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pKM2L-phBMHC (#RDB05901)

Promoter Bank clone, Human cardiac beta-myosin heavy chain (beta-MHC) promoter

Alternative name p1987-6F
Clone info. PCR amplified human cardiac beta-myosin heavy chain (beta-MHC) promoter sequence was inserted into a Renilla luciferase reporter vector pKM2L (RDB04026). Cloned fragment: 23437311 to 23435620(NC_000014.9); relatively -1642 to +50, where +1 corresponds to 1 nt of NM_000257.4.
Comment PCR cloning, forward primer, 1987 F2: 5' agactactgggataacctcagcaac 3'; reverse primer, 1987 R2: 5' ctgagagcagcagggaaagaca 3' (reference: J. Biol. Chem., 267, 9917-9924, 1992, PMID:1577822) Entire sequence of promoter region has not been confirmed.
Vector backbone pKM2L (RDB04026) (Plasmid)
Size of vector backbone 4.2 kb
Selectable markers Kan^r
Gene/insert name Human cardiac beta-myosin heavy chain (beta-MHC) genomic DNA
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The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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RDB05901 pKM2L-phBMHC DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pKM2L-phBMHC was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB05901).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15712_A7L7p1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: M13_-40
Sequence file: RDB15712_A7L7a.seq check
>05901_15712_A7L7_M13_-40_G01_19_ABI24.ab1
    1 CAGCCAGGCC AGCTTGCATG CCTGCAGGTC ATTTAAATAG GGATAACAGG GTAATGATTA
   61 AGATCTCGAG CTCTAAGCTT CACATATGCA TGCACTAGTG GCAGACTACT GGGATAACCT
  121 CAGCAACTGT ATACCTTAGC ACTGCAGAGC TGCGGTGTTG TCAGACACAG CCCTTGCAGT
  181 TCGTGGTCTA CCCCCACTTG CCTGCACCCC CCTTATAGCT GCTCAATGTC CTCAGACTGT
  241 ACCTTAGTGC CTTGGTTACC AAGCAACCCC CACAGAGGCC TCCTCCTGGA TTTTCCCTTC
  301 CCTGCTATGC CCACCCCACC TTCAAAATGT ACCTGCTGCA CCCATCCCCC AGGCCTGCCT
  361 CACTCCTTCC AAGCTGACAG CTAATCAGAT GGACTAATCC GCATGCGCGG AAGAGCTGTG
  421 CTTGGGAAGA AGGGGGAGAT TTCACACCAA ATCGTGCAGT GGTTGCCTAG ACACGGAGCC
  481 AGTCAAGCTT GCATGCCCCG TTGCTAGAAG AGCATGGCAT CGGTTGTGTT CTGTGCTCGT
  541 AGGCTGGGCC CCAGCTCCCT TTCCTCCCCT CCTTTGTGGC TGTTCCTCAG GCCTGCAGAG
  601 GGGAAGATTT TTCCTGTCCT GTTACTCTGC CTCACTTGCC AGAACACTAG TCGCCTCCAG
  661 CTCTGGATAA AGCTGAGGCT GGGTGGGCCA GACAACAGCT GTCTTAGGGG TCTCCTTCCA
  721 GCTCCAAGTA GTTGTATTAT CTATAGCTCT GAGAGGGTCT GTCTCTGGGC TCAGGCCTTT
  781 AGTACCTGTG GGGCAGAGCC AGGAACGTGA GTCAGGCAAA CCGCTCCAAA CTAGGACCAA
  841 TGCAGCTGGG TCCTGCTCTC TGCGCATAGG GGGTCCCCAC ATCTTTGGAA TCCCAGCCCA
  901 CCTTTCCAGG CTACCCTCCA CAGGCCGGGC TCCACTCCCA TCTGCTGAGG TTTCCCACCC
  961 TTGAAATCCC TGCTGCCCCG ATTTAGCTGT ATAACCCTTT AAAGAATGCA CTGTCCCTCT
 1021 CATTTAAAAT GAAGTGCTTG GATGGATGCT TAAAGGACCC TGGATCTGGC TCGAAGGCCT
1081 GGATGACGTC AACAACATTT GACCTTG
//
Primer: phRLR2
Sequence file: RDB15712_A7L7b.seq check
>05901_15712_A7L7_phRLR2_H01_22_ABI24.ab1
    1 ATCTTTGTCT GACAAGCCCA GTTTCTATTG GTCTCCTTAA ACCTGTCTTG TAACCTTGAT
   61 ACTTACCTGC CCAGTGCCTC ACGACCAACT TCTGCAGTAC CCGGGTGAAT TCTACGCGTC
  121 GACAGGCCTG AGAGCAGCAG GGAAAGACAC AGAGCAGGAC AGAGCTGTGG AGCCAGACCT
  181 GGTCTCAGGG GCTGTATATA TGGGGCAGCA GGGCACCCCC ACCCCCAACG GCACCCAGTC
  241 CCTAGCCGGA TTTAGAAAGG GCTGTTGTCA CTAGAAGCAT TTCCCCCAGG CTCCCCCCCT
  301 TCCCAAACAG TGCTCCCCTC CCCGCCTCTC CAGCTAGGAA ACAATTGGAA GTGGTCGTCA
  361 TTGTTATGGC ATGGACTGTG CAGGGTCTGA GTTCCAGGGG GCGGGGTGGC TCAAAGTGCA
  421 GCAAAAATAT CTCCCAGGCC AGGCCTCACA TTCCACAGCT GGCAGGAGAG GGAACTGACC
  481 ACGACCACCA AGACCACAGG CAGCCAATGT CCTGGCTCTC CCGCAGGGCA CTTCTGGCCC
  541 TGGACACTGC TCCCCCTCCA GTCCCCTGCT GCGAGGCCCC TGATGGCCTG GCCGCATTTC
  601 CACAGTGTCC TCCCTGCTCT CCTGGCCCAG GTCCTGTATC CAACTGGAAG CTGGGAAGGG
  661 GCTGAGTGTC AGCAGCAGAG AAGGGTAGGA CAGAGTTGGG GGCATGGCCC CAGGGTGAGG
  721 CAGCTAGACT GGGGCAGCTG GAAGGACATT TCCTAGCCAG GTGGCAAGAA CAATAACCAT
  781 GGAGCTTAGA GATGGGGACT CTGGCTCAGG CACTGATTTC CTTGGACCAG CAGGCAATGT
  841 GTTGAGGTCA CCAAGCCTCC GAGCCAGACA GGCCTTTAGC AATCCATCCA AGCACTTCAT
  901 TTAATGGGAG AGGGACAGTG CATTCTTTAA AGTTATACAG CTAATCGGGG CAGCAGGATT
  961 CAAGGTGGAA ACCTCAGCAG ATGGGAGTGG AGCCCGGGCC TGTGAGGTAG CCTGAAAGGT
 1021 TGGGCTGGGA TTCCAAAGAT GTGGGACCCC CTATGCGCAA GAGCAGACCA GCTGCATGGT
1081 TCTTAGTTGG AAGCGGAATT GACGTGA
//
Primer: pAxCALNL_F1
Sequence file: RDB15712_A7L7c.seq check
>05901_15712_A7L7_pAxCALNL_F1_A02_02_ABI24.ab1
    1 TCAAGCATCT TATCATGTCT GCTCGAGCGG CCGCTCTAGA ATTACTGCTC GTTCTTCAGC
   61 ACGCGCTCCA CGAAGCTCTT GATGTACTTA CCCATTTCAT CTGGAGCGTC CTCCTGGCTG
  121 AAGTGGAGGC CCTTCACCTT CACGAACTCG GTGTTAGGGA ACTTCTTAGC TCCCTCGACA
  181 ATAGCGTTGG AAAAGAACCC AGGGTCGGAC TCGATGAACA TCTTAGGCAG ATCGTCGCTG
  241 GCCCGAAGGT AGGCGTTGTA GTTGCGGACA ATCTGGACGA CGTCGGGCTT GCCTCCCTTA
  301 ACGAGAGGGA TCTCGCGAGG CCAGGAGAGG GTAGGCCGTC TAACCTCGCC CTTCTCCTTG
  361 AATGGCTCCA GGTAGGCAGC GAACTCCTCA GGCTCCAGTT TCCGCATGAT CTTGCTTGGG
  421 AGCATGGTCT CGACGAAGAA GTTATTCTCA AGCACCATTT TCTCGCCCTC TTCGCTCTTG
  481 ATCAGGGCGA TATCCTCCTC GATGTCAGGC CACTCGTCCC AGGACTCGAT CACGTCCACG
  541 ACACTCTCAG CATGGACGAT GGCCTTGATC TTGTCTTGGT GCTCGTAGGA GTAGTGAAAG
  601 GCCAGACAAG CCCCCCAGTC GTGGCCCACA AAGATGATTT TCTTTGGAAG GTTCAGCAGC
  661 TCGAACCAAG CGGTGAGGTA CTTGTAGTGA TCCAGGAGGC GATATGAGCC ATTCCCGCTC
  721 TTGCCGGACT TACCCATTCC GATCAGATCA GGGATGATGC ATCTAGCCAC GGGCTCGATG
  781 TGAGGCACGA CGTGCCTCCA CAGGTAGCTG GAGGCAGCGT TACCATGCAG AAAAATCACG
  841 GCGTTCTCGG CGTGCTTCTC GGAATCATAG TAGTTGATGA AGGAGTCCAG CACGTTCATT
  901 TGCTTGCAGC GAGCCCACCA CTGAGGCCCA GTGATCATGC GTTTGCGTTG CTCGGGGTCG
  961 TACATCTTTG GAAGCCATGG TGGCTAGCCT ATAGTGAGTC GTATTTAAGT ACTCTAGGCC
 1021 TTTAGAGCCT GTAATTGACC TGGGGAAGTG ACACTGTGAG AAGACGGCAA GTGTATGTCA
1081 GTTAAGACTA TAGTTTACCC A
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles