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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pKM2L-pvCMV

Promoter Bank clone, CMV cytomegalovirus immediate enhancer/promoter (CMV) promoter

Catalog number RDB05551
Resource name pKM2L-pvCMV
Alternative name p1971-6F
Clone info. The CMV cytomegalovirus immediate enhancer/promoter (CMV) promoter sequence (PCR-amplified ) was inserted into a Renilla luciferase reporter vector pKM2L (RDB04026).
Entire sequence of promoter region has not been confirmed.
Vector backbone pKM2L (RDB04026) (Plasmid)
Size of vector backbone 4.2 kb
Selectable markers Kan^r
Gene/insert name CMV cytomegalovirus immediate enhancer/promoter (CMV) genomic DNA
Depositor|Developer DNA Bank, |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR 1. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. 2. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Additional terms and conditions for distribution The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB05551 pKM2L-pvCMV DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Electronic file Sequence RDB05551z.seq

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Promoter Collection (Renilla Luciferase) (English text)
Featured content Control Vectors (English text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB12815_A4G3p1-2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: phRLR2primer
Region: Intron, CMV en_pro
Sequence file: RDB12815_A4G3d.seq check
>05551_12815_A4G3_phRLR2_E01_13.ab1
    1 AACTTCCGGT CTCGACAGCC CAGTTTCTAT TGGTCTCCTT AAACCTGTCT TGTAACCTTG
   61 ATACTTACCT GCCCAGTGCC TCACGACCAA CTTCTGCAGT ACCCGGGTGA ATTCTACGCG
  121 TCGACAGGCC ACTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA ATAAAGCTTC
  181 TAGTGATCTG ACGGTTCACT AAACGAGCTC TGCTTATATA GACCTCCCAC CGTACACGCC
  241 TACCGCCCAT TTGCGTCAAC GGGGCGGGGT TATTACGACA TTTTGGAAAG TCCCGTTGAT
  301 TTTGGTGCCA AAACAAACTC CCATTGACGT CAATGGGGTG GAGACTTGGA AATCCCCGTG
  361 AGTCAAACCG CTATCCACGC CCATTGGTGT ACTGCCAAAA CCGCATCACC ATGGTAATAG
  421 CGATGACTAA TACGTAGATG TACTGCCAAG TAGGAAAGTC CCGTAAGGTC ATGTACTGGG
  481 CATAATGCCA GGCGGGCCAT TTACCGTCAT TGACGTCAAT AGGGGGCGGA CTTGGCATAT
  541 GATACACTTG ATGTACTGCC AAGTGGGCAG TTTACCGTAA ATACTCCACC CATTGACGTC
  601 AATGGAAAGT CCCTATTGGC GTTACTATGG GAACATACGT CATTATTGAC GTCAATGGGC
  661 GGGGGTCGTT GGGCGGTCAG CCAGGCGGGC CATTTACCGT AAGTTATGTA ACGCGGAACT
  721 CCATATATGG GCTATGAACT AATGACCCCG TAATTGATTA CTATTAATAA CTAGTCAATA
  781 ATCAATGCCA ACATGGCGGT CATATTGGAC ATGAGCCAAT ATAAATGTAC ATATTATGAT
  841 ATAGATACAA CGTATGCAAT GGCCAATAGC CAATATTGAT TTATGCTATA TAACCAATGA
  901 ATAATATGGC TAATGGCCAA TATTGAGCCA CTAGTGCATG CATATGTGAA GCTTAGAGCT
  961 CGAGATCTTA ATCATTACCC TGTTTATCCC TATTTAAATG ACCTGCAGGC ATGCAAAGCT
 1021 TTGGCACTGG GCCGTCGTTT TTACACCGGT CGTTGACTGG TAAAACCCCT GACGTACCGA
1081 CTAGTTCGTC TTGCAGCACA TTCCCCCATT TTTCG
//
Primer: pAxCALNLF1
Region: hRluc
Sequence file: RDB12815_A4G3e.seq check
>05551_12815_A4G3_pAxCALNLF1_D01_10.ab1
    1 ATCAATTATC TTATCATGTC TGCTCGAAGC GGCCGCTCTA GAATTACTGC TCGTTCTTCA
   61 GCACGCGCTC CACGAAGCTC TTGATGTACT TACCCATTTC ATCTGGAGCG TCCTCCTGGC
  121 TGAAGTGGAG GCCCTTCACC TTCACGAACT CGGTGTTAGG GAACTTCTTA GCTCCCTCGA
  181 CAATAGCGTT GGAAAAGAAC CCAGGGTCGG ACTCGATGAA CATCTTAGGC AGATCGTCGC
  241 TGGCCCGAAG GTAGGCGTTG TAGTTGCGGA CAATCTGGAC GACGTCGGGC TTGCCTCCCT
  301 TAACGAGAGG GATCTCGCGA GGCCAGGAGA GGGTAGGCCG TCTAACCTCG CCCTTCTCCT
  361 TGAATGGCTC CAGGTAGGCA GCGAACTCCT CAGGCTCCAG TTTCCGCATG ATCTTGCTTG
  421 GGAGCATGGT CTCGACGAAG AAGTTATTCT CAAGCACCAT TTTCTCGCCC TCTTCGCTCT
  481 TGATCAGGGC GATATCCTCC TCGATGTCAG GCCACTCGTC CCAGGACTCG ATCACGTCCA
  541 CGACACTCTC AGCATGGACG ATGGCCTTGA TCTTGTCTTG GTGCTCGTAG GAGTAGTGAA
  601 AGGCCAGACA AGCCCCCCAG TCGTGGCCCA CAAAGATGAT TTTCTTTGGA AGGTTCAGCA
  661 GCTCGAACCA AGCGGTGAGG TACTTGTAGT GATCCAGGAG GCGATATGAG CCATTCCCGC
  721 TCTTGCCGGA CTTACCCATT CCGATCAGAT CAGGGATGAT GCATCTAGCC ACGGGCTCGA
  781 TGTGAGGCAC GACGTGCCTC CACAGGTAGC TGGAGGCAGC GTTACCATGC AGAAAAATCA
  841 CGGCGTTCTC GGCGTGCTTC TCGGAATCAT AGTAGTTGAT GAACGAAGTC CAGCACGTTC
  901 ATTTGCTTGC AGCGAGCCCA CCACTGAGGC CCAGTGATCA TGCGTTTGCG TTGCTCGGGG
  961 TCGTACACCT TTGGAAAGCC ATGGTGGGCT AGCCCTATAG TGAGTCGTTA TTAAGTAACT
 1021 TCTAGCCTTT AGAGCTGTAA TTGAACTTGG GAGTGAACAC CTGTGAAGAG AAAGGCTAAG
1081 TGGATGTCAG TAAGAACCAT AGGGTGCCCA TTATTCCAG
//
Primer: M13_-40
Region: CMV en_pro, intron
Sequence file: RDB12815_A4G3f.seq check
>05551_12815_A4G3_M13_(-40)_C01_07.ab1
    1 CCGGCCGGCC AGCTTGCATG CCTGCAGGTC ATTTAAATAG GGATAACAGG GTAATGATTA
   61 AGATCTCGAG CTCTAAGCTT CACATATGCA TGCACTAGTG GCTCAATATT GGCCATTAGC
  121 CATATTATTC ATTGGTTATA TAGCATAAAT CAATATTGGC TATTGGCCAT TGCATACGTT
  181 GTATCTATAT CATAATATGT ACATTTATAT TGGCTCATGT CCAATATGAC CGCCATGTTG
  241 GCATTGATTA TTGACTAGTT ATTAATAGTA ATCAATTACG GGGTCATTAG TTCATAGCCC
  301 ATATATGGAG TTCCGCGTTA CATAACTTAC GGTAAATGGC CCGCCTGGCT GACCGCCCAA
  361 CGACCCCCGC CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC CAATAGGGAC
  421 TTTCCATTGA CGTCAATGGG TGGAGTATTT ACGGTAAACT GCCCACTTGG CAGTACATCA
  481 AGTGTATCAT ATGCCAAGTC CGCCCCCTAT TGACGTCAAT GACGGTAAAT GGCCCGCCTG
  541 GCATTATGCC CAGTACATGA CCTTACGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT
  601 AGTCATCGCT ATTACCATGG TGATGCGGTT TTGGCAGTAC ACCAATGGGC GTGGATAGCG
  661 GTTTGACTCA CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG
  721 GCACCAAAAT CAACGGGACT TTCCAAAATG TCGTAATAAC CCCGCCCCGT TGACGCAAAT
  781 GGGCGGTAGG CGTGTACGGT GGGAGGTCTA TATAAGCAGA GCTCGTTTAG TGAACCGTCA
  841 GATCACTAGA AGCTTTATTG CGGTAGTTTA TCACAGTTAA ATTGCTAACG CAGTCAGTGG
  901 CCTGTCGACG CGTAGAATTC ACCCGGGTAC TGCAGAAGTT GGTCGTGAGG CACTGGGCAG
  961 GTAAGTATCA AAGGTTACAA GACAGGTTTA AAGGAGACCC AATAGAAACT GGGCTTTGTC
 1021 GAGACAGAGA GACTCTTTGC GTTTCTGATA GGCACCTATT GGATCCTAAC TGGACATTCA
1081 ACTTTGGCCG TTTCTTCTCC AACAAGGGGG TGTTGCA
//
Primer: CSII_CMV5_v2
Region: CMV en_pro, intron, hRluc
Sequence file: RDB12815_A4G3g.seq check
>05551_12815_B0AfF_CSII-CMV_5v2_H05_08_ABI08.ab1
    1 NNNNNNNNNN NNNNNNGTTN NTCNCNGTTA AATTGCTAAC GCAGTCNGTG GCCTGTCGAC
   61 GCGTAGAATT CACCCGGGTA CTGCAGAAGT TGGTCGTGAG GCACTGGGCA GGTAAGTATC
  121 AAGGTTACAA GACAGGTTTA AGGAGACCAA TAGAAACTGG GCTTGTCGAG ACAGAGAAGA
  181 CTCTTGCGTT TCTGATAGGC ACCTATTGGT CTTACTGACA TCCACTTTGC CTTTCTCTCC
  241 ACAGGTGTCC ACTCCCAGTT CAATTACAGC TCTTAAGGCT AGAGTACTTA ATACGACTCA
  301 CTATAGGCTA GCCACCATGG CTTCCAAGGT GTACGACCCC GAGCAACGCA AACGCATGAT
  361 CACTGGGCCT CAGTGGTGGG CTCGCTGCAA GCAAATGAAC GTGCTGGACT CCTTCATCAA
  421 CTACTATGAT TCCGAGAAGC ACGCCGAGAA CGCCGTGATT TTTCTGCATG GTAACGCTGC
  481 CTCCAGCTAC CTGTGGAGGC ACGTCGTGCC TCACATCGAG CCCGTGGCTA GATGCATCAT
  541 CCCTGATCTG ATCGGAATGG GTAAGTCCGG CAAGAGCGGG AATGGCTCAT ATCGCCTCCT
  601 GGATCACTAC AAGTACCTCA CCGCTTGGTT CGAGCTGCTG AACCTTCCAA AGAAAATCAT
  661 CTTTGTGGGC CACGACTGGG GGGCTTGTCT GGCCTTTCAC TACTCCTACG AGCACCAAGA
  721 CAAGATCAAG GCCATCGTCC ATGCTGAGAG TGTCGTGGAC GTGATCGAGT CCTGGGANGA
  781 GTGGNCTGAC ATCGAGGANG ANATCGCCCT GATCNAGANC GAANANGGCG ANAAAATGGT
  841 GCTTGANAAT AACTTCTTCN TCNANANCAT GCTCCCNANC AAGATCATGC GGAAANNGGA
  901 NNNNGANGAN TTCNCTGNCN ANCNGNNNCC NTTCNNNGAN ANNNNNGNNN TNANANNGNC
961 NANCNNNNNN NNNN
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

user report Kakimoto, M., Spermine synthesis inhibitor blocks 25-hydroxycholesterol-induced- apoptosis via SREBP2 upregulation in DLD-1 cell spheroids. Biochem .Biophys. Rep. 22: 100754 (2020). PMID 32258442.

2020.10.02

GNP_filter3_RDBDEP_html_200316.pl