RDB05892 - pKM2L-phLCK

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pKM2L-phLCK (#RDB05892)

Promoter Bank clone, Human lck protein tyrosine kinase (upstream promoter) promoter

Alternative name	p1977-2F
Clone info.	PCR amplified human lck protein tyrosine kinase (upstream promoter) promoter sequence was inserted into a Renilla luciferase reporter vector pKM2L (RDB04026) (reference: Mol. Cell. Biol., 9, 2173-2180, 1989, PMID:2787474). Cloned fragment: 32248441 to 32251340(NC_000001.11); relatively -25670 to -22770, where +1 corresponds to 1 nt of NM_001042771.2; relatively -2824 to +76, where +1 corresponds to 1 nt of XM_024447046.1.
Comment	PCR cloning, forward primer, 1977 F2: 5' tgaagatggaccaagcctgtcacct 3'; reverse primer, 1977 R2: 5' cacatggtcctcctggcctaacctg 3'; Entire sequence of promoter region has not been confirmed. Promoter activity has not been analyzed.
Vector backbone	pKM2L (RDB04026) (Plasmid)
Size of vector backbone	4.2 kb
Selectable markers	Kan^r
Gene/insert name	Human lck protein tyrosine kinase (upstream promoter) genomic DNA
Depositor\|Developer	DNA Bank, \|

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The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.

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Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	1. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. 2. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. Additional terms and conditions: The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.

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MTAに書く使用条件	1. 本件リソースは学術研究機関にのみ提供し、学術研究にのみ利用することができる。　2. 謝辞の表明を必要とする。 MTAに書く付加的使用条件：本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB05892	pKM2L-phLCK	DNA solution	JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pKM2L-phLCK was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB05892).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB05892_A4Kr.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: M13_-40 Sequence file: RDB05892_A4Kra.seq
>05892_05892_A4Kr_1_M13_-40_A06_03.ab1 1 TAGGCGCGCC GGCCGGTTCC AAGCCTTGCA TGCCTGGCAG GTCATTTAAA ATAGGGATAA 61 CAGGGTAAAT GATTAAGGAT CTCGAGCTCT AAGCTTCACA TATGCATGCA CTAGTGGCTG 121 AAGATGGACC AAGCCTGTCA CCTGCACCAG GAGCTGCTGG ATGAGGCCGA GCTGGAGCTG 181 GAGCTGGAGC CCGGGGCCGG CCTAGCCCTG GCCCCGCTGC TGCGGCACCT GGGCCTCACG 241 CGCATGAACA TCAGCGCCCG GCGCTTCACC CTCTGCTGAG GAACACCTGT GCCCCCCGAC 301 TCCCCGCCCC CTCTCCCAAT GCCGCTTCCC CTGCCTGCCT GGGAAGAGGA AAGGGAGGGG 361 TGCCCCAGAG GCACCAGCTC CTGGCGGGGG AGGAGGAACA TTCAGGTTTC TGAGAGCTGA 421 ATTCCAAGAG TGCAAAACCC CAGCATCCTG TTTCCTCTGC TGACCCAGCT GGGAGGGGGA 481 GGAGGAGGAG CTCACACCCT CAAACTCCTC AATAAACGCT TTCTCTGGTT CCCATCAAGG 541 TCTCTTGCAG TCGTTTATTC AGGCCCAAGG CCGCTATCTG AATGTCGCCT GTCACTGGGA 601 CCACCCTAGT TCACTGCAGG CCCATGGGAG GTGACTTAGG TAAATTGGGC TCCTGAAGAA 661 TGCAGAATCT GAGAGACATG GGTTCAAAGC CAGCTACCCC GTTTATTTCT TTTTGTTGTT 721 GTTATTTCTT CTGCTTCTTT TTTTTTTTTT TTTTTGAGGC AGAGTCTCAC TCTGTCACCC 781 AGGCTGGAAT GCAGTAGCAC AATCTCAGCT CACTGCAACC TCCGCCTCCG GGGATCAAGC 841 GATTCTCCTG CCTCAGCCTC CCAAGGTAGC TGGGGATTAC AGGCGGCCAC CACCTCGCCT 901 GGGCTAATTT CCCTTTCTTT TCTTTTCTTT TTTTTTTTTC TTTTTTTTTG AGATGGAATT 961 TTCACTCTTT TCGCCCAGGC TGGGAGTAGT GGCGCGATCT TTGGGCTCAC TGCAACCTCC 1021 ACCTTTCTGA ATTCAAGCGA TTCCTCCCTG CCTCAGCCCT CCCAAGTAGC CTGGGAATTA 1081 CAGGGGGCCA CAACCTCGCC CTAGCTAATT TCCTTTCCTT TGCTTGTCCT TTTTATCTAT 1141 TGCTATTTTT TTTTGAGAAT GGAAATTTCA CCTCCATTTT TCCGCCCCCA GGGCTGTGGA 1201 AAAATAAGTG TGGGGGCGCA GCGAATATTC TGTTGTGGCG TCT //
Primer: phRLR2 Sequence file: RDB05892_A4Krb.seq
>05892_05892_A4Kr_1_phRLR2_D06_12.ab1 1 CGGGGGGGGG ATGCCGGCGA GCTCCGTTGC AGCCCAGTTT CTATTGGTCT CCTTAAACCT 61 GTCTTGTAAC CTTGATACTT ACCTGCCCAG TGCCTCACGA CCAACTTCTG CAGTACCCGG 121 GTGAATTCTA CGCGTCGACA GGCCACATGG TCCTCCTGGC CTAACCTGGC TGCCTGGACA 181 GTCTCAGCTC CCTTCGCCGT CGTAGCCACC ACCTGTCTCC CCGCGCCCAC GTCCCACCCT 241 GACTTCCTGC CTCCTCCGGA GGGCTCCGCA GCCAACCGGG CCCCAGCAGC CCAACCACAA 301 CCCTCTAGCA CGCCAAGCTT CCTGCCACCA TAGACCCCTG CCTCTCAGCC CCTGGGGGTC 361 CCCTACATCT CCAGACCAGG TCCATCTTCC CCTACTGTCC CAGGGTGACA CTGGGAGGTT 421 TAAAGTTGAG GGGAGGATGT CTGATAGACC CTCCCTTCTG CCCTCCAGGG TGCATCTGGG 481 CAAGAGATTC AAGGGCAGCC TTTGGGCCCA GTGAGCAGGG GGCAAGCGGA CACACACTCA 541 GCTTATGGTG AGAGGCAAAC AGCACCTCAG CACCACCGTT GCAGCTGAGA GACCCTTTTG 601 TTTCTGTCTG GCAGCAAAGG TAGCATCGTT CAGAGTCAGA GGAGAGGACA ATATCCCTGT 661 TAAATAGCTG GTGAGCAGAC CATGCATGTT ACAGTCAGGT CAGGCCGAGA GAACAATTCC 721 CATGTCATCT CAGGCATGGA ACAGTAGCTT TATTATAGTC AAGGGTAGGT GAATGGCAGG 781 CTACCATCCC TCTTACAGAC AGAATGCTGG AAACAGCCTG TTTCAGGAGG CAGACAGGAC 841 ACCTCCATGT CTTCAGAGGG CATAGTAACG GCCCCTGCTG TAGTCAGAGG CCAGACAACA 901 CCCATTATCA TGTTGGAAGA GTAGGCAACA GTTCGAATTC AAGTAGAGCA AGGCCCCAGC 961 CCTTGCAGAG TGGAGGGCTT GCACTATCTC TGCACAGTGA AGACAGGCAA CAGCGCTCAG 1021 TCTCAAGTGC AGCCAAGACT GTCAGGACTT CTGTTTAAGC CGAGCAGAGA ATTGTCCAGA 1081 GCACAGCAGC CAGATTCATT CTTGGCCAGC TAGGACCATT ATCTAGCAGT CAGATGGATT 1141 CAGGGCCTCT AAG //