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pCMFlag_hsFOSL2 (#RDB06065)

Expression vector of human Fra2 (FOSL2).

Alternative name SET-0038_10
Clone info. Expression vector of human Fra2 (FOSL2). Expression was confirmed by Western blotting with anti-FLAG antibody.
Comment PCR template：HeLa cells cDNA. dT,Random primer mix. PCR cloning, forward primer: atgtaccaggattatcccgg; reverse primer: ttacagagccagcagagtgg.
Vector backbone pCMV_S-FLAG (RDB05956) (Plasmid)
Size of vector backbone 5.5 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Human Fra2; FOSL2 cDNA
Depositor|Developer DNA Bank, |
 
Sequence RDB06065z.seq
Remarks, protocol and/or map (pdf) RDB06065.pdf

Distribution information

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RDB06065 pCMFlag_hsFOSL2 DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pCMFlag_hsFOSL2 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB06065).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB18653_B1Aip1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward
Sequence file: RDB18653_B1Aia.seq check
>06065_18653_B1Ai_CMV_Forward_D02_11_ABI24.ab1
    1 NNNNNNNNNN NNCNNTTGNN GCNNTGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA
   61 GCAGAGCTCT CTGGCTAACT AGAGAACCCA CTGCTTACTG GCTTATCGAA ATTAATACGA
  121 CTCACTATAG GGAGACCCAA GCTGAATTCA CCATGGACTA CAAGGACGAT GACGATAAGG
  181 CGGCCATGTA CCAGGATTAT CCCGGGAACT TTGACACCTC GTCCCGGGGC AGCAGCGGCT
  241 CTCCTGCGCA CGCCGAGTCC TACTCCAGCG GCGGCGGCGG CCAGCAGAAA TTCCGGGTAG
  301 ATATGCCTGG CTCAGGCAGT GCATTCATCC CCACCATCAA CGCCATCACG ACCAGCCAGG
  361 ACCTGCAGTG GATGGTGCAG CCCACAGTGA TCACCTCCAT GTCCAACCCA TACCCTCGCT
  421 CGCACCCCTA CAGCCCCCTG CCGGGCCTGG CCTCTGTCCC TGGACACATG GCCCTCCCAA
  481 GACCTGGCGT GATCAAGACC ATTGGCACCA CCGTGGGCCG CAGGAGGAGA GATGAGCAGC
  541 TGTCTCCTGA AGAGGAGGAG AAGCGTCGCA TCCGGCGGGA GAGGAACAAG CTGGCTGCAG
  601 CCNAGTGCCG GANCCGACGC CNGGANCTGA NAGANAANCT GCNNGCGGAN ACAGANGANC
  661 TGGANGANGA NAANTCNNNC TGCNNANNNN ANTGNTGANC TGCNNNNNNN NANNNANANN
721 CTNNNNNNNN NN
//
Primer: bGH_rev3
Sequence file: RDB18653_B1Aib.seq check
>06065_18653_B1Ai_bGH_rev3_E02_14_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN GNNGGNNNNN NNCAGATGGC TGGCAACTAG AAGGCACAGT
   61 CGAGGCTGAT CAGCGAGCTC TAGCATTTAG GTGACACTAT AGAATAGGGC CCTCTAGATG
  121 CATGCTCGAG CGGCCTTACA GAGCCAGCAG AGTGGGGGAG TTCAAGGAGT CTGATGATTG
  181 GTCCCCGCTG CTACTGCTTC TGCGGTGAGC CTTGGAGCAG GATTCGGAGG GAGATGCGGG
  241 TGACTCCTGC TCCAGGACGC TAGGATAGGT GAAGACGAGG TTCGAGGTGC CCGGAGTGAC
  301 AGCAGGTGTG GAGGTCACCA CGATGGGGGT GTGCAGGGGC TCCTCACCGT AGAAGCCCCC
  361 AGCAATGCTG ATGGGCTTGA TGACAGAGCG CTGGGCCTTG TCCAGCCCCG CCGACGAGGA
  421 CGAGGGGCTG TCCTCTTCCA GGGGCTCCTG TTTCACCACT ACAGCGCCCA CCGAGCCACC
  481 CCCACTGCGC ATGGGCTGCA GCCCAGGGGC TGGGGGCGAT CGGCGCTCCT CGGGGCTAAT
  541 CTTGCACACT GGGCCGTGAG CCACCAACAT GAACTCCAGC TTCTCCTTCT CCTTCTGCAG
  601 CTCANCAATC TCCTTCTGCA NGCCTGACTT CTCCTCCTCC AGCTCCTCTG TCTCCNCCTG
  661 CAGCTTCTCT GTCAGCTCCC GGCGTCNGNT CCGGNACTTG GNNGCNNCNN GCTNGNNCNN
721 NNNCNNNNNN NNNN
//
Primer: SV40pro_ori_F
Sequence file: RDB18653_B1Aic.seq check
>06065_18653_B1Ai_SV40pro_ori_F_F02_17_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNCGC CTCNGCCTCT GNNCTATTCC NGAAGTAGTG AGGAGGCTTT
   61 TTTGGAGGCC TAGGCTTTTG CAAAAAGCTC CCGGGAGCTT GGATATCCAT TTTCGGATCT
  121 GATCAAGAGA CAGGATGAGG ATCGTTTCGC ATGATTGAAC AAGATGGATT GCACGCAGGT
  181 TCTCCGGCCG CTTGGGTGGA GAGGCTATTC GGCTATGACT GGGCACAACA GACAATCGGC
  241 TGCTCTGATG CCGCCGTGTT CCGGCTGTCA GCGCAGGGGC GCCCGGTTCT TTTTGTCAAG
  301 ACCGACCTGT CCGGTGCCCT GAATGAACTG CAGGACGAGG CAGCGCGGCT ATCGTGGCTG
  361 GCCACGACGG GCGTTCCTTG CGCAGCTGTG CTCGACGTTG TCACTGAAGC GGGAAGGGAC
  421 TGGCTGCTAT TGGGCGAAGT GCCGGGGCAG GATCTCCTGT CATCTCACCT TGCTCCTGCC
  481 GAGAAAGTAT CCATCATGGC TGATGCAATG CGGCGGCTGC ATACGCTTGA TCCGGCTACC
  541 TGCCCATTCG ACCACCAAGC GAAACATCGC ATCGAGCGAG CACGTACTCG GANGGAAGCC
  601 GGTCTTGTCN ATCAGGANGA NCTGGACNAA GAGCNTCANG GGCTCGCGNC NGCCNAACTG
  661 NTCNCCAGGC TCANNNNGCG CATGNCCNAN GGCNANGNNC TCNNCNNGNN CNNNNGNNNN
721 NGNCNGNTNN NNNNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles