Resource data sheet
Prev.

Cox2-COE

Expression vector of mouse Cox2

Catalog number RDB05985
Resource name Cox2-COE
Clone info. Expression vector of mouse Cox2.
Vector backbone pCALwL (Plasmid)
Selectable markers Amp^r
Gene/insert name Mouse cyclooxygenase-2 cDNA
Depositor Herschman, Harvey |
 
Other clones in our bank mouse Ptgs2 (NCBI Gene 19225) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR Clones are provided with the written consent of the depositor and with acknowledgment of the depositor in any subsequent publication. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Genesis, 44, 177-182 (2006).)
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RDB05985 Cox2-COE DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Featured content

References

original Kamei, K., Transgenic mouse for conditional, tissue-specific Cox-2 overexpression. Genesis, 44, 177-182 (2006). PMID 16604526.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB13531_A5J1p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pMAMneo-Reverse RDB13531_A5J1a.seq
>05985_13531_A5J1_pMAMneo-Reverse_H12_24.ab1
    1 ATATAATATG TCACACCACA GAAGTAAGGT TCCTTCACAA AGATCCCTCG AGAAAAAAAA
   61 TATAAAAGAG ATGGAGGAAC GGGAAAAAGT TAGTTGTGGT GATAGGTGGC AAGTGGTATT
  121 CCGTAAGAAC AACAAGAAAA GCATTTCATA TTATGGCTGA ACTGAGCGAA CAAGTGCAAA
  181 ATTTAAGCAT CAACGACAAC AACGAGAATG GTTATGTTCC TCCTCACTTA AGAGGAAAAC
  241 CAAGAAGTGC CAGAAATAAC AGTAGCAACT ACAATAACAA CAACGGCGGC TACAACGGTG
  301 GCCGTGGCGG TGGCAGCTTC TTTAGCAACA ACCGTCGTGG TGGTTACGGC AACGGTGGTT
  361 TCTTCGGTGG AAACAACGGT GGCAGCAGAT CTAACGGCCG TTCTGGTGGT AGATGGATCG
  421 ATGGCAAACA TGTCCCAGCT CCAAGAAACG AAAAGGCCGA GATCGCCATA TTTGGTGTCC
  481 CCGAGGATCA TCGAATTCCT GCAGCCCCGA TAAGGATCAA ATCCGAACGC GTATAACTTC
  541 GTATAGCATA CATTATACGA AGTTATCTCG AGTCGCTCGG TACCTCGAGC TCAAGCTTCG
  601 AATTGATCCC ACCTCTGCGA TGCTCTTCCG AGCTGTGCTG CTCTGCGCTG CCCTGGGGCT
  661 CAGCCAGGCA GCAAATCCTT GCTGTTCCAA TCCATGTCAA AACCGTGGGG AATGTATGAG
  721 CACAGGATTT GACCAGTATA GTGTGACTGT ACCCGGACTG GATTCTATGT GAAACTGTAC
  781 TACACCTGAT TTCTGACAGA TCAATACTGC TGAAGCCCAC CCCAAACACA GTGCACTACA
  841 TCTGACCCAC TCAGGGAGTC TGAACATTGT GAACAACATC CCTTTCTGCA GTTAATCATG
  901 AAATGTGCTG ACTTCTGATC ATATTGATTG GCAGTTCACA TACTCAATGG CCATCGCTAC
961 AAGCTGACAG CCTTTCGCAA
//
Primer: IRES-R RDB13531_A5J1b.seq
>05985_13531_A5J1_IRES-R_D02_11.ab1
    1 CCTATCACCG GGCCTTGGCC AGTAACGTTA GGGGGGGGGG AGGGGAGAGG GGCGGATCCC
   61 GGGCCCGCGG TACCGTCGAC TGCAGAATTT CGAGTAGACT TTTACAGCTC AGTTGAACGC
  121 CTTTTGATTA GTACTGTAGG GTTAATGTCA TCTAGTCTGG AGTGGGAGGC ACTTGCATTG
  181 ATGGTGGCTG TTTTGGTAGG CTGTGGATCT TGCACATTGA AAGAAGTGAA GGGACACCCC
  241 TTCACATTAT TGCAGATGAG AGACTGAATT GAGGCAGTAT TGATGATCTT AAAACCCACT
  301 TCGCCTCCAA AGGTGCTCGG CTTCCAGTAT TGAGGAGAAC AGATGGGATT TCCCATAAGT
  361 CCTTTCAAGG AGAATGGTGC TCCAAGCTCT ACCATGGTCT CCCCAAAGAT AGCATCTGGA
  421 CGAGGTTTTT CCACCAGCAG GGCAGGGTAC AGTTCCATGA CATCGATGTC ACTGTAGAGG
  481 GCTTTCAATT CTGCAGCCAT TTCCTTCTCT CCTGTAAGTT CTTCAAATGA TGTGTACGGC
  541 TTCAGGGAGA AGCGTTTGCG GTACTCATTG AGAGACTGGT ATTTCATCTC TCTGCTCTGG
  601 TCAATGGAGG CCTTTGCCAC TGCTTGTACA GCAATTGGCA CATTTCTTCC CCCAGCAACC
  661 CGGCCAGCAA TCTGTCTGGT GAATGACTCA ACAAACTGAG TGAGTCCATG TTCCAGGAGG
  721 ATGGAGTTGT TGTAGAGAAA ACTGTTTGAA GCTGTACTCC TGGTCTTCAA TGTTGAAGGT
  781 GTCGGGGCAG CAGGGGGGTG CCCAGTGATA GAGTGTGTTG GAATTCAGAG GCAATGCGGT
  841 TTCTGATACT GGAACTGCTG GTTGAAAAAG GAGCTCTGGG GTCAAACTTG AGTTTGAAGT
  901 GGTAACCGCT CAGGTGTTTG CACGTAGTCT TCGATCACTA TCTTGAATAG TCATCTCCTA
  961 TGAAGTATGA AGTCTGCCTG GGTTTGGGAA ATAAGTTGGA TCAATCACCC TCACTTCAGG
 1021 GATGCTCCCT GCTTTGAGTA TGTCGCACAC ATCCTGCTGG TGCCTCCCCG AAGCCCAGAA
 1081 TGGTTGGGCA TACCATCAAT CAGACTCAGG CACCAGATCC AAAGAACTTT CCTGGCTCCC
 1141 CACAAGCAAA ACTGCAGGTT TCTTCAGGAT TGTGCAGGAA GGGTTAGAAT CCATTCCTCT
1201 CTACTTGATG TCTTTGAACC TGTTGAGAGG TTGCTGGTAC
//
Primer: IRES-F RDB13531_A5J1c.seq
>05985_13531_A5J1_IRES-F_C12_09.ab1
    1 GCAGTCGTTA ATGTGTTTAG TCGAGGTTAA AAAAACGTCT AGGCCCCCCG AACCACGGGG
   61 ACGTGGTTTT CCTTTGAAAA ACACGATGAT AATATGGCCA CAACCATGGC TTCCAAGGTG
  121 TACGACCCCG AGCAACGCAA ACGCATGATC ACTGGGCCTC AGTGGTGGGC TCGCTGCAAG
  181 CAAATGAACG TGCTGGACTC CTTCATCAAC TACTATGATT CCGAGAAGCA CGCCGAGAAC
  241 GCCGTGATTT TTCTGCATGG TAACGCTGCC TCCAGCTACC TGTGGAGGCA CGTCGTGCCT
