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pGEX-MAZ-delta34

Human MAZ cDNA

Catalog number RDB05974
Resource name pGEX-MAZ-delta34
Clone info. Human MAZ cDNA. This clone contains a DNA fragment corresponding to the 152nt..1,760nt of NM_002383.4 (where its CDS is 174nt..1,607nt) with deletion of 3rd and 4th zinc finger (corresponding 1,077nt to 1,262nt was replaced with AGATCT). There is five alanine deletion corresponding to 1,493nt to 1,507nt.
Vector backbone pGEX-4T-2 (Plasmid)
Size of vector backbone 4.97 kb
Selectable markers Amp^r
Gene/insert name Human MAZ cDNA
Depositor|Developer Yokoyama, Kazunari | Song, Jun |
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External Database
Human MAZ

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (J. Biol. Chem., 276, 30429-30434 (2001)).
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (J. Biol. Chem., 276, 30429-30434 (2001))。
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          RDB05974 pGEX-MAZ-delta34 DNA solution

          Ordering Information [in Japanese] [in English]
          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Song, J., Two consecutive zinc fingers in Sp1 and in MAZ are essential for interactions with cis-elements. J. Biol. Chem., 276, 30429-30434 (2001). PMID 11395515.

          Further references such as user reports and related articles (go to bottom)

