RDB06016 - pAdex CAG-SwaI-IRES-EGFP

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icon below as well as clone information before placing your order.

pAdex CAG-SwaI-IRES-EGFP (#RDB06016)

Shuttle vector to generate rAd with CAG expression unit.

Clone info.	Shuttle vector to generate rAd. Inserted sequence is regulated under the control of CAG expression unit.
Comment	SwaI-IRES-EGFP fragment (1.3kbp) was cloned on SwaI site of pAdex1CAw. IRES: internal ribosomal entry site, EGFP: enhanced green fluorecense protein. Produce adenovirus by COS-TPC method.
Vector backbone	pAxCAwt (Cosmid, use packaging extracts for transforming E. coli host)
Selectable markers	Amp^r
Growth conditions	37C, LB+Amp
Gene/insert name	Aequorea victoria GFP cDNA
Depositor\|Developer	Hashimoto, Mitsuhiro \|

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Approval form by depositor [Word]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.

Terms and conditions for distribution This material is provided with written consent of Depositor. Manuscripts for the publication shall be made through the discussion between Developer/Depositor and Recipient investigator.
Additional terms and conditions:
Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.

Remarks ((Additional form)) Approval Form(FormD)
Remember that you will be working with samples containing infectious virus.
Please obtain written approval of the depositor.

提供案内 (日本国内) [open/close]

Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Approval form by depositor [Word] Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.
Terms and conditions for distribution	This material is provided with written consent of Depositor. Manuscripts for the publication shall be made through the discussion between Developer/Depositor and Recipient investigator. Additional terms and conditions: Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.
Remarks	((Additional form)) Approval Form(FormD) Remember that you will be working with samples containing infectious virus. Please obtain written approval of the depositor.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 寄託者による提供承諾書 [Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件	寄託者に許可をとること。使用にあたっては、常に連絡をとり、発表等に関しても事前に合意を要する。付加的使用条件： CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学宮崎純一博士への謝辞の表明を必要とする。
備考	【追加提供依頼書】承諾書(FormD) このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。寄託者の書面による提供承諾が必要です。

Catalog #	Resource name	Availability	Shipping form	Fee (non-profit org.)
RDB06016	pAdex CAG-SwaI-IRES-EGFP	Under QC test. Please contact us.	DNA solution	JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge