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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pGEX-hMAZ

Expression vector of human MAZ in E. coli

Catalog number RDB05701
Resource name pGEX-hMAZ
Clone info. Expression vector of human MAZ in E. coli.
Comment This clone contains a cDNA fragment corresponding to the 174 to 1607nt of NM_002383.4. Known differences (at least): 15nt (ggcggcagcggcagc) deletion at 1493 to 1507nt.
Vector backbone pGEX-4T-2 (Plasmid)
Size of vector backbone 4.97 kb
Selectable markers Amp^r
Gene/insert name Human MYC-associated zinc finger protein (purine-binding transcription factor) cDNA
Depositor|Developer Yokoyama, Kazunari | Song, Jun |
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External Database
Human MYC-associated zinc finger protein (purine-binding transcription factor)

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR Manuscripts for the publication shall be made through the discussion between Depositor and Recipient Investigator. Recipient investigator shall include Depositor/Developer as co-author in manuscript for the publication. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Biochem. Biophys. Res. Commun., 226, 801-809 (1996).) Recipient Investigator shall negotiate with Developer/Depositor when patent application and the commercialization for the products based upon Material, derivatives thereof their use is considered.
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          提供条件 使用にあたっては常に密な連絡をとり、発表等に関しても事前に合意を要する。共著とする。利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Biochem. Biophys. Res. Commun., 226, 801-809 (1996).)。本リソースを使用して商業利用する場合は、寄託者と改めて契約する。
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          RDB05701 pGEX-hMAZ DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Song, J., Two consecutive zinc fingers in Sp1 and in MAZ are essential for interactions with cis-elements. J. Biol. Chem., 276, 30429-30434 (2001). PMID 11395515.

          Further references such as user reports and related articles (go to bottom)

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          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB12834_A4Gp.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pGEX_F
          Sequence file: RDB12834_A4Gpa.seq check
          >05701_12834_A4Gp_pGEX_F_B01_04.ab1
              1 GGCATCATCA ATCGGATCTG GTTCCGCGTG GATCCCCAGG AATTCTGCAG TCGACGGTAC
             61 CGGGCCCCGC TGCGGCCGAG GCCATGTTCC CGGTGTTTCC TTGCACGCTG CTGGCCCCCC
            121 CCTTCCCCGT GCTGGGCCTG GACTCCCGGG GGGTGGGCGG CCTCATGAAC TCCTTCCCGC
            181 CACCTCAGGG TCACGCCCAG AACCCCCTGC AGGTCGGGGC TGAGCTCCAG TCCCGCTTCT
            241 TTGCCTCCCA GGGCTGCGCC CAGAGTCCAT TCCAGGCCGC GCCGGCGCCC CCGCCCACGC
            301 CCCAGGCCCC GGCGGCCGAG CCCCTCCAGG TGGACTTGCT CCCGGTGCTC GCCGCCGCCC
            361 AGGAGTCCGC CGCGGCTGCT GCGGCCGCTG CCGCCGCTGC TGCCGCCGTC GCTGCCGCGC
            421 CCCCGGCCCC TGCCGCCGCC TCTACGGTGG ACACAGCGGC CCTGAAGCAG CCTCCGGCGC
            481 CCCCTCCGCC ACCCCCGCCA GTGTCGGCGC CCGCGGCCGA GGCCGCGCCC CCCGCCTCCG
            541 CCGCCACTAT CGCCGCGGCG GCGGCCACCG CCGTCGTAGC CCCAACCTCG ACGGTCGCCG
            601 TGGCCCCGGT CGCGTCTGCC TTGGAGAAGA AGACAAAGAG CAAGGGGCCC TACATCTGCG
            661 CTCTGTGCGC CAAGGAGTTC AAGAACGGCT ACAATCTCCG GAGGCACGAA GCCATCCACA
            721 CGGGAGCCAA GGCCGGCCGG GTCCCCTCGG GTGCTATGAA GATGCCGACC ATGGTGCCCC
            781 TGAGCCTCCT GAGCGTGCCC CAGCTGAGCG GAGCCGGCGG GGGAGGGGGA GAGGCGGGTG
            841 CCGGCGGCGG CGCTGCCGCA GTGGCCGCCG GTGGCGTGGT GACCACGACC GCCTCGGGGA
            901 AGCGCATCCG GAAGAACCAT GCCTGCGAGA TGTGTGGCAA GGCCTTCCGC GACGTCTACC
            961 ACCTGAACCG ACACAAGCTG TCGCACTCGG ACGAGAAGCC CTACCAGTGC CCGGTGTGCC
           1021 CAGCAGCGCT TCAAGCGCAA AGGACCGCAA TGAGCTACCA CGTGCGCCTC ACATGAACGC
          1081 GCTATGGCAC AAGCCTCTAC CACTACTCCC CAACTG
          //
          Primer: pGEX_R
          Sequence file: RDB12834_A4Gpb.seq check
          >05701_12834_A4Gp_pGEX_R_B02_05.ab1
              1 TGGGGGGGCT GTCATCCCGA ACGCGCGAGG CAGATCGTCA GTCAGTCACG ACCGCGGCCG
             61 CTCGAGTCGA CCCGGGAATT CCCCAAAAAA ACATTTCTTC CTCCTTTCCT TCTGGAGGCT
            121 CCTTGGTTGG TAGGGAAATA GGAGTTGGTG GGAAAAGAGG GGGGAGAGGA GCTTGTACCA
            181 AGGGACTCTA CTCCATTCTC CCCTCTCCCC CGCAACCAAC TTGGAGCTCA CCAGGGTTGG
            241 GAGGGAAGTG GCTGAGAGCT CACAGGCACC CCCTCCGCCC CCGAGAGGGA GCCCACAGCT
            301 GTGGGAGGGG CTGCTACTGC TGCCGCTGCT GCCGCTGCCG CCGCCATTGG ACAAACCTCA
            361 CCAGTACCTT TGTTGCAGAG CTCACAGACA TGGTGAGGAC CCTGGCTGTG CACCTTCATG
            421 TGGTCCGAAA TATAAGCCGA GCTCAGCATC TTGCCACACA CGTGACATGG CACTTTCTCC
            481 TCGTGTCGTA CTGTGTGCGC CCGCAGCCGA TCCTTCGTGG CGAAAGCTGC CTCACATTTC
            541 TCACATTTGA AGGGCCGTTC TGTTGAGTGC ACTTGTCTGA CGTGACTGTT GAGGTGATCC
            601 GGCCGGGAGA AGCTCTTGCC ACAGTGGGAG CAGTTGTAGG GCTTGTGCAC AGCGCCGTCA
            661 TGTGAGCGCA CGTGGTAGCT CATGCGGTCC TTGCGCTTGA AGCGCTGCTG GCACACCGGG
            721 CACTGGTAGG GCTTCTCGTC CGAGTGCGAC AGCTTGTGTC GGTTCAGGTG GTAGACGTCG
            781 CGGAAGGCCT TGCCACACAT CTCGCAGGCA TGGTTCTTCC GGATGCGCTT CCCCGAGGCG
            841 GTCGTGGTCA CCACGCCCAC CGGCGGCCAC TGCCGGCAGC GCCGCCCGCC GGGCACCCGC
            901 CTCTCCCCCT CCCCCGCCGG CTCCGCTTCA GCTTGGGGCA CCGCTTCATG AAGGCTCAGG
            961 GGCACCATGG TCGGCATTCT TCATAGCACC CGAAGGGACC CGGCCCGGCC TTGGCTTCCC
           1021 GTGTGATGGC TTCGTGCCTC CGGAAGATTG TAACCCGTCC TTGAACTTCC TTTGGCGCAC
           1081 AGAATCGCAC ATGTAGGGCC CCCTTGCTCC TTTTGGTCTT CTATCTCCAT GGTATAACGT
           1141 CCAACCTGGG TCTACAGCGG AACCCGTCTG AGGTTTGGTG CCACTCACTC ACTGGTCCAG
          1201 GTGTAGCGTC ACGCTGCACG GCTCTCA
          //
          Primer: MAZ-M_R2
          Sequence file: RDB12834_A4Gpc.seq check
          >05701_12834_A4Gp_MAZ-M_R2_A01_01.ab1
              1 AGCGATATGT GAGCGCACGT GGTAGCTCAT GCGGTCCTTG CGCTTGAAGC GCTGCTGGCA
             61 CACCGGGCAC TGGTAGGGCT TCTCGTCCGA GTGCGACAGC TTGTGTCGGT TCAGGTGGTA
            121 GACGTCGCGG AAGGCCTTGC CACACATCTC GCAGGCATGG TTCTTCCGGA TGCGCTTCCC
            181 CGAGGCGGTC GTGGTCACCA CGCCACCGGC GGCCACTGCG GCAGCGCCGC CGCCGGCACC
            241 CGCCTCTCCC CCTCCCCCGC CGGCTCCGCT CAGCTGGGGC ACGCTCAGGA GGCTCAGGGG
            301 CACCATGGTC GGCATCTTCA TAGCACCCGA GGGGACCCGG CCGGCCTTGG CTCCCGTGTG
            361 GATGGCTTCG TGCCTCCGGA GATTGTAGCC GTTCTTGAAC TCCTTGGCGC ACAGAGCGCA
            421 GATGTAGGGC CCCTTGCTCT TTGTCTTCTT CTCCAAGGCA GACGCGACCG GGGCCACGGC
            481 GACCGTCGAG GTTGGGGCTA CGACGGCGGT GGCCGCCGCC GCGGCGATAG TGGCGGCGGA
            541 GGCGGGGGGC GCGGCCTCGG CCGCGGGCGC CGACACTGGC GGGGGTGGCG GAGGGGGCGC
            601 CGGAGGCTGC TTCAGGGCCG CTGTGTCCAC CGTAGAGGCG GCGGCAGGGG CCGGGGGCGC
            661 GGCAGCGACG GCGGCAGCAG CGGCGGCAGC GGCCGCAGCA GCCGCGGCGG ACTCCTGGGC
            721 GGCGGCGAGC ACCGGGAGCA AGTCCACCTG GAGGGGCTCG GCCGCCGGGG CCTGGGGCGT
            781 GGGCGGGGGC GCCGGCGCGG CCTGGAATGG ACTCTGGGCG CAGCCCTGGG AGGCAAAGAA
            841 GCGGGACTGG AGCTCAGCCC CGACCTGCAG GGGGTTCTGG GCGTGACCCT GAGGTGGCGG
            901 GAAGGAGTTC ATGAGGCCGC CCACCCCCCC GGGAGTCCAG GCCCAGCACG GGGAAAGGGG
            961 GGGGCCAGCA GCGTGCAAGG AAACACCGGG AACATGGGCC TCGGCCGCAG CGGGGCCCGG
           1021 GTACCGTCGA CTGCAGATTC CCTGGGGGAT CCACGCGGGA CCAGAATCGG AATTTTGGAA
           1081 GAATGGATCG GCCACAGAAA CGTTGCCTGG CAGGCCTTGC AAGGTCATGC TAATAATACT
          1141 G
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Song, J., Two consecutive zinc fingers in Sp1 and in MAZ are essential for interactions with cis-elements. J. Biol. Chem., 276, 30429-30434 (2001). PMID 11395515.
          user_report Cogoi, S., MAZ-binding G4-decoy with locked nucleic acid and twisted intercalating nucleic acid modifications suppresses KRAS in pancreatic cancer cells and delays tumor growth in mice. Nucleic Acids Res. 2013 Apr;41(7):4049-64. PMID 23471001.
          user_report Membrino, A., G4-DNA Formation in the HRAS Promoter and Rational Design of Decoy Oligonucleotides for Cancer Therapy. PLoS One. 6 (9): e24421 (2011). PMID 21931711.
          user_report Cogoi S., The KRAS promoter responds to Myc-associated zinc finger and poly(ADP-ribose) polymerase 1 proteins, which recognize a critical quadruplex-forming GA-element. J. Biol. Chem., 285, 22003-22016 (2010). PMID 20457603.
          user_report Wang H.Y., Transcriptional regulation of human eosinophil RNases by an evolutionary- conserved sequence motif in primate genome. BMC Mol. Biol., 8:89 (2007). PMID 17927842.

          2022.05.18

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