Resource data sheet
Prev.

pGEX-hMAZ

Expression vector of human MAZ in E. coli

Catalog number RDB05701
Resource name pGEX-hMAZ
Clone info. Expression vector of human MAZ in E. coli.
Vector backbone pGEX-4T-2 (Plasmid)
Size of vector backbone 4.97 kb
Selectable markers Amp^r
Gene/insert name Human MYC-associated zinc finger protein (purine-binding transcription factor) cDNA
Depositor Yokoyama, Kazunari | Song, Jun |
 
Other clones in our bank human MAZ (NCBI Gene 4150) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering Information [in Japanese] [in English]
Terms and conditions set forth by the DEPOSITOR Manuscripts for the publication shall be made through the discussion between Depositor and Recipient Investigator. Recipient investigator shall include Depositor/Developer as co-author in manuscript for the publication. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Biochem. Biophys. Res. Commun., 226, 801-809 (1996).) Recipient Investigator shall negotiate with Developer/Depositor when patent application and the commercialization for the products based upon Material, derivatives thereof their use is considered.
(Japanese text [open/close])

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB05701 pGEX-hMAZ DNA solution

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB12834_A4Gp.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pGEX_F RDB12834_A4Gpa.seq
>05701_12834_A4Gp_pGEX_F_B01_04.ab1
    1 GGCATCATCA ATCGGATCTG GTTCCGCGTG GATCCCCAGG AATTCTGCAG TCGACGGTAC
   61 CGGGCCCCGC TGCGGCCGAG GCCATGTTCC CGGTGTTTCC TTGCACGCTG CTGGCCCCCC
  121 CCTTCCCCGT GCTGGGCCTG GACTCCCGGG GGGTGGGCGG CCTCATGAAC TCCTTCCCGC
  181 CACCTCAGGG TCACGCCCAG AACCCCCTGC AGGTCGGGGC TGAGCTCCAG TCCCGCTTCT
  241 TTGCCTCCCA GGGCTGCGCC CAGAGTCCAT TCCAGGCCGC GCCGGCGCCC CCGCCCACGC
  301 CCCAGGCCCC GGCGGCCGAG CCCCTCCAGG TGGACTTGCT CCCGGTGCTC GCCGCCGCCC
  361 AGGAGTCCGC CGCGGCTGCT GCGGCCGCTG CCGCCGCTGC TGCCGCCGTC GCTGCCGCGC
  421 CCCCGGCCCC TGCCGCCGCC TCTACGGTGG ACACAGCGGC CCTGAAGCAG CCTCCGGCGC
  481 CCCCTCCGCC ACCCCCGCCA GTGTCGGCGC CCGCGGCCGA GGCCGCGCCC CCCGCCTCCG
  541 CCGCCACTAT CGCCGCGGCG GCGGCCACCG CCGTCGTAGC CCCAACCTCG ACGGTCGCCG
  601 TGGCCCCGGT CGCGTCTGCC TTGGAGAAGA AGACAAAGAG CAAGGGGCCC TACATCTGCG
  661 CTCTGTGCGC CAAGGAGTTC AAGAACGGCT ACAATCTCCG GAGGCACGAA GCCATCCACA
  721 CGGGAGCCAA GGCCGGCCGG GTCCCCTCGG GTGCTATGAA GATGCCGACC ATGGTGCCCC
  781 TGAGCCTCCT GAGCGTGCCC CAGCTGAGCG GAGCCGGCGG GGGAGGGGGA GAGGCGGGTG
  841 CCGGCGGCGG CGCTGCCGCA GTGGCCGCCG GTGGCGTGGT GACCACGACC GCCTCGGGGA
  901 AGCGCATCCG GAAGAACCAT GCCTGCGAGA TGTGTGGCAA GGCCTTCCGC GACGTCTACC
  961 ACCTGAACCG ACACAAGCTG TCGCACTCGG ACGAGAAGCC CTACCAGTGC CCGGTGTGCC
 1021 CAGCAGCGCT TCAAGCGCAA AGGACCGCAA TGAGCTACCA CGTGCGCCTC ACATGAACGC
1081 GCTATGGCAC AAGCCTCTAC CACTACTCCC CAACTG
//
Primer: pGEX_R RDB12834_A4Gpb.seq
>05701_12834_A4Gp_pGEX_R_B02_05.ab1
    1 TGGGGGGGCT GTCATCCCGA ACGCGCGAGG CAGATCGTCA GTCAGTCACG ACCGCGGCCG
   61 CTCGAGTCGA CCCGGGAATT CCCCAAAAAA ACATTTCTTC CTCCTTTCCT TCTGGAGGCT
  121 CCTTGGTTGG TAGGGAAATA GGAGTTGGTG GGAAAAGAGG GGGGAGAGGA GCTTGTACCA
  181 AGGGACTCTA CTCCATTCTC CCCTCTCCCC CGCAACCAAC TTGGAGCTCA CCAGGGTTGG
  241 GAGGGAAGTG GCTGAGAGCT CACAGGCACC CCCTCCGCCC CCGAGAGGGA GCCCACAGCT
  301 GTGGGAGGGG CTGCTACTGC TGCCGCTGCT GCCGCTGCCG CCGCCATTGG ACAAACCTCA
  361 CCAGTACCTT TGTTGCAGAG CTCACAGACA TGGTGAGGAC CCTGGCTGTG CACCTTCATG
  421 TGGTCCGAAA TATAAGCCGA GCTCAGCATC TTGCCACACA CGTGACATGG CACTTTCTCC
  481 TCGTGTCGTA CTGTGTGCGC CCGCAGCCGA TCCTTCGTGG CGAAAGCTGC CTCACATTTC
  541 TCACATTTGA AGGGCCGTTC TGTTGAGTGC ACTTGTCTGA CGTGACTGTT GAGGTGATCC
  601 GGCCGGGAGA AGCTCTTGCC ACAGTGGGAG CAGTTGTAGG GCTTGTGCAC AGCGCCGTCA
  661 TGTGAGCGCA CGTGGTAGCT CATGCGGTCC TTGCGCTTGA AGCGCTGCTG GCACACCGGG
  721 CACTGGTAGG GCTTCTCGTC CGAGTGCGAC AGCTTGTGTC GGTTCAGGTG GTAGACGTCG
  781 CGGAAGGCCT TGCCACACAT CTCGCAGGCA TGGTTCTTCC GGATGCGCTT CCCCGAGGCG
  841 GTCGTGGTCA CCACGCCCAC CGGCGGCCAC TGCCGGCAGC GCCGCCCGCC GGGCACCCGC
  901 CTCTCCCCCT CCCCCGCCGG CTCCGCTTCA GCTTGGGGCA CCGCTTCATG AAGGCTCAGG
  961 GGCACCATGG TCGGCATTCT TCATAGCACC CGAAGGGACC CGGCCCGGCC TTGGCTTCCC
 1021 GTGTGATGGC TTCGTGCCTC CGGAAGATTG TAACCCGTCC TTGAACTTCC TTTGGCGCAC
 1081 AGAATCGCAC ATGTAGGGCC CCCTTGCTCC TTTTGGTCTT CTATCTCCAT GGTATAACGT
 1141 CCAACCTGGG TCTACAGCGG AACCCGTCTG AGGTTTGGTG CCACTCACTC ACTGGTCCAG
1201 GTGTAGCGTC ACGCTGCACG GCTCTCA
//
Primer: MAZ-M_R2 RDB12834_A4Gpc.seq
>05701_12834_A4Gp_MAZ-M_R2_A01_01.ab1
    1 AGCGATATGT GAGCGCACGT GGTAGCTCAT GCGGTCCTTG CGCTTGAAGC GCTGCTGGCA
   61 CACCGGGCAC TGGTAGGGCT TCTCGTCCGA GTGCGACAGC TTGTGTCGGT TCAGGTGGTA
  121 GACGTCGCGG AAGGCCTTGC CACACATCTC GCAGGCATGG TTCTTCCGGA TGCGCTTCCC
  181 CGAGGCGGTC GTGGTCACCA CGCCACCGGC GGCCACTGCG GCAGCGCCGC CGCCGGCACC
  241 CGCCTCTCCC CCTCCCCCGC CGGCTCCGCT CAGCTGGGGC ACGCTCAGGA GGCTCAGGGG
  301 CACCATGGTC GGCATCTTCA TAGCACCCGA GGGGACCCGG CCGGCCTTGG CTCCCGTGTG
  361 GATGGCTTCG TGCCTCCGGA GATTGTAGCC GTTCTTGAAC TCCTTGGCGC ACAGAGCGCA
  421 GATGTAGGGC CCCTTGCTCT TTGTCTTCTT CTCCAAGGCA GACGCGACCG GGGCCACGGC
  481 GACCGTCGAG GTTGGGGCTA CGACGGCGGT GGCCGCCGCC GCGGCGATAG TGGCGGCGGA
  541 GGCGGGGGGC GCGGCCTCGG CCGCGGGCGC CGACACTGGC GGGGGTGGCG GAGGGGGCGC
  601 CGGAGGCTGC TTCAGGGCCG CTGTGTCCAC CGTAGAGGCG GCGGCAGGGG CCGGGGGCGC
  661 GGCAGCGACG GCGGCAGCAG CGGCGGCAGC GGCCGCAGCA GCCGCGGCGG ACTCCTGGGC
  721 GGCGGCGAGC ACCGGGAGCA AGTCCACCTG GAGGGGCTCG GCCGCCGGGG CCTGGGGCGT
  781 GGGCGGGGGC GCCGGCGCGG CCTGGAATGG ACTCTGGGCG CAGCCCTGGG AGGCAAAGAA
  841 GCGGGACTGG AGCTCAGCCC CGACCTGCAG GGGGTTCTGG GCGTGACCCT GAGGTGGCGG
  901 GAAGGAGTTC ATGAGGCCGC CCACCCCCCC GGGAGTCCAG GCCCAGCACG GGGAAAGGGG
  961 GGGGCCAGCA GCGTGCAAGG AAACACCGGG AACATGGGCC TCGGCCGCAG CGGGGCCCGG
 1021 GTACCGTCGA CTGCAGATTC CCTGGGGGAT CCACGCGGGA CCAGAATCGG AATTTTGGAA
 1081 GAATGGATCG GCCACAGAAA CGTTGCCTGG CAGGCCTTGC AAGGTCATGC TAATAATACT
1141 G
//

Please visit plasmidSequencing and PCR primers for primer information.

References and tips

Electronic file

Featured content

References

original , J. Biol. Chem., 276, 30429-30434 (2001). PMID 11395515.
user report Cogoi, S., MAZ-binding G4-decoy with locked nucleic acid and twisted intercalating nucleic acid modifications suppresses KRAS in pancreatic cancer cells and delays tumor growth in mice. Nucleic Acids Res. 2013 Apr;41(7):4049-64. PMID 23471001.
user report Membrino, A., G4-DNA Formation in the HRAS Promoter and Rational Design of Decoy Oligonucleotides for Cancer Therapy. PLoS One. 6 (9): e24421 (2011). PMID 21931711.
user report Cogoi S., The KRAS promoter responds to Myc-associated zinc finger and poly(ADP-ribose) polymerase 1 proteins, which recognize a critical quadruplex-forming GA-element. J. Biol. Chem., 285, 22003-22016 (2010). PMID 20457603.
user report Wang H.Y., Transcriptional regulation of human eosinophil RNases by an evolutionary- conserved sequence motif in primate genome. BMC Mol. Biol., 8:89 (2007). PMID 17927842.

2017.10.23

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