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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pCMFlag_hsFOS

Expression vector of human FOS.

Catalog number RDB06064
Resource name pCMFlag_hsFOS
Alternative name SET-0035_5
Clone info. Expression vector of human FOS. Expression was confirmed by Western blotting with anti-FLAG antibody.
Comment PCR template:HeLa cells cDNA. dT,Random primer mix. PCR cloning, forward primer: atgatgttctcgggcttcaacg; reverse primer: tcacagggccagcagcgtgg.
Vector backbone pCMV_S-FLAG (Plasmid)
Size of vector backbone 5.5 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Human FOS cDNA
Depositor|Developer DNA Bank, |
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External Database
Human FOS

          Reference sequence
            

          Distribution information

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          RDB06064 pCMFlag_hsFOS DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Sequence RDB06064z.seq
          Remarks, protocol and/or map (pdf) RDB06064.pdf

          Original reference

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content pCMV_tag and pRSV_tag assay-ready clones (English text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB18652_B1Aip1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV_Forward
          Region: CMV pro,FLAG,insert 5'
          Sequence file: RDB18652_B1Aia.seq check
          >06064_18652_B1Ai_1_CMV_Forward_D01_10_ABI24.ab1
              1 NNNNNNNNNC NNNNNNNNNN NNNGCNNTGG GCGGTAGGCG TGTACGGTGG GAGGTCTATA
             61 TAAGCAGAGC TCTCTGGCTA ACTAGAGAAC CCACTGCTTA CTGGCTTATC GAAATTAATA
            121 CGACTCACTA TAGGGAGACC CAAGCTGAAT TCACCATGGA CTACAAGGAC GATGACGATA
            181 AGGCGGCCAT GATGTTCTCG GGCTTCAACG CAGACTACGA GGCGTCATCC TCCCGCTGCA
            241 GCAGCGCGTC CCCGGCCGGG GATAGCCTCT CTTACTACCA CTCACCCGCA GACTCCTTCT
            301 CCAGCATGGG CTCGCCTGTC AACGCGCAGG ACTTCTGCAC GGACCTGGCC GTCTCCAGTG
            361 CCAACTTCAT TCCCACGGTC ACTGCCATCT CGACCAGTCC GGACCTGCAG TGGCTGGTGC
            421 AGCCCGCCCT CGTCTCCTCT GTGGCCCCAT CGCAGACCAG AGCCCCTCAC CCTTTCGGAG
            481 TCCCCGCCCC CTCCGCTGGG GCTTACTCCA GGGCTGGCGT TGTGAAGACC ATGACAGGAG
            541 GCCGAGCGCA GAGCATTGGC AGGAGGGGCA AGGTGGANCN GTTATCTCCN GAAGAAGAAG
            601 ANAAAAGGAG AATCCGAANG NAAAGGAATN AGATGGCTGC NGCCNANTGN CGCNACCGGA
            661 NGANGGAGCT GANTGANACA CTNCNAGNGG ANANNGACCN NCTANAANNN GANAANNCTG
          721 NNTTNNNNNN NNN
          //
          Primer: bGH_rev3
          Region: bGHpA,insert 3'
          Sequence file: RDB18652_B1Aib.seq check
          >06064_18652_B1Ai_1_bGH_rev3_E01_13_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNG NNNGGNNNNN NACAGATGGC TGGCAACTAG AAGGCACAGT
             61 CGAGGCTGAT CAGCGAGCTC TAGCATTTAG GTGACACTAT AGAATAGGGC CCTCTAGATG
            121 CATGCTCGAG CGGCCTCACA GGGCCAGCAG CGTGGGTGAG CTGAGCGAGT CAGAGGAAGG
            181 CTCATTGCTG CTGCTGCCCT TGCGGTGGGC AGCTGCACAG CTGGGGAAGG AGTCAGCCTC
            241 GGGGTAGGTG AAGACGAAGG AAGACGTGTA AGCAGTGCAG CTGGGAGTAC AGGTGACCAC
            301 CGGAGTGCAC AGGGGCTCCA GCTCTGTGGC CATGGGCCCC ATCCCCAGGG AGCCACTGTG
            361 CAGAGGCTCC CAGTCTGCTG CATAGAAGGA CCCAGATAGG TCCATGTCTG GCACGGAGCG
            421 GGCTGTCTCA GAGCCACTGG GCCTGGATGA TGCTGGGAAC AGGAAGTCAT CAAAGGGCTC
            481 GGTCTTCAGC TCCATGCTGC TGATGCTCTT GACAGGTTCC ACTGAGGGCT TGGGCTCAGG
            541 GTCATTGAGG AGAGGCAGGG TGAAGGCCTC CTCAGACTCC GGGGTGGCAA CCTCTGGCAN
            601 GCCCCCAGTC NNATCAAGGG AAGCCACAGA CATCTCTTCT GGGAAGCCNA NGTCATCNNG
            661 NNTCTTGCAN GNANGNCNGT GANCTGNCNN GANGNNNTCT ANTTTTTNNN NCTNNNNNNN
          721 CAGGNNNNNN NNNN
          //
          Primer: SV40pro_ori_F
          Region: SV40 pro_ori,NeoR
          Sequence file: RDB18652_B1Aic.seq check
          >06064_18652_B1Ai_1_SV40pro_ori_F_F01_16_ABI24.ab1
              1 NNNNNNNNNN NNNNGCCGCC TCNGCCTCTG NNCTATTCNN GAAGTAGTGA GGAGGCTTTT
             61 TTGGAGGCCT AGGCTTTTGC AAAAAGCTCC CGGGAGCTTG GATATCCATT TTCGGATCTG
            121 ATCAAGAGAC AGGATGAGGA TCGTTTCGCA TGATTGAACA AGATGGATTG CACGCAGGTT
            181 CTCCGGCCGC TTGGGTGGAG AGGCTATTCG GCTATGACTG GGCACAACAG ACAATCGGCT
            241 GCTCTGATGC CGCCGTGTTC CGGCTGTCAG CGCAGGGGCG CCCGGTTCTT TTTGTCAAGA
            301 CCGACCTGTC CGGTGCCCTG AATGAACTGC AGGACGAGGC AGCGCGGCTA TCGTGGCTGG
            361 CCACGACGGG CGTTCCTTGC GCAGCTGTGC TCGACGTTGT CACTGAAGCG GGAAGGGACT
            421 GGCTGCTATT GGGCGAAGTG CCGGGGCAGG ATCTCCTGTC ATCTCACCTT GCTCCTGCCG
            481 AGAAAGTATC CATCATGGCT GATGCAATGC GGCGGCTGCA TACGCTTGAT CCGGCTACCT
            541 GCCCATTCGA CCACCAAGCG AAACATCGCA TCGAGCGAGC ACGTACTCGG ATGGAAGCCG
            601 GTCTTGTCGA TCAGGANGAT CTGGACGAAN AGCATCANGG GCTCGCGNCN GCCGAACTGN
            661 TCGCCAGGCT CAAGGCGCNN NNGNCCGANN GCNNNNANCT CNTCNNGNNC NNNGGCNANN
          721 NNNGNTNNNN NANNNNNNN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles


          2022.05.18

          GNP_filter3_RDBDEP_html_220516.pl