Please review the QC test results indicated by 
icon below as well as clone information before placing your order.
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Please review the QC test results indicated by icon below as well as clone information before placing your order.
icon below as well as clone information before placing your order.
pCMFlag_hsFOS (#RDB06064)
Expression vector of human FOS.
| Alternative name | SET-0035_5 |
|---|---|
| Clone info. | Expression vector of human FOS. Expression was confirmed by Western blotting with anti-FLAG antibody. |
| Comment | PCR template：HeLa cells cDNA. dT,Random primer mix. PCR cloning, forward primer: atgatgttctcgggcttcaacg; reverse primer: tcacagggccagcagcgtgg. |
| Vector backbone | pCMV_S-FLAG (RDB05956) (Plasmid) |
| Size of vector backbone | 5.5 kb |
| Selectable markers | Amp^r (E. coli), Neo^r (mammalian cell) |
| Gene/insert name | Human FOS cDNA |
| Depositor|Developer | DNA Bank, | |
| Sequence | RDB06064z.seq | Remarks, protocol and/or map (pdf) | RDB06064.pdf |
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Distribution information
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Ordering forms | Order form [Credit Card Payment  ] [Bank Transfer Payment] [Example of order form ]MTA, for use for not-for-profit academic purpose [Word ] [Example of MTA ]Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. |
|---|---|
| Terms and conditions for distribution | The RECIPIENT agrees to describe RIKEN BRC as the source of the MATERIAL in any publication. |
[open/close]| 必要書類 | 提供依頼書 [依頼書の記入例 ]提供同意書 (MTA, 非営利学術目的用)[Word ] [MTAの記入例 ]手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 |
|---|---|
| MTAに書く使用条件 | 発表に際し、理研BRCから入手したことを明記すること。 |
| Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
|---|---|---|---|
| RDB06064 | pCMFlag_hsFOS | DNA solution |
icon below as well as clone information before placing your order.How to cite this biological resource
Materials & Methods section:
| The pCMFlag_hsFOS was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB06064). |
Reference section:
Further references such as user reports and related articles (go to bottom)
QC test results
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
| Test sheet | RDB18652_B1Aip1-1.pdf |
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Nucleotide sequence of a portion of this resource (if available).
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Primer: CMV_Forward (Pr0016) Region: CMV pro,FLAG,insert 5' Sequence file: RDB18652_B1Aia.seq
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>06064_18652_B1Ai_1_CMV_Forward_D01_10_ABI24.ab1 1 NNNNNNNNNC NNNNNNNNNN NNNGCNNTGG GCGGTAGGCG TGTACGGTGG GAGGTCTATA 61 TAAGCAGAGC TCTCTGGCTA ACTAGAGAAC CCACTGCTTA CTGGCTTATC GAAATTAATA 121 CGACTCACTA TAGGGAGACC CAAGCTGAAT TCACCATGGA CTACAAGGAC GATGACGATA 181 AGGCGGCCAT GATGTTCTCG GGCTTCAACG CAGACTACGA GGCGTCATCC TCCCGCTGCA 241 GCAGCGCGTC CCCGGCCGGG GATAGCCTCT CTTACTACCA CTCACCCGCA GACTCCTTCT 301 CCAGCATGGG CTCGCCTGTC AACGCGCAGG ACTTCTGCAC GGACCTGGCC GTCTCCAGTG 361 CCAACTTCAT TCCCACGGTC ACTGCCATCT CGACCAGTCC GGACCTGCAG TGGCTGGTGC 421 AGCCCGCCCT CGTCTCCTCT GTGGCCCCAT CGCAGACCAG AGCCCCTCAC CCTTTCGGAG 481 TCCCCGCCCC CTCCGCTGGG GCTTACTCCA GGGCTGGCGT TGTGAAGACC ATGACAGGAG 541 GCCGAGCGCA GAGCATTGGC AGGAGGGGCA AGGTGGANCN GTTATCTCCN GAAGAAGAAG 601 ANAAAAGGAG AATCCGAANG NAAAGGAATN AGATGGCTGC NGCCNANTGN CGCNACCGGA 661 NGANGGAGCT GANTGANACA CTNCNAGNGG ANANNGACCN NCTANAANNN GANAANNCTG 721 NNTTNNNNNN NNN // |
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Primer: bGH_rev3 (Pr0885) Region: bGHpA,insert 3' Sequence file: RDB18652_B1Aib.seq
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>06064_18652_B1Ai_1_bGH_rev3_E01_13_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNG NNNGGNNNNN NACAGATGGC TGGCAACTAG AAGGCACAGT 61 CGAGGCTGAT CAGCGAGCTC TAGCATTTAG GTGACACTAT AGAATAGGGC CCTCTAGATG 121 CATGCTCGAG CGGCCTCACA GGGCCAGCAG CGTGGGTGAG CTGAGCGAGT CAGAGGAAGG 181 CTCATTGCTG CTGCTGCCCT TGCGGTGGGC AGCTGCACAG CTGGGGAAGG AGTCAGCCTC 241 GGGGTAGGTG AAGACGAAGG AAGACGTGTA AGCAGTGCAG CTGGGAGTAC AGGTGACCAC 301 CGGAGTGCAC AGGGGCTCCA GCTCTGTGGC CATGGGCCCC ATCCCCAGGG AGCCACTGTG 361 CAGAGGCTCC CAGTCTGCTG CATAGAAGGA CCCAGATAGG TCCATGTCTG GCACGGAGCG 421 GGCTGTCTCA GAGCCACTGG GCCTGGATGA TGCTGGGAAC AGGAAGTCAT CAAAGGGCTC 481 GGTCTTCAGC TCCATGCTGC TGATGCTCTT GACAGGTTCC ACTGAGGGCT TGGGCTCAGG 541 GTCATTGAGG AGAGGCAGGG TGAAGGCCTC CTCAGACTCC GGGGTGGCAA CCTCTGGCAN 601 GCCCCCAGTC NNATCAAGGG AAGCCACAGA CATCTCTTCT GGGAAGCCNA NGTCATCNNG 661 NNTCTTGCAN GNANGNCNGT GANCTGNCNN GANGNNNTCT ANTTTTTNNN NCTNNNNNNN 721 CAGGNNNNNN NNNN // |
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Primer: SV40pro_ori_F (Pr0731) Region: SV40 pro_ori,NeoR Sequence file: RDB18652_B1Aic.seq
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>06064_18652_B1Ai_1_SV40pro_ori_F_F01_16_ABI24.ab1 1 NNNNNNNNNN NNNNGCCGCC TCNGCCTCTG NNCTATTCNN GAAGTAGTGA GGAGGCTTTT 61 TTGGAGGCCT AGGCTTTTGC AAAAAGCTCC CGGGAGCTTG GATATCCATT TTCGGATCTG 121 ATCAAGAGAC AGGATGAGGA TCGTTTCGCA TGATTGAACA AGATGGATTG CACGCAGGTT 181 CTCCGGCCGC TTGGGTGGAG AGGCTATTCG GCTATGACTG GGCACAACAG ACAATCGGCT 241 GCTCTGATGC CGCCGTGTTC CGGCTGTCAG CGCAGGGGCG CCCGGTTCTT TTTGTCAAGA 301 CCGACCTGTC CGGTGCCCTG AATGAACTGC AGGACGAGGC AGCGCGGCTA TCGTGGCTGG 361 CCACGACGGG CGTTCCTTGC GCAGCTGTGC TCGACGTTGT CACTGAAGCG GGAAGGGACT 421 GGCTGCTATT GGGCGAAGTG CCGGGGCAGG ATCTCCTGTC ATCTCACCTT GCTCCTGCCG 481 AGAAAGTATC CATCATGGCT GATGCAATGC GGCGGCTGCA TACGCTTGAT CCGGCTACCT 541 GCCCATTCGA CCACCAAGCG AAACATCGCA TCGAGCGAGC ACGTACTCGG ATGGAAGCCG 601 GTCTTGTCGA TCAGGANGAT CTGGACGAAN AGCATCANGG GCTCGCGNCN GCCGAACTGN 661 TCGCCAGGCT CAAGGCGCNN NNGNCCGANN GCNNNNANCT CNTCNNGNNC NNNGGCNANN 721 NNNGNTNNNN NANNNNNNN // |
Please visit 
Sequencing and PCR primers for primer information.
References
Original, user report and related articles
Resource search
Link
Other resources in our bankExternal Database
Human FOS
Reference sequence
Tips
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