Resource data sheet
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pRAR RE-TK hRluc(F) (#RDB05634)

Reporter construct of RAR response element

Alternative name 2489C2F
Clone info. RAR response element (RARE) is cloned into the BglII site of phRL-TK, and the BglII site is killed by the cloning. The nucleotide sequence of the RARE is 5'-gatccaggtcaaaggtcag-3'. (Ref: J Biol Chem. 269: 890-902, 1994)
Comment The nucleotide sequence of the element was determined. Function of the inserted element has not been examined by the depositor.
Vector backbone phRL-TK (Plasmid)
Size of vector backbone 4.0 kb
Selectable markers Amp^r
Gene/insert name Artificial RAR response element synthesized
Depositor|Developer Yokoyama, Kazunari |
 
Remarks, protocol and/or map (pdf) RDB05634.pdf

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Terms and conditions for distribution The RECIPIENT agrees to expressly describe the acknowledgement of the Gene Engineering Division, RIKEN BRC as the source of the BIOLOGICAL RESOURCE in any publication. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for-profit organization for a not-for-profit research.
Additional terms and conditions:
The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
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MTAに書く付加的使用条件：
本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB05634 pRAR RE-TK hRluc(F) DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pRAR RE-TK hRluc(F) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB05634).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB12845_A4Ib.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: SV40pA3'Element Reverse
Sequence file: RDB12845_A4Iba.seq check
>05634_12845_A4Ib_SV40pA3'element Reverse_B03_06.ab1
    1 GTCGCAGGAG GGCGGACCTA TGGAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC
   61 TTTTGCTGGC CTTTTGCTCA CATGGCTCGA CAGATCCAGG TCAAAGGTCA GGATCTAAAT
  121 GAGTCTTCGG ACCTCGCGGG GGCCGCTTAA GCGGTGGTTA GGGTTTGTCT GACGCGGGGG
  181 GAGGGGGAAG GAACGAAACA CTCTCATTCG GAGGCGGCTC GGGGTTTGGT CTTGGTGGCC
  241 ACGGGCACGC AGAAGAGCGC CGCGATCCTC TTAAGCACCC CCCCGCCCTC CGTGGAGGCG
  301 GGGGTTTGGT CGGCGGGTGG TAACTGGCGG GCCGCTGACT CGGGCGGGTC GCGCGCCCCA
  361 GAGTGTGACC TTTTCGGTCT GCTCGCAGAC CCCCGGGCGG CGCCGCCGCG GCGGCGACGG
  421 GCTCGCTGGG TCCTATGCTC CATGGGGTCC GTATACGTGG ACAGGCTCTG GAGCATCCGC
  481 ACGACTGCGG TGATATTACC GGAGACCTTC TGCGGGACGA GCCGGGTCAC GCGGCTGACG
541 CGGAGCGTCC GTTGGGCGAC AAACACCAGG ACGGGGCACA TGTACACTAT CTTGTC
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles