RDB05701 - pGEX-hMAZ

Resource data sheet
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pGEX-hMAZ

Expression vector of human MAZ in E. coli


Catalog number	RDB05701
Resource name	pGEX-hMAZ
Clone info.	Expression vector of human MAZ in E. coli.
Vector backbone	pGEX-4T-2 (Plasmid)
Size of vector backbone	4.97 kb
Selectable markers	Amp^r
Gene/insert name	Human MYC-associated zinc finger protein (purine-binding transcription factor) cDNA
Depositor	Yokoyama, Kazunari \| Song, Jun \|
Other clones in our bank	human MAZ (NCBI Gene 4150) \|

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR Manuscripts for the publication shall be made through the discussion between Depositor and Recipient Investigator. Recipient investigator shall include Depositor/Developer as co-author in manuscript for the publication. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Biochem. Biophys. Res. Commun., 226, 801-809 (1996).) Recipient Investigator shall negotiate with Developer/Depositor when patent application and the commercialization for the products based upon Material, derivatives thereof their use is considered.

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Distribution information (domestic users) [open/close]

Terms and conditions set forth by the DEPOSITOR	Manuscripts for the publication shall be made through the discussion between Depositor and Recipient Investigator. Recipient investigator shall include Depositor/Developer as co-author in manuscript for the publication. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Biochem. Biophys. Res. Commun., 226, 801-809 (1996).) Recipient Investigator shall negotiate with Developer/Depositor when patent application and the commercialization for the products based upon Material, derivatives thereof their use is considered.
Ordering	Please visit Information of Request for Distribution. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word]

提供条件	使用にあたっては常に密な連絡をとり、発表等に関しても事前に合意を要する。共著とする。利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Biochem. Biophys. Res. Commun., 226, 801-809 (1996).)。本リソースを使用して商業利用する場合は、寄託者と改めて契約する。
提供依頼	手続きの詳細は、「提供申込みについて」をご覧ください。提供依頼書 [Word] 提供同意書 [Word]

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB05701	pGEX-hMAZ	DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet	RDB12834_A4Gp.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pGEX_F	RDB12834_A4Gpa.seq
>05701_12834_A4Gp_pGEX_F_B01_04.ab1 1 GGCATCATCA ATCGGATCTG GTTCCGCGTG GATCCCCAGG AATTCTGCAG TCGACGGTAC 61 CGGGCCCCGC TGCGGCCGAG GCCATGTTCC CGGTGTTTCC TTGCACGCTG CTGGCCCCCC 121 CCTTCCCCGT GCTGGGCCTG GACTCCCGGG GGGTGGGCGG CCTCATGAAC TCCTTCCCGC 181 CACCTCAGGG TCACGCCCAG AACCCCCTGC AGGTCGGGGC TGAGCTCCAG TCCCGCTTCT 241 TTGCCTCCCA GGGCTGCGCC CAGAGTCCAT TCCAGGCCGC GCCGGCGCCC CCGCCCACGC 301 CCCAGGCCCC GGCGGCCGAG CCCCTCCAGG TGGACTTGCT CCCGGTGCTC GCCGCCGCCC 361 AGGAGTCCGC CGCGGCTGCT GCGGCCGCTG CCGCCGCTGC TGCCGCCGTC GCTGCCGCGC 421 CCCCGGCCCC TGCCGCCGCC TCTACGGTGG ACACAGCGGC CCTGAAGCAG CCTCCGGCGC 481 CCCCTCCGCC ACCCCCGCCA GTGTCGGCGC CCGCGGCCGA GGCCGCGCCC CCCGCCTCCG 541 CCGCCACTAT CGCCGCGGCG GCGGCCACCG CCGTCGTAGC CCCAACCTCG ACGGTCGCCG 601 TGGCCCCGGT CGCGTCTGCC TTGGAGAAGA AGACAAAGAG CAAGGGGCCC TACATCTGCG 661 CTCTGTGCGC CAAGGAGTTC AAGAACGGCT ACAATCTCCG GAGGCACGAA GCCATCCACA 721 CGGGAGCCAA GGCCGGCCGG GTCCCCTCGG GTGCTATGAA GATGCCGACC ATGGTGCCCC 781 TGAGCCTCCT GAGCGTGCCC CAGCTGAGCG GAGCCGGCGG GGGAGGGGGA GAGGCGGGTG 841 CCGGCGGCGG CGCTGCCGCA GTGGCCGCCG GTGGCGTGGT GACCACGACC GCCTCGGGGA 901 AGCGCATCCG GAAGAACCAT GCCTGCGAGA TGTGTGGCAA GGCCTTCCGC GACGTCTACC 961 ACCTGAACCG ACACAAGCTG TCGCACTCGG ACGAGAAGCC CTACCAGTGC CCGGTGTGCC 1021 CAGCAGCGCT TCAAGCGCAA AGGACCGCAA TGAGCTACCA CGTGCGCCTC ACATGAACGC 1081 GCTATGGCAC AAGCCTCTAC CACTACTCCC CAACTG //
Primer: pGEX_R	RDB12834_A4Gpb.seq
>05701_12834_A4Gp_pGEX_R_B02_05.ab1 1 TGGGGGGGCT GTCATCCCGA ACGCGCGAGG CAGATCGTCA GTCAGTCACG ACCGCGGCCG 61 CTCGAGTCGA CCCGGGAATT CCCCAAAAAA ACATTTCTTC CTCCTTTCCT TCTGGAGGCT 121 CCTTGGTTGG TAGGGAAATA GGAGTTGGTG GGAAAAGAGG GGGGAGAGGA GCTTGTACCA 181 AGGGACTCTA CTCCATTCTC CCCTCTCCCC CGCAACCAAC TTGGAGCTCA CCAGGGTTGG 241 GAGGGAAGTG GCTGAGAGCT CACAGGCACC CCCTCCGCCC CCGAGAGGGA GCCCACAGCT 301 GTGGGAGGGG CTGCTACTGC TGCCGCTGCT GCCGCTGCCG CCGCCATTGG ACAAACCTCA 361 CCAGTACCTT TGTTGCAGAG CTCACAGACA TGGTGAGGAC CCTGGCTGTG CACCTTCATG 421 TGGTCCGAAA TATAAGCCGA GCTCAGCATC TTGCCACACA CGTGACATGG CACTTTCTCC 481 TCGTGTCGTA CTGTGTGCGC CCGCAGCCGA TCCTTCGTGG CGAAAGCTGC CTCACATTTC 541 TCACATTTGA AGGGCCGTTC TGTTGAGTGC ACTTGTCTGA CGTGACTGTT GAGGTGATCC 601 GGCCGGGAGA AGCTCTTGCC ACAGTGGGAG CAGTTGTAGG GCTTGTGCAC AGCGCCGTCA 661 TGTGAGCGCA CGTGGTAGCT CATGCGGTCC TTGCGCTTGA AGCGCTGCTG GCACACCGGG 721 CACTGGTAGG GCTTCTCGTC CGAGTGCGAC AGCTTGTGTC GGTTCAGGTG GTAGACGTCG 781 CGGAAGGCCT TGCCACACAT CTCGCAGGCA TGGTTCTTCC GGATGCGCTT CCCCGAGGCG 841 GTCGTGGTCA CCACGCCCAC CGGCGGCCAC TGCCGGCAGC GCCGCCCGCC GGGCACCCGC 901 CTCTCCCCCT CCCCCGCCGG CTCCGCTTCA GCTTGGGGCA CCGCTTCATG AAGGCTCAGG 961 GGCACCATGG TCGGCATTCT TCATAGCACC CGAAGGGACC CGGCCCGGCC TTGGCTTCCC 1021 GTGTGATGGC TTCGTGCCTC CGGAAGATTG TAACCCGTCC TTGAACTTCC TTTGGCGCAC 1081 AGAATCGCAC ATGTAGGGCC CCCTTGCTCC TTTTGGTCTT CTATCTCCAT GGTATAACGT 1141 CCAACCTGGG TCTACAGCGG AACCCGTCTG AGGTTTGGTG CCACTCACTC ACTGGTCCAG 1201 GTGTAGCGTC ACGCTGCACG GCTCTCA //
Primer: MAZ-M_R2	RDB12834_A4Gpc.seq
>05701_12834_A4Gp_MAZ-M_R2_A01_01.ab1 1 AGCGATATGT GAGCGCACGT GGTAGCTCAT GCGGTCCTTG CGCTTGAAGC GCTGCTGGCA 61 CACCGGGCAC TGGTAGGGCT TCTCGTCCGA GTGCGACAGC TTGTGTCGGT TCAGGTGGTA 121 GACGTCGCGG AAGGCCTTGC CACACATCTC GCAGGCATGG TTCTTCCGGA TGCGCTTCCC 181 CGAGGCGGTC GTGGTCACCA CGCCACCGGC GGCCACTGCG GCAGCGCCGC CGCCGGCACC 241 CGCCTCTCCC CCTCCCCCGC CGGCTCCGCT CAGCTGGGGC ACGCTCAGGA GGCTCAGGGG 301 CACCATGGTC GGCATCTTCA TAGCACCCGA GGGGACCCGG CCGGCCTTGG CTCCCGTGTG 361 GATGGCTTCG TGCCTCCGGA GATTGTAGCC GTTCTTGAAC TCCTTGGCGC ACAGAGCGCA 421 GATGTAGGGC CCCTTGCTCT TTGTCTTCTT CTCCAAGGCA GACGCGACCG GGGCCACGGC 481 GACCGTCGAG GTTGGGGCTA CGACGGCGGT GGCCGCCGCC GCGGCGATAG TGGCGGCGGA 541 GGCGGGGGGC GCGGCCTCGG CCGCGGGCGC CGACACTGGC GGGGGTGGCG GAGGGGGCGC 601 CGGAGGCTGC TTCAGGGCCG CTGTGTCCAC CGTAGAGGCG GCGGCAGGGG CCGGGGGCGC 661 GGCAGCGACG GCGGCAGCAG CGGCGGCAGC GGCCGCAGCA GCCGCGGCGG ACTCCTGGGC 721 GGCGGCGAGC ACCGGGAGCA AGTCCACCTG GAGGGGCTCG GCCGCCGGGG CCTGGGGCGT 781 GGGCGGGGGC GCCGGCGCGG CCTGGAATGG ACTCTGGGCG CAGCCCTGGG AGGCAAAGAA 841 GCGGGACTGG AGCTCAGCCC CGACCTGCAG GGGGTTCTGG GCGTGACCCT GAGGTGGCGG 901 GAAGGAGTTC ATGAGGCCGC CCACCCCCCC GGGAGTCCAG GCCCAGCACG GGGAAAGGGG 961 GGGGCCAGCA GCGTGCAAGG AAACACCGGG AACATGGGCC TCGGCCGCAG CGGGGCCCGG 1021 GTACCGTCGA CTGCAGATTC CCTGGGGGAT CCACGCGGGA CCAGAATCGG AATTTTGGAA 1081 GAATGGATCG GCCACAGAAA CGTTGCCTGG CAGGCCTTGC AAGGTCATGC TAATAATACT 1141 G //

Please visit Sequencing and PCR primers for primer information.

References

original	, J. Biol. Chem., 276, 30429-30434 (2001). PMID 11395515.
user report	Cogoi, S., MAZ-binding G4-decoy with locked nucleic acid and twisted intercalating nucleic acid modifications suppresses KRAS in pancreatic cancer cells and delays tumor growth in mice. Nucleic Acids Res. 2013 Apr;41(7):4049-64. PMID 23471001.
user report	Membrino, A., G4-DNA Formation in the HRAS Promoter and Rational Design of Decoy Oligonucleotides for Cancer Therapy. PLoS One. 6 (9): e24421 (2011). PMID 21931711.
user report	Cogoi S., The KRAS promoter responds to Myc-associated zinc finger and poly(ADP-ribose) polymerase 1 proteins, which recognize a critical quadruplex-forming GA-element. J. Biol. Chem., 285, 22003-22016 (2010). PMID 20457603.
user report	Wang H.Y., Transcriptional regulation of human eosinophil RNases by an evolutionary- conserved sequence motif in primate genome. BMC Mol. Biol., 8:89 (2007). PMID 17927842.

2018.12.11

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