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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pGEX-MAZ-delta34 (#RDB05974)

Human MAZ cDNA

Clone info. Human MAZ cDNA. This clone contains a DNA fragment corresponding to the 152nt..1,760nt of NM_002383.4 (where its CDS is 174nt..1,607nt) with deletion of 3rd and 4th zinc finger (corresponding 1,077nt to 1,262nt was replaced with AGATCT). There is five alanine deletion corresponding to 1,493nt to 1,507nt.
Vector backbone pGEX-4T-2 (Plasmid)
Size of vector backbone 4.97 kb
Selectable markers Amp^r
Gene/insert name Human MAZ cDNA
Depositor|Developer Yokoyama, Kazunari | Song, Jun |
 

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (J. Biol. Chem., 276, 30429-30434 (2001)).
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手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (J. Biol. Chem., 276, 30429-30434 (2001))。

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RDB05974 pGEX-MAZ-delta34 DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pGEX-MAZ-delta34 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB05974).

Reference section:

Song, J., Ugai, H., Ogawa, K., Wang, Y., Sarai, A., Obata, Y., Kanazawa, I., Sun, K., Itakura, K., Yokoyama, K.K., Two consecutive zinc fingers in Sp1 and in MAZ are essential for interactions with cis-elements. J. Biol. Chem., 276, 30429-30434 (2001). PMID 11395515. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB19169_B1F4p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pGEX-F2 (Pr0403)
Region: GST,Thrombin site,insert 5'
Sequence file: RDB19169_B1F4a.seq check
>05974_19169_B1F4_3_pGEX-F2_F01_06_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNTATTGNNG CTATCCCNCA AATTGATAAG TACTTGAAAT
   61 CCAGCAAGTA TATAGCATGG CCTTTGCAGG GCTGGCAAGC CACGTTTGGT GGTGGCGACC
  121 ATCCTCCAAA ATCGGATCTG GTTCCGCGTG GATCCCCAGG AATTCTGCAG TCGACGGTAC
  181 CGGGCCCCGC TGCGGCCGAG GCCATGTTCC CGGTGTTTCC TTGCACGCTG CTGGCCCCCC
  241 CCTTCCCCGT GCTGGGCCTG GACTCCCGGG GGGTGGGCGG CCTCATGAAC TCCTTCCCGC
  301 CACCTCAGGG TCACGCCCAG AACCCCCTGC AGGTCGGGGC TGAGCTCCAG TCCCGCTTCT
  361 TTGCCTCCCA GGGCTGCGCC CAGAGTCCAT TCCAGGCCGC GCCGGCGCCC CCGCCCACGC
  421 CCCAGGCCCC GGCGGCCGAG CCCCTCCAGG TGGACTTGCT CCCGGTGCTC GCCGCCGCCC
