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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pGEX-SP1-delta23 (#RDB05970)

Expression vector of human SP1 in E.coli

Clone info. Expression vector of human SP1 in E.coli. This clone contains a DNA fragment corresponding to the 1,889nt..4,174nt of NM_003109.1 (where its CDS is 56nt..2,392nt) with the deletion of 2nd and 3rd zinc finger (corresponding 2,004nt to 2,181nt was replaced with GATC). At the 3' UTR, there are 36nt deletion corresponding to 2,408nt to 2,443nt and, 8nt deletion corresponding to 4,112nt to 4,119nt. There is not thrombin site on this clone.
Vector backbone pGEX-2TK (Plasmid)
Size of vector backbone 4.97 kb
Selectable markers Amp^r
Gene/insert name Human SP1 cDNA
Depositor|Developer Yokoyama, Kazunari | Song, Jun |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (J. Biol. Chem., 276, 30429-30434 (2001)).
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (J. Biol. Chem., 276, 30429-30434 (2001))。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB05970 pGEX-SP1-delta23 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pGEX-SP1-delta23 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB05970).

Reference section:

Song, J., Ugai, H., Ogawa, K., Wang, Y., Sarai, A., Obata, Y., Kanazawa, I., Sun, K., Itakura, K., Yokoyama, K.K., Two consecutive zinc fingers in Sp1 and in MAZ are essential for interactions with cis-elements. J. Biol. Chem., 276, 30429-30434 (2001). PMID 11395515. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB19170_B1F4p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pGEX-F2 (Pr0403)
Region: GST,insert 5'
Sequence file: RDB19170_B1F4a.seq check
>05970_19170_B1F4_3_pGEX-F2_C01_03_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNG TATTGNNGCT ATCCCNCAAA TTGATAAGTA CTTGAAATCC
   61 AGCAAGTATA TAGCATGGCC TTTGCAGGGC TGGCAAGCCA CGTTTGGTGG TGGCGACCAT
  121 CCTCCAAAAT CGGATCCTGG CAAAAAGAAA CAGCATATTT GCCACATCCA AGGCTGTGGG
  181 AAAGTGTATG GCAAGACCTC TCACCTGCGG GCACACTTGC GCTGGCATAC AGGCGAGAGG
  241 CCATTTAGAT CTGTAGCTCT GAGTGTGGGC ACTTTGCCCC TGGACAGTGG GGCAGGTTCA
  301 GAAGGCAGTG GCACTGCCAC TCCTTCAGCC CTTATTACCA CCAATATGGT AGCCATGGAG
  361 GCCATCTGTC CAGAGGGCAT TGCCCGTCTT GCCAACAGTG GCATCAACGT CATGCAGGTG
  421 GCAGATCTGC AGTCCATTAA TATCAGTGGC AATGGCTTCT GAGATCAGGC ACCCGGGATG
  481 CAAGGTAGCA TGGGTCCAAG AGACATGGAA GAGAGAGCCA TGAAGCATTA AAATGCATGG
  541 TGTTGAGAAG AATCAGGAGA GGGATACAAG AGAGGAGANG GGGTCCCGGC ACCCATCTGT
  601 ATCATCAGTG CCTCTTTGAA NGTGGGAAAC ATTAGTGNAA ATTCTGNTGG TGCCACGCTT
  661 TGNNGANCAN TTGTTTGACC CNNGTTTCNN CNNACNNNTC NNANCCNANN NNNNCCNTNN
721 NNNNNNTNNN NNNNNNN
//
Primer: pGEX-RSEQ (Pr0077)
Region: insert 3'
Sequence file: RDB19170_B1F4b.seq check
>05970_19170_B1F4_3_pGX-RSEQ_D01_04_ABI08.ab1
    1 NNNNNNNNNN NNNNNGNNGC ANANCGTCNG TCAGTCACGA TGAATTCCGG TGCAGGTCTC
   61 TTTTAATATT GGCTAGCAGA AAACCTATCC CCAACCTAAC AGATTACACA CACACACACA
  121 CACACACACA CACAGAGCTA TTTTTCCACA CTTCCTCACA GTGAAAAGCT CTCTAACAAG
  181 ATACCAGAAG GTCGAGAGAG ACAATGCTTT TATGGCTGGG CCTTGAGGAC AATTCTGGTT
  241 GCATTTAAAT CTCAGTGCCA AATGCACTAG GTTCCCTGCC GTTATAATTT TTGGATCAGT
  301 CTCTTCTGAA ATCAGGTCAC AGAGAAAACA GGATGATTTC TCCCAATCTC TCCTATAAAA
  361 CACTGTGGTA AGACAAGATT TCTTTTTGTT CTGGGCTATC ATTCCCATGA TCATGTGCTT
  421 CCCTTTCCCT TAGGAACCAC CCAAGAACAT ACAAGGAAAA GTTAAGGGAA AACATCATAA
  481 GAACCACTGT CTTGCCACTA GCACATGCTT TGGAGGAGTT CTGCAGAATG GAAACCTGAA
  541 ACAGATTCAC TGGCTGANGC TCCTTATTGA CAATGGAAAA GAACCTAGTG GGGANGTTCA
  601 AAAGAAGTGN ATTTCCTTTC NNANTATAAA CTCANAAGCC CACNCNTCAA AACTTANANN
  661 TACCNNNNAA NANAGCANNN NNNNNTNNNN NACNCNNTNN CTNNNGNNNN NGANTNNNNN
721 NNNNTTNNNN NNNNNN
//
Primer: lacIpro-B_F (Pr0724)
Region: LacI pro,lacI
Sequence file: RDB19170_B1F4c.seq check
>05970_19170_B1F4_3_lacIpro-B_F_E01_05_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNG NGTCNGTGAG CGAGGAAGCG GAAGAGCGCC TGATGCGGTA
   61 TTTTCTCCTT ACGCATCTGT GCGGTATTTC ACACCGCATA AATTCCGACA CCATCGAATG
  121 GCGCAAAACC TTTCGCGGTA TGGCATGATA GCGCCCGGAA GAGAGTCAAT TCAGGGTGGT
  181 GAATGTGAAA CCAGTAACGT TATACGATGT CGCAGAGTAT GCCGGTGTCT CTTATCAGAC
  241 CGTTTCCCGC GTGGTGAACC AGGCCAGCCA CGTTTCTGCG AAAACGCGGG AAAAAGTGGA
  301 AGCGGCGATG GCGGAGCTGA ATTACATTCC CAACCGCGTG GCACAACAAC TGGCGGGCAA
  361 ACAGTCGTTG CTGATTGGCG TTGCCACCTC CAGTCTGGCC CTGCACGCGC CGTCGCAAAT
  421 TGTCGCGGCG ATTAAATCTC GCGCCGATCA ACTGGGTGCC AGCGTGGTGG TGTCGATGGT
  481 AGAACGAAGC GGCGTCGAAG CCTGTAAAGC GGCGGTGCAC AATCTTCTCG CGCAACGCGT
  541 CAGTGGGCTG ATCATTAACT ATCCGCTGGA TGANCAGGAT GCCNTTGCTG TGGAANCTGC
  601 CTGCACTAAT GNTCCNGCGT TATTTCNTGA NGTCNNTNAN NNNANNNNNT CNNNNGTANN
661 ANTTTCNCNN NGNNNNNNNN ACNNNNNNNG NNNNNNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Song, J., Two consecutive zinc fingers in Sp1 and in MAZ are essential for interactions with cis-elements. J. Biol. Chem., 276, 30429-30434 (2001). PMID 11395515. [link to RRC of NBRP]