RDB06134 - pDUAL-HFG41c

S. pombe 5 ter end His6-FLAG-GFP(S65C)-tagged vector with nmt1 (medium strength) promoter

Distribution information

Catalog number	RDB06134
Resource name	pDUAL-HFG41c
Clone info.	S. pombe 5 ter end His6-FLAG-GFP(S65C)-tagged vector with nmt1 (medium strength) promoter. Multicopy marker, ura4+; Integration marker, leu1+. Gateway compatible.
Vector backbone	pDUAL (Plasmid)
Selectable markers	Amp^r, Cm^r
Reference of nucleotide sequence	AB364075.1 (DDBJ accession number)
Depositor	Yoshida, Minoru \| Matsuyama, Akihisa \|

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
Ordering	Please visit Information of Request for Distribution. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word] Schedule A (GFP-A) [Link]
Remarks	((Additional form)) GFP-A Use Survival2 (Invitrogen) or equivalent to amplify this clone. Please use Schedule A (GFP-A) additionally.

Distribution information (domestic users)

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献 (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) の引用と、　寄託者への謝辞の表明を必要とする。　利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。　寄託者から要請がある場合、理研ＢＲＣは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。
提供依頼	手続きの詳細は、「提供申込みについて」をご覧ください。提供依頼書 [Word] 提供同意書 [Word] Schedule A（GFP-A, 英語版のみ） [Link]
備考	【追加提供依頼書】GFP-A このクローンの増殖にはSurvival2 (Invitrogen) あるいは同等の宿主を使用してください。 Schedule A（GFP-A, 英語版のみ）が追加で必要です。

References and tips

Sequence information

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB06134	pDUAL-HFG41c	DNA solution

Electronic file	Sequence RDB06134z.seq from Depositor

Featured content	Expression Vector Backbone of Schizosaccharomyces pombe (English text)

original	Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162.

This clone will be sequenced a portion and digested by restriction enzyme for examination.

original	Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162.
reference	Snaith, H.A., New and old reagents for fluorescent protein tagging of microtubules in fission yeast; experimental and critical evaluation. Methods Cell Biol., 97: 147-172 (2010). PMID 20719270.
user report	Srinivasan, R., Bacillus anthracis tubulin-related protein Ba-TubZ assembles force-generating polymers. Cytoskeleton (Hoboken) 68(9): 501-511 (2011). PMID 21780309.