  301 CACATCGAGC CCGTGGCTAG ATGCATCATC CCTGATCTGA TCGGAATGGG TAAGTCCGGC
  361 AAGAGCGGGA ATGGCTCATA TCGCCTCCTG GATCACTACA AGTACCTCAC CGCTTGGTTC
  421 GAGCTGCTGA ACCTTCCAAA GAAAATCATC TTTGTGGGCC ACGACTGGGG GGCTTGTCTG
  481 GCCTTTCACT ACTCCTACGA GCACCAAGAC AAGATCAAGG CCATCGTCCA TGCTGAGAGT
  541 GTCGTGGACG TGATCGAGTC CTGGGACGAG TGGCCTGACA TCGAGGAGGA TATCGCCCTG
  601 ATCAAGAGCG AAGAGGGCGA GAAAATGGTG CTTGAGAATA ACTTCTTCGT CGAGACCATG
  661 CTCCCAAGCA AGATCATGCG GAAACTGGAG CCTGAGGAGT TCGCTGCCTA CCTGGAGCCA
  721 TTCAAGGAGA AGGCGAGGTT AGACGGCCTA CCCTCTCCTG GCCCTCGCGA GATCCCTCTC
  781 GTTAAGGGAG GCAAGCCCGA CGTCGTCCAG ATTGTCCGCA ACTACAACGC CTACCTTCGG
  841 GCCAGCGACG ATCTGCCTAA GATGTTCATC GAGTCCGACC CTGGGTTCTT TTCCAACGCT
  901 ATTGTCGAGG GAGCTAAGAA GTTCCCTAAC ACCGAGTTCG TGAAGTGAAG GGCCTCCACT
  961 TCAGCCAGGA GGACGCTCCA GATGAATGGG TAGTACATCA GAGCTTCGTG GAGCGCGTGC
 1021 TGAGACGAGC AGTATTCTAG AGCGCGGGGA TCAATCATCA CTCCTCAGTG CAGCTGCTAT
 1081 CGAAGTGTGC TGGTGTGGCA TGCTGACTCC AATACACTGA ATCTTTTCCT TGCAAGTAGG
1141 GAACTCATGA CCCTTGAACA TCTGGACTTC GGCCTAATAA GGGCAATTAA TTTTC
//
Primer: pAxCA_R1 RDB13531_A5J1d.seq
>05985_13531_A5J1_pAxCA_R1_D12_12.ab1
    1 GGAGACAAGG CATTGGGCCA CACCAGCCAC CACCTTCTGA TAGGCAGCCT GCACCTGAGG
   61 AGTGAATTGA TTTGATCCCC GGCCGCTCTA GAATTACTGC TCGTTCTTCA GCACGCGCTC
  121 CACGAAGCTC TTGATGTACT TACCCATTTC ATCTGGAGCG TCCTCCTGGC TGAAGTGGAG
  181 GCCCTTCACC TTCACGAACT CGGTGTTAGG GAACTTCTTA GCTCCCTCGA CAATAGCGTT
  241 GGAAAAGAAC CCAGGGTCGG ACTCGATGAA CATCTTAGGC AGATCGTCGC TGGCCCGAAG
  301 GTAGGCGTTG TAGTTGCGGA CAATCTGGAC GACGTCGGGC TTGCCTCCCT TAACGAGAGG
  361 GATCTCGCGA GGCCAGGAGA GGGTAGGCCG TCTAACCTCG CCCTTCTCCT TGAATGGCTC
  421 CAGGTAGGCA GCGAACTCCT CAGGCTCCAG TTTCCGCATG ATCTTGCTTG GGAGCATGGT
  481 CTCGACGAAG AAGTTATTCT CAAGCACCAT TTTCTCGCCC TCTTCGCTCT TGATCAGGGC
  541 GATATCCTCC TCGATGTCAG GCCACTCGTC CCAGGACTCG ATCACGTCCA CGACACTCTC
  601 AGCATGGACG ATGGCCTTGA TCTTGTCTTG GTGCTCGTAG GAGTAGTGAA AGGCCAGACA
  661 AGCCCCCCAG TCGTGGCCCA CAAAGATGAT TTTCTTTGGA AGGTTCAGCA GCTCGAACCA
  721 AGCGGTGAGG TACTTGTAGT GATCCAGGAG GCGATATGAG CCATTCCCGC TCTTGCCGGA
  781 CTTACCCATT CCGATCAGAT CAGGGATGAT GCATCTAGCC ACGGGCTCGA TGTGAGGCAC
  841 GACGTGCCTC CACAGGTAGC TGGAGGCAGC GTTACCATGC AGAAAAATCA CGGCGTTCTC
  901 GGCGTGCTTC TCGGAATCAT AGTAGTTGAT GAAGGAGTCC AGCACGTTCA TTTGCTTGCA
  961 GCGAGCCCAC CACTGAGGCC CAGTGATCAT GCGTTTGCGT TGCTCGGGGT CGTACACTTG
 1021 AGCCATGATG TGGTATATTA TCATCGTGTT TTTCAAAGAA ACACGTCCCC GTGATCGGGG
 1081 GGCTAGACGA TTTTTAACTT CGACTTAATA CATGGTAAGC ATGTGTACCG AGCCCAGATT
1141 CAGATCCAAT
//
Primer: pAxCA_F1 RDB13531_A5J1e.seq
>05985_13531_A5J1_pAxCA_F1_A12_03.ab1
    1 TAAAGAAAAG AGGCTTCTAC ATGTTCATGC CTTCTTCTTT TTCCTACAGC TCCTGGGCAA
   61 CGTGCTGGTT ATTGTGCTGT CTCATCATTT TGGCAAAGAA TTGATTAATT CGAGCGAACG
  121 CGTATAACTT CGTATAGCAT ACATTATACG AAGTTATCTC GAGTCGGATT TGATCCACCG
  181 GTCGCCACCA TGGTGAGCAA GGGCGAGGAG CTGTTCACCG GGGTGGTGCC CATCCTGGTC
  241 GAGCTGGACG GCGACGTAAA CGGCCACAAG TTCAGCGTGT CCGGCGAGGG CGAGGGCGAT
  301 GCCACCTACG GCAAGCTGAC CCTGAAGTTC ATCTGCACCA CCGGCAAGCT GCCCGTGCCC
  361 TGGCCCACCC TCGTGACCAC CCTGACCTAC GGCGTGCAGT GCTTCAGCCG CTACCCCGAC
  421 CACATGAAGC AGCACGACTT CTTCAAGTCC GCCATGCCCG AAGGCTACGT CCAGGAGCGC
  481 ACCATCTTCT TCAAGGACGA CGGCAACTAC AAGACCCGCG CCGAGGTGAA GTTCGAGGGC
  541 GACACCCTGG TGAACCGCAT CGAGCTGAAG GGCATCGACT TCAAGGAGGA CGGCAACATC
  601 CTGGGGCACA AGCTGGAGTA CAACTACAAC AGCCACAACG TCTATATCAT GGCCGACAAG
  661 CAGAAGAACG GCATCAAGGT GAACTTCAAG ATCCGCCACA ACATCGAGGA CGGCAGCGTG
  721 CAGCTCGCCG ACCACTACCA GCAGAACACC CCCATCGGCG ACGGCCCCGT GCTGCTGCCC
  781 GACAACCACT ACCTGAGCAC CCAGTCCGCC CTGAGCAAAG ACCCCAACGA GAAGCGCGAT
  841 CACATGGTCC TGCTGGAGTT CGTGACCGCC GCCGGGATCA CTCTCGGCAT GGACGAGCTG
  901 TACAAGTAAG CGGCCGGGCC GCTTCGAGCA GACATGATAG ATACATTGAT GAGTTTGGAC
  961 AACCACACTA GATGCAGTGA AAAAATGCTT TATTTGTGAA TTTGTGATGC TATGCTTTAT
 1021 TTGTACATTA TAGGCTGCCA TTAACAGTAA CACCACATGC ATCATATGTC AGTCAGCAGA
1081 GGTGTGGAGT TTTAAGCCAG ATAACCTCTT AC
//
Primer: EGFP-C RDB13531_A5J1f.seq
>05985_13531_A5J1_EGFP-C_D09_12.ab1
    1 CCGTTCTCTC GGCATGGACG AGCTGTACAA GTAAAGCGGC CGGCCGCTTC GAGCAGACAT
   61 GATAAGATAC ATTGATGAGT TTGGACAAAC CACAACTAGA ATGCAGTGAA AAAAATGCTT
  121 TATTTGTGAA ATTTGTGATG CTATTGCTTT ATTTGTAACC ATTATAAGCT GCAATAAACA
