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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pDUAL-FFH1c

S. pombe 3 ter end FLAG2-His6-tagged vector with nmt1 promoter

Catalog number RDB06142
Resource name pDUAL-FFH1c
Clone info. S. pombe 3 ter end FLAG2-His6-tagged vector with nmt1 promoter. Multicopy marker, ura4+; Integration marker, leu1+.
Comment Gateway compatible.
Vector backbone pDUAL (Plasmid)
Selectable markers E. coli: Amp^r, Cm^r, ccdB.
Growth remarks Use DB3.1, ccdB Survival or equivalent to amplify this clone. 30 degC is fine.
Reference of insert sequence AB364077.1 (DDBJ accession number)
Depositor|Developer Yoshida, Minoru | Matsuyama, Akihisa |
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External Database

            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
            Ordering Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
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            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            Remarks Use Survival2 (Invitrogen) or equivalent to amplify this clone.
            提供案内 (日本国内) [open/close]

            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) の引用と、 寄託者への謝辞の表明を必要とする。 利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。 寄託者から要請がある場合、理研BRCは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
            備考 このクローンの増殖にはSurvival2 (Invitrogen) あるいは同等の宿主を使用してください。

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            RDB06142 pDUAL-FFH1c DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Sequence RDB06142z.seq

            Original reference

            original Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Expression Vector Backbone of Schizosaccharomyces pombe (English text)