          Featured content


          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB19169_B1F4p1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pGEX-F2
          Region: GST,Thrombin site,insert 5'
          Sequence file: RDB19169_B1F4a.seq check
          >05974_19169_B1F4_3_pGEX-F2_F01_06_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNTATTGNNG CTATCCCNCA AATTGATAAG TACTTGAAAT
             61 CCAGCAAGTA TATAGCATGG CCTTTGCAGG GCTGGCAAGC CACGTTTGGT GGTGGCGACC
            121 ATCCTCCAAA ATCGGATCTG GTTCCGCGTG GATCCCCAGG AATTCTGCAG TCGACGGTAC
            181 CGGGCCCCGC TGCGGCCGAG GCCATGTTCC CGGTGTTTCC TTGCACGCTG CTGGCCCCCC
            241 CCTTCCCCGT GCTGGGCCTG GACTCCCGGG GGGTGGGCGG CCTCATGAAC TCCTTCCCGC
            301 CACCTCAGGG TCACGCCCAG AACCCCCTGC AGGTCGGGGC TGAGCTCCAG TCCCGCTTCT
            361 TTGCCTCCCA GGGCTGCGCC CAGAGTCCAT TCCAGGCCGC GCCGGCGCCC CCGCCCACGC
            421 CCCAGGCCCC GGCGGCCGAG CCCCTCCAGG TGGACTTGCT CCCGGTGCTC GCCGCCGCCC
            481 AGGAGTCCGC CGCGGCTGCT GCGGCCGCTG CCGCCGCTGC TGCCGCCGTC GCTGCCGCGC
            541 CCCCGGCCCC TGCCGCCGCC TCTACGGTGG ACACAGCGGC CCTGAAGCAG CCTCCGGCGC
            601 CCCCTCCGCC ACCCCCGCCA GTGTCNGCGC CCGCGGCCGA NGCCGCGCCC CCCGCCTNCG
            661 CCGCCACTAT CGCCGCGGNN GCNNNNANCG NNGNCNTANC CCCNNNCNCN ANNNNCNNNN
          721 NNNNNCNNNN NNNNNN
          //
          Primer: pGEX-RSEQ
          Region: insert 3'
          Sequence file: RDB19169_B1F4b.seq check
          >05974_19169_B1F4_3_pGX-RSEQ_G01_07_ABI08.ab1
              1 NNNNNNNNAN NNCGCGNNGN ANANCGTCAG TCAGTCACGA TGCGGCCGCT CGAGTCGACC
             61 CGGGAATTCC CCTAAGAAAA CATTTCTTCC TCCTTTCCTT CTGGAGGCTC CTTGGTTGGT
            121 AGGGAAATAG GAGTTGGTGG GAAAAGAGGG GGGAGAGGAG CTTGTACCAA GGGACTCTAC
            181 TCCATTCTCC CCTCTCCCCC GCAACCAACT TGGAGCTCAC CAGGGTTGGG AGGGAAGTGG
            241 CTGAGAGCTC ACAGGCACCC CCTCCGCCCC CGAGAGGGAG CCCACAGCTG TGGGAGGGGC
            301 TGCTACTGCT GCCGCTGCTG CCGCTGCCGC CGCCATTGGA CAAACCTCAC CAGTACCTTT
            361 GTTGCAGAGC TCACAGACAT GGTGAGGACC CTGGCTGTGC ACCTTCATGT GGTCCGAAAT
            421 ATAAGCCGAG CTCAGCATCT TGCCACACAC GTGACATGGC ACTTTCTCCT CGTGTCGTAC
            481 TGTGTGCGCC CGCAGCCGAT CCTTCGTGGC GAAAGCTGCC TCACATTTCT CACATTTGAA
            541 GGGCCGAGAT CTGTGCGACA GCTTGTGTCG GTTCANGTGG TAGACGTCGC GGAANGNCTT
            601 GCCACACATC TCGCANGCAT GGTTCTTCCG GANGCGCTTC CCCGANGCGG TCGNGGTCAC
            661 NNCGCCACCN GNNGNNACTG NNGNNGCNNN NNNNNNGNNN CNNNNTNTCC CCNNNCCCNN
          721 NNNNNNNN
          //
          Primer: MAZ-M_R
          Region: insert mid
          Sequence file: RDB19169_B1F4c.seq check
          >05974_19169_B1F4F_3_MAZ_MR_A05_01_ABI08.ab1
              1 NNNNNNNNNC CNNNNNNNNN NNNNNNNNNN NNNGTNNGCN NNNNNNNNNN ANGCCGCGCC
             61 CCCCGCCTCC GCCGCCACTA TCGCCGCGGC GGCGGCCACC GCCGTCGTAG CCCCAACCTC
            121 GACGGTCGCC GTGGCCCCGG TCGCGTCTGC CTTGGAGAAG AAGACAAAGA GCAAGGGGCC
            181 CTACATCTGC GCTCTGTGCG CCAAGGAGTT CAAGAACGGC TACAATCTCC GGAGGCACGA
            241 AGCCATCCAC ACGGGAGCCA AGGCCGGCCG GGTCCCCTCG GGTGCTATGA AGATGCCGAC
            301 CATGGTGCCC CTGAGCCTCC TGAGCGTGCC CCAGCTGAGC GGAGCCGGCG GGGGAGGGGG
            361 AGAGGCGGGT GCCGGCGGCG GCGCTGCCGC AGTGGCCGCC GGTGGCGTGG TGACCACGAC
            421 CGCCTCGGGG AAGCGCATCC GGAAGAACCA TGCCTGCGAG ATGTGTGGCA AGGCCTTCCG
            481 CGACGTCTAC CACCTGAACC GACACAAGCT GTCGCACAGA TCTCGGCCCT TCAAATGTGA
            541 GAAATGTGAG GCAGCTTTCG CCACGAAGGA TCGGCTGCGG GCGCNNNNNG TACGACNCNA
            601 NGAGAAAGTG CCATGTCACG TGTGTGGCAA GATGCTGAGC TCGGCTTATA TTTCGGACCA
            661 CATGAAGGTG CACAGCCAGG GTCCTCACCN TGNNNGNGAG CTCTGCNANC AAAGGTACTG
            721 GTGAGGNTTG TCNNATGGGC GGCGGNNNNN NNNNCAGCGG CAGCAGTAGC ANCCCCTCCC
            781 ACAGCTGTGG GCTCCCCTCN NCGGGGGCGG ANGGGGGTGC CTGNGANCTC TCAGCCACTT
            841 CCCTCCCANC CCTGGGNGNA GNTCCANNTT GGTTGCNGGG GGNANANGGG GANNATGGNA
            901 GNANANNCCC TTGGTACANN CTCCTNNNCC CCCCCTCTTT TTCCNNNNAN NTCNNNTTTN
            961 NCNNNNNNAA NNNANNNNNN CCNNNNNANN NNAANGGANG ANNNNNNNGN TTTCNTNNNG
          1021 GNANNNCCNN GGNNNNNNNN NN
          //
          Primer: hMAZ-M_R
          Region: insert mid
          Sequence file: RDB19169_B1F4d.seq check
          >05974_19169_B1F4_hMaz-M_R_G06_07_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNTGCCTC NNNTTTCTCN CATTTGAAGG GCCGAGATCT
             61 GTGCGACAGC TTGTGTCGGT TCAGGTGGTA GACGTCGCGG AAGGCCTTGC CACACATCTC
            121 GCAGGCATGG TTCTTCCGGA TGCGCTTCCC CGAGGCGGTC GTGGTCACCA CGCCACCGGC
            181 GGCCACTGCG GCAGCGCCGC CGCCGGCACC CGCCTCTCCC CCTCCCCCGC CGGCTCCGCT
            241 CAGCTGGGGC ACGCTCAGGA GGCTCAGGGG CACCATGGTC GGCATCTTCA TAGCACCCGA
            301 GGGGACCCGG CCGGCCTTGG CTCCCGTGTG GATGGCTTCG TGCCTCCGGA GATTGTAGCC
            361 GTTCTTGAAC TCCTTGGCGC ACAGAGCGCA GATGTAGGGC CCCTTGCTCT TTGTCTTCTT
            421 CTCCAAGGCA GACGCGACCG GGGCCACGGC GACCGTCGAG GTTGGGGCTA CGACGGCGGT
            481 GGCCGCCGCC GCGGCGATAG TGGCGGCGGA GGCGGGGGGC GCGGCCTCGG CCGCGGGCGC
            541 CGACACTGGC GGGGGTGGCG GAGGGGGCGC CGGAGGCTGC TTCAGGGCCG CTGTGTCCAC
            601 CGTAGAGGCG GCGGCAGGGG CCGGGGGCGC GGCAGCGACG GCGGCAGCAG CGGCGGCAGC
            661 GGCCGCAGCA GCCGCGGCGG ACTCCTGGGC GGCGGCGAGC ACCGGGAGCA AGTCCACCTG
            721 GAGGGGCTCG GCCGCCGGGG CCTGGNGCGT GGGCNNNNNG CCGGCGCGGC CTGNAATGGA
            781 CTCTGGGCGC AGCCCTGGGA NGCAAAGAAG CGGGANTGGA NCTCANNNNN ACCTGCAGGG
            841 GGTTCTGGGC GTGANCCTGA NGTGGCGGGA AGGAGTTCAT GANGCCGCCC ANCCCCCGGG
            901 ANNNCNGGNC CANCACGGGG AANNGGGGGG GGNCAGCANC GNGCANNNNA ACNNCNGGNA
            961 NNNTGGNNNC NGNCNNCANN NNGGNCCNNN ANNGNNNNNT GNNNANNTNN NGGGNNNCCN
          1021 NNCNNNNNCN NNNNNNNNN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Song, J., Two consecutive zinc fingers in Sp1 and in MAZ are essential for interactions with cis-elements. J. Biol. Chem., 276, 30429-30434 (2001). PMID 11395515.
          user report Wang H.Y., Transcriptional regulation of human eosinophil RNases by an evolutionary- conserved sequence motif in primate genome. BMC Mol. Biol., 8:89 (2007). PMID 17927842.

          2022.02.09

          GNP_filter3_RDBDEP_html_220112.pl