  481 AGGAGTCCGC CGCGGCTGCT GCGGCCGCTG CCGCCGCTGC TGCCGCCGTC GCTGCCGCGC
  541 CCCCGGCCCC TGCCGCCGCC TCTACGGTGG ACACAGCGGC CCTGAAGCAG CCTCCGGCGC
  601 CCCCTCCGCC ACCCCCGCCA GTGTCNGCGC CCGCGGCCGA NGCCGCGCCC CCCGCCTNCG
  661 CCGCCACTAT CGCCGCGGNN GCNNNNANCG NNGNCNTANC CCCNNNCNCN ANNNNCNNNN
721 NNNNNCNNNN NNNNNN
//
Primer: pGEX-RSEQ (Pr0077)
Region: insert 3'
Sequence file: RDB19169_B1F4b.seq check
>05974_19169_B1F4_3_pGX-RSEQ_G01_07_ABI08.ab1
    1 NNNNNNNNAN NNCGCGNNGN ANANCGTCAG TCAGTCACGA TGCGGCCGCT CGAGTCGACC
   61 CGGGAATTCC CCTAAGAAAA CATTTCTTCC TCCTTTCCTT CTGGAGGCTC CTTGGTTGGT
  121 AGGGAAATAG GAGTTGGTGG GAAAAGAGGG GGGAGAGGAG CTTGTACCAA GGGACTCTAC
  181 TCCATTCTCC CCTCTCCCCC GCAACCAACT TGGAGCTCAC CAGGGTTGGG AGGGAAGTGG
  241 CTGAGAGCTC ACAGGCACCC CCTCCGCCCC CGAGAGGGAG CCCACAGCTG TGGGAGGGGC
  301 TGCTACTGCT GCCGCTGCTG CCGCTGCCGC CGCCATTGGA CAAACCTCAC CAGTACCTTT
  361 GTTGCAGAGC TCACAGACAT GGTGAGGACC CTGGCTGTGC ACCTTCATGT GGTCCGAAAT
  421 ATAAGCCGAG CTCAGCATCT TGCCACACAC GTGACATGGC ACTTTCTCCT CGTGTCGTAC
  481 TGTGTGCGCC CGCAGCCGAT CCTTCGTGGC GAAAGCTGCC TCACATTTCT CACATTTGAA
  541 GGGCCGAGAT CTGTGCGACA GCTTGTGTCG GTTCANGTGG TAGACGTCGC GGAANGNCTT
  601 GCCACACATC TCGCANGCAT GGTTCTTCCG GANGCGCTTC CCCGANGCGG TCGNGGTCAC
  661 NNCGCCACCN GNNGNNACTG NNGNNGCNNN NNNNNNGNNN CNNNNTNTCC CCNNNCCCNN
721 NNNNNNNN
//
Primer: MAZ-M_R (Pr0151)
Region: insert mid
Sequence file: RDB19169_B1F4c.seq check
>05974_19169_B1F4F_3_MAZ_MR_A05_01_ABI08.ab1
    1 NNNNNNNNNC CNNNNNNNNN NNNNNNNNNN NNNGTNNGCN NNNNNNNNNN ANGCCGCGCC
   61 CCCCGCCTCC GCCGCCACTA TCGCCGCGGC GGCGGCCACC GCCGTCGTAG CCCCAACCTC
  121 GACGGTCGCC GTGGCCCCGG TCGCGTCTGC CTTGGAGAAG AAGACAAAGA GCAAGGGGCC
  181 CTACATCTGC GCTCTGTGCG CCAAGGAGTT CAAGAACGGC TACAATCTCC GGAGGCACGA
  241 AGCCATCCAC ACGGGAGCCA AGGCCGGCCG GGTCCCCTCG GGTGCTATGA AGATGCCGAC
  301 CATGGTGCCC CTGAGCCTCC TGAGCGTGCC CCAGCTGAGC GGAGCCGGCG GGGGAGGGGG
  361 AGAGGCGGGT GCCGGCGGCG GCGCTGCCGC AGTGGCCGCC GGTGGCGTGG TGACCACGAC
  421 CGCCTCGGGG AAGCGCATCC GGAAGAACCA TGCCTGCGAG ATGTGTGGCA AGGCCTTCCG
  481 CGACGTCTAC CACCTGAACC GACACAAGCT GTCGCACAGA TCTCGGCCCT TCAAATGTGA
  541 GAAATGTGAG GCAGCTTTCG CCACGAAGGA TCGGCTGCGG GCGCNNNNNG TACGACNCNA
  601 NGAGAAAGTG CCATGTCACG TGTGTGGCAA GATGCTGAGC TCGGCTTATA TTTCGGACCA
