S. pombe 5 ter end His6-FLAG-GFP(S65C)-tagged vector with nmt1 (medium strength) promoter
Catalog number | RDB06134 |
---|---|
Resource name | pDUAL-HFG41c |
Clone info. | S. pombe 5 ter end His6-FLAG-GFP(S65C)-tagged vector with nmt1 (medium strength) promoter. Multicopy marker, ura4+; Integration marker, leu1+. Gateway compatible. |
Vector backbone | pDUAL (Plasmid) |
Selectable markers | E. coli: Amp^r, Cm^r, ccdB. |
Growth remarks | Use DB3.1, ccdB Survival or equivalent to amplify this clone. 30 degC is fine. |
Reference of nucleotide sequence | AB364075.1 (DDBJ accession number) |
Depositor | Yoshida, Minoru | Matsuyama, Akihisa | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation. |
---|---|
Ordering | Please visit Information of Request for Distribution. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word] Schedule A (GFP-A) [Link] |
Remarks | Use Survival2 (Invitrogen) or equivalent to amplify this clone. Please use Schedule A (GFP-A) additionally. |
Catalog # | Resource name | Availability | Shipping form | Fee (non-profit org.) |
---|---|---|---|---|
RDB06134 | pDUAL-HFG41c | Under QC test. Please contact us. | DNA solution |
Electronic file
Electronic file | Sequence RDB06134z.seq |
---|
Original reference
original | Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162. |
---|
Further references such as user reports and related articles (go to bottom)
Featured content
Featured content | Dnaconda's recommendation EXR0075j (Japanese text) |
---|---|
Featured content | Dnaconda's recommendation EXR0075e (English text) |
Featured content | Expression Vector Backbone of Schizosaccharomyces pombe (English text) |
Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.
Original, user report and related articles
2020.01.22
GNP_filter3_RDBDEP_html_191130.pl