            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB18236_B0G9p1-1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: nmt1Fw
            Region: nmt1 pro,attB1,CmR
            Sequence file: RDB18236_B0G9a.seq check
            >06142_18236_B0G9_2_nmt1Fw_E02_14_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNNNNGTAAC TCTCNGCTAN TGGNNGGTTC AGTCACCCAA
               61 CGATTACTGG GGANANAAAA CAGGGCAAAA GCAAAGCTTA AAGGAATCCG ATTGTCATTC
              121 GGCAATGTGC AGCGAAACTA AAAACCGGAT AATGGACCTG TTAATCGAAA CATTGAAGAT
              181 ATATAAAGGA AGAGGAATCC TGGCATATCA TCAATTGAAT AAGTTGAATT AATTATTTCA
              241 ATCTCATTCT CACTTTCTGA CTTATAGTCG CTTTGTTAAA GCTAGCGTCG ACAGGCCTGG
              301 ATCCGGATAT CAACAAGTTT GTACAAAAAA GCTGAACGAG AAACGTAAAA TGATATAAAT
              361 ATCAATATAT TAAATTAGAT TTTGCATAAA AAACAGACTA CATAATACTG TAAAACACAA
              421 CATATCCAGT CACTATGGCG GCCGCATTAG GCACCCCAGG CTTTACACTT TATGCTTCCG
              481 GCTCGTATAA TGTGTGGATT TTGAGTTAGG ATCCGTCGAG ATTTTCAGGA GCTAAGGAAG
              541 CTAAAATGGA GAAAAAAATC ACTGGATATA CCACCGTTGA TATATCCCAA TGGCATCGTA
              601 AAGAACATTT TGAGGCATTT CAGTCAGTTG CTCAATGTAC CTATAACCAG ACCGTTCANC
              661 TGGATATTAC GGCCTTTTTA AAGACCGTAA AGAAAAATAA GCACAAGTTT TATCCGGCCT
              721 TTATTCACAT TCTTGCCCGC CNGATGAATG CTCATCCGGA ATTCCGTATG GCAATGAAAG
              781 ANGGTGANCT GGTGATATGG GNATAGTGTT CACCCTNNNT ACACCGTTTT NNNTGANCAA
              841 ACNNAAACGT TTTTCNTCGC TCTNGANTGA ATANCACNNC NANTNNCGGC ANTTTCTANN
            901 CATATATTCN NAAGANGTNN NNNNNNNN
            //
            Primer: ADHrev
            Region: ADH1 ter,6XHis,FLAGX2,attB2,ccdB
            Sequence file: RDB18236_B0G9b.seq check
            >06142_18236_B0G9_2_ADHrev_F02_17_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNAGA NTTTCGTTNN NNNNCTAAGA NTCACTTTAA AATTTGTATA
               61 CACTTATTTT TTTTATAACT TATTTAATAA TAAAAATCAT AAATCATAAG AAATTCGCTT
              121 ATTTAGAAGT GGCGCGCCGG ATCCTCTAGA GTCGAGATCT CAGTGGTGAT GGTGGTGATG
              181 TTTGTCATCG TCGTCCTTGT AGTCCATGGG TTTATCGTCA TCATCCTTAT AATCCATGAT
              241 ATCAACCACT TTGTACAAGA AAGCTGAACG AGAAACGTAA AATGATATAA ATATCAATAT
              301 ATTAAATTAG ATTTTGCATA AAAAACAGAC TACATAATAC TGTAAAACAC AACATATCCA
              361 GTCACTATGG TCGACCTGCA GACTGGCTGT GTATAAGGGA GCCTGACATT TATATTCCCC
              421 AGAACATCAG GTTAATGGCG TTTTTGATGT CATTTTCGCG GTGGCTGAGA TCAGCCACTT
              481 CTTCCCCGAT AACGGAGACC GGCACACTGG CCATATCGGT GGTCATCATG CGCCAGCTTT
              541 CATCCCCGAT ATGCACCACC GGGTAAAGTT CACGGGAGAC TTTATCTGAC AGCAGACGTG
              601 CACTGGCCAG GGGGATCACC ATCCGTCGCC CGGGCGTGTC AATAATATCA CTCTGTACAT
              661 CCACAAACAG ACGATAACGG CTCTCTCTTT TATAGGTGTA AACCTTAAAC TGCATTTCAC
              721 CAGCCCCNGT TCTCGTCAGC AAAAGAGCCG TTCATTTCAA TAAACCGGGC GANCTCAGCC
              781 ATCCCTTCCT GANTTTCCGC NTTNNAGCGT TCGGNACGCA NACGANGGNN TTCATTCTGC
            841 NNGGTTGNGC TTANCAGANC GGNNNNATTG ACATCATATA TGCNNNNNNN NNN
            //
            Primer: ADH-Forward
            Region: ADH1 ter,leu 1-5'
            Sequence file: RDB18236_B0G9c.seq check
            >06142_18236_B0G9_2_ADH-Forward_G02_20_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNCCN CNNCCGGNAN NNCCGCTAGG GNNAACAGGG TAATATACTA
               61 GTAAGATGAT TCTTTTATCG TCGATAACGA TGAGGATGTA GACGATTATG ATGAGAGTGA
              121 TTAGCTTCTA AAATTTGGTG TTTTCAAACG CTATTTACAG TACAATCTGA AAAAATTGGA
              181 TGAACATTGT AAATGGTAGG ATACGAGTTC CAATTAATGT ATTTTTTAAG TATTACACTT
              241 AGCTTATTAA TTTTATATAT ACATTCTACC TCTGTGGGTT TAAAGACAAC TCGTACACAT
              301 ACTCAAATTA TTTATGAACA TTATAATGCA TATTCGTACT GTCTATTAAA CATTTTTTTG
              361 ACATTAAAAA TTTCGTTTAC TAACGTAGAA AGCGCTGATA ATTGAGGCTA CTATTTTAAT
              421 ATCTAGAAAC AAAGATTCAT TAATTAATTA AGTAAGCTCC AGGATACTTG TATATTTCGT
              481 TAAAAAAGCG CAATTTCAAC AATTCCTATG AACATCCATG ATTTCATCAC AAAATCAAAC
              541 ATATCAGGAT TTAACGTGAA TTATTTGCTT TCTTGTGATT TTCATCTGAT TTCTGGTCAT
              601 TTACGTTACT GTAAACAAGC TTTTTAAAAA AAGGATTTTT TACGCAATAT TATCGATATC
              661 CCAATCTGTA GTATAAAAAG TGCAACAGTG TTGAGTTTCC CGAAACCAGG CATTTTTGCG
              721 GTAGGGTCTG AAACTTTCAC GAAACCATTT CACAACAAAA CTTCTTCNAC AACATGTGTG
              781 CAAAGAAAAT CGTCGTCTTA NCAGGANANC ATATTGGCCC NGAAATTGTT GCTTCTGNCN
              841 NNGNNGTTTT GAAANNCGTN NANAANAANC GNNNNNNNTT AAAACNCGNN NTTGNANGNN
              901 NNCNANNNNN NNNGNNGNNN NNNNTNNNNN NCNNNNNGNN ANNNCNNNTT NNNNNNNNNA
            961 NNNNNNNNAN NNNNNNNNN
            //
            Primer: ars_R1
            Region: ars 1,ura 4+
            Sequence file: RDB18236_B0G9d.seq check
            >06142_18236_B0G9_2_ars_R1_H02_23_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNAN TTAAGTACCT TTNNCTAAAC NAAATGCCTA TATATATATT
               61 AATTTACAAT GAGTGTCAGA TAAGTCACTA TGTCCGAGTG GTTAAGGAGT TAGACTCGAA
              121 TTCGCAAGCT TAGCTACAAA TCCCACTGGC TATATGTATG CATTTGTGTT AAAAAAGTTT
              181 GTATAGATTA TTTAATCTAC TCAGCATTCT TTCTCTAAAT AGGAATTTGT TACTTAATGG
              241 AGAAAAAAAT GTTTCGATTT ACCTAGTGTA TTTGTTTGTA TACTCACGTT TAATTTCAAA
              301 CATCCATTCT ATCTTGTGTA ATTTTTGGCA TGGTGAAAAA GATAATCAGC CTTATAATCT
              361 TTACAAAAGT AAGAAATTCT GTAAATAAGC CTTAATGCCC TTGCTTTAAA TTAAAATGGT
              421 TCTTTTTCAT GATAATGTTT GCACTTTGTG AATATATTTT AGATAGTTCT GTGAGGTATA
              481 ATTAAGATGT TTTAGAGACT TATACAATTT TGTCTTTATA AATTCTTAAT TGATTTTACC
              541 ATCCCAGTTT AACTATGCTT CGTCGGCATC TCTGCACATG TCGTGTTTTC TTACCGTATT
              601 GTCCTACCAA GAACCTCTTT TTTGCTTGGA TCGAAATTAA AGGTTTAAAA GCAAAGTTAT
              661 GGATGCTAGA GTATTTCNAA GCTATTCAGC TAGAGCTGAN GGGANGAAAA ATCCCATTGC
              721 CNAGGAATTG TTGGCTTTGA TGGAAGAAAA GCAAAGCAAC TTGTCAGTCG CGGNCGANTT
              781 GACNAAGAAA TCCNAAATCT TANNATTGGN AGATAAAATT GNANCCTANG TCTGTGNTAT
              841 CNNGANNCAT ANNGNNNTTN NCNANGNNTT NNANCNNNNN NNGGNANAAA AANTNNNNNN
            901 NNTTNNGNNA AAANNATCNN TTNNNNNNNN TNNANNNNNN NNNN
            //
            Primer: f1ori_R3
            Region: leu 1-3',ars 1
            Sequence file: RDB18236_B0G9e.seq check
            >06142_18236_B0G9_2_f1ori_R3_A03_03_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNCGCACANN NGCGTAANGA NAAANTACCG
               61 CATCAGGCGC CATTCGCNNT TCAGGCTGCG CAACTGTTGG GAAGGGCGAT CGGTGCGGGC
              121 CTCTTCGCTA TTACGCCAGC TGGCGAAAGG GGGATGTGCT GCAAGGCGAT TAAGTTGGGT
              181 AACGCCAGGG TTTTCCCAGT CACGACGTTG TAAAACGACG GCCAGTGAAT TCGAGCTCGG
              241 GAGCGCTACC GTGAATGGGC TCAACCAAAC AATGAACCTT TTCTTCTGAT TTTCCTACCA
              301 CACCGGAAAG GGAGGCAGAA GGCAAAAGGC CCAAGCTACC AGGAATGACA GAAGCCTCAT
              361 CTGAAATAAT GTCACCAAAC AAGTTGTCAG TCAAAACAAC ACCGTTAAGT GTACGAGGGC
              421 TCTTGACCAA AAGCATGGCT GCGGAGTCAA TGAGCTGGTT TTTTAAGGTA AGGTGAGGAT
              481 ATTCCTCCTT AAAAATCTTA GCTACAGTCT TGCGCCAAAG ACGAGAAGTT GCCAAAACAT
              541 TAGCTTTGTC GAGTAATGTG ACGGGAGCAG GAGGGTTGGA AGTTTCAGCT AAGCGGCCGC
              601 GGGCCCAACC TTCCAATTCA TTAAATCGAG TCCTCCATCA GATGATTTAC AAGGTTTTGC
              661 ATAGAATCCC TAAGTAAGAG AAGTTGTTAG TGATTTCTTC AAGTTTACTA ANATTTACAT
              721 ACAAATGGAT GATCTCTTCT CCATTGTTTC CTTTCTTCTT GCAAACGCGT TTTACNAAGA
              781 GNTGACATGC TATTTTCTGA TTATTTTTTT CTATAGTTTT CTATTTNGNA CGTCCTNNGG
              841 NNTCCNAAAN GNAAAATCGG GGNATGGTNN GNANCGGCAN CNTTCATTAN NGNANATNAA
            901 AANNTNAAAA ANNNNNGGNN NNNNNNNN
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162.
            reference Yashiroda, Y., A novel yeast cell-based screen identifies flavone as a tankyrase inhibitor. Biochem. Biophys. Res. Commun., 394, 569-573 (2010). PMID 20214890.
            reference Nishimura, S., Marine antifungal theonellamides target 3beta-hydroxysterol to activate Rho1 signaling. Nat. Chem. Biol., 6, 519-526 (2010). PMID 20543850.
            user_report Girdwood, D., Constitutively active Cullin-RING-Ligases fail to rescue loss of NEDD8 conjugation in Schizosaccharomyces pombe. FEBS Lett., 586 (10): 1522-1528 (2012). PMID 22673520.
            user_report Anders, A., Improved tools for efficient mapping of fission yeast genes: identification of microtubule nucleation modifier mod22-1 as an allele of chromatin- remodelling factor gene swr1. Yeast 25 (12): 913-925 (2008). PMID 19160458.

            2022.05.18

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