  661 CATGAAGGTG CACAGCCAGG GTCCTCACCN TGNNNGNGAG CTCTGCNANC AAAGGTACTG
  721 GTGAGGNTTG TCNNATGGGC GGCGGNNNNN NNNNCAGCGG CAGCAGTAGC ANCCCCTCCC
  781 ACAGCTGTGG GCTCCCCTCN NCGGGGGCGG ANGGGGGTGC CTGNGANCTC TCAGCCACTT
  841 CCCTCCCANC CCTGGGNGNA GNTCCANNTT GGTTGCNGGG GGNANANGGG GANNATGGNA
  901 GNANANNCCC TTGGTACANN CTCCTNNNCC CCCCCTCTTT TTCCNNNNAN NTCNNNTTTN
  961 NCNNNNNNAA NNNANNNNNN CCNNNNNANN NNAANGGANG ANNNNNNNGN TTTCNTNNNG
1021 GNANNNCCNN GGNNNNNNNN NN
//
Primer: hMAZ-M_R (Pr1025)
Region: insert mid
Sequence file: RDB19169_B1F4d.seq check
>05974_19169_B1F4_hMaz-M_R_G06_07_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNTGCCTC NNNTTTCTCN CATTTGAAGG GCCGAGATCT
   61 GTGCGACAGC TTGTGTCGGT TCAGGTGGTA GACGTCGCGG AAGGCCTTGC CACACATCTC
  121 GCAGGCATGG TTCTTCCGGA TGCGCTTCCC CGAGGCGGTC GTGGTCACCA CGCCACCGGC
  181 GGCCACTGCG GCAGCGCCGC CGCCGGCACC CGCCTCTCCC CCTCCCCCGC CGGCTCCGCT
  241 CAGCTGGGGC ACGCTCAGGA GGCTCAGGGG CACCATGGTC GGCATCTTCA TAGCACCCGA
  301 GGGGACCCGG CCGGCCTTGG CTCCCGTGTG GATGGCTTCG TGCCTCCGGA GATTGTAGCC
  361 GTTCTTGAAC TCCTTGGCGC ACAGAGCGCA GATGTAGGGC CCCTTGCTCT TTGTCTTCTT
  421 CTCCAAGGCA GACGCGACCG GGGCCACGGC GACCGTCGAG GTTGGGGCTA CGACGGCGGT
  481 GGCCGCCGCC GCGGCGATAG TGGCGGCGGA GGCGGGGGGC GCGGCCTCGG CCGCGGGCGC
  541 CGACACTGGC GGGGGTGGCG GAGGGGGCGC CGGAGGCTGC TTCAGGGCCG CTGTGTCCAC
  601 CGTAGAGGCG GCGGCAGGGG CCGGGGGCGC GGCAGCGACG GCGGCAGCAG CGGCGGCAGC
  661 GGCCGCAGCA GCCGCGGCGG ACTCCTGGGC GGCGGCGAGC ACCGGGAGCA AGTCCACCTG
  721 GAGGGGCTCG GCCGCCGGGG CCTGGNGCGT GGGCNNNNNG CCGGCGCGGC CTGNAATGGA
  781 CTCTGGGCGC AGCCCTGGGA NGCAAAGAAG CGGGANTGGA NCTCANNNNN ACCTGCAGGG
  841 GGTTCTGGGC GTGANCCTGA NGTGGCGGGA AGGAGTTCAT GANGCCGCCC ANCCCCCGGG
  901 ANNNCNGGNC CANCACGGGG AANNGGGGGG GGNCAGCANC GNGCANNNNA ACNNCNGGNA
  961 NNNTGGNNNC NGNCNNCANN NNGGNCCNNN ANNGNNNNNT GNNNANNTNN NGGGNNNCCN
1021 NNCNNNNNCN NNNNNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Song, J., Two consecutive zinc fingers in Sp1 and in MAZ are essential for interactions with cis-elements. J. Biol. Chem., 276, 30429-30434 (2001). PMID 11395515. [link to RRC of NBRP]
user_report Wang H.Y., Transcriptional regulation of human eosinophil RNases by an evolutionary- conserved sequence motif in primate genome. BMC Mol. Biol., 8:89 (2007). PMID 17927842. [link to RRC of NBRP]