RDB06133 - pDUAL-HFG1c

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pDUAL-HFG1c

S. pombe 5 ter end His6-FLAG-GFP(S65C)-tagged vector with nmt1 promoter

Catalog number	RDB06133
Resource name	pDUAL-HFG1c
Clone info.	S. pombe 5 ter end His6-FLAG-GFP(S65C)-tagged vector with nmt1 promoter. Multicopy marker, ura4+; Integration marker, leu1+. Gateway compatible.
Vector backbone	pDUAL (Plasmid)
Selectable markers	E. coli: Amp^r, Cm^r, ccdB.
Growth remarks	Use DB3.1, ccdB Survival or equivalent to amplify this clone. 30 degC is fine.
Reference of nucleotide sequence	AB364074.1 (DDBJ accession number)
Depositor	Yoshida, Minoru \| Matsuyama, Akihisa \|

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.

Ordering Please visit Information of Request for Distribution.
Order form [Credit Card Payment] [Bank Transfer or Check Payment]
Material Transfer Agreement (MTA) [Word]
Schedule A (GFP-A) [Link]

Remarks Use Survival2 (Invitrogen) or equivalent to amplify this clone.
Please use Schedule A (GFP-A) additionally.

Distribution information (domestic users) [open/close]

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
Ordering	Please visit Information of Request for Distribution. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word] Schedule A (GFP-A) [Link]
Remarks	Use Survival2 (Invitrogen) or equivalent to amplify this clone. Please use Schedule A (GFP-A) additionally.

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献 (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) の引用と、　寄託者への謝辞の表明を必要とする。　利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。　寄託者から要請がある場合、理研ＢＲＣは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。
提供依頼	手続きの詳細は、「提供申込みについて」をご覧ください。提供依頼書 [Word] 提供同意書 [Word] Schedule A（GFP-A, 英語版のみ） [Link]
備考	このクローンの増殖にはSurvival2 (Invitrogen) あるいは同等の宿主を使用してください。 Schedule A（GFP-A, 英語版のみ）が追加で必要です。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB06133	pDUAL-HFG1c	DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check

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References and tips

Electronic file

Electronic file	Sequence RDB06133z.seq

Original reference

original	Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162.

Further references such as user reports and related articles (go to bottom)

