Sequencing and PCR primers for primer information.
S. pombe 5 ter end His6-FLAG-GFP(S65C)-tagged vector with nmt1 promoter
| Catalog number | RDB06133 |
|---|---|
| Resource name | pDUAL-HFG1c |
| Clone info. | S. pombe 5 ter end His6-FLAG-GFP(S65C)-tagged vector with nmt1 promoter. Multicopy marker, ura4+; Integration marker, leu1+. Gateway compatible. |
| Vector backbone | pDUAL (Plasmid) |
| Selectable markers | Amp^r, Cm^r |
| Reference of nucleotide sequence | AB364074.1 (DDBJ accession number) |
| Depositor | Yoshida, Minoru | Matsuyama, Akihisa | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Terms and conditions set forth by the DEPOSITOR | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation. |
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| Ordering | Please visit Information of Request for Distribution. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word] Schedule A (GFP-A) [Link] |
| Remarks | ((Additional form)) GFP-A Use Survival2 (Invitrogen) or equivalent to amplify this clone. Please use Schedule A (GFP-A) additionally. |
[open/close]| Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
|---|---|---|---|
| RDB06133 | pDUAL-HFG1c | DNA solution |
Electronic file
| Electronic file | Sequence RDB06133z.seq from Depositor |
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Featured content
| Featured content | Expression Vector Backbone of Schizosaccharomyces pombe (English text) |
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References
| original | Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162. |
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User reports and related articles (go to bottom)
RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.
Data are summerized on test sheet below.
| Test sheet | RDB12153_A3Gq.pdf |
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Nucleotide sequence of a portion of this resource (if available).
| Primer: nmt1Fw | RDB12153_A3Gqa.seq |
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>06133_12153_A3Gq_nmt1Fw_E03_15.ab1 1 ATGGATAATT TTTTAAATCA AGGGAAAACG TAACTCTCGG CTACTGGATG GTTCAGTCAC 61 CCAACGATTA CTGGGGAGAG AAAACAGGGC AAAAGCAAAG CTTAAAGGAA TCCGATTGTC 121 ATTCGGCAAT GTGCAGCGAA ACTAAAAACC GGATAATGGA CCTGTTAATC GAAACATTGA 181 AGATATATAA AGGAAGAGGA ATCCTGGCAT ATCATCAATT GAATAAGTTG AATTAATTAT 241 TTCAATCTCA TTCTCACTTT CTGACTTATA GTCGCTTTGT TAAAGCTAGC GTCGACAGGC 301 CTGGATCCTA TGCATCACCA CCATCATCAT ATGGATTATA AGGATGATGA CGATAAACCC 361 ATGGGTAAAG GAGAAGAACT TTTCACTGGA GTTGTCCCAA TTCTTGTTGA ATTAGATGGT 421 GATGTTAATG GGCACAAATT TTCTGTCAGT GGAGAGGGTG AAGGTGATGC AACATACGGA 481 AAACTTACCC TTAAATTTAT TTGCACTACT GGAAAACTAC CTGTTCCGTG GCCAACACTT 541 GTCACTACTT TCTGTTATGG TGTTCAATGC TTTTCAAGAT ACCCAGATCA CATGAAACAG 601 CATGACTTTT TCAAGAGTGC CATGCCCGAA GGCTATGTAC AGGAAAGAAC TATATTTTTC 661 AAAGATGACG GGAACTACAA GACACGTGCT GAAGTCAAGT TTGAAGGTGA TACCCTTGTT 721 AATAGAATCG AGTTAAAAGG TATTGATTTT AAAGAAGATG GAAACATTCT TGGACACAAA 781 TTGGAATACA ACTATAACTC ACACAATGTA TACATCATGG CAGACAAACA AAAGAATGGA 841 ATCAAAGTTA ACTTCAAAAT TAGACACAAC ATTGAAGATG GAAGCGTTCA ACTAGCAGAC 901 CATTATCAAC AAAATACTCC AATTGGCGAT GGCCCTGTCC TTTTACCAGA CAACCATTAC 961 CTGTCCACAC AATCTGCCCT TTCGAAAGAT CCCAACGAAA AGAGAGACCA CATGGTCCTT 1021 CTTGAGTTTG TAACAGCTGC TGGGATTACA CATGGCATGG ATGAACTATA CAAAGATATC 1081 ACAAGTTTGT ACAAAAAGCT GAACGAGAAA CGTTAAAATG ATATAATATC ATATTATTAA 1141 TTTAGATTTT GCATAAACCG GACTACTAAT AACTGTAACA CAACCATATC CAGTCACTTA 1201 TTGGGCGGGC GCCATTTAAG GAAACACTC // |
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| Primer: ADH_rev | RDB12153_A3Gqb.seq |
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>06133_12153_A3Gq_ADHrev_F03_18.ab1 1 GAAGGGAATT ACTAAAGAAT AAGAATTTTC GTTTTAAAAC CTAAGAGGTC ACTTTAAAAT 61 TTGTATACAC TTATTTTTTT TATAACTTAT TTAATAATAA AAATCATAAA TCATAAGAAA 121 TTCGCTTATT TAGAAGTGGC GCGCCGGATC CTCTAGAGTC GAGATCTCAT GATATCACCA 181 CTTTGTACAA GAAAGCTGAA CGAGAAACGT AAAATGATAT AAATATCAAT ATATTAAATT 241 AGATTTTGCA TAAAAAACAG ACTACATAAT ACTGTAAAAC ACAACATATC CAGTCACTAT 301 GGTCGACCTG CAGACTGGCT GTGTATAAGG GAGCCTGACA TTTATATTCC CCAGAACATC 361 AGGTTAATGG CGTTTTTGAT GTCATTTTCG CGGTGGCTGA GATCAGCCAC TTCTTCCCCG 421 ATAACGGAGA CCGGCACACT GGCCATATCG GTGGTCATCA TGCGCCAGCT TTCATCCCCG 481 ATATGCACCA CCGGGTAAAG TTCACGGGAG ACTTTATCTG ACAGCAGACG TGCACTGGCC 541 AGGGGGATCA CCATCCGTCG CCCGGGCGTG TCAATAATAT CACTCTGTAC ATCCACAAAC 601 AGACGATAAC GGCTCTCTCT TTTATAGGTG TAAACCTTAA ACTGCATTTC ACCAGCCCCT 661 GTTCTCGTCA GCAAAAGAGC CGTTCATTTC AATAAACCGG GCGACCTCAG CCATCCCTTC 721 CTGATTTTCC GCTTTCCAGC GTTCGGCACG CAGACGACGG GCTTCATTCT GCATGGTTGT 781 GCTTACCAGA CCGGAGATAT TGACATCATA TATGCCTTGA GCAACTGATA GCTGTCGCTG 841 TCAACTGTCA CTGTAATACG CTGCTTCATA GCATACCTCT TTTTGACATA CTTCGGGTAT 901 ACATATCAGT ATATATTCTT ATACCGCAAA AATCAGCGCG CAAATACGCA TACTGTTATC 961 TGGCTTTTAG TAAGCCGGAT CCACGCGTTT ACGCCCCGCC CTGCCACTCA TCGCAGTACT 1021 GTTGTAATTC ATTAAGCATT CTGCCGACAT GGCAGCCATC ACAAACGGCA TGATGACCCT 1081 GAATTCGCCA GCGGCCATCA GCACTGTCGC TTGCGTAATA AATATTGCCA TGTGAACCGG 1141 GGGGCGAAGA GTTGTTCATA TTTGGGCACA CGGTTTTAAA TCTCAGA // |
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Please visit Sequencing and PCR primers for primer information.
References
2018.12.10
GNP_filter3_RDBDEP_html_181012.pl