RDB05635 - pmutant RAR RE-TK hRluc(F)

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pmutant RAR RE-TK hRluc(F) (#RDB05635)

Reporter construct of RAR response element, mutated

Alternative name	2490C2F
Clone info.	mutant RAR response element (RARE) is cloned into the BglII site of phRL-TK, and the BglII site is killed by the cloning. The nucleotide sequence of the mutant RARE is 5'-gatccagcacaaacatcag-3'. (Ref: J Biol Chem. 269: 890-902, 1994)
Comment	The nucleotide sequence of the element was determined. Function of the inserted element has not been examined by the depositor.
Vector backbone	phRL-TK (Plasmid)
Size of vector backbone	4.0 kb
Selectable markers	Amp^r
Gene/insert name	Artificial RAR response element, mutated synthesized
Depositor\|Developer	Yokoyama, Kazunari \|

Distribution information

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Terms and conditions for distribution The RECIPIENT agrees to expressly describe the acknowledgement of the Gene Engineering Division, RIKEN BRC as the source of the BIOLOGICAL RESOURCE in any publication. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for-profit organization for a not-for-profit research.
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The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.

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Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	The RECIPIENT agrees to expressly describe the acknowledgement of the Gene Engineering Division, RIKEN BRC as the source of the BIOLOGICAL RESOURCE in any publication. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for-profit organization for a not-for-profit research. Additional terms and conditions: The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件	リソースを使用して論文を投稿する場合、理研BRC・遺伝子材料開発室から分譲された事を記載する。本件リソースは非営利機関にのみ提供し、非営利研究にのみ利用することができる。 MTAに書く付加的使用条件：本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。

必要書類

提供依頼書

[依頼書の記入例

]
提供同意書 (MTA, 非営利学術目的用)[Word

] [MTAの記入例

]
手続きの概要は、「提供申込みについて[link]」をご覧ください。

MTAに書く使用条件

リソースを使用して論文を投稿する場合、理研BRC・遺伝子材料開発室から分譲された事を記載する。本件リソースは非営利機関にのみ提供し、非営利研究にのみ利用することができる。
MTAに書く付加的使用条件：
本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB05635	pmutant RAR RE-TK hRluc(F)	DNA solution

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pmutant RAR RE-TK hRluc(F) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB05635).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB12862_A4It.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: SV40pA3'Element Reverse Sequence file: RDB12862_A4Ita.seq
>05635_12862_A4It_SV40pA3'ElementReverse_C03_09.ab1 1 GAGGGTAGGG GGCGGAGCTA TGGAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC 61 CTTTTGCTGG CCTTTTGCTC ACATGGCTCG ACAGATCCAG CACAAACATC AGGATCTAAA 121 TGAGTCTTCG GACCTCGCGG GGGCCGCTTA AGCGGTGGTT AGGGTTTGTC TGACGCGGGG 181 GGAGGGGGAA GGAACGAAAC ACTCTCATTC GGAGGCGGCT CGGGGTTTGG TCTTGGTGGC 241 CACGGGCACG CAGAAGAGCG CCGCGATCCT CTTAAGCACC CCCCCGCCCT CCGTGGAGGC 301 GGGGGTTTGG TCGGCGGGTG GTAACTGGCG GGCCGCTGAC TCGGGCGGGT CGCGCGCCCC 361 AGAGTGTGAC CTTTTCGGTC TGCTCGCAGA CCCCCGGGCG GCGCCGCCGC GGCGGCGACG 421 GGCTCGCTGG GTCCTAGGCT CCATGGGGAC CGTATACGTG GACAGGCTCT GGAGCATCCG 481 CACGACTGCG GTGATATTAC CGGAGACCTT CTGCGGGACG AGCCGGGTCA CGCGGCTGAC 541 GCGGAGCGTC CGTTGGGCGA CAAACACCAG GACGGGGCAC AGGTACACTA TCTTGTCACC 601 CGGAGGCGCG AGGGACTGCA GGAGCTTCAG GGAGTGGCGC AGCTGCTTCA TCCCCGTGGC 661 CCGTTGCTCG CGTTTGCTGG CGGTGTCCCC GGAAGAAATA TATTTGCATG TCTTTAGTTC 721 TATGATGACA CAAACCCCGC CCAGCGTCTT GTCATTGGCG AATTCGAACA CGCAGATGCA 781 GTCGGGGCGG CGCGGTCCCA GGTCCACTTC GCATATTAAG GTGACGCGTG TGGCCTCGAA 841 CACCGAGCGA CCCTGCAGCG ACCCGCTTAA AAGCTTGATT CTTCTGACAC AACAGTCTCG 901 AACTTAAGCT GCAGAAGTTG GTCGTGAGGC ACTGGGCAGG TAAGTATCAA GGGTTACAAG 961 ACAGGGTTTA AAGGAGACCA ATAGAAACTG GGCTTGTCGA GACAGAGAAG ACTCTTGCGT 1021 TTTCTGATAG GCACCCTATT GGTCTTACTG ACATCCACTT TGCCCTTTTC TTCTCCACAG 1081 TGTCCACCTC CCAGATTCAT TACAGGCTCT AAGCTAGAGT ACTTATTACG ACTCACACTA 1141 ATATAGGG //

Primer: SV40pA3'Element Reverse
Sequence file: RDB12862_A4Ita.seq check

>05635_12862_A4It_SV40pA3'ElementReverse_C03_09.ab1
    1 GAGGGTAGGG GGCGGAGCTA TGGAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC
   61 CTTTTGCTGG CCTTTTGCTC ACATGGCTCG ACAGATCCAG CACAAACATC AGGATCTAAA
  121 TGAGTCTTCG GACCTCGCGG GGGCCGCTTA AGCGGTGGTT AGGGTTTGTC TGACGCGGGG
  181 GGAGGGGGAA GGAACGAAAC ACTCTCATTC GGAGGCGGCT CGGGGTTTGG TCTTGGTGGC
  241 CACGGGCACG CAGAAGAGCG CCGCGATCCT CTTAAGCACC CCCCCGCCCT CCGTGGAGGC
  301 GGGGGTTTGG TCGGCGGGTG GTAACTGGCG GGCCGCTGAC TCGGGCGGGT CGCGCGCCCC
  361 AGAGTGTGAC CTTTTCGGTC TGCTCGCAGA CCCCCGGGCG GCGCCGCCGC GGCGGCGACG
  421 GGCTCGCTGG GTCCTAGGCT CCATGGGGAC CGTATACGTG GACAGGCTCT GGAGCATCCG
  481 CACGACTGCG GTGATATTAC CGGAGACCTT CTGCGGGACG AGCCGGGTCA CGCGGCTGAC
  541 GCGGAGCGTC CGTTGGGCGA CAAACACCAG GACGGGGCAC AGGTACACTA TCTTGTCACC
  601 CGGAGGCGCG AGGGACTGCA GGAGCTTCAG GGAGTGGCGC AGCTGCTTCA TCCCCGTGGC
  661 CCGTTGCTCG CGTTTGCTGG CGGTGTCCCC GGAAGAAATA TATTTGCATG TCTTTAGTTC
  721 TATGATGACA CAAACCCCGC CCAGCGTCTT GTCATTGGCG AATTCGAACA CGCAGATGCA
  781 GTCGGGGCGG CGCGGTCCCA GGTCCACTTC GCATATTAAG GTGACGCGTG TGGCCTCGAA
  841 CACCGAGCGA CCCTGCAGCG ACCCGCTTAA AAGCTTGATT CTTCTGACAC AACAGTCTCG
  901 AACTTAAGCT GCAGAAGTTG GTCGTGAGGC ACTGGGCAGG TAAGTATCAA GGGTTACAAG
  961 ACAGGGTTTA AAGGAGACCA ATAGAAACTG GGCTTGTCGA GACAGAGAAG ACTCTTGCGT
1021 TTTCTGATAG GCACCCTATT GGTCTTACTG ACATCCACTT TGCCCTTTTC TTCTCCACAG
1081 TGTCCACCTC CCAGATTCAT TACAGGCTCT AAGCTAGAGT ACTTATTACG ACTCACACTA
1141 ATATAGGG
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

Resource search

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Other resources in our bank

External Database
Artificial RAR response element, mutated

Reference sequence