Resource data sheet
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PKC theta II / pFLAG (#RDB05725)

Expression vector of mouse PKC theta II

Clone info. The stop codon removed mouse PKC theta II cDNA was subcloned into pFLAG-CMV-5c to express FLAG tag at C terminus of PKC theta II. Expression vector of mouse PKC theta II.
Vector backbone pFLAG-CMV-5c (Plasmid)
Size of vector backbone 4.7 kb
Selectable markers Amp^r
Gene/insert name Mouse PKC thetaII cDNA
Depositor|Developer Hosoyamada, Akiyoshi | Niino, Yuko |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (J. Biol. Chem., 276, 36711-36717 2001).
提供案内 (日本国内) [open/close]

MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(J. Biol. Chem., 276, 36711-36717 2001)。

Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB05725 PKC theta II / pFLAG Under QC test. Please contact us. DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

How to cite this biological resource

Materials & Methods section:

The PKC theta II / pFLAG was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB05725).

Reference section:

Niino, Y.S., Irie, T., Takaishi, M., Hosono, T., Huh, N., Tachikawa, T., Kuroki, T., PKCtheta II, a new isoform of protein kinase C specifically expressed in the seminiferous tubules of mouse testis. J. Biol. Chem., 276, 36711-36717 (2001). PMID 11470790.

Further references such as user reports and related articles (go to bottom)

QC test results

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

original Niino, Y.S., PKCtheta II, a new isoform of protein kinase C specifically expressed in the seminiferous tubules of mouse testis. J. Biol. Chem., 276, 36711-36717 (2001). PMID 11470790.