RDB05701 - pGEX-hMAZ

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pGEX-hMAZ

Expression vector of human MAZ in E. coli


Catalog number	RDB05701
Resource name	pGEX-hMAZ
Clone info.	Expression vector of human MAZ in E. coli. This clone contains a cDNA fragment corresponding to the 174 to 1607nt of NM_002383.4. Known differences (at least): 15nt (ggcggcagcggcagc) deletion at 1493 to 1507nt.
Vector backbone	pGEX-4T-2 (Plasmid)
Size of vector backbone	4.97 kb
Selectable markers	Amp^r
Gene/insert name	Human MYC-associated zinc finger protein (purine-binding transcription factor) cDNA
Depositor\|Developer	Yokoyama, Kazunari \| Song, Jun \|
Other clones in our bank	human MAZ (NCBI Gene 4150) \|

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR Manuscripts for the publication shall be made through the discussion between Depositor and Recipient Investigator. Recipient investigator shall include Depositor/Developer as co-author in manuscript for the publication. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Biochem. Biophys. Res. Commun., 226, 801-809 (1996).) Recipient Investigator shall negotiate with Developer/Depositor when patent application and the commercialization for the products based upon Material, derivatives thereof their use is considered.

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Distribution information (domestic users) [open/close]

Terms and conditions set forth by the DEPOSITOR	Manuscripts for the publication shall be made through the discussion between Depositor and Recipient Investigator. Recipient investigator shall include Depositor/Developer as co-author in manuscript for the publication. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Biochem. Biophys. Res. Commun., 226, 801-809 (1996).) Recipient Investigator shall negotiate with Developer/Depositor when patent application and the commercialization for the products based upon Material, derivatives thereof their use is considered.
Ordering	Please visit Information of Request for Distribution.[link] Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

提供条件	使用にあたっては常に密な連絡をとり、発表等に関しても事前に合意を要する。共著とする。利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Biochem. Biophys. Res. Commun., 226, 801-809 (1996).)。本リソースを使用して商業利用する場合は、寄託者と改めて契約する。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB05701	pGEX-hMAZ	DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check

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References and tips

Electronic file

Original reference

original	Song, J., Two consecutive zinc fingers in Sp1 and in MAZ are essential for interactions with cis-elements. J. Biol. Chem., 276, 30429-30434 (2001). PMID 11395515.

Further references such as user reports and related articles (go to bottom)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB18977_B1Dkp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pGEX-F2 Region: GST,thronbin site,insert 5' Sequence file: RDB18977_B1Dka.seq
>05701_18977_B1Dk_3_pGEX-F2_A01_01_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNTATTGNA GCTATCCCAC AAATTNNATA AGTACTTGAA 61 ATCCAGCAAG TATATAGCAT GGCCTTTGCA GGGCTGGCAA GCCACGTTTG GTGGTGGCGA 121 CCATCCTCCA AAATCGGATC TGGTTCCGCG TGGATCCCCA GGAATTCTGC AGTCGACGGT 181 ACCGGGCCCC GCTGCGGCCG AGGCCATGTT CCCGGTGTTT CCTTGCACGC TGCTGGCCCC 241 CCCCTTCCCC GTGCTGGGCC TGGACTCCCG GGGGGTGGGC GGCCTCATGA ACTCCTTCCC 301 GCCACCTCAG GGTCACGCCC AGAACCCCCT GCAGGTCGGG GCTGAGCTCC AGTCCCGCTT 361 CTTTGCCTCC CAGGGCTGCG CCCAGAGTCC ATTCCAGGCC GCGCCGGCGC CCCCGCCCAC 421 GCCCCAGGCC CCGGCGGCCG AGCCCCTCCA GGTGGACTTG CTCCCGGTGC TCGCCGCCGC 481 CCAGGAGTCC GCCGCGGCTG CTGCGGCCGC TGCCGCCGCT GCTGCCGCCG TCGCTGCCGC 541 GCCCCCGGCC CCTGCCGCCG CCTCTACGGT GGACACAGCG GCCCTGAAGC AGCCTCCGGC 601 GCCCCCTCCG CCACCCCCGC CAGTGTCGGC GCCCGCGGCC GANGCCGCGC CCCCCGCCTC 661 CGCCGCCACT ATCGCCGCGG CGGCGGNCNN CGCNNNCNNA NCCCCNNNCT NNNNNNNNNN //
Primer: pJB8-Forward Region: insert 3' Sequence file: RDB18977_B1Dkb.seq
>05701_18977_B1Dk_3_pJB8-Forward_B01_04_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNG TNNTGGCTGC GCCCCGACAC CCGCCAACAC CCGCTGACGC 61 GCCCTGACGG GCTTGTCTGC TCCCGGCATC CGCTTACAGA CAAGCTGTGA CCGTCTCCGG 121 GAGCTGCATG TGTCAGAGGT TTTCACCGTC ATCACCGAAA CGCGCGAGGC AGATCGTCAG 181 TCAGTCACGA TGCGGCCGCT CGAGTCGACC CGGGAATTCC CCTAAGAAAA CATTTCTTCC 241 TCCTTTCCTT CTGGAGGCTC CTTGGTTGGT AGGGAAATAG GAGTTGGTGG GAAAAGAGGG 301 GGGAGAGGAG CTTGTACCAA GGGACTCTAC TCCATTCTCC CCTCTCCCCC GCAACCAACT 361 TGGAGCTCAC CAGGGTTGGG AGGGAAGTGG CTGAGAGCTC ACAGGCACCC CCTCCGCCCC 421 CGAGAGGGAG CCCACAGCTG TGGGAGGGGC TGCTACTGCT GCCGCTGCTG CCGCTGCCGC 481 CGCCATTGGA CAAACCTCAC CAGTACCTTT GTTGCAGAGC TCACAGACAT GGTGAGGACC 541 CTGGCTGTGC ACCTTCATGT GGTCCGAAAT ATAAGCCGAG CTCAGCATCT TGCCACACAC 601 GTGACATGGC ACTTTCTCCT CGTGTCGNAC TGTGTGCGCC CGCAGCCGAT CCTTCNTGGC 661 GAANGCTGCC TCACATTTCT CACATTTGAA NGNCCNNTCN GNNNAGNGNA CNTNNNCNNN 721 NNNNNNN //
Primer: lacIpro-B_F Region: lacI pro,lacI Sequence file: RDB18977_B1Dkc.seq
>05701_18977_B1Dk_3_laclpro-B_F_C01_07_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNTCNGTGNN CGANGAANCG GAAGANCGCC TGNTGCGGTA 61 TTTTCTCCTT ACGCATCTGT GCGGTATTTC ACACCGCATA AATTCCGACA CCATCGAATG 121 GCGCAAAACC TTTCGCGGTA TGGCATGATA GCGCCCGGAA GAGAGTCAAT TCAGGGTGGT 181 GAATGTGAAA CCAGTAACGT TATACGATGT CGCAGAGTAT GCCGGTGTCT CTTATCAGAC 241 CGTTTCCCGC GTGGTGAACC AGGCCAGCCA CGTTTCTGCG AAAACGCGGG AAAAAGTGGA 301 AGCGGCGATG GCGGAGCTGA ATTACATTCC CAACCGCGTG GCACAACAAC TGGCGGGCAA 361 ACAGTCGTTG CTGATTGGCG TTGCCACCTC CAGTCTGGCC CTGCACGCGC CGTCGCAAAT 421 TGTCGCGGCG ATTAAATCTC GCGCCGATCA ACTGGGTGCC AGCGTGGTGG TGTCGATGGT 481 AGAACGAAGC GGCGTCGAAG CCTGTAAAGC GGCGGTGCAC AATCTTCTCG CGCAACGCGT 541 CAGTGGGCTG ATCATTAACT ATCCGCTGGA TGACCAGGAT GCCATTGCTG TGGAAGCTGC 601 CTGCACTAAT GTTCCGGCGT TATTTCTTGA TGTCTCTGAN CAGACACCCA TCNACAGTAT 661 TANTTTCTNC CNTGNNGNNN NNCNCNNCTG GNNNNNNNNN N //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Song, J., Two consecutive zinc fingers in Sp1 and in MAZ are essential for interactions with cis-elements. J. Biol. Chem., 276, 30429-30434 (2001). PMID 11395515.
user report	Cogoi, S., MAZ-binding G4-decoy with locked nucleic acid and twisted intercalating nucleic acid modifications suppresses KRAS in pancreatic cancer cells and delays tumor growth in mice. Nucleic Acids Res. 2013 Apr;41(7):4049-64. PMID 23471001.
user report	Membrino, A., G4-DNA Formation in the HRAS Promoter and Rational Design of Decoy Oligonucleotides for Cancer Therapy. PLoS One. 6 (9): e24421 (2011). PMID 21931711.
user report	Cogoi S., The KRAS promoter responds to Myc-associated zinc finger and poly(ADP-ribose) polymerase 1 proteins, which recognize a critical quadruplex-forming GA-element. J. Biol. Chem., 285, 22003-22016 (2010). PMID 20457603.
user report	Wang H.Y., Transcriptional regulation of human eosinophil RNases by an evolutionary- conserved sequence motif in primate genome. BMC Mol. Biol., 8:89 (2007). PMID 17927842.

2021.06.03

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