGGC GGCCGCATTA GGCACCCCAG GCTTTACACT TTATGCTTCC 481 GGCTCGTATA ATGTGTGGAT TTTGAGTTAG GATCCGTCGA GATTTTCAGG AGCTAAGGAA 541 GCTAAAATGG AGAAAAAAAT CACTGGATAT ACCACCGTTG ATATATCCCA ATGGCATCGT 601 AAAGAACATT TTGAGGCATT TCAGTCAGTT GCTCAATGTA CCTATAACCA GACCGTTCAG 661 CTGGATATTA CGGCCTTTTT AAAGACCGTA AAGAAAAATA AGCACAAGTT TTATCCGGCC 721 TTTATTCACA TTCTTGCCCG CCTGATGAAT GCTCATCCGG AATTCCGTAT GGCAATGAAA 781 GACGGTGAGC TGGTGATATG GGATAGTGTT CACCCTTGTT ACACCGTTTT CCATGAGCAA 841 ACTGAAACGT TTTCATCGCT CTGGAGTGAA TACCACGACG ATTTCCGGCA GTTTCTACAC 901 ATATATTCGC AAGATGTGGC GTGTTACGGT GAAAACCTGG CCTATTTCCC TAAAGGGTTT 961 ATTGAGAATA TGTTTTTTCG TCTCAGCCAA TCCCTGGGTG AGTTTCACCA GTTTTTGATT 1021 TAAACGTGGC CAATATGGAC CACTTTCTTC GCCCCCGTTT TCACCATGGC AAATATATAC 1081 GCAAGCGACA GGTGCTGATG CGCTTGGCCG AATCAGATCC ATTCAATGCC GTTGGTGAAA 1141 TGGCCTTCCA TGTGTTCCGG GGCC //
Primer: ADH_rev Sequence file: RDB12163_A3H8b.seq
>06145_12163_A3H8_ADHrev_B03_06.ab1 1 TTAGGCTCTC AAAGATAAGA ATTTTCGTTT TAAACCTAAG AGTCACTTTA AAATTTGTAT 61 ACACTTATTT TTTTTATAAC TTATTTAATA ATAAAAATCA TAAATCATAA GAAATTCGCT 121 TATTTAGAAG TGGCGCGCCG GATCCTCTAG AGTCGAGATC TCAGTGGTGA TGGTGGTGAT 181 GTTTGTCATC GTCGTCCTTG TAGTCCATGG GTTTGTATAG TTCATCCATG CCATGTGTAA 241 TCCCAGCAGC TGTTACAAAC TCAAGAAGGA CCATGTGGTC TCTCTTTTCG TTGGGATCTT 301 TCGAAAGGGC AGATTGTGTG GACAGGTAAT GGTTGTCTGG TAAAAGGACA GGGCCATCGC 361 CAATTGGAGT ATTTTGTTGA TAATGGTCTG CTAGTTGAAC GCTTCCATCT TCAATGTTGT 421 GTCTAATTTT GAAGTTAACT TTGATTCCAT TCTTTTGTTT GTCTGCCATG ATGTATACAT 481 TGTGTGAGTT ATAGTTGTAT TCCAATTTGT GTCCAAGAAT GTTTCCATCT TCTTTAAAAT 541 CAATACCTTT TAACTCGATT CTATTAACAA GGGTATCACC TTCAAACTTG ACTTCAGCAC 601 GTGTCTTGTA GTTCCCGTCA TCTTTGAAAA ATATAGTTCT TTCCTGTACA TAGCCTTCGG 661 GCATGGCACT CTTGAAAAAG TCATGCTGTT TCATGTGATC TGGGTATCTT GAAAAGCATT 721 GAACACCATA ACAGAAAGTA GTGACAAGTG TTGGCCACGG AACAGGTAGT TTTCCAGTAG 781 TGCAAATAAA TTTAAGGGTA AGTTTTCCGT ATGTTGCATC ACCTTCACCC TCTCCACTGA 841 CAGAAAATTT GTGCCCATTA ACATCACCAT CTAATTCAAC AAGAATTGGG ACAACTCCAG 901 TGAAAAGTTC TTCTCCTTTA CTCATGATAT CAACCACTTT GTACAAGAAA GCCTGGAACG 961 AGAAACGTAA AATGATATAA AATATCATAT ATTAAATTAG ATTTTTGCAT AAAAAACAGA 1021 CTACATATAC TTGTTAAAAC ACACATATCC AGTCACTAAT GGTTCGACTT GCCAGACCTG 1081 GCCTGTGTAT GAGCAGTCTG GACCAGCTTA TATTCCCCAG AAACATTCAG G //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162. [link to RRC of NBRP]
reference	Snaith, H.A., New and old reagents for fluorescent protein tagging of microtubules in fission yeast; experimental and critical evaluation. Methods Cell Biol., 97: 147-172 (2010). PMID 20719270. [link to RRC of NBRP]
user_report	Poulsen, E.G., UBL/BAG-domain co-chaperones cause cellular stress upon overexpression through constitutive activation of Hsf1. Cell Stress Chaperones 22 (1): 143-154 (2017). PMID 27966061. [link to RRC of NBRP]
user_report	Spivey, E.C., An aging-independent replicative lifespan in a symmetrically dividing eukaryote. Elife 6. pii: e20340 (2017). PMID 28139976. [link to RRC of NBRP]

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