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pGEX-SP1-delta23

Expression vector of human SP1 in E.coli

Catalog number RDB05970
Resource name pGEX-SP1-delta23
Clone info. Expression vector of human SP1 in E.coli. This clone contains a DNA fragment corresponding to the 1,889nt..4,174nt of NM_003109.1 (where its CDS is 56nt..2,392nt) with the deletion of 2nd and 3rd zinc finger (corresponding 2,004nt to 2,181nt was replaced with GATC). At the 3' UTR, there are 36nt deletion corresponding to 2,408nt to 2,443nt and, 8nt deletion corresponding to 4,112nt to 4,119nt. There is not thrombin site on this clone.
Vector backbone pGEX-2TK (Plasmid)
Size of vector backbone 4.97 kb
Selectable markers Amp^r
Gene/insert name Human SP1 cDNA
Depositor|Developer Yokoyama, Kazunari | Song, Jun |
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External Database
Human SP1

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (J. Biol. Chem., 276, 30429-30434 (2001)).
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (J. Biol. Chem., 276, 30429-30434 (2001))。
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          RDB05970 pGEX-SP1-delta23 DNA solution

          Ordering Information [in Japanese] [in English]
          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Song, J., Two consecutive zinc fingers in Sp1 and in MAZ are essential for interactions with cis-elements. J. Biol. Chem., 276, 30429-30434 (2001). PMID 11395515.

          Further references such as user reports and related articles (go to bottom)

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          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB19170_B1F4p1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pGEX-F2
          Region: GST,insert 5'
          Sequence file: RDB19170_B1F4a.seq check
          >05970_19170_B1F4_3_pGEX-F2_C01_03_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNG TATTGNNGCT ATCCCNCAAA TTGATAAGTA CTTGAAATCC
             61 AGCAAGTATA TAGCATGGCC TTTGCAGGGC TGGCAAGCCA CGTTTGGTGG TGGCGACCAT
            121 CCTCCAAAAT CGGATCCTGG CAAAAAGAAA CAGCATATTT GCCACATCCA AGGCTGTGGG
            181 AAAGTGTATG GCAAGACCTC TCACCTGCGG GCACACTTGC GCTGGCATAC AGGCGAGAGG
            241 CCATTTAGAT CTGTAGCTCT GAGTGTGGGC ACTTTGCCCC TGGACAGTGG GGCAGGTTCA
            301 GAAGGCAGTG GCACTGCCAC TCCTTCAGCC CTTATTACCA CCAATATGGT AGCCATGGAG
            361 GCCATCTGTC CAGAGGGCAT TGCCCGTCTT GCCAACAGTG GCATCAACGT CATGCAGGTG
            421 GCAGATCTGC AGTCCATTAA TATCAGTGGC AATGGCTTCT GAGATCAGGC ACCCGGGATG
            481 CAAGGTAGCA TGGGTCCAAG AGACATGGAA GAGAGAGCCA TGAAGCATTA AAATGCATGG
            541 TGTTGAGAAG AATCAGGAGA GGGATACAAG AGAGGAGANG GGGTCCCGGC ACCCATCTGT
            601 ATCATCAGTG CCTCTTTGAA NGTGGGAAAC ATTAGTGNAA ATTCTGNTGG TGCCACGCTT
            661 TGNNGANCAN TTGTTTGACC CNNGTTTCNN CNNACNNNTC NNANCCNANN NNNNCCNTNN
          721 NNNNNNTNNN NNNNNNN
          //
          Primer: pGEX-RSEQ
          Region: insert 3'
          Sequence file: RDB19170_B1F4b.seq check
          >05970_19170_B1F4_3_pGX-RSEQ_D01_04_ABI08.ab1
              1 NNNNNNNNNN NNNNNGNNGC ANANCGTCNG TCAGTCACGA TGAATTCCGG TGCAGGTCTC
             61 TTTTAATATT GGCTAGCAGA AAACCTATCC CCAACCTAAC AGATTACACA CACACACACA
            121 CACACACACA CACAGAGCTA TTTTTCCACA CTTCCTCACA GTGAAAAGCT CTCTAACAAG
            181 ATACCAGAAG GTCGAGAGAG ACAATGCTTT TATGGCTGGG CCTTGAGGAC AATTCTGGTT
            241 GCATTTAAAT CTCAGTGCCA AATGCACTAG GTTCCCTGCC GTTATAATTT TTGGATCAGT
            301 CTCTTCTGAA ATCAGGTCAC AGAGAAAACA GGATGATTTC TCCCAATCTC TCCTATAAAA
            361 CACTGTGGTA AGACAAGATT TCTTTTTGTT CTGGGCTATC ATTCCCATGA TCATGTGCTT
            421 CCCTTTCCCT TAGGAACCAC CCAAGAACAT ACAAGGAAAA GTTAAGGGAA AACATCATAA
            481 GAACCACTGT CTTGCCACTA GCACATGCTT TGGAGGAGTT CTGCAGAATG GAAACCTGAA
            541 ACAGATTCAC TGGCTGANGC TCCTTATTGA CAATGGAAAA GAACCTAGTG GGGANGTTCA
            601 AAAGAAGTGN ATTTCCTTTC NNANTATAAA CTCANAAGCC CACNCNTCAA AACTTANANN
            661 TACCNNNNAA NANAGCANNN NNNNNTNNNN NACNCNNTNN CTNNNGNNNN NGANTNNNNN
          721 NNNNTTNNNN NNNNNN
          //
          Primer: lacIpro-B_F
          Region: LacI pro,lacI
          Sequence file: RDB19170_B1F4c.seq check
          >05970_19170_B1F4_3_lacIpro-B_F_E01_05_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNG NGTCNGTGAG CGAGGAAGCG GAAGAGCGCC TGATGCGGTA
             61 TTTTCTCCTT ACGCATCTGT GCGGTATTTC ACACCGCATA AATTCCGACA CCATCGAATG
            121 GCGCAAAACC TTTCGCGGTA TGGCATGATA GCGCCCGGAA GAGAGTCAAT TCAGGGTGGT
            181 GAATGTGAAA CCAGTAACGT TATACGATGT CGCAGAGTAT GCCGGTGTCT CTTATCAGAC
            241 CGTTTCCCGC GTGGTGAACC AGGCCAGCCA CGTTTCTGCG AAAACGCGGG AAAAAGTGGA
            301 AGCGGCGATG GCGGAGCTGA ATTACATTCC CAACCGCGTG GCACAACAAC TGGCGGGCAA
            361 ACAGTCGTTG CTGATTGGCG TTGCCACCTC CAGTCTGGCC CTGCACGCGC CGTCGCAAAT
            421 TGTCGCGGCG ATTAAATCTC GCGCCGATCA ACTGGGTGCC AGCGTGGTGG TGTCGATGGT
            481 AGAACGAAGC GGCGTCGAAG CCTGTAAAGC GGCGGTGCAC AATCTTCTCG CGCAACGCGT
            541 CAGTGGGCTG ATCATTAACT ATCCGCTGGA TGANCAGGAT GCCNTTGCTG TGGAANCTGC
            601 CTGCACTAAT GNTCCNGCGT TATTTCNTGA NGTCNNTNAN NNNANNNNNT CNNNNGTANN
          661 ANTTTCNCNN NGNNNNNNNN ACNNNNNNNG NNNNNNNNNN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Song, J., Two consecutive zinc fingers in Sp1 and in MAZ are essential for interactions with cis-elements. J. Biol. Chem., 276, 30429-30434 (2001). PMID 11395515.

          2022.02.09

          GNP_filter3_RDBDEP_html_220112.pl