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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pCMFlag_hsFOSL2

Expression vector of human Fra2 (FOSL2).

Catalog number RDB06065
Resource name pCMFlag_hsFOSL2
Alternative name SET-0038_10
Clone info. Expression vector of human Fra2 (FOSL2). Expression was confirmed by Western blotting with anti-FLAG antibody.
Comment PCR template:HeLa cells cDNA. dT,Random primer mix. PCR cloning, forward primer: atgtaccaggattatcccgg; reverse primer: ttacagagccagcagagtgg.
Vector backbone pCMV_S-FLAG (Plasmid)
Size of vector backbone 5.5 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Human Fra2; FOSL2 cDNA
Depositor|Developer DNA Bank, |
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External Database
Human Fra2; FOSL2

          Reference sequence
            

          Distribution information

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          RDB06065 pCMFlag_hsFOSL2 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Sequence RDB06065z.seq
          Remarks, protocol and/or map (pdf) RDB06065.pdf

          Original reference

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content pCMV_tag and pRSV_tag assay-ready clones (English text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB18653_B1Aip1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV_Forward
          Sequence file: RDB18653_B1Aia.seq check
          >06065_18653_B1Ai_CMV_Forward_D02_11_ABI24.ab1
              1 NNNNNNNNNN NNCNNTTGNN GCNNTGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA
             61 GCAGAGCTCT CTGGCTAACT AGAGAACCCA CTGCTTACTG GCTTATCGAA ATTAATACGA
            121 CTCACTATAG GGAGACCCAA GCTGAATTCA CCATGGACTA CAAGGACGAT GACGATAAGG
            181 CGGCCATGTA CCAGGATTAT CCCGGGAACT TTGACACCTC GTCCCGGGGC AGCAGCGGCT
            241 CTCCTGCGCA CGCCGAGTCC TACTCCAGCG GCGGCGGCGG CCAGCAGAAA TTCCGGGTAG
            301 ATATGCCTGG CTCAGGCAGT GCATTCATCC CCACCATCAA CGCCATCACG ACCAGCCAGG
            361 ACCTGCAGTG GATGGTGCAG CCCACAGTGA TCACCTCCAT GTCCAACCCA TACCCTCGCT
            421 CGCACCCCTA CAGCCCCCTG CCGGGCCTGG CCTCTGTCCC TGGACACATG GCCCTCCCAA
            481 GACCTGGCGT GATCAAGACC ATTGGCACCA CCGTGGGCCG CAGGAGGAGA GATGAGCAGC
            541 TGTCTCCTGA AGAGGAGGAG AAGCGTCGCA TCCGGCGGGA GAGGAACAAG CTGGCTGCAG
            601 CCNAGTGCCG GANCCGACGC CNGGANCTGA NAGANAANCT GCNNGCGGAN ACAGANGANC
            661 TGGANGANGA NAANTCNNNC TGCNNANNNN ANTGNTGANC TGCNNNNNNN NANNNANANN
          721 CTNNNNNNNN NN
          //
          Primer: bGH_rev3
          Sequence file: RDB18653_B1Aib.seq check
          >06065_18653_B1Ai_bGH_rev3_E02_14_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN GNNGGNNNNN NNCAGATGGC TGGCAACTAG AAGGCACAGT
             61 CGAGGCTGAT CAGCGAGCTC TAGCATTTAG GTGACACTAT AGAATAGGGC CCTCTAGATG
            121 CATGCTCGAG CGGCCTTACA GAGCCAGCAG AGTGGGGGAG TTCAAGGAGT CTGATGATTG
            181 GTCCCCGCTG CTACTGCTTC TGCGGTGAGC CTTGGAGCAG GATTCGGAGG GAGATGCGGG
            241 TGACTCCTGC TCCAGGACGC TAGGATAGGT GAAGACGAGG TTCGAGGTGC CCGGAGTGAC
            301 AGCAGGTGTG GAGGTCACCA CGATGGGGGT GTGCAGGGGC TCCTCACCGT AGAAGCCCCC
            361 AGCAATGCTG ATGGGCTTGA TGACAGAGCG CTGGGCCTTG TCCAGCCCCG CCGACGAGGA
            421 CGAGGGGCTG TCCTCTTCCA GGGGCTCCTG TTTCACCACT ACAGCGCCCA CCGAGCCACC
            481 CCCACTGCGC ATGGGCTGCA GCCCAGGGGC TGGGGGCGAT CGGCGCTCCT CGGGGCTAAT
            541 CTTGCACACT GGGCCGTGAG CCACCAACAT GAACTCCAGC TTCTCCTTCT CCTTCTGCAG
            601 CTCANCAATC TCCTTCTGCA NGCCTGACTT CTCCTCCTCC AGCTCCTCTG TCTCCNCCTG
            661 CAGCTTCTCT GTCAGCTCCC GGCGTCNGNT CCGGNACTTG GNNGCNNCNN GCTNGNNCNN
          721 NNNCNNNNNN NNNN
          //
          Primer: SV40pro_ori_F
          Sequence file: RDB18653_B1Aic.seq check
          >06065_18653_B1Ai_SV40pro_ori_F_F02_17_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNCGC CTCNGCCTCT GNNCTATTCC NGAAGTAGTG AGGAGGCTTT
             61 TTTGGAGGCC TAGGCTTTTG CAAAAAGCTC CCGGGAGCTT GGATATCCAT TTTCGGATCT
            121 GATCAAGAGA CAGGATGAGG ATCGTTTCGC ATGATTGAAC AAGATGGATT GCACGCAGGT
            181 TCTCCGGCCG CTTGGGTGGA GAGGCTATTC GGCTATGACT GGGCACAACA GACAATCGGC
            241 TGCTCTGATG CCGCCGTGTT CCGGCTGTCA GCGCAGGGGC GCCCGGTTCT TTTTGTCAAG
            301 ACCGACCTGT CCGGTGCCCT GAATGAACTG CAGGACGAGG CAGCGCGGCT ATCGTGGCTG
            361 GCCACGACGG GCGTTCCTTG CGCAGCTGTG CTCGACGTTG TCACTGAAGC GGGAAGGGAC
            421 TGGCTGCTAT TGGGCGAAGT GCCGGGGCAG GATCTCCTGT CATCTCACCT TGCTCCTGCC
            481 GAGAAAGTAT CCATCATGGC TGATGCAATG CGGCGGCTGC ATACGCTTGA TCCGGCTACC
            541 TGCCCATTCG ACCACCAAGC GAAACATCGC ATCGAGCGAG CACGTACTCG GANGGAAGCC
            601 GGTCTTGTCN ATCAGGANGA NCTGGACNAA GAGCNTCANG GGCTCGCGNC NGCCNAACTG
            661 NTCNCCAGGC TCANNNNGCG CATGNCCNAN GGCNANGNNC TCNNCNNGNN CNNNNGNNNN
          721 NGNCNGNTNN NNNNNNNN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles


          2022.05.18

          GNP_filter3_RDBDEP_html_220516.pl