RDB05549 - pKM2L-pvTK

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pKM2L-pvTK (#RDB05549)

Promoter Bank clone, HSV herpes simplex virus thymidine kinase (HSV-TK) promoter

Alternative name	p1969-4F
Clone info.	The HSV herpes simplex virus thymidine kinase (HSV-TK) promoter sequence (PCR-amplified ) was inserted into a Renilla luciferase reporter vector pKM2L (RDB04026).
Comment	Entire sequence of promoter region has not been confirmed.
Vector backbone	pKM2L (RDB04026) (Plasmid)
Size of vector backbone	4.2 kb
Selectable markers	Kan^r
Gene/insert name	HSV herpes simplex virus thymidine kinase (HSV-TK) genomic DNA
Depositor\|Developer	DNA Bank, \|

Sequence	RDB05549z.seq

Distribution information

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Terms and conditions for distribution 1. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. 2. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Additional terms and conditions:
The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.

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Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	1. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. 2. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. Additional terms and conditions: The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件	1. 本件リソースは学術研究機関にのみ提供し、学術研究にのみ利用することができる。　2. 謝辞の表明を必要とする。 MTAに書く付加的使用条件：本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB05549	pKM2L-pvTK	DNA solution

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pKM2L-pvTK was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB05549).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB19664_B2Gdp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pBAC_lac_F (Pr0544) Region: HSV TK pro 5' Sequence file: RDB19664_B2Gda.seq
>05549_19664_B2Gd_pBAC_lac_F_A06_03_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NAGCTTGCNT GCCTGCNGGT CATTTAAATA GGGATAACAG 61 GGTAATGATT AANATCNCNA GCTCTAAGCT TCACATATGC ATGCACTAGT GGCAAATGAG 121 TCTTCGGACC TCGCGGGGGC CGCTTAAGCG GTGGTTAGGG TTTGTCTGAC GCGGGGGGAG 181 GGGGAAGGAA CGAAACACTC TCATTCGGAG GCGGCTCGGG GTTTGGTCTT GGTGGCCACG 241 GGCACGCAGA AGAGCGCCGC GATCCTCTTA AGCACCCCCC CGCCCTCCGT GGAGGCGGGG 301 GTTTGGTCGG CGGGTGGTAA CTGGCGGGCC GCTGACTCGG GCGGGTCGCG CGCCCCAGAG 361 TGTGACCTTT TCGGTCTGCT CGCAGACCCC CGGGCGGCGC CGCCGCGGCG GCGACGGGCT 421 CGCTGGGTCC TAGGCTCCAT GGGGACCGTA TACGTGGACA GGCTCTGGAG CATCCGCACG 481 ACTGCGGTGA TATTACCGGA GACCTTCTGC GGGACGAGCC GGGTCACGCG GCTGACGCGG 541 AGCGTCCGTT GGGCGACAAA CACCAGGACG GGGCACAGGT ACACTATCTT GTCACCCGGA 601 GGCGCGAGGG ACTGCAGGAG CTTCAGGGAG TGGCGCAGCT GCTTCATCCC CGTGGCCCGT 661 TGCTCGCGTT TGCTGGCGGT GTCCCCGGAA GAAATATATT TGCATGTCTT TAGTTCTATG 721 ATGACACAAA CCCCGCCCAG CGTCTTGTCA TTGGCGAATT CGAACACGCA GATGCAGTCG 781 GGGCGGCGCG GTCCCAGGTC CACTTCGCAT ATTAAGGTGA CGCGTGTGGC CTCGAACACC 841 GANCGACCCT GCAGCGNNCC GCTTAAGCCT GTCGACGCGT ANAATTCANC CGGGTACTGC 901 ANAAGTTGGT CGTGAGGNAC TGGGCANGNA AGTATCAAGG TTACAAGANA GGTTTNANNN 961 NANCNATAGA ANNTGGGCTT GNCNANNNAG ANAANANTCT NGCGTTTCNG ANNNGNACCN 1021 ATNNNNNNAN NNNNNTCCNN TTNNNNTTNN NNNNNNNN //
Primer