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pAdex NSE(1.8K)-SwaI-SV40pA (#RDB06013)

Shuttle vector to generate rAd withneuron specific enolase (NSE) promoter (1.8kbp)

Clone info. Shuttle vector to generate rAd. Inserted sequence is regulated under the control of neuron specific enolase (NSE) promoter (1.8kbp).
Comment NSE promoter (1.8kbp)-SwaI-SV40-A fragment was cloned on SwaI site of pAdex1cw. (NSE: Neuron specific enolase) ClaI site (2653) can be used. Other ClaI sites cannot be digested because of methylation. Produce adenovirus by COS-TPC method.
Vector backbone pAxcw (Cosmid, use packaging extracts for transforming E. coli host)
Selectable markers Amp^r
Gene/insert name Rat enolase 2, gamma
Depositor|Developer Hashimoto, Mitsuhiro |
 

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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB06013 pAdex NSE(1.8K)-SwaI-SV40pA DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pAdex NSE(1.8K)-SwaI-SV40pA was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB06013).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB13139_A5Br.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pAdex_F
Sequence file: RDB13139_A5Bra.seq check
>06013_13139_A5Br_pAdex_F_A10_01.ab1
    1 CATGTTCGCA ACTCGCAGGG TCTGCACCAT GGTGCGGGTC TCATCGTACC TCAGCACCTT
   61 CCAGATCCAT CGTCGCGAGT CGACAATCGA TTTCGACGGT ATCGATAAGC TTGATATCGA
  121 ATTCCTGCAG CCCCCTCCTC TGCTCGCCCA ATCCTTCCAA CCCCCTATGG TGGTATGGCT
  181 GACACAGAAA ATGTCTGCTC CTGTATGGGA CATTTGCCCC TCTTCTCCAA ATATAAGACA
  241 GGATGAGGCC TAGCTTTTGC TGCTCCAAAG TTTTAAAAGA ACACATTGCA CGGCATTTAG
  301 GGACTCTAAA GGGTGGAGGA GGAATGAGGG AATTGCATCA TGCCAAGGCT GGTCCTCATC
  361 CATCACTGCT TCCAGGGCCC AGAGTGGCTT CCAGGAGGTA TTCTTACAAA GGAAGCCCGA
  421 TCTGTAGCTA ACACTCAGAG CCCATTTTCC TGCGTTAACC CCTCCCGACC TCATATACAG
  481 GAGTAACATG ATCAGTGACC TGGGGGAGCT GGCCAAACTG CGGGACCTGC CCAAGCTGAG
  541 GGCCTTGGTG CTGCTGGACA ACCCCTGTGC CGATGAGACT GACTACCGCC AGGAGGCCCT
  601 GGTGCAGATG GCACACCTAG AGCGCCTAGA CAAAGAGTAC TATGAGGACG AGGACCGGGC
  661 AGAAGCTGAG GAGATCCGAC AGAGGCTGAA GGAGGAACAG GAGCAAGAAC TCGACCCGGA
  721 CCAAGACATG GAACCGTACC TCCCGCCAAC TTAGTGGCTC CTCTAGCCTG CAGGGACAGT
  781 AAAGGTGATG GCAGGAAAGC AGCCCCCGGA GGTCAAAGGC TGGGCACGCG GGAGGAGAGG
  841 CCAGAGTCAG AGCTGCGGGT ATCTCAGATA TGAAAGAAAG ATGAGAGAGG CTCAGAAGAG
  901 GTAAGAAAAG ACACAAGAGA CCAGAGAACG GAGAAGAATT AGAGAGGGAG GCAGAGGACC
  961 GCTGTCTCTA CAGACATAGC TGGGTAGAGA CTGGGAGGAA GGGATGAACC TGAGCGCATG
 1021 ATGGTATGAG TGGCTGTGGT ATATGAGGAT GTAGCTGGTC CAGGAAGATC TGCACTAAAT
 1081 CTGAAGCCTA AATTACAGAC CCGGGTTGGA TAGCCACGAG GTCCGATGAG TCAGGCACTC
1141 GAAGGCACTA TG
//
Primer: pJB8-Forward
Sequence file: RDB13139_A5Brb.seq check
>06013_13139_A5Br_pJB8-Forward_B10_04.ab1
    1 GACTGGCATA TGATATGAGG GGGTGGAGTT TGTGACGTGG CGCGGGGCGT GGGAACGGGG
   61 CGGGTGACGT AGTAGTGTGG CGGAAGTGTG ATGTTGCAAG TGTGGCGGAA CACATGTAAG
  121 CGACGGATGT GGCAAAAGTG ACGTTTTTGG TGTGCGCCGG TGTACACAGG AAGTGACAAT
  181 TTTCGCGCGG TTTTAGGCGG ATGTTGTAGT AAATTTGGGC GTAACCGAGT AAGATTTGGC
  241 CATTTTCGCG GGAAAACTGA ATAAGAGGAA GTGAAATCTG AATAATTTTG TGTTACTCAT
  301 AGCGCGTAAT ATTTGTCTAG GGCCGCGGGG ACTTTGACCG TTTACGTGGA GACTCGCCCA
  361 GGTGTTTTTC TCAGGTGTTT TCCGCGTTCC GGGTCAAAGT TGGCGTTTTA TTATTATAGT
  421 CAGCATCGAT TTCTAGTGGA TCCAGACATG ATAAGATACA TTGATGAGTT TGGACAAACC
  481 ACAACTAGAA TGCAGTGAAA AAAATGCTTT ATTTGTGAAA TTTGTGATGC TATTGCTTTA
  541 TTTGTAACCA TTATAAGCTG CAATAAACAA GTTAACAACA ACAATTGCAT TCATTTTATG
  601 TTTCAGGTTC AGGGGGAGGT GTGGGAGGTT TTTTAAAGCA AGTAAAACCT CTACAAATGT
  661 GGTATGGCTG ATTATGATCT CTAGTCAAGG CACTATACAT CAAATATTCC TTATTAACCC
  721 CTTTACAAAT TAAAAAGCTA AAGGTACACA ATTTTTGAGC ATAGTTATTA ATAGCAGACA
  781 CTCTATGCCT GTGTGGAGTA AGAAAAAACA GTATGTTATG ATTATAACTG TTATGCCTAC
  841 TTATAAAGGT TACAGAATAT TTTTCCATAA TTTTCTTGTA TAGCAGTGCA GCTTTTTCCT
  901 TTGTGGTGTA AATAGCAAGC AAGCAGAGTT CTATTACTAA CACAGCATGA CTCAAAAAAC
  961 TTAGCATTCT GATGAAGTCT TGGGGTCTCT ACTTTCTCTC CTTTTTGAGA GTAGATGTGA
 1021 GAGTCAGCAG TAGCTCATCA TCACTAGAAT AGGCAGTTCT TCTGGAGGCA AACCAAGGAT
 1081 GTGTCTCATT AAGCAGTGCA CACATGCTCC ATCATCAGGT TCCATTAAGG TTGTGGGTTC
1141 TTTCA
//
Primer: SV40polyA-R
Sequence file: RDB13139_A5Brc.seq check
>06013_13139_A5Br_SV40poly_R_B12_06.ab1
    1 CTCAGTCAGT GAAGCAGTAA CCTCTACAAT GTGGTATGGC TGATTATGAT CTCTAGTCAA
   61 GGCACTATAC ATCAAATATT CCTTATTAAC CCCTTTACAA ATTAAAAAGC TAAAGGTACA
  121 CAATTTTTGA GCATAGTTAT TAATAGCAGA CACTCTATGC CTGTGTGGAG TAAGAAAAAA
  181 CAGTATGTTA TGATTATAAC TGTTATGCCT ACTTATAAAG GTTACAGAAT ATTTTTCCAT
  241 AATTTTCTTG TATAGCAGTG CAGCTTTTTC CTTTGTGGTG TAAATAGCAA AGCAAGCAAG
  301 AGTTCTATTA CTAAACACAG CATGACTCAA AAAACTTAGC AATTCTGAAG GAAAGTCCTT
  361 GGGGTCTTCT ACCTTTCTCT TCTTTTTTGG AGGAGTAGAA TGTTGAGAGT CAGCAGTAGC
  421 CTCATCATCA CTAGATGGCA TTTCTTCTGA GCAAAACAGG TTTTCCTCAT TAAAGGCATT
  481 CCACCACTGC TCCCATTCAT CAGTTCCATA GGTTGGAATC TAAAATACAC AAACAATTAG
  541 AATCAGTAGT TTAACACATT ATACACTTAA AAATTTTATA TTTACCTTAG AGCTTTAAAT
  601 CTCTGTAGGT AGTTTGTCCA ATTATGTCAC ACCACAGAAG TAAGGTTCCT TCACAAAGAT
  661 CCGATAAGCT TGATATCGAA TTCCTGCAGC CCGATTTAAA TGGGGGATCC CCCTCGAGGA
  721 CTGCAGACTC AGCCGGTGGC GGTGGCGGTG GTGACGGCGG CGGCGCAGAG AGAGCAGGAG
  781 TGGCGGCGGC TGCGGCTCCC AGCGCGGGCG GGGGGTGGGC AGGGGGCGGC GCCTATAGAG
  841 CCGAGCGAGC GCATGTGACC CGGGGGCCCC GATGAGTCAA GAGCTGCAGG CGGAGACGCA
  901 CGTACGAGCG CGGGTGGGTG GTGGGGAAAC CGAACGGGTG GGGGAAAGGG CCCGGGCCGA
  961 TCCAGGACCA AGCTGGGCAA GCCCATAAGT ACACGGGATG CTCATAACCT TGTTCAGCCC
 1021 AGCTCGCAGG GGAAAGCAGG CGTGCCCCGT ATATTCAGGT ATTATTGGGG CGGTGCGTAG
 1081 CACCCTACAC ACAGAGTGGC AGCGCAGCCG CCTACCGTGG GTGTCGACTG ACATCGCACT
 1141 CGAACCCATC GACACGACTG AAGATGAGCG CGACTCTTCA TTTTATTGTA AGCGCTAGAG
1201 CTCAGCGATT AGATGCTAGC ACAGATGTGA CA
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles