Please review the QC test results indicated by icon below as well as clone information before placing your order.

pAdex NSE(3.3)-ClaI-SV40pA (#RDB06017)

Shuttle vector to generate rAd with neuron specific enolase (NSE) promoter (3.3kbp)

Clone info.	Shuttle vector to generate rAd. Inserted sequence is regulated under the control of neuron specific enolase (NSE) promoter (3.3kbp).
Comment	NSE promoter (3.3kbp)-ClaI-SV40pA fragment was cloned on ClaI site of pAdex1cw. (NSE: neuron specific enolase). Produce adenovirus by COS-TPC method.
Vector backbone	pAxcw (Cosmid, use packaging extracts for transforming E. coli host)
Selectable markers	Amp^r
Gene/insert name	Rat enolase 2, gamma
Depositor|Developer	Hashimoto, Mitsuhiro |

 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Approval form by depositor [Word] Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.
Terms and conditions for distribution	This material is provided with written consent of Depositor. Manuscripts for the publication shall be made through the discussion between Developer/Depositor and Recipient investigator.
Remarks	((Additional form)) Approval Form(FormD) Remember that you will be working with samples containing infectious virus. Please obtain written approval of the depositor.

提供案内 (日本国内) [open/close]

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB06017	pAdex NSE(3.3)-ClaI-SV40pA	DNA solution	JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by icon below as well as clone information before placing your order.

---

How to cite this biological resource

Materials & Methods section:

The pAdex NSE(3.3)-ClaI-SV40pA was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB06017).

Reference section:

Further references such as user reports and related articles (go to bottom)

---

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB13142_A5Br.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pAdex_F Sequence file: RDB13142_A5Bra.seq
>06017_13142_A5Br_pAdex_F_G10_19.ab1     1 CAGTTCGCCA CACTCGCAGG GTCTGCACCA TGGTGCGGGT CTCATCGTAC CTCAGCACCT    61 TCCAGATCCA TCGTCGCGAG TCGACAATCG AGCTTGATAT CGAATTCCTG CAGCCCGGGG   121 GATCCCAGAA CTCAGGAGGC AGAGGCAGGC AGATCTCTGA GTTTGAGGCC AGCCTGGTCT   181 GCAGAGTGAT TTTCAGGACA GCCAGGGCTA CACAGTAGAA GTCTGTCTTG GAAGGAAGGA   241 AGGAAGGAGA GAAGGAAGGA AGGAAGGAAG GAGAGAAGGA AGGAAGGAAG GAAAAAACTT   301 CAAGGCCAGA TGCTGTAGCA TATGCCTATA GTCTAGGGCT TGGAAGTCAA CGGCGGCAGG   361 AGAATTGATG CAAGTGTGAG GGAGAAGCCA GATCCATACG GTGCGTAGTT CTGTGCCAGC   421 CAGGGGTACA CAGTGGGATT CAGTCAACAG CACTAACAGT AACAAAAAAA CCAGATGTGT   481 GGCTCATACC TATAATTCCA GCACTCAGGA ACCCGAGGCA GGAGGATTTC TGTGAGATTG   541 AGGCCAGCCT GGACTGCAGA GCCAGACTCT GTCACACCCC TCCTCCACAC ACACACACAC   601 ACACACACAC ACACACACAC ACACACACAC ACACAAAGAT ACACACACAC AGAGACACAC   661 ACACACAGAG ACACACACAC AGAGACACAC ACGTGCGCGC ACGCACACAC ACAACATCAC   721 AACATGCACA CACGTGCTCG CACACACGCG CGCGCACGCA CACACACACG GAAGGAGGAA   781 GGAGGAAGGA GGAGATGGAT GAACACATGC AGGGTCAGAG GGATTTTTCT CTAAGCTCCT   841 CTTTTCTGAT GGACAATTCT TTCAATTTTT TTTTGTGGTT CTATCAATCA AACCCAGGGC   901 TGTGTGCATG TGAAATCTGT ACCCCATCAC TGAGCCCAAC ACAACGCCAG TAGAGCTCAG   961 AGTGAGGAGT GCTCCTTTGA GCCCAGGCAC CTATGGGAGA TCAGTCTGCA TACTTTAACA  1021 CTGGATTCAT AAATGTTCGA AACCACAGAG TTTTGGAAAG AGGACATTAC TACGGACTGC  1081 AGCCTTTTTA TTCAAGCCTG GGGGGGGCGT GCAATTCCAT CTAGCTCTGG GTTCCTTAAC 1141 TTCAAAGGAA GAGAACTCTC TAAACAACAA GATAC //
Primer: pJB8-Forward Sequence file: RDB13142_A5Brb.seq
>06017_13142_A5Br_pJB8-Forward_H10_22.ab1     1 GATGGGTCAT ATGATAATGA GGGGGTGGAG TTTGTGACGT GGCGCGGGGC GTGGGAACGG    61 GGCGGGTGAC GTAGTAGTGT GGCGGAAGTG TGATGTTGCA AGTGTGGCGG AACACATGTA   121 AGCGACGGAT GTGGCAAAAG TGACGTTTTT GGTGTGCGCC GGTGTACACA GGAAGTGACA   181 ATTTTCGCGC GGTTTTAGGC GGATGTTGTA GTAAATTTGG GCGTAACCGA GTAAGATTTG   241 GCCATTTTCG CGGGAAAACT GAATAAGAGG AAGTGAAATC TGAATAATTT TGTGTTACTC   301 ATAGCGCGTA ATATTTGTCT AGGGCCGCGG GGACTTTGAC CGTTTACGTG GAGACTCGCC   361 CAGGTGTTTT TCTCAGGTGT TTTCCGCGTT CCGGGTCAAA GTTGGCGTTT TATTATTATA   421 GTCAGCATCG GGTGGCGGCC GCTCTAGGAT CCAGACATGA TAAGATACAT TGATGAGTTT   481 GGACAAACCA CAACTAGAAT GCAGTGAAAA AAATGCTTTA TTTGTGAAAT TTGTGATGCT   541 ATTGCTTTAT TTGTAACCAT TATAAGCTGC AATAAACAAG TTAACAACAA CAATTGCATT   601 CATTTTATGT TTCAGGTTCA GGGGGAGGTG TGGGAGGTTT TTTAAAGCAA GTAAAACCTC   661 TACAAATGTG GTATGGCTGA TTATGATCTC TAGTCAAGGC ACTATACATC AAATATTCCT   721 TATTAACCCC TTTACAAATT AAAAAGCTAA AGGTACACAA TTTTTGAGCA TAGTTATTAA   781 TAGCAGACAC TCTATGCCTG TGTGGAGTAA GAAAAAACAG TATGTTATGA TTATAACTGT   841 TATGCCTACT TATAAAGGTT ACAGAATATT TTTCCATAAA TTTCTTGTAT AGCAGTGCAG   901 CTTTTTCCTT TGTGGTGTAA ATAGCAAGCA AGCAGAGTTC TATTACTAAC ACAGCATGAC   961 TCAAAAAACT TAGCATTCTG ACGGAAAGTC CTTGGGGTCT TCTACCTTCT CTCTTTTTTG  1021 GAGGAGTAGA ATGTTGGAGA GTCAGCAGTA GCCTCATCAA TCCACTACGA ATGGGCAGTT  1081 TCTTTCTGTA AGCAAAACAG GTTTTGCTCC TCAAGGCGAG TCATCACTTG CGTGCGCCAT 1141 CATTCAGGTT TCCTTACGAG TGTGGGGATG TA //
Primer: SV40polyA-R Sequence file: RDB13141_A5Brc.seq
1

Please visit Sequencing and PCR primers for primer information.

---

References

Original, user report and related articles

Resource search

Go!

Link

Other resources in our bank

• Rat enolase 2, gamma and ortholog

---

External Database
Rat enolase 2, gamma

• in KEGG Orthology
• in NCBI Gene

---

Reference sequence
  

Tips

Featured content

---