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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pGEX-hMAZ

Expression vector of human MAZ in E. coli

Catalog number RDB05701
Resource name pGEX-hMAZ
Clone info. Expression vector of human MAZ in E. coli.
This clone contains a cDNA fragment corresponding to the 174 to 1607nt of NM_002383.4. Known differences (at least): 15nt (ggcggcagcggcagc) deletion at 1493 to 1507nt.
Vector backbone pGEX-4T-2 (Plasmid)
Size of vector backbone 4.97 kb
Selectable markers Amp^r
Gene/insert name Human MYC-associated zinc finger protein (purine-binding transcription factor) cDNA
Depositor|Developer Yokoyama, Kazunari | Song, Jun |
 
Other clones in our bank human MAZ (NCBI Gene 4150) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR Manuscripts for the publication shall be made through the discussion between Depositor and Recipient Investigator. Recipient investigator shall include Depositor/Developer as co-author in manuscript for the publication. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Biochem. Biophys. Res. Commun., 226, 801-809 (1996).) Recipient Investigator shall negotiate with Developer/Depositor when patent application and the commercialization for the products based upon Material, derivatives thereof their use is considered.
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RDB05701 pGEX-hMAZ DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

original Song, J., Two consecutive zinc fingers in Sp1 and in MAZ are essential for interactions with cis-elements. J. Biol. Chem., 276, 30429-30434 (2001). PMID 11395515.

Further references such as user reports and related articles (go to bottom)

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB18977_B1Dkp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pGEX-F2
Region: GST,thronbin site,insert 5'
Sequence file: RDB18977_B1Dka.seq check
>05701_18977_B1Dk_3_pGEX-F2_A01_01_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNTATTGNA GCTATCCCAC AAATTNNATA AGTACTTGAA
   61 ATCCAGCAAG TATATAGCAT GGCCTTTGCA GGGCTGGCAA GCCACGTTTG GTGGTGGCGA
  121 CCATCCTCCA AAATCGGATC TGGTTCCGCG TGGATCCCCA GGAATTCTGC AGTCGACGGT
  181 ACCGGGCCCC GCTGCGGCCG AGGCCATGTT CCCGGTGTTT CCTTGCACGC TGCTGGCCCC
  241 CCCCTTCCCC GTGCTGGGCC TGGACTCCCG GGGGGTGGGC GGCCTCATGA ACTCCTTCCC
  301 GCCACCTCAG GGTCACGCCC AGAACCCCCT GCAGGTCGGG GCTGAGCTCC AGTCCCGCTT
  361 CTTTGCCTCC CAGGGCTGCG CCCAGAGTCC ATTCCAGGCC GCGCCGGCGC CCCCGCCCAC
  421 GCCCCAGGCC CCGGCGGCCG AGCCCCTCCA GGTGGACTTG CTCCCGGTGC TCGCCGCCGC
  481 CCAGGAGTCC GCCGCGGCTG CTGCGGCCGC TGCCGCCGCT GCTGCCGCCG TCGCTGCCGC
  541 GCCCCCGGCC CCTGCCGCCG CCTCTACGGT GGACACAGCG GCCCTGAAGC AGCCTCCGGC
  601 GCCCCCTCCG CCACCCCCGC CAGTGTCGGC GCCCGCGGCC GANGCCGCGC CCCCCGCCTC
  661 CGCCGCCACT ATCGCCGCGG CGGCGGNCNN CGCNNNCNNA NCCCCNNNCT NNNNNNNNNN
//
Primer: pJB8-Forward
Region: insert 3'
Sequence file: RDB18977_B1Dkb.seq check
>05701_18977_B1Dk_3_pJB8-Forward_B01_04_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNG TNNTGGCTGC GCCCCGACAC CCGCCAACAC CCGCTGACGC
   61 GCCCTGACGG GCTTGTCTGC TCCCGGCATC CGCTTACAGA CAAGCTGTGA CCGTCTCCGG
  121 GAGCTGCATG TGTCAGAGGT TTTCACCGTC ATCACCGAAA CGCGCGAGGC AGATCGTCAG
  181 TCAGTCACGA TGCGGCCGCT CGAGTCGACC CGGGAATTCC CCTAAGAAAA CATTTCTTCC
  241 TCCTTTCCTT CTGGAGGCTC CTTGGTTGGT AGGGAAATAG GAGTTGGTGG GAAAAGAGGG
  301 GGGAGAGGAG CTTGTACCAA GGGACTCTAC TCCATTCTCC CCTCTCCCCC GCAACCAACT
  361 TGGAGCTCAC CAGGGTTGGG AGGGAAGTGG CTGAGAGCTC ACAGGCACCC CCTCCGCCCC
  421 CGAGAGGGAG CCCACAGCTG TGGGAGGGGC TGCTACTGCT GCCGCTGCTG CCGCTGCCGC
  481 CGCCATTGGA CAAACCTCAC CAGTACCTTT GTTGCAGAGC TCACAGACAT GGTGAGGACC
  541 CTGGCTGTGC ACCTTCATGT GGTCCGAAAT ATAAGCCGAG CTCAGCATCT TGCCACACAC
  601 GTGACATGGC ACTTTCTCCT CGTGTCGNAC TGTGTGCGCC CGCAGCCGAT CCTTCNTGGC
  661 GAANGCTGCC TCACATTTCT CACATTTGAA NGNCCNNTCN GNNNAGNGNA CNTNNNCNNN
721 NNNNNNN
//
Primer: lacIpro-B_F
Region: lacI pro,lacI
Sequence file: RDB18977_B1Dkc.seq check
>05701_18977_B1Dk_3_laclpro-B_F_C01_07_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNTCNGTGNN CGANGAANCG GAAGANCGCC TGNTGCGGTA
   61 TTTTCTCCTT ACGCATCTGT GCGGTATTTC ACACCGCATA AATTCCGACA CCATCGAATG
  121 GCGCAAAACC TTTCGCGGTA TGGCATGATA GCGCCCGGAA GAGAGTCAAT TCAGGGTGGT
  181 GAATGTGAAA CCAGTAACGT TATACGATGT CGCAGAGTAT GCCGGTGTCT CTTATCAGAC
  241 CGTTTCCCGC GTGGTGAACC AGGCCAGCCA CGTTTCTGCG AAAACGCGGG AAAAAGTGGA
  301 AGCGGCGATG GCGGAGCTGA ATTACATTCC CAACCGCGTG GCACAACAAC TGGCGGGCAA
  361 ACAGTCGTTG CTGATTGGCG TTGCCACCTC CAGTCTGGCC CTGCACGCGC CGTCGCAAAT
  421 TGTCGCGGCG ATTAAATCTC GCGCCGATCA ACTGGGTGCC AGCGTGGTGG TGTCGATGGT
  481 AGAACGAAGC GGCGTCGAAG CCTGTAAAGC GGCGGTGCAC AATCTTCTCG CGCAACGCGT
  541 CAGTGGGCTG ATCATTAACT ATCCGCTGGA TGACCAGGAT GCCATTGCTG TGGAAGCTGC
  601 CTGCACTAAT GTTCCGGCGT TATTTCTTGA TGTCTCTGAN CAGACACCCA TCNACAGTAT
661 TANTTTCTNC CNTGNNGNNN NNCNCNNCTG GNNNNNNNNN N
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Song, J., Two consecutive zinc fingers in Sp1 and in MAZ are essential for interactions with cis-elements. J. Biol. Chem., 276, 30429-30434 (2001). PMID 11395515.
user report Cogoi, S., MAZ-binding G4-decoy with locked nucleic acid and twisted intercalating nucleic acid modifications suppresses KRAS in pancreatic cancer cells and delays tumor growth in mice. Nucleic Acids Res. 2013 Apr;41(7):4049-64. PMID 23471001.
user report Membrino, A., G4-DNA Formation in the HRAS Promoter and Rational Design of Decoy Oligonucleotides for Cancer Therapy. PLoS One. 6 (9): e24421 (2011). PMID 21931711.
user report Cogoi S., The KRAS promoter responds to Myc-associated zinc finger and poly(ADP-ribose) polymerase 1 proteins, which recognize a critical quadruplex-forming GA-element. J. Biol. Chem., 285, 22003-22016 (2010). PMID 20457603.
user report Wang H.Y., Transcriptional regulation of human eosinophil RNases by an evolutionary- conserved sequence motif in primate genome. BMC Mol. Biol., 8:89 (2007). PMID 17927842.

2021.06.03

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