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pKM2L-phMBP (#RDB05890)

Promoter Bank clone, Human myelin basic protein (MBP) promoter

Alternative name p1973-12F
Clone info. PCR amplified human myelin basic protein (MBP) promoter sequence was inserted into a Renilla luciferase reporter vector pKM2L (RDB04026). Cloned fragment: 77019999 to 77017024(NC_000018.10); relatively -2944 to +32, where +1 corresponds to 1 nt of NM_001025081.2.
Comment PCR cloning, forward primer, 1973 F2: 5' aggctgtgatacccctgctgttgct 3'; reverse primer, 1973 R2: 5' ggaggtggctgcactgtcttcttgg 3' Entire sequence of promoter region has not been confirmed. (reference: Brain Res. Dev. Brain Res., 65, 217-221, 1992, PMID:1373994; Gene, 313, 161-167, 2003, PMID:12957387; Gene, 75, 31-38, 1989, PMID:2470651)
Vector backbone pKM2L (RDB04026) (Plasmid)
Size of vector backbone 4.2 kb
Selectable markers Kan^r
Gene/insert name Human myelin basic protein (MBP) genomic DNA
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The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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RDB05890 pKM2L-phMBP DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pKM2L-phMBP was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB05890).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB13698_A5Jsp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: M13_-40
Sequence file: RDB13698_A5Jsa.seq check
>05890_13698_A5Js_M13(-40)_D11_11.ab1
    1 CCGGCAGCAG CTTGCATGCC TGCAGGTCAT TTAAATAGGG ATAACAGGGT AATGATTAAG
   61 ATCTCGAGCT CTAAGCTTCA CATATGCATG CACTAGTGGC AGGCTGTGAT ACCCCTGCTG
  121 TTGCTCACAG ATGGTCAGTT CCCTGCACAG GCCCTCCTGC CTCCCAGGTT ACTGCCTGGC
  181 CTGGGAGGTT CTCAGCCCGG CCATCCTCCT GGACCCAGAA CCAGCATGGC CGCTCTCCTG
  241 CTGCTTCCGC CACCGGGAGC CGATTCCGCG CAGGCTTCCA GTGGGGGCTG CCTTCCCCTC
  301 TGCCAGAAGC ACCCTCCCCA ACTTCCCCAA GTCCTCGCAG GGCCCAGACT CTCATCTTGT
  361 CCTGTCATTT CCGCAAGGAC CTTCCTGCTG ACCCAAGCCC TCTCTGCTTT CCTATTCTCT
  421 TGCCTGATGT GAAGTTGCAC ATTGCATGAG TTGGGGCTGC CAAGACAAAT TACCATATGC
  481 TGGGGGCTTC AAACTACAGG AATTCTTCTG TGACCATTGT GAAGGCCAGA AAGCTCAAGT
  541 TGAGATGTCT GCAGAGCCCT GCTTCCCCCG AAGGCTCTAC AGGAGAATTT GCTCCATGTC
  601 TCTTACTCCT GGTGGTGCTC AGAAGTGCTT GGTGTTCTGA GGTTTGTAGA TGCCCCCCTC
  661 CAGGCTCCTC CTCCATCTTC ACACCTCTCC CCTGTGCATC TGTGGCTGTG TCCAAACTGC
  721 TCTCTTCTTA CAAGGACACC AGTGATTGGA GTAGGGCCCA CCCTACTGCA GAATGACCTC
  781 ATCTTCACTT GATTCCACCT GCAAAGATCT ATTTCCCAAA TAAAGGTCAC ACTCACAGGT
  841 ACAGAAGTTA GGACATGGGA CATATCATTT GGAGGGATAC TATTTCAATC CCATAACATA
  901 CATTTTCACC TGCTTTCCCC CACTAGAATG TAGTTTCACG AGGACAAGAC CTCGTGAGTA
  961 CATCACTGTA CTCAGAATAT TTAGATGTAC ATTACATTAA TTTGTACTCA TAATTATTGG
1021 ATTGGATTGG ATTCATTGGT ATGTATGGTA TGTAACCAGG TACGCTTAAT TAGATTGG
//
Primer: phRLR2
Sequence file: RDB13698_A5Jsb.seq check
>05890_13698_A5Js_phRLR2_E11_14.ab1
    1 ACTCCCGGTC TCGAAGCCCA GTTTCTATTG GTCTCCTTAA ACCTGTCTTG TAACCTTGAT
   61 ACTTACCTGC CCAGTGCCTC ACGACCAACT TCTGCAGTAC CCGGGTGAAT TCTACGCGTC
  121 GACAGGCGGA GGTGGCTGCA CTGTCTTCTT GGATGGTCTG GAGCTCGTCG GACTCAGAGG
  181 GCCTGTCTTT GAAGGTGTTG TCCTCCCTCC CCGGGGCATC TCGGGAAAAG AGGCGGATCA
  241 AGTGGGGGCG GCTCCCTGGG TCAGCTGGGT GGGCATCCTG CCAGGCATTC TTCGGGTCCG
  301 CTGTGCGCTT GGAGTCAGTC ACCGCTGTGT CCTGAGAGGA GGTCCCATTG TTCTGGTTCG
  361 CATCTGCCTC TCCTGCAAAC AACAATGAGA TATGAATACG GGCCTGTGCA AAGCTGAGCA
  421 CCGGACAAAG CAGCTTTCTC TGAGGGGTCT TGACCTAGCT GTCTCCTATA AAACCCACAG
  481 AATATGTGCA GTCCTCATAA AGCCCTTGGC CTAGGATGGA GCTAACCTGG ATGGAGCAGC
  541 CTTATTGCAG CTCTGTGTTT CAGAAGGCTC TGGGAATTCC TCAGTTACTC GAGATTTGGC
  601 ACCCAGCGTA AAATGCACCC AGGCTTCCCG ATGAAGTCAT CTGAGCATGC AGAGCAGCCA
  661 CATCAGCGTT TCTTCCCGGG TGCTGCCATG GCTGCTCCCG GAGAGCCCAG ATGTGCAGGC
  721 ACCTTGATGG GACATTGACT GTTTAAAAGG CCACTGGTGC ATAAGTTAAA TATGCAAGGA
  781 GCATTTATTT GACAAAAGGT GTTTTTAGTT TTCTTGGTAG AAACAAAACT TGCCACATCT
  841 TTTTGCAATT CATGCATGAA AGAAGTGGGG GCATTTAGAA TTGTTTCAAT CCCAGATTCC
  901 ACAAGAGGCC TCTCACAATA GAGAACTGCT TATTCAGGAC ATTTTGACTT GGCTTCTCCT
  961 CTGGGAAAGC AGAAACAAAT TAGAAATCTG AAAGTGGGAT TGAGATATTT CATGTTTGTA
 1021 TACCCAGCAT CTCATAGCTT TTCCTCAAAA AAATACCACA TTTTCTTGTC ACGAAGGCCA
1081 ATTGCAACTC GATTTTGGCC TATCTAGTAC GAGTGATCAC GCATTTCAGA TCAT
//
Primer: pAxCALNL_F1
Sequence file: RDB13698_A5Jsc.seq check
>05890_13698_A5Js_pAxCALNL_F1_F11_17.ab1
    1 ACATTATCTT ATCATGTCTG CTCGAGCGGC CGCTCTAGAA TTACTGCTCG TTCTTCAGCA
   61 CGCGCTCCAC GAAGCTCTTG ATGTACTTAC CCATTTCATC TGGAGCGTCC TCCTGGCTGA
  121 AGTGGAGGCC CTTCACCTTC ACGAACTCGG TGTTAGGGAA CTTCTTAGCT CCCTCGACAA
  181 TAGCGTTGGA AAAGAACCCA GGGTCGGACT CGATGAACAT CTTAGGCAGA TCGTCGCTGG
  241 CCCGAAGGTA GGCGTTGTAG TTGCGGACAA TCTGGACGAC GTCGGGCTTG CCTCCCTTAA
  301 CGAGAGGGAT CTCGCGAGGC CAGGAGAGGG TAGGCCGTCT AACCTCGCCC TTCTCCTTGA
  361 ATGGCTCCAG GTAGGCAGCG AACTCCTCAG GCTCCAGTTT CCGCATGATC TTGCTTGGGA
  421 GCATGGTCTC GACGAAGAAG TTATTCTCAA GCACCATTTT CTCGCCCTCT TCGCTCTTGA
  481 TCAGGGCGAT ATCCTCCTCG ATGTCAGGCC ACTCGTCCCA GGACTCGATC ACGTCCACGA
  541 CACTCTCAGC ATGGACGATG GCCTTGATCT TGTCTTGGTG CTCGTAGGAG TAGTGAAAGG
  601 CCAGACAAGC CCCCCAGTCG TGGCCCACAA AGATGATTTT CTTTGGAAGG TTCAGCAGCT
  661 CGAACCAAGC GGTGAGGTAC TTGTAGTGAT CCAGGAGGCG ATATGAGCCA TTCCCGCTCT
  721 TGCCGGACTT ACCCATTCCG ATCAGATCAG GGATGATGCA TCTAGCCACG GGCTCGATGT
  781 GAGGCACGAC GTGCCTCCAC AGGTAGCTGG AGGCAGCGTT ACCATGCAGA AAAATCACGG
  841 CGTTCTCGGC GTGCTTCTCG GAATCATAGT AGTTGATGAA GGGAGTCCAG CACGTTTCAT
  901 TTGCTTGCAG CGAGCCTCAC TCACTGAGGC CCAGTGATCA TGCGTTTGCG TTTGCTCGGG
  961 GTCGTACACC TTTGGAAGCC CATGGGTGGC TAGCTATAGT GAGTCGTATT AAGTACTCTA
 1021 GCTTAGAGCT GTAATTGACC TGGGGAGTGG ACACCCTGTG GCAGAGAAAG CTAAGGTTGC
1081 AATGTTCAGG TAAGGACTCA T
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles