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pDUAL-FFH1c

S. pombe 3 ter end FLAG2-His6-tagged vector with nmt1 promoter

Catalog number RDB06142
Resource name pDUAL-FFH1c
Clone info. S. pombe 3 ter end FLAG2-His6-tagged vector with nmt1 promoter. Multicopy marker, ura4+; Integration marker, leu1+.
Gateway compatible.
Vector backbone pDUAL (Plasmid)
Selectable markers E. coli: Amp^r, Cm^r, ccdB.
Growth remarks Use DB3.1, ccdB Survival or equivalent to amplify this clone. 30 degC is fine.
Reference of nucleotide sequence AB364077.1 (DDBJ accession number)
Depositor|Developer Yoshida, Minoru | Matsuyama, Akihisa |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
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Remarks Use Survival2 (Invitrogen) or equivalent to amplify this clone.
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RDB06142 pDUAL-FFH1c DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Electronic file Sequence RDB06142z.seq

Original reference

original Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Expression Vector Backbone of Schizosaccharomyces pombe (English text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB18236_B0G9p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: nmt1Fw
Region: nmt1 pro,attB1,CmR
Sequence file: RDB18236_B0G9a.seq check
>06142_18236_B0G9_2_nmt1Fw_E02_14_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNGTAAC TCTCNGCTAN TGGNNGGTTC AGTCACCCAA
   61 CGATTACTGG GGANANAAAA CAGGGCAAAA GCAAAGCTTA AAGGAATCCG ATTGTCATTC
  121 GGCAATGTGC AGCGAAACTA AAAACCGGAT AATGGACCTG TTAATCGAAA CATTGAAGAT
  181 ATATAAAGGA AGAGGAATCC TGGCATATCA TCAATTGAAT AAGTTGAATT AATTATTTCA
  241 ATCTCATTCT CACTTTCTGA CTTATAGTCG CTTTGTTAAA GCTAGCGTCG ACAGGCCTGG
  301 ATCCGGATAT CAACAAGTTT GTACAAAAAA GCTGAACGAG AAACGTAAAA TGATATAAAT
  361 ATCAATATAT TAAATTAGAT TTTGCATAAA AAACAGACTA CATAATACTG TAAAACACAA
  421 CATATCCAGT CACTATGGCG GCCGCATTAG GCACCCCAGG CTTTACACTT TATGCTTCCG
  481 GCTCGTATAA TGTGTGGATT TTGAGTTAGG ATCCGTCGAG ATTTTCAGGA GCTAAGGAAG
  541 CTAAAATGGA GAAAAAAATC ACTGGATATA CCACCGTTGA TATATCCCAA TGGCATCGTA
  601 AAGAACATTT TGAGGCATTT CAGTCAGTTG CTCAATGTAC CTATAACCAG ACCGTTCANC
  661 TGGATATTAC GGCCTTTTTA AAGACCGTAA AGAAAAATAA GCACAAGTTT TATCCGGCCT
  721 TTATTCACAT TCTTGCCCGC CNGATGAATG CTCATCCGGA ATTCCGTATG GCAATGAAAG
  781 ANGGTGANCT GGTGATATGG GNATAGTGTT CACCCTNNNT ACACCGTTTT NNNTGANCAA
  841 ACNNAAACGT TTTTCNTCGC TCTNGANTGA ATANCACNNC NANTNNCGGC ANTTTCTANN
901 CATATATTCN NAAGANGTNN NNNNNNNN
//
Primer: ADHrev
Region: ADH1 ter,6XHis,FLAGX2,attB2,ccdB
Sequence file: RDB18236_B0G9b.seq check
>06142_18236_B0G9_2_ADHrev_F02_17_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNAGA NTTTCGTTNN NNNNCTAAGA NTCACTTTAA AATTTGTATA
   61 CACTTATTTT TTTTATAACT TATTTAATAA TAAAAATCAT AAATCATAAG AAATTCGCTT
  121 ATTTAGAAGT GGCGCGCCGG ATCCTCTAGA GTCGAGATCT CAGTGGTGAT GGTGGTGATG
  181 TTTGTCATCG TCGTCCTTGT AGTCCATGGG TTTATCGTCA TCATCCTTAT AATCCATGAT
  241 ATCAACCACT TTGTACAAGA AAGCTGAACG AGAAACGTAA AATGATATAA ATATCAATAT
  301 ATTAAATTAG ATTTTGCATA AAAAACAGAC TACATAATAC TGTAAAACAC AACATATCCA
  361 GTCACTATGG TCGACCTGCA GACTGGCTGT GTATAAGGGA GCCTGACATT TATATTCCCC
  421 AGAACATCAG GTTAATGGCG TTTTTGATGT CATTTTCGCG GTGGCTGAGA TCAGCCACTT
  481 CTTCCCCGAT AACGGAGACC GGCACACTGG CCATATCGGT GGTCATCATG CGCCAGCTTT
  541 CATCCCCGAT ATGCACCACC GGGTAAAGTT CACGGGAGAC TTTATCTGAC AGCAGACGTG
  601 CACTGGCCAG GGGGATCACC ATCCGTCGCC CGGGCGTGTC AATAATATCA CTCTGTACAT
  661 CCACAAACAG ACGATAACGG CTCTCTCTTT TATAGGTGTA AACCTTAAAC TGCATTTCAC
  721 CAGCCCCNGT TCTCGTCAGC AAAAGAGCCG TTCATTTCAA TAAACCGGGC GANCTCAGCC
  781 ATCCCTTCCT GANTTTCCGC NTTNNAGCGT TCGGNACGCA NACGANGGNN TTCATTCTGC
841 NNGGTTGNGC TTANCAGANC GGNNNNATTG ACATCATATA TGCNNNNNNN NNN
//
Primer: ADH-Forward
Region: ADH1 ter,leu 1-5'
Sequence file: RDB18236_B0G9c.seq check
>06142_18236_B0G9_2_ADH-Forward_G02_20_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNCCN CNNCCGGNAN NNCCGCTAGG GNNAACAGGG TAATATACTA
   61 GTAAGATGAT TCTTTTATCG TCGATAACGA TGAGGATGTA GACGATTATG ATGAGAGTGA
  121 TTAGCTTCTA AAATTTGGTG TTTTCAAACG CTATTTACAG TACAATCTGA AAAAATTGGA
  181 TGAACATTGT AAATGGTAGG ATACGAGTTC CAATTAATGT ATTTTTTAAG TATTACACTT
  241 AGCTTATTAA TTTTATATAT ACATTCTACC TCTGTGGGTT TAAAGACAAC TCGTACACAT
  301 ACTCAAATTA TTTATGAACA TTATAATGCA TATTCGTACT GTCTATTAAA CATTTTTTTG
  361 ACATTAAAAA TTTCGTTTAC TAACGTAGAA AGCGCTGATA ATTGAGGCTA CTATTTTAAT
  421 ATCTAGAAAC AAAGATTCAT TAATTAATTA AGTAAGCTCC AGGATACTTG TATATTTCGT
  481 TAAAAAAGCG CAATTTCAAC AATTCCTATG AACATCCATG ATTTCATCAC AAAATCAAAC
  541 ATATCAGGAT TTAACGTGAA TTATTTGCTT TCTTGTGATT TTCATCTGAT TTCTGGTCAT
  601 TTACGTTACT GTAAACAAGC TTTTTAAAAA AAGGATTTTT TACGCAATAT TATCGATATC
  661 CCAATCTGTA GTATAAAAAG TGCAACAGTG TTGAGTTTCC CGAAACCAGG CATTTTTGCG
  721 GTAGGGTCTG AAACTTTCAC GAAACCATTT CACAACAAAA CTTCTTCNAC AACATGTGTG
  781 CAAAGAAAAT CGTCGTCTTA NCAGGANANC ATATTGGCCC NGAAATTGTT GCTTCTGNCN
  841 NNGNNGTTTT GAAANNCGTN NANAANAANC GNNNNNNNTT AAAACNCGNN NTTGNANGNN
  901 NNCNANNNNN NNNGNNGNNN NNNNTNNNNN NCNNNNNGNN ANNNCNNNTT NNNNNNNNNA
961 NNNNNNNNAN NNNNNNNNN
//
Primer: ars_R1
Region: ars 1,ura 4+
Sequence file: RDB18236_B0G9d.seq check
>06142_18236_B0G9_2_ars_R1_H02_23_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNAN TTAAGTACCT TTNNCTAAAC NAAATGCCTA TATATATATT
   61 AATTTACAAT GAGTGTCAGA TAAGTCACTA TGTCCGAGTG GTTAAGGAGT TAGACTCGAA
  121 TTCGCAAGCT TAGCTACAAA TCCCACTGGC TATATGTATG CATTTGTGTT AAAAAAGTTT
  181 GTATAGATTA TTTAATCTAC TCAGCATTCT TTCTCTAAAT AGGAATTTGT TACTTAATGG
  241 AGAAAAAAAT GTTTCGATTT ACCTAGTGTA TTTGTTTGTA TACTCACGTT TAATTTCAAA
  301 CATCCATTCT ATCTTGTGTA ATTTTTGGCA TGGTGAAAAA GATAATCAGC CTTATAATCT
  361 TTACAAAAGT AAGAAATTCT GTAAATAAGC CTTAATGCCC TTGCTTTAAA TTAAAATGGT
  421 TCTTTTTCAT GATAATGTTT GCACTTTGTG AATATATTTT AGATAGTTCT GTGAGGTATA
  481 ATTAAGATGT TTTAGAGACT TATACAATTT TGTCTTTATA AATTCTTAAT TGATTTTACC
  541 ATCCCAGTTT AACTATGCTT CGTCGGCATC TCTGCACATG TCGTGTTTTC TTACCGTATT
  601 GTCCTACCAA GAACCTCTTT TTTGCTTGGA TCGAAATTAA AGGTTTAAAA GCAAAGTTAT
  661 GGATGCTAGA GTATTTCNAA GCTATTCAGC TAGAGCTGAN GGGANGAAAA ATCCCATTGC
  721 CNAGGAATTG TTGGCTTTGA TGGAAGAAAA GCAAAGCAAC TTGTCAGTCG CGGNCGANTT
  781 GACNAAGAAA TCCNAAATCT TANNATTGGN AGATAAAATT GNANCCTANG TCTGTGNTAT
  841 CNNGANNCAT ANNGNNNTTN NCNANGNNTT NNANCNNNNN NNGGNANAAA AANTNNNNNN
901 NNTTNNGNNA AAANNATCNN TTNNNNNNNN TNNANNNNNN NNNN
//
Primer: f1ori_R3
Region: leu 1-3',ars 1
Sequence file: RDB18236_B0G9e.seq check
>06142_18236_B0G9_2_f1ori_R3_A03_03_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNCGCACANN NGCGTAANGA NAAANTACCG
   61 CATCAGGCGC CATTCGCNNT TCAGGCTGCG CAACTGTTGG GAAGGGCGAT CGGTGCGGGC
  121 CTCTTCGCTA TTACGCCAGC TGGCGAAAGG GGGATGTGCT GCAAGGCGAT TAAGTTGGGT
  181 AACGCCAGGG TTTTCCCAGT CACGACGTTG TAAAACGACG GCCAGTGAAT TCGAGCTCGG
  241 GAGCGCTACC GTGAATGGGC TCAACCAAAC AATGAACCTT TTCTTCTGAT TTTCCTACCA
  301 CACCGGAAAG GGAGGCAGAA GGCAAAAGGC CCAAGCTACC AGGAATGACA GAAGCCTCAT
  361 CTGAAATAAT GTCACCAAAC AAGTTGTCAG TCAAAACAAC ACCGTTAAGT GTACGAGGGC
  421 TCTTGACCAA AAGCATGGCT GCGGAGTCAA TGAGCTGGTT TTTTAAGGTA AGGTGAGGAT
  481 ATTCCTCCTT AAAAATCTTA GCTACAGTCT TGCGCCAAAG ACGAGAAGTT GCCAAAACAT
  541 TAGCTTTGTC GAGTAATGTG ACGGGAGCAG GAGGGTTGGA AGTTTCAGCT AAGCGGCCGC
  601 GGGCCCAACC TTCCAATTCA TTAAATCGAG TCCTCCATCA GATGATTTAC AAGGTTTTGC
  661 ATAGAATCCC TAAGTAAGAG AAGTTGTTAG TGATTTCTTC AAGTTTACTA ANATTTACAT
  721 ACAAATGGAT GATCTCTTCT CCATTGTTTC CTTTCTTCTT GCAAACGCGT TTTACNAAGA
  781 GNTGACATGC TATTTTCTGA TTATTTTTTT CTATAGTTTT CTATTTNGNA CGTCCTNNGG
  841 NNTCCNAAAN GNAAAATCGG GGNATGGTNN GNANCGGCAN CNTTCATTAN NGNANATNAA
901 AANNTNAAAA ANNNNNGGNN NNNNNNNN
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162.
reference Yashiroda, Y., A novel yeast cell-based screen identifies flavone as a tankyrase inhibitor. Biochem. Biophys. Res. Commun., 394, 569-573 (2010). PMID 20214890.
reference Nishimura, S., Marine antifungal theonellamides target 3beta-hydroxysterol to activate Rho1 signaling. Nat. Chem. Biol., 6, 519-526 (2010). PMID 20543850.
user report Girdwood, D., Constitutively active Cullin-RING-Ligases fail to rescue loss of NEDD8 conjugation in Schizosaccharomyces pombe. FEBS Lett., 586 (10): 1522-1528 (2012). PMID 22673520.
user report Anders, A., Improved tools for efficient mapping of fission yeast genes: identification of microtubule nucleation modifier mod22-1 as an allele of chromatin- remodelling factor gene swr1. Yeast 25 (12): 913-925 (2008). PMID 19160458.

2020.10.02

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