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pDUAL-FFH1c

S. pombe 3 ter end FLAG2-His6-tagged vector with nmt1 promoter

Catalog number RDB06142
Resource name pDUAL-FFH1c
Clone info. S. pombe 3 ter end FLAG2-His6-tagged vector with nmt1 promoter. Multicopy marker, ura4+; Integration marker, leu1+.
Gateway compatible.
Vector backbone pDUAL (Plasmid)
Selectable markers E. coli: Amp^r, Cm^r, ccdB.
Growth remarks Use DB3.1, ccdB Survival or equivalent to amplify this clone. 30 degC is fine.
Reference of nucleotide sequence AB364077.1 (DDBJ accession number)
Depositor Yoshida, Minoru | Matsuyama, Akihisa |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
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Material Transfer Agreement (MTA) [Word]
Remarks Use Survival2 (Invitrogen) or equivalent to amplify this clone.
Distribution information (domestic users) [open/close]


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RDB06142 pDUAL-FFH1c DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB06142z.seq from Depositor

Original reference

original Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Expression Vector Backbone of Schizosaccharomyces pombe (English text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB12158_A3H8.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: nmt1Fw | Sequence: RDB12158_A3H8a.seq
>06142_12158_A3H8_nmt1Fw_C02_08.ab1
    1 AAAAAATTTA TTAATCATGA AAACGTAACT CTCGGCTACT GGATGGTTCA GTCACCCAAC
   61 GATTACTGGG GAGAGAAAAC AGGGCAAAAG CAAAGCTTAA AGGAATCCGA TTGTCATTCG
  121 GCAATGTGCA GCGAAACTAA AAACCGGATA ATGGACCTGT TAATCGAAAC ATTGAAGATA
  181 TATAAAGGAA GAGGAATCCT GGCATATCAT CAATTGAATA AGTTGAATTA ATTATTTCAA
  241 TCTCATTCTC ACTTTCTGAC TTATAGTCGC TTTGTTAAAG CTAGCGTCGA CAGGCCTGGA
  301 TCCGGATATC AACAAGTTTG TACAAAAAAG CTGAACGAGA AACGTAAAAT GATATAAATA
  361 TCAATATATT AAATTAGATT TTGCATAAAA AACAGACTAC ATAATACTGT AAAACACAAC
  421 ATATCCAGTC ACTATGGCGG CCGCATTAGG CACCCCAGGC TTTACACTTT ATGCTTCCGG
  481 CTCGTATAAT GTGTGGATTT TGAGTTAGGA TCCGTCGAGA TTTTCAGGAG CTAAGGAAGC
  541 TAAAATGGAG AAAAAAATCA CTGGATATAC CACCGTTGAT ATATCCCAAT GGCATCGTAA
  601 AGAACATTTT GAGGCATTTC AGTCAGTTGC TCAATGTACC TATAACCAGA CCGTTCAGCT
  661 GGATATTACG GCCTTTTTAA AGACCGTAAA GAAAAATAAG CACAAGTTTT ATCCGGCCTT
  721 TATTCACATT CTTGCCCGCC TGATGAATGC TCATCCGGAA TTCCGTATGG CAATGAAAGA
  781 CGGTGAGCTG GTGATATGGG ATAGTGTTCA CCCTTGTTAC ACCGTTTTCC ATGAGCAAAC
  841 TGAAACGTTT TCATCGCTCT GGAGTGAATA CCACGACGAT TTCCGGCAGT TTCTACACAT
  901 ATATTCGCAA GATGTGGCGT GTTACGGTGA AAACCTGGCC TATTTCCCTA AAGGGTTTAT
  961 TGAGAATATG TTTTTCGTCT CAGCCAATCC CTGGTGAGTT TCACCAGTTT TGATTTAACG
 1021 TGGCCAATAT GGGACAACTT TCTTCGCCCC CGTTTTCACC ATGGGCAAAA TATATACGCA
 1081 GCCGACAGTG CTGGATGCCG CTGGCGATCA GGTCAATCAA TGGCGGATTG TGATGGATCT
1141 TCCATGTTTC GGACCAG
//
Primer: ADH_rev | Sequence: RDB12158_A3H8b.seq
>06142_12158_A3H8_ADHrev_D02_11.ab1
    1 GGGGGATCTC AGATAAGATT TTCGTTTTAA ACCTAAGAGT CACTTTAAAA TTTGTATACA
   61 CTTATTTTTT TTATAACTTA TTTAATAATA AAAATCATAA ATCATAAGAA ATTCGCTTAT
  121 TTAGAAGTGG CGCGCCGGAT CCTCTAGAGT CGAGATCTCA GTGGTGATGG TGGTGATGTT
  181 TGTCATCGTC GTCCTTGTAG TCCATGGGTT TATCGTCATC ATCCTTATAA TCCATGATAT
  241 CAACCACTTT GTACAAGAAA GCTGAACGAG AAACGTAAAA TGATATAAAT ATCAATATAT
  301 TAAATTAGAT TTTGCATAAA AAACAGACTA CATAATACTG TAAAACACAA CATATCCAGT
  361 CACTATGGTC GACCTGCAGA CTGGCTGTGT ATAAGGGAGC CTGACATTTA TATTCCCCAG
  421 AACATCAGGT TAATGGCGTT TTTGATGTCA TTTTCGCGGT GGCTGAGATC AGCCACTTCT
  481 TCCCCGATAA CGGAGACCGG CACACTGGCC ATATCGGTGG TCATCATGCG CCAGCTTTCA
  541 TCCCCGATAT GCACCACCGG GTAAAGTTCA CGGGAGACTT TATCTGACAG CAGACGTGCA
  601 CTGGCCAGGG GGATCACCAT CCGTCGCCCG GGCGTGTCAA TAATATCACT CTGTACATCC
  661 ACAAACAGAC GATAACGGCT CTCTCTTTTA TAGGTGTAAA CCTTAAACTG CATTTCACCA
  721 GCCCCTGTTC TCGTCAGCAA AAGAGCCGTT CATTTCAATA AACCGGGCGA CCTCAGCCAT
  781 CCCTTCCTGA TTTTCCGCTT TCCAGCGTTC GGCACGCAGA CGACGGGCTT CATTCTGCAT
  841 GGTTGTGCTT ACCAGACCGG AGATATTGAC ATCATATATG CCTTGAGCAA CTGATAGCTG
  901 TCGCTGTCAA CTGTCACTGT AATACGCTGC TTCATAGCAT ACCTCTTTTT TGACATACTT
  961 CGGGGTATAC ATATCAGTAT ATATTCTTTA TACCGCAAAA AATCAGCGCG CAAATACGCA
 1021 TACTGTTATC TGGGCTTTTA GTAGCCGGAT CCCAGGATCT TACGCCCCGC CCTGCCACTC
1081 ATCGCAGTAC TGATGTATCA TAAGCCAATT CTGCCGGACC ATGGCAG
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162.
reference Yashiroda, Y., A novel yeast cell-based screen identifies flavone as a tankyrase inhibitor. Biochem. Biophys. Res. Commun., 394, 569-573 (2010). PMID 20214890.
reference Nishimura, S., Marine antifungal theonellamides target 3beta-hydroxysterol to activate Rho1 signaling. Nat. Chem. Biol., 6, 519-526 (2010). PMID 20543850.
user report Girdwood, D., Constitutively active Cullin-RING-Ligases fail to rescue loss of NEDD8 conjugation in Schizosaccharomyces pombe. FEBS Lett., 586 (10): 1522-1528 (2012). PMID 22673520.
user report Anders, A., Improved tools for efficient mapping of fission yeast genes: identification of microtubule nucleation modifier mod22-1 as an allele of chromatin- remodelling factor gene swr1. Yeast 25 (12): 913-925 (2008). PMID 19160458.

2019.09.27

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