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pKM2L-pvCMV

Promoter Bank clone, CMV cytomegalovirus immediate enhancer/promoter (CMV) promoter

Catalog number RDB05551
Resource name pKM2L-pvCMV
Alternative name p1971-6F
Clone info. The CMV cytomegalovirus immediate enhancer/promoter (CMV) promoter sequence (PCR-amplified ) was inserted into a Renilla luciferase reporter vector pKM2L (RDB04026).
Comment Entire sequence of promoter region has not been confirmed.
Vector backbone pKM2L (RDB04026) (Plasmid)
Size of vector backbone 4.2 kb
Selectable markers Kan^r
Gene/insert name CMV cytomegalovirus immediate enhancer/promoter (CMV) genomic DNA
Depositor|Developer DNA Bank, |
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External Database
CMV cytomegalovirus immediate enhancer/promoter (CMV)

            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR 1. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. 2. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
            Additional terms and conditions for distribution The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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            RDB05551 pKM2L-pvCMV DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Sequence RDB05551z.seq

            Original reference

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Promoter Collection (Renilla Luciferase) (English text)
            Featured content Control Vectors (English text)

            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB12815_A4G3p1-2.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: phRLR2primer
            Region: Intron, CMV en_pro
            Sequence file: RDB12815_A4G3d.seq check
            >05551_12815_A4G3_phRLR2_E01_13.ab1
                1 AACTTCCGGT CTCGACAGCC CAGTTTCTAT TGGTCTCCTT AAACCTGTCT TGTAACCTTG
               61 ATACTTACCT GCCCAGTGCC TCACGACCAA CTTCTGCAGT ACCCGGGTGA ATTCTACGCG
              121 TCGACAGGCC ACTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA ATAAAGCTTC
              181 TAGTGATCTG ACGGTTCACT AAACGAGCTC TGCTTATATA GACCTCCCAC CGTACACGCC
              241 TACCGCCCAT TTGCGTCAAC GGGGCGGGGT TATTACGACA TTTTGGAAAG TCCCGTTGAT
              301 TTTGGTGCCA AAACAAACTC CCATTGACGT CAATGGGGTG GAGACTTGGA AATCCCCGTG
              361 AGTCAAACCG CTATCCACGC CCATTGGTGT ACTGCCAAAA CCGCATCACC ATGGTAATAG
              421 CGATGACTAA TACGTAGATG TACTGCCAAG TAGGAAAGTC CCGTAAGGTC ATGTACTGGG
              481 CATAATGCCA GGCGGGCCAT TTACCGTCAT TGACGTCAAT AGGGGGCGGA CTTGGCATAT
              541 GATACACTTG ATGTACTGCC AAGTGGGCAG TTTACCGTAA ATACTCCACC CATTGACGTC
              601 AATGGAAAGT CCCTATTGGC GTTACTATGG GAACATACGT CATTATTGAC GTCAATGGGC
              661 GGGGGTCGTT GGGCGGTCAG CCAGGCGGGC CATTTACCGT AAGTTATGTA ACGCGGAACT
              721 CCATATATGG GCTATGAACT AATGACCCCG TAATTGATTA CTATTAATAA CTAGTCAATA
              781 ATCAATGCCA ACATGGCGGT CATATTGGAC ATGAGCCAAT ATAAATGTAC ATATTATGAT
              841 ATAGATACAA CGTATGCAAT GGCCAATAGC CAATATTGAT TTATGCTATA TAACCAATGA
              901 ATAATATGGC TAATGGCCAA TATTGAGCCA CTAGTGCATG CATATGTGAA GCTTAGAGCT
              961 CGAGATCTTA ATCATTACCC TGTTTATCCC TATTTAAATG ACCTGCAGGC ATGCAAAGCT
             1021 TTGGCACTGG GCCGTCGTTT TTACACCGGT CGTTGACTGG TAAAACCCCT GACGTACCGA
            1081 CTAGTTCGTC TTGCAGCACA TTCCCCCATT TTTCG
            //
            Primer: pAxCALNLF1
            Region: hRluc
            Sequence file: RDB12815_A4G3e.seq check
            >05551_12815_A4G3_pAxCALNLF1_D01_10.ab1
                1 ATCAATTATC TTATCATGTC TGCTCGAAGC GGCCGCTCTA GAATTACTGC TCGTTCTTCA
               61 GCACGCGCTC CACGAAGCTC TTGATGTACT TACCCATTTC ATCTGGAGCG TCCTCCTGGC
              121 TGAAGTGGAG GCCCTTCACC TTCACGAACT CGGTGTTAGG GAACTTCTTA GCTCCCTCGA
              181 CAATAGCGTT GGAAAAGAAC CCAGGGTCGG ACTCGATGAA CATCTTAGGC AGATCGTCGC
              241 TGGCCCGAAG GTAGGCGTTG TAGTTGCGGA CAATCTGGAC GACGTCGGGC TTGCCTCCCT
              301 TAACGAGAGG GATCTCGCGA GGCCAGGAGA GGGTAGGCCG TCTAACCTCG CCCTTCTCCT
              361 TGAATGGCTC CAGGTAGGCA GCGAACTCCT CAGGCTCCAG TTTCCGCATG ATCTTGCTTG
              421 GGAGCATGGT CTCGACGAAG AAGTTATTCT CAAGCACCAT TTTCTCGCCC TCTTCGCTCT
              481 TGATCAGGGC GATATCCTCC TCGATGTCAG GCCACTCGTC CCAGGACTCG ATCACGTCCA
              541 CGACACTCTC AGCATGGACG ATGGCCTTGA TCTTGTCTTG GTGCTCGTAG GAGTAGTGAA
              601 AGGCCAGACA AGCCCCCCAG TCGTGGCCCA CAAAGATGAT TTTCTTTGGA AGGTTCAGCA
              661 GCTCGAACCA AGCGGTGAGG TACTTGTAGT GATCCAGGAG GCGATATGAG CCATTCCCGC
              721 TCTTGCCGGA CTTACCCATT CCGATCAGAT CAGGGATGAT GCATCTAGCC ACGGGCTCGA
              781 TGTGAGGCAC GACGTGCCTC CACAGGTAGC TGGAGGCAGC GTTACCATGC AGAAAAATCA
              841 CGGCGTTCTC GGCGTGCTTC TCGGAATCAT AGTAGTTGAT GAACGAAGTC CAGCACGTTC
              901 ATTTGCTTGC AGCGAGCCCA CCACTGAGGC CCAGTGATCA TGCGTTTGCG TTGCTCGGGG
              961 TCGTACACCT TTGGAAAGCC ATGGTGGGCT AGCCCTATAG TGAGTCGTTA TTAAGTAACT
             1021 TCTAGCCTTT AGAGCTGTAA TTGAACTTGG GAGTGAACAC CTGTGAAGAG AAAGGCTAAG
            1081 TGGATGTCAG TAAGAACCAT AGGGTGCCCA TTATTCCAG
            //
            Primer: M13_-40
            Region: CMV en_pro, intron
            Sequence file: RDB12815_A4G3f.seq check
            >05551_12815_A4G3_M13_(-40)_C01_07.ab1
                1 CCGGCCGGCC AGCTTGCATG CCTGCAGGTC ATTTAAATAG GGATAACAGG GTAATGATTA
               61 AGATCTCGAG CTCTAAGCTT CACATATGCA TGCACTAGTG GCTCAATATT GGCCATTAGC
              121 CATATTATTC ATTGGTTATA TAGCATAAAT CAATATTGGC TATTGGCCAT TGCATACGTT
              181 GTATCTATAT CATAATATGT ACATTTATAT TGGCTCATGT CCAATATGAC CGCCATGTTG
              241 GCATTGATTA TTGACTAGTT ATTAATAGTA ATCAATTACG GGGTCATTAG TTCATAGCCC
              301 ATATATGGAG TTCCGCGTTA CATAACTTAC GGTAAATGGC CCGCCTGGCT GACCGCCCAA
              361 CGACCCCCGC CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC CAATAGGGAC
              421 TTTCCATTGA CGTCAATGGG TGGAGTATTT ACGGTAAACT GCCCACTTGG CAGTACATCA
              481 AGTGTATCAT ATGCCAAGTC CGCCCCCTAT TGACGTCAAT GACGGTAAAT GGCCCGCCTG
              541 GCATTATGCC CAGTACATGA CCTTACGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT
              601 AGTCATCGCT ATTACCATGG TGATGCGGTT TTGGCAGTAC ACCAATGGGC GTGGATAGCG
              661 GTTTGACTCA CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG
              721 GCACCAAAAT CAACGGGACT TTCCAAAATG TCGTAATAAC CCCGCCCCGT TGACGCAAAT
              781 GGGCGGTAGG CGTGTACGGT GGGAGGTCTA TATAAGCAGA GCTCGTTTAG TGAACCGTCA
              841 GATCACTAGA AGCTTTATTG CGGTAGTTTA TCACAGTTAA ATTGCTAACG CAGTCAGTGG
              901 CCTGTCGACG CGTAGAATTC ACCCGGGTAC TGCAGAAGTT GGTCGTGAGG CACTGGGCAG
              961 GTAAGTATCA AAGGTTACAA GACAGGTTTA AAGGAGACCC AATAGAAACT GGGCTTTGTC
             1021 GAGACAGAGA GACTCTTTGC GTTTCTGATA GGCACCTATT GGATCCTAAC TGGACATTCA
            1081 ACTTTGGCCG TTTCTTCTCC AACAAGGGGG TGTTGCA
            //
            Primer: CSII_CMV5_v2
            Region: CMV en_pro, intron, hRluc
            Sequence file: RDB12815_A4G3g.seq check
            >05551_12815_B0AfF_CSII-CMV_5v2_H05_08_ABI08.ab1
                1 NNNNNNNNNN NNNNNNGTTN NTCNCNGTTA AATTGCTAAC GCAGTCNGTG GCCTGTCGAC
               61 GCGTAGAATT CACCCGGGTA CTGCAGAAGT TGGTCGTGAG GCACTGGGCA GGTAAGTATC
              121 AAGGTTACAA GACAGGTTTA AGGAGACCAA TAGAAACTGG GCTTGTCGAG ACAGAGAAGA
              181 CTCTTGCGTT TCTGATAGGC ACCTATTGGT CTTACTGACA TCCACTTTGC CTTTCTCTCC
              241 ACAGGTGTCC ACTCCCAGTT CAATTACAGC TCTTAAGGCT AGAGTACTTA ATACGACTCA
              301 CTATAGGCTA GCCACCATGG CTTCCAAGGT GTACGACCCC GAGCAACGCA AACGCATGAT
              361 CACTGGGCCT CAGTGGTGGG CTCGCTGCAA GCAAATGAAC GTGCTGGACT CCTTCATCAA
              421 CTACTATGAT TCCGAGAAGC ACGCCGAGAA CGCCGTGATT TTTCTGCATG GTAACGCTGC
              481 CTCCAGCTAC CTGTGGAGGC ACGTCGTGCC TCACATCGAG CCCGTGGCTA GATGCATCAT
              541 CCCTGATCTG ATCGGAATGG GTAAGTCCGG CAAGAGCGGG AATGGCTCAT ATCGCCTCCT
              601 GGATCACTAC AAGTACCTCA CCGCTTGGTT CGAGCTGCTG AACCTTCCAA AGAAAATCAT
              661 CTTTGTGGGC CACGACTGGG GGGCTTGTCT GGCCTTTCAC TACTCCTACG AGCACCAAGA
              721 CAAGATCAAG GCCATCGTCC ATGCTGAGAG TGTCGTGGAC GTGATCGAGT CCTGGGANGA
              781 GTGGNCTGAC ATCGAGGANG ANATCGCCCT GATCNAGANC GAANANGGCG ANAAAATGGT
              841 GCTTGANAAT AACTTCTTCN TCNANANCAT GCTCCCNANC AAGATCATGC GGAAANNGGA
              901 NNNNGANGAN TTCNCTGNCN ANCNGNNNCC NTTCNNNGAN ANNNNNGNNN TNANANNGNC
            961 NANCNNNNNN NNNN
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            user_report Kakimoto, M., Spermine synthesis inhibitor blocks 25-hydroxycholesterol-induced- apoptosis via SREBP2 upregulation in DLD-1 cell spheroids. Biochem .Biophys. Rep. 22: 100754 (2020). PMID 32258442.

            2022.05.18

            GNP_filter3_RDBDEP_html_220516.pl