Featured content	Dnaconda's recommendation EXR0075j (Japanese text)
Featured content	Dnaconda's recommendation EXR0075e (English text)
Featured content	Expression Vector Backbone of Schizosaccharomyces pombe (English text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB12153_A3Gq.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: nmt1Fw Sequence file: RDB12153_A3Gqa.seq
>06133_12153_A3Gq_nmt1Fw_E03_15.ab1 1 ATGGATAATT TTTTAAATCA AGGGAAAACG TAACTCTCGG CTACTGGATG GTTCAGTCAC 61 CCAACGATTA CTGGGGAGAG AAAACAGGGC AAAAGCAAAG CTTAAAGGAA TCCGATTGTC 121 ATTCGGCAAT GTGCAGCGAA ACTAAAAACC GGATAATGGA CCTGTTAATC GAAACATTGA 181 AGATATATAA AGGAAGAGGA ATCCTGGCAT ATCATCAATT GAATAAGTTG AATTAATTAT 241 TTCAATCTCA TTCTCACTTT CTGACTTATA GTCGCTTTGT TAAAGCTAGC GTCGACAGGC 301 CTGGATCCTA TGCATCACCA CCATCATCAT ATGGATTATA AGGATGATGA CGATAAACCC 361 ATGGGTAAAG GAGAAGAACT TTTCACTGGA GTTGTCCCAA TTCTTGTTGA ATTAGATGGT 421 GATGTTAATG GGCACAAATT TTCTGTCAGT GGAGAGGGTG AAGGTGATGC AACATACGGA 481 AAACTTACCC TTAAATTTAT TTGCACTACT GGAAAACTAC CTGTTCCGTG GCCAACACTT 541 GTCACTACTT TCTGTTATGG TGTTCAATGC TTTTCAAGAT ACCCAGATCA CATGAAACAG 601 CATGACTTTT TCAAGAGTGC CATGCCCGAA GGCTATGTAC AGGAAAGAAC TATATTTTTC 661 AAAGATGACG GGAACTACAA GACACGTGCT GAAGTCAAGT TTGAAGGTGA TACCCTTGTT 721 AATAGAATCG AGTTAAAAGG TATTGATTTT AAAGAAGATG GAAACATTCT TGGACACAAA 781 TTGGAATACA ACTATAACTC ACACAATGTA TACATCATGG CAGACAAACA AAAGAATGGA 841 ATCAAAGTTA ACTTCAAAAT TAGACACAAC ATTGAAGATG GAAGCGTTCA ACTAGCAGAC 901 CATTATCAAC AAAATACTCC AATTGGCGAT GGCCCTGTCC TTTTACCAGA CAACCATTAC 961 CTGTCCACAC AATCTGCCCT TTCGAAAGAT CCCAACGAAA AGAGAGACCA CATGGTCCTT 1021 CTTGAGTTTG TAACAGCTGC TGGGATTACA CATGGCATGG ATGAACTATA CAAAGATATC 1081 ACAAGTTTGT ACAAAAAGCT GAACGAGAAA CGTTAAAATG ATATAATATC ATATTATTAA 1141 TTTAGATTTT GCATAAACCG GACTACTAAT AACTGTAACA CAACCATATC CAGTCACTTA 1201 TTGGGCGGGC GCCATTTAAG GAAACACTC //
Primer: ADH_rev Sequence file: RDB12153_A3Gqb.seq
>06133_12153_A3Gq_ADHrev_F03_18.ab1 1 GAAGGGAATT ACTAAAGAAT AAGAATTTTC GTTTTAAAAC CTAAGAGGTC ACTTTAAAAT 61 TTGTATACAC TTATTTTTTT TATAACTTAT TTAATAATAA AAATCATAAA TCATAAGAAA 121 TTCGCTTATT TAGAAGTGGC GCGCCGGATC CTCTAGAGTC GAGATCTCAT GATATCACCA 181 CTTTGTACAA GAAAGCTGAA CGAGAAACGT AAAATGATAT AAATATCAAT ATATTAAATT 241 AGATTTTGCA TAAAAAACAG ACTACATAAT ACTGTAAAAC ACAACATATC CAGTCACTAT 301 GGTCGACCTG CAGACTGGCT GTGTATAAGG GAGCCTGACA TTTATATTCC CCAGAACATC 361 AGGTTAATGG CGTTTTTGAT GTCATTTTCG CGGTGGCTGA GATCAGCCAC TTCTTCCCCG 421 ATAACGGAGA CCGGCACACT GGCCATATCG GTGGTCATCA TGCGCCAGCT TTCATCCCCG 481 ATATGCACCA CCGGGTAAAG TTCACGGGAG ACTTTATCTG ACAGCAGACG TGCACTGGCC 541 AGGGGGATCA CCATCCGTCG CCCGGGCGTG TCAATAATAT CACTCTGTAC ATCCACAAAC 601 AGACGATAAC GGCTCTCTCT TTTATAGGTG TAAACCTTAA ACTGCATTTC ACCAGCCCCT 661 GTTCTCGTCA GCAAAAGAGC CGTTCATTTC AATAAACCGG GCGACCTCAG CCATCCCTTC 721 CTGATTTTCC GCTTTCCAGC GTTCGGCACG CAGACGACGG GCTTCATTCT GCATGGTTGT 781 GCTTACCAGA CCGGAGATAT TGACATCATA TATGCCTTGA GCAACTGATA GCTGTCGCTG 841 TCAACTGTCA CTGTAATACG CTGCTTCATA GCATACCTCT TTTTGACATA CTTCGGGTAT 901 ACATATCAGT ATATATTCTT ATACCGCAAA AATCAGCGCG CAAATACGCA TACTGTTATC 961 TGGCTTTTAG TAAGCCGGAT CCACGCGTTT ACGCCCCGCC CTGCCACTCA TCGCAGTACT 1021 GTTGTAATTC ATTAAGCATT CTGCCGACAT GGCAGCCATC ACAAACGGCA TGATGACCCT 1081 GAATTCGCCA GCGGCCATCA GCACTGTCGC TTGCGTAATA AATATTGCCA TGTGAACCGG 1141 GGGGCGAAGA GTTGTTCATA TTTGGGCACA CGGTTTTAAA TCTCAGA //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162.
reference	Snaith, H.A., New and old reagents for fluorescent protein tagging of microtubules in fission yeast; experimental and critical evaluation. Methods Cell Biol., 97: 147-172 (2010). PMID 20719270.
user report	Srinivasan, R., Bacillus anthracis tubulin-related protein Ba-TubZ assembles force-generating polymers. Cytoskeleton (Hoboken) 68(9): 501-511 (2011). PMID 21780309.

2020.01.22

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