RDB05887 - pKM2L-phBMP7

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icon below as well as clone information before placing your order.

pKM2L-phBMP7 (#RDB05887)

Promoter Bank clone, Human bone morphogenetic protein 7 (BMP7) promoter

Alternative name	p1944-3F
Clone info.	PCR amplified human bone morphogenetic protein 7 (BMP7) promoter sequence was inserted into a Renilla luciferase reporter vector pKM2L (RDB04026). Cloned fragment: 57268601 to 57266779(NC_000020.11); relatively -1960 to -137, where +1 corresponds to 1 nt of NM_001719.3.
Comment	PCR cloning, forward primer, 1944F-SpeI: 5' gactactagtcctggaggcggctccttgatggag 3'; reverse primer, 1944R-SalI: 5' gactgtcgacctagagcgctcgacaaatgtttac 3'; Entire sequence of promoter region has not been confirmed. Promoter activity has not been analyzed. (reference: Bone, 29, 54-61, 2001, PMID:11472891)
Vector backbone	pKM2L (RDB04026) (Plasmid)
Size of vector backbone	4.2 kb
Selectable markers	Kan^r
Gene/insert name	Human bone morphogenetic protein 7 genomic DNA
Depositor\|Developer	DNA Bank, \|

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The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.

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Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	1. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. 2. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. Additional terms and conditions: The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.

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Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB05887	pKM2L-phBMP7	DNA solution	JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pKM2L-phBMP7 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB05887).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB13034_A4La.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: M13_-40 Sequence file: RDB13034_A4Laa.seq
>05887_13034_A4La_M13(-40)_D04_10.ab1 1 CGGCAGAGCC AAGCTTGCAT GCCTGCAGGT CATTTAAATA GGGATAACAG GGTAATGATT 61 AAGATCTCGA GCTCTAAGCT TCACATATGC ATGCACTAGT CCTGGAGGCG GCTCCTTGAT 121 GGAGTCTGCC ATGTGGGTTC GCTCATGGCC ATGTCTTCCT GCCCAGCATG GTGCTTGGCC 181 CTGGGACTGG CCACATAATA TCTGGGCCAG GTGCAAAATT AGTACGGGGC AGGGGGTACT 241 TTGTTCATAG GTGATTCAGA ACCACATATG GTGACCTCAG AGTAGGAAAC CAAGTGTGGG 301 GCCCTTAAGA GCTGGGGGGC CCTGTACGAC TGTCCAGGTT GCAGGCCCCA CAGCTCGCCT 361 CCTGATATCC TGTGCTCCAT GCTTGTCTGT TGAAGGAAGG AGTGAATGGA TGAAGAGCAG 421 GTGGTGGGGG TGGTTTGAGG GCCTTGCCTG GTGGGTGGGT AGAGGCCCCT CCCTGGCATG 481 GGGCTCAAGA CCTGTTCCAT CCCACAGCCT GGGGCCTGTG TGTAAATGGC CAGGACCTGC 541 AGGCTGGCAT TTTTCTGCTC CTTGCCTGGC CTCTGGCCTC CCCTTTCTCC ACCCATGTGG 601 CCCCTCAGGC TGCCATCTAG TCCAAAAGTC CCCAAGGGAG ACCCAGAGGG CCACTTGGCC 661 AAACTACTTC TGCTCCAGAA AACTGTAGAA GACCATAATT CTCTTCCCCA GCTCTCCTGC 721 TCCAGGAAGG ACAGCCCCAA AGTGAGGCTT AGCCAGAGCC CCTCCCAGAC AAGCGCCCCC 781 GCTTCCCCAA CCTCAGCCCT TCCCAGTTCA TCCCAAAGGC CCTCTGGGGA CCCACTCTCT 841 CACCCAGCCC CAGGAGGGGA AGGAGACAGG ATGAACTTTT ACCCCGCTGC CCTCACTGCC 901 ACTCTGGGTG CAGTAATTCC CTTGAGATCC CACACCGGCA GAGGGACCGG TGGGTTCTGA 961 GTGGTCTGGG GGACTCCCTG TGACAGCGTG CATGGCTCGG TATTGATTGA GGGATGAATG 1021 GATGAGGAGA GACAGGAGAG GAGGCCCGAT TGGGGAGTCT CAGCACAGAA CCCTTGCAGG 1081 GCAGAGAATG TGAAGACCAG CCGGCTGCTC CCCCAGGTCC AACTTGGCCC ACCGGAGG //
Primer: phRLR2 Sequence file: RDB13034_A4Lab.seq
>05887_13034_A4La_phRLR2_E04_13.ab1 1 CTTCTTTTCT GACAGCCCAG TTTCTATTGG TCTCCTTAAA CCTGTCTTGT AACCTTGATA 61 CTTACCTGCC CAGTGCCTCA CGACCAACTT CTGCAGTACC CGGGTGAATT CTACGCGTCG 121 ACCTAGAGCG CTCGACAAAT GTTTACTGAG TGCCCACGGG GTGCCTAGAG CCTGGCGGGA 181 CGTCGCGGGG ATGCGGCTGG AGGAATCGGA GCCGGCCCTG CCCTCTCGGC ACTCGCGGTC 241 CGGACCAGGA AGGCGGCCCG AGCAGGACGG GTGGGCTGCG GACCGCGGTG GGAAAAGTTG 301 GGGGAGCGCG GAGGTGGCGC GGAAATAGAA GGCTTCCTGG AAGCGGTGAC CCTTGAGCCG 361 GTCTTGCCCC TCAGTCCCTG TATCCTTTTA AGCCAGCCAC GCTGACCCCT CCGCGCCTCC 421 CTGAAGCCGT TGCCAGCCTG TCTCTGCGCT CGCCATTCCC TCCGCCTGGA CCTCTCCCCA 481 CTCACCACCT CCCGCCCCCA TCAGACGCTG CTTTCTCCTC CCAGAGACCT CCAAGACCTG 541 CCAAGCTGAG GTTGTTTCCT CGCCTCGCCC CTCGCACGTT TGTTTTCTTT CCTGTAGTTT 601 CTCTTGCTGT CTGGAATTGC TGTTTGAGTT ACTCCGTGCC TTCTTTATTG CCTTCTTACC 661 CCCGCTAGAC ACCCAGAGCA CTGGGGCCCG GATCAGCGAT CTTCTCCGCA CCTCTGGTAC 721 CCGCAGTGCC TTGAACCCAG CGCCTGGTAG CCACTGTAGC TGCGCAGTCA CGATTTGTTG 781 AGTGAATAAG TGAACTGCGT GTGAGCTGGG GGGGTTCTTC CCACCTCCTC CTCTTCGGGG 841 CCCTGTATCT TTCCAGCCAC CCCTCAGCCT GAGACCAGAC ACCCCAGCCC CACCACTAGG 901 GCTTCCCATC AGCCCTCCTC GTGGCAGTGC CTGGGGAGCC AGCCGCTGGT CTTCACATCC 961 TCTTCCCCTC CAAGGGTCTG TGCCTGAGAC CTCCCCATCG GCCTCCTCTC CTGTCTCTCC 1021 TCATCCATTC ATCCCTCAAT CAATACCGAG CCATGCCACG CCTGTCACAG GTAGTCCCAG 1081 ACACTCAGAC CAACCGGATC CCTCTGCCGG GTGTGTGGGA TTCTCTCAAA GGGG //