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pmutant RAR RE-TK hRluc(F)

Reporter construct of RAR response element, mutated

Catalog number RDB05635
Resource name pmutant RAR RE-TK hRluc(F)
Alternative name 2490C2F
Clone info. mutant RAR response element (RARE) is cloned into the BglII site of phRL-TK, and the BglII site is killed by the cloning. The nucleotide sequence of the mutant RARE is 5'-gatccagcacaaacatcag-3'. (Ref: J Biol Chem. 269: 890-902, 1994)
Comment The nucleotide sequence of the element was determined. Function of the inserted element has not been examined by the depositor.
Vector backbone phRL-TK (Plasmid)
Size of vector backbone 4.0 kb
Selectable markers Amp^r
Gene/insert name Artificial RAR response element, mutated synthesized
Depositor|Developer Yokoyama, Kazunari |
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Artificial RAR response element, mutated

            Reference sequence
              
             

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions for distribution The RECIPIENT agrees to expressly describe the acknowledgement of the Gene Engineering Division, RIKEN BRC as the source of the BIOLOGICAL RESOURCE in any publication. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for-profit organization for a not-for-profit research.
            Additional terms and conditions:
            The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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            RDB05635 pmutant RAR RE-TK hRluc(F) DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            How to cite this biological resource

            Materials & Methods section:

            The pmutant RAR RE-TK hRluc(F) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB05635).

            Reference section:

            Further references such as user reports and related articles (go to bottom)


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            QC test results

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB12862_A4It.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: SV40pA3'Element Reverse
            Sequence file: RDB12862_A4Ita.seq check
            >05635_12862_A4It_SV40pA3'ElementReverse_C03_09.ab1
                1 GAGGGTAGGG GGCGGAGCTA TGGAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC
               61 CTTTTGCTGG CCTTTTGCTC ACATGGCTCG ACAGATCCAG CACAAACATC AGGATCTAAA
              121 TGAGTCTTCG GACCTCGCGG GGGCCGCTTA AGCGGTGGTT AGGGTTTGTC TGACGCGGGG
              181 GGAGGGGGAA GGAACGAAAC ACTCTCATTC GGAGGCGGCT CGGGGTTTGG TCTTGGTGGC
              241 CACGGGCACG CAGAAGAGCG CCGCGATCCT CTTAAGCACC CCCCCGCCCT CCGTGGAGGC
              301 GGGGGTTTGG TCGGCGGGTG GTAACTGGCG GGCCGCTGAC TCGGGCGGGT CGCGCGCCCC
              361 AGAGTGTGAC CTTTTCGGTC TGCTCGCAGA CCCCCGGGCG GCGCCGCCGC GGCGGCGACG
              421 GGCTCGCTGG GTCCTAGGCT CCATGGGGAC CGTATACGTG GACAGGCTCT GGAGCATCCG
              481 CACGACTGCG GTGATATTAC CGGAGACCTT CTGCGGGACG AGCCGGGTCA CGCGGCTGAC
              541 GCGGAGCGTC CGTTGGGCGA CAAACACCAG GACGGGGCAC AGGTACACTA TCTTGTCACC
              601 CGGAGGCGCG AGGGACTGCA GGAGCTTCAG GGAGTGGCGC AGCTGCTTCA TCCCCGTGGC
              661 CCGTTGCTCG CGTTTGCTGG CGGTGTCCCC GGAAGAAATA TATTTGCATG TCTTTAGTTC
              721 TATGATGACA CAAACCCCGC CCAGCGTCTT GTCATTGGCG AATTCGAACA CGCAGATGCA
              781 GTCGGGGCGG CGCGGTCCCA GGTCCACTTC GCATATTAAG GTGACGCGTG TGGCCTCGAA
              841 CACCGAGCGA CCCTGCAGCG ACCCGCTTAA AAGCTTGATT CTTCTGACAC AACAGTCTCG
              901 AACTTAAGCT GCAGAAGTTG GTCGTGAGGC ACTGGGCAGG TAAGTATCAA GGGTTACAAG
              961 ACAGGGTTTA AAGGAGACCA ATAGAAACTG GGCTTGTCGA GACAGAGAAG ACTCTTGCGT
             1021 TTTCTGATAG GCACCCTATT GGTCTTACTG ACATCCACTT TGCCCTTTTC TTCTCCACAG
             1081 TGTCCACCTC CCAGATTCAT TACAGGCTCT AAGCTAGAGT ACTTATTACG ACTCACACTA
            1141 ATATAGGG
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles


            2023.11.08

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