  181 AGTTAACAAC AACAATTGCA TTCATTTTAT GTTTCAGGTT CAGGGGGAGG TGTGGGAGGT
  241 TTTTTAAAGC AAGTAAAACC TCTACAAATG TGGTAAAATC GAGCTTAGGA TCGAGCCCCA
  301 GCTGGTTCTT TCCGCCTCAG AAGCCATAGA GCCCACCGCA TCCCCAGCAT GCCTGCTATT
  361 GTCTTCCCAA TCCTCCCCCT TGCTGTCCTG CCCCACCCCA CCCCCCAGAA TAGAATGACA
  421 CCTACTCAGA CAATGCGATG CAATTTCCTC ATTTTATTAG GAAAGGACAG TGGGAGTGGC
  481 ACCTTCCAGG GTCAAGGAAG GCACGGGGGA GGGGCAAACA ACAGATGGCT GGCAACTAGA
  541 AGGCACAGTC GAGGCTGATC AGCGAGCTCT AGAGAATTGA TCCCCTCAGA AGAACTCGTC
  601 AAGAAGGCGA TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG GCGATACCGT AAAGCACGAG
  661 GAAGCGGTCA GCCCATTCGC CGCCAAGCTC TTCAGCAATA TCACGGGTAG CCAACGCTAT
  721 GTCCTGATAG CGGTCCGCCA CACCCAGCCG GCCACAGTCG ATGAATCCAG AAAAGCGGCC
  781 ATTTTCCACC ATGATATTCG GCAAGCAGGC ATCGCCATGG GTCACGACGA GATCATCGCC
  841 GTCGGGCATG CGCGCCTTGA GCCTGGCGAA CAGTTCGGCT GGCGCGAGCC CCTGATGCTC
  901 TTCGTCCAGA TCATCCTGAT CGACAAGACC GGCTTCCATC CGAGTACGTG CTCGCTCGAT
  961 GCGATGTTTC GCTTGGTGGT CGAATGGGCA GGTAGCCGGG ATCAAGCGTA TGCAGCCGCC
 1021 GCATTTGCAT CAGCCATGAT GGATACTTTC TCGGGCAGGA GCACGTTGAG ATGACCAGGA
1081 GATCTGTCCA GTCACTCGCC GATAGCCAGG TAGGTTCCGT TCCCGTCTTC AAGTTGACA
//
Primer: Neo-M_R RDB13531_A5J1g.seq
>05985_13531_A5J1_Neo-M_R_G07_19.ab1
    1 GGAGGGCCAG TAGCAGCCAG TCCTTCCCGC TTCAGTGACA ACGTCGAGCA CAGCTGCGCA
   61 AGGAACGCCC GTCGTGGCCA GCCACGATAG CCGCGCTGCC TCGTCCTGCA GTTCATTCAG
  121 GGCACCGGAC AGGTCGGTCT TGACAAAAAG AACCGGGCGC CCCTGCGCTG ACAGCCGGAA
  181 CACGGCGGCA TCAGAGCAGC CGATTGTCTG TTGTGCCCAG TCATAGCCGA ATAGCCTCTC
  241 CACCCAAGCG GCCGGAGAAC CTGCGTGCAA TCCATCTTGT TCAATGGCCG ATCCCATATT
  301 GGCTGCAGGT CGAAAGGCCC GGAGATGAGG AAGAGGAGAA CAGCGCGGCA GACGTGCGCT
  361 TTTGAAGCGT GCAGAATGCC GGGCCTCCGG AGGACCTTCG GGCGCCCGCC CCGCCCCTGA
  421 GCCCGCCCCT GAGCCCGCCC CCGGACCCAC CCCTTCCCAG CCTCTGAGCC CAGAAAGCGA
  481 AGGAGCAAAG CTGCTATTGG CCGCTGCCCC AAAGGCCTAC CCGCTTCCAT TGCTCAGCGG
  541 TGCTGTCCAT CTGCACGAGA CTAGTGAGAC GTGCTACTTC CATTTGTCAC GTCCTGCACG
  601 ACGCGAGCTG CGGGGCGGGG GGGAACTTCC TGACTAGGGG AGGAGTAGAA GGTGGCGCGA
  661 AGGGGCCACC AAAGAACGGA GCCGGTTGGC GCCTACCGGT GGATGTGGAA TGTGTGCGAG
  721 GCCAGAGGCC ACTTGTGTAG CGCCAAGTGC CCAGCGGGGC TGCTAAAGCG CATGCTCCAG
  781 ACTGCCTTGG GAAAAGCGCC TCCCCTACCC GGTAGAATTC GATAGCTTAC TTACCATGTC
  841 AGATCCAGAC ATGATAAGAT ACATTGATGA GTTTGGACAA ACCACAACTA GAATGCAGTG
  901 AAAAAAAATG CTTTATTTGT GAAATTTGTG ATGCTATTGC TTTATTTGTA ACCATTATAA
  961 GCTGCAATAA ACAAGTTAAC AACAACAATT GCATTCATTT TATGTTTCAG GTTCAGGGGA
 1021 GGTGTGGGAG GTTTTTTTAA AGCAAGTAAA ACCTCTACAA ATGTGTATGG GCTGATTATG
 1081 ATCTCTAGTC AAAGGCACTT ATACATCCAA TATCGTTATT TACCCCTTAC AAATTAAAAA
1141 GGCCTTAAAG GGTTAAC
//
Primer: PGK_N_rev RDB13531_A5J1h.seq
>05985_13531_A5J1_PGK_N_rev_H07_22.ab1
    1 GCGATTGGGA CGCTCCCCTA CCCGGTAGAT TCGATAGCTT ACTTACCATG TCAGATCCAG
   61 ACATGATAAG ATACATTGAT GAGTTTGGAC AAACCACAAC TAGAATGCAG TGAAAAAAAT
  121 GCTTTATTTG TGAAATTTGT GATGCTATTG CTTTATTTGT AACCATTATA AGCTGCAATA
  181 AACAAGTTAA CAACAACAAT TGCATTCATT TTATGTTTCA GGTTCAGGGG GAGGTGTGGG
  241 AGGTTTTTTA AAGCAAGTAA AACCTCTACA AATGTGGTAT GGCTGATTAT GATCTCTAGT
  301 CAAGGCACTA TACATCAAAT ATTCCTTATT AACCCCTTTA CAAATTAAAA AGCTAAAGGT
  361 ACACAATTTT TGAGCATAGT TATTAATAGC AGACACTCTA TGCCTGTGTG GAGTAAGAAA
  421 AAACAGTATG TTATGATTAT AACTGTTATG CCTACTTATA AAGGTTACAG AATATTTTTC
  481 CATAATTTTC TTGTATAGCA GTGCAGCTTT TTCCTTTGTG GTGTAAATAG CAAAGCAAGC
  541 AAGAGTTCTA TTACTAAACA CAGCATGACT CAAAAAACTT AGCAATTCTG AAGGAAAGTC
  601 CTTGGGGTCT TCTACCTTTC TCTTCTTTTT TGGAGGAGTA GAATGTTGAG AGTCAGCAGT
  661 AGCCTCATCA TCACTAGATG GCATTTCTTC TGAGCAAAAC AGGTTTTCCT CATTAAAGGC
  721 ATTCCACCAC TGCTCCCATT CATCAGTTCC ATAGGTTGGA AATCTAAAAT ACACAAACAA
  781 TTAGAATCAG TAGTTTAACA CATTTATACA CTTAAAAATT TTATATTTAC CCTTAGAGCT
  841 TTAAATCTCT GTAGGTAGTT TGTCCCAATA ATGTCACACA CAGAAGGTAA GTTCTTCACA
  901 AAAGATCCCC TCGAGAAAAA ATATTAAAGA GATGAGAACG GAAAAAGTAG TGTGGTGATA
  961 GGTGGCAGTG GTATTCCGTA GGACACAGAG CAATTTCATA TTTATGGCTG ACCTGAACCG
 1021 AACAATTGCC AATTAAGGCA TCAACGGACA ACACAGGATG TAGTCTCACT TAAGAACCCA
1081 AAAGTGCA
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Kamei, K., Transgenic mouse for conditional, tissue-specific Cox-2 overexpression. Genesis, 44, 177-182 (2006). PMID 16604526.

2018.11.21

GNP_filter3_RDBDEP_html_181012.pl