Please review the QC test results indicated by icon below as well as clone information before placing your order.

pKM2L-pvMMTV (#RDB05825)

Promoter Bank clone, MMTV promoter

Alternative name	p2009-59
Clone info.	The MMTV promoter sequence (PCR-amplified 1,320 bp) was inserted into a Renilla luciferase reporter vector pKM2L (RDB04026).
Comment	Forward primer, 2009F: 5' aagcttgggcagaaatggttgaac 3'; Reverse primer, 2009R: 5' tgccgcagtcggccgacctgaggg 3' Entire sequence of promoter region has not been confirmed.
Vector backbone	pKM2L (RDB04026) (Plasmid)
Selectable markers	Kan^r
Gene/insert name	MMTV MMTV genomic DNA
Depositor|Developer	DNA Bank, |

 

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Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link]

Terms and conditions for distribution

1. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. 2. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. Additional terms and conditions: The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.

提供案内 (日本国内) [open/close]

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB05825	pKM2L-pvMMTV	DNA solution	JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by icon below as well as clone information before placing your order.

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How to cite this biological resource

Materials & Methods section:

The pKM2L-pvMMTV was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB05825).

Reference section:

Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB05825_A4K4.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: M13_-40 Sequence file: RDB05825_A4K4a.seq
>05825_05825_A4K4_3_M13_-40_C07_07.ab1     1 CGGCAAGGCA GCTTGCATGC CTGCAGGTCA TTTAAATAGG GATAACAGGG TAATGATTAA    61 GATCTCGAGC TCTAAGCTTC ACATATGCAT GCACTAGTGG CAAGCTTGGG CAGAAATGGT   121 TGAACTCCCG AGAGTGTCCT ACACCTAGGG GAGAAGCAGC CAAGGGGTTG TTTCCCACCA   181 AGGACGACCC GTCTGCGCAC AAACGGATGA GCCCATCAGA CAAAGACATA TTCATTCTCT   241 GCTGCAAACT TGGCATAGCT CTGCTTTGCC TGGGGCTATT GGGGGAAGTT GCGGTTCGTG   301 CTCGCAGGGC TCTCACCCTT GACTCTTTTA ATAGCTCTTC TGTGCAAGAT TACAATCTAA   361 ACAATTCGGA GAACTCGACC TTCCTCCTGA GGCAAGGACC ACAGCCAACT TCCTCTTACA   421 AGCCGCATCG ATTTTGTCCT TCAGAAATAG AAATAAGAAT GCTTGCTAAA AATTATATTT   481 TTACCAATAA GACCAATCCA ATAGGTAGAT TATTAGTTAC TATGTTAAGA AATGAATCAT   541 TATCTTTTAG TACTATTTTT ACTCAAATTC AGAAGTTAGA AATGGGAATA GAAAATAGAA   601 AGAGACGCTC AACCTCAATT GAAGAACAGG TGCAAGGACT ATTGACCACA GGCCTAGAAG   661 TAAAAAAGGG AAAAAAGAGT GTTTTTGTCA AAATAGGAGA CAGGTGGTGG CAACCAGGGA   721 CTTATAGGGG ACCTTACATC TACAGACCAA CAGATGCCCC CTTACCATAT ACAGGAAGAT   781 ATGACTTAAA TTGGGATAGG TGGGTTACAG TCAATGGCTA TAAAGTGTTA TATAGATCCC   841 TCCCTTTTCG TGAAAGACTC GCCAGAGCTA GACCTCCTTG GTGTATGTTG TCTCAAGAAG   901 AAAAAGACGA CATGAAACAA CAGGTACATG ATTATATTTA TCTAGGAACA GGAATGCACT   961 TTTTGGGGAA AGATTTTCCA TACCAAGGAG GGGACAGTGG CTGGACTAAT AGAACATTAT  1021 TTCTGCAAAA ACTCATGGCA TGAGTTATTA TGATAGCCTT TATTTGACCC ATCTTGCGAT  1081 TCCAGGCTTT AAGTAAGGTT TTTTGATTAC CAAACCTGAT TCTAACGAGG CATGTGAGAC 1141 ATGATTCTTG ACCTTGGAAT TTGC //
Primer: phRLR2 Sequence file: RDB05825_A4K4b.seq
>05825_05825_A4K4_3_phRLR2_D07_10.ab1     1 ACCTTCTCCG TCTCGAAAGC CCAGTTTCTA TTGGTCTCCT TAAACCTGTC TTGTAACCTT    61 GATACTTACC TGCCCAGTGC CTCACGACCA ACTTCTGCAG TACCCGGGTG AATTCTACGC   121 GTCGACAGGC TGCCGCAGTC GGCCGACCTG AGGGTCGCCG GGGTCTGCGG GGGGACCCTC   181 TGGAAAGTGA AGGATAAGTG ACGAGCGGAG ACGGGATGGC GAACAGACAC AAACACACAA   241 GAGGTGAATG TTAGGACTGT TGCAAGTTTA CTCAAAAAAT CAGCACTCTT TTATATCTTG   301 GTTTACATAA GCATTTACAT AAGATTTGGA TAAATTCCAA AAGAACATAG GAAAATAGAA   361 CACTCAGAGC TCAGATCAGA ACCTTTGATA CCAAACCAAG TCAGGAAACC ACTTGTCTCA   421 CATCCTCGTT TTAAGAACAG TTTGTAACCA AAAACTTACT TAAGCCCTGG GAACCGCAAG   481 GTTGGGCCAA TAAAGGCTAT TCATAATAAC TCATGCCATG AGTTTTTGCA GAATAATGTT   541 CTATTAGTCC AGCCACTGTC CCCTCCTTGG TATGGAAAAT CTTTCCCCAA AAGTGCATTC   601 CTGTTCCTAG ATAAATATAA TCATGTACCT GTTGTTTCAT GTCGTCTTTT TCTTCTTGAG   661 ACAACATACA CCAAGGAGGT CTAGCTCTGG CGAGTCTTTC ACGAAAAGGG AGGGATCTAT   721 ATAACACTTT ATAGCCATTG ACTGTAACCC ACCTATCCCA ATTTAAGTCA TATCTTCCTG   781 TATATGGTAA GGGGGCATCT GTTGGTCTGT AGATGTAAAG GTCCCCTATA AGTCCCTGGT   841 TGCCACCACC TGTCTCCTAT TTTGACAAAA ACACTCTTTT TTCCCTTTTT TACTTCTAGG   901 CCTGTGGTCA TAGTCCTTGC ACCTGTTCTT CAATTGAGTT GAGCGTCTCT TTCTATTTTC   961 TATTCCCATT TCTAACTTCT GAATTTGAGT AAAAAATAGT ACTAAAAGGA TATGATCATT  1021 TCTTACATAG TAACTAATTA ATCTAACTAA TGATGTCTTA TTGGTAAAAT ATAATTTTAG  1081 CCAGCCATTC CTATTCATTT CTGAGACAAT TCGAATGCCG CCTTGTAAGA GGAAAGTTGT 1141 GG //

Please visit Sequencing and PCR primers for primer information.

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References

Original, user report and related articles

user_report	Oasa, S., Relationship Between Homodimeric Glucocorticoid Receptor and Transcriptional Regulation Assessed via an In Vitro Fluorescence Correlation Spectroscopy-Microwell System. Sci. Rep. 8 (1): 7488 (2018). PMID 29748590. [link to RRC of NBRP]

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External Database
MMTV MMTV

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Reference sequence
  M15122

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