Resource data sheet
Prev.
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pKM2L-phKIT

Promoter Bank clone, Human c-kit promoter

Catalog number RDB05812
Resource name pKM2L-phKIT
Alternative name p2007-39F
Clone info. PCR amplified human c-kit promoter sequence was inserted into a Renilla luciferase reporter vector pKM2L (RDB04026). Cloned fragment: 54657684 to 54658014(NC_000004.12); relatively -244 to +87, where +1 corresponds to 1 nt of NM_000222.2.
Comment PCR cloning, forward primer, 2007F2- SpeI: 5' gatcactagtaggggtggaaaggtggagagaga 3'; reverse primer, 2007R2- SalI: 5' gatcgtcgaccgcggtagctgcgatgggat 3' (reference: Jpn. J. Cancer Res., 84, 1136-1144, 1993, PMID:7506248)
Vector backbone pKM2L (RDB04026) (Plasmid)
Size of vector backbone 4.2 kb
Selectable markers Kan^r
Gene/insert name Human c-kit genomic DNA
Depositor|Developer DNA Bank, |
Other clones in our bank

External Database
Human c-kit

          Reference sequence
            AC006552

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR 1. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. 2. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
          Additional terms and conditions for distribution The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
          Ordering Please visit Information of Request for Distribution.[link] 
          Order form 
          [Credit Card Payment]  [Bank Transfer Payment]
          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          提供案内 (日本国内) [open/close]

          提供条件 1. 本件リソースは学術研究機関にのみ提供し、学術研究にのみ利用することができる。 2. 謝辞の表明を必要とする。
          付加的提供条件 本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB05812 pKM2L-phKIT DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Promoter Collection (Renilla Luciferase) (English text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB13911_A6Aep1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: M13_-40
          Sequence file: RDB13911_A6Aea.seq check
          >05812_13911_A6Ae_2_M13(-40)_B05_05.ab1
              1 AGGCAGTCAG CTTGCATGCC TGCAGGTCAT TTAAATAGGG ATAACAGGGT AATGATTAAG
             61 ATCTCGAGCT CTAAGCTTCA CATATGCATG CACTAGTAGG GGTGGAAAGG TGGAGAGAGA
            121 AAGGGGCTCC GGAGTCAAGA GCGGGGAGAG AGGGCGCGCG CGCCCTCCTC CTCCCGGCGG
            181 GCACAGCCCC CCGGCATTAA CACGTCGAAA GAGCAGGGGC CAGACGCCGC CGGGAAGAAG
            241 CGAGACCCGG GCGGGCGCGA GGGAGGGGAG GCGAGGAGGG GCGTGGCCGG CGCGCAGAGG
            301 GAGGGCGCTG GGAGGAGGGG CTGCTGCTCG CCGCTCGCGG CTCTGGGGGC TCGGCTTTGC
            361 CGCGCTCGCT GCACTTGGGC GAGAGCTGGA ACGTGGACCA GAGCTCGGAT CCCATCGCAG
            421 CTACCGCGGT CGACGCGTAG AATTCACCCG GGTACTGCAG AAGTTGGTCG TGAGGCACTG
            481 GGCAGGTAAG TATCAAGGTT ACAAGACAGG TTTAAGGAGA CCAATAGAAA CTGGGCTTGT
            541 CGAGACAGAG AAGACTCTTG CGTTTCTGAT AGGCACCTAT TGGTCTTACT GACATCCACT
            601 TTGCCTTTCT CTCCACAGGT GTCCACTCCC AGTTCAATTA CAGCTCTTAA GGCTAGAGTA
            661 CTTAATACGA CTCACTATAG GCTAGCCACC ATGGCTTCCA AGGTGTACGA CCCCGAGCAA
            721 CGCAAACGCA TGATCACTGG GCCTCAGTGG TGGGCTCGCT GCAAGCAAAT GAACGTGCTG
            781 GACTCCTTCA TCAACTACTA TGATTCCGAG AAGCACGCCG AGAACGCCGT GATTTTTCTG
            841 CATGGTAACG CTGCCTCCAG CTACCTGTGG AGGCACGTCG TGCCTCACAT CGAGCCCGTG
            901 GCTAGATGCA TCATCCCTGA TCTGATCGGA ATGGGTAAGT CCGGCAAGAG CGGGAAATGG
            961 CTCATATCGC CTCCTGGATC ACTACAAGTA CCCTCACCGC TTGGTTCGAG CTGCTGAACC
           1021 CTTCCCAATG AAAATCATCT TTTGTGGGCC ACGACTGGGG GGGGCTTTGT CTGGGCTTTT
           1081 CACTACTCTA CGAGCACAAG ACCAAGATCC AGTCAATCGG TCCATGCTTG GAGAAGGTGT
          1141 GTTCCGGTTG GAACCGGTGT GAC
          //
          Primer: pAxCALNL_F1
          Sequence file: RDB13911_A6Aeb.seq check
          >05812_13911_A6Ae_2_pAxCALNL_F1_E05_14.ab1
              1 ATAATGTATC TTATCATGTC TGCTCGAAGC GGCCGCTCTA GAATTACTGC TCGTTCTTCA
             61 GCACGCGCTC CACGAAGCTC TTGATGTACT TACCCATTTC ATCTGGAGCG TCCTCCTGGC
            121 TGAAGTGGAG GCCCTTCACC TTCACGAACT CGGTGTTAGG GAACTTCTTA GCTCCCTCGA
            181 CAATAGCGTT GGAAAAGAAC CCAGGGTCGG ACTCGATGAA CATCTTAGGC AGATCGTCGC
            241 TGGCCCGAAG GTAGGCGTTG TAGTTGCGGA CAATCTGGAC GACGTCGGGC TTGCCTCCCT
            301 TAACGAGAGG GATCTCGCGA GGCCAGGAGA GGGTAGGCCG TCTAACCTCG CCCTTCTCCT
            361 TGAATGGCTC CAGGTAGGCA GCGAACTCCT CAGGCTCCAG TTTCCGCATG ATCTTGCTTG
            421 GGAGCATGGT CTCGACGAAG AAGTTATTCT CAAGCACCAT TTTCTCGCCC TCTTCGCTCT
            481 TGATCAGGGC GATATCCTCC TCGATGTCAG GCCACTCGTC CCAGGACTCG ATCACGTCCA
            541 CGACACTCTC AGCATGGACG ATGGCCTTGA TCTTGTCTTG GTGCTCGTAG GAGTAGTGAA
            601 AGGCCAGACA AGCCCCCCAG TCGTGGCCCA CAAAGATGAT TTTCTTTGGA AGGTTCAGCA
            661 GCTCGAACCA AGCGGTGAGG TACTTGTAGT GATCCAGGAG GCGATATGAG CCATTCCCGC
            721 TCTTGCCGGA CTTACCCATT CCGATCAGAT CAGGGATGAT GCATCTAGCC ACGGGCTCGA
            781 TGTGAGGCAC GACGTGCCTC CACAGGTAGC TGGAGGCAGC GTTACCATGC AGAAAAATCA
            841 CGGCGTTCTC GGCGTGCTTC TCGGAATCAT AGTAGTTGAT GAAGGAGTCC AGCACGTTCA
            901 TTTGCTTGCA GCGAGCCCAC CACTGAGGCC CAGTGATCAT GCGTTTGCGT TGCTCGGGGT
            961 CGTACACCCT TGGAAAGCCA TGGTGGCTAG CCTATAGTGA GTCGTATTAA GTACTCTAGC
           1021 CCTTAAGAGC TGTAATTTGA AACTGGGGAG TGGACACCTG TGGAGGAGAA GGCAAAAGTG
           1081 AATGTCAGTT AGATATAGTG CTTATCAGAA CGCAGAGTCC TTCCTCTGTC TCGACAAAGC
          1141 CAGTTTTCTA ATTTGC
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          user_report Kim, K.L., Direct and differential effects of stem cell factor on the neovascularization activity of endothelial progenitor cells. Cardiovasc Res. 92 (1): 132-140 (2011). PMID 21636540.

          2022.05.18

          GNP_filter3_RDBDEP_html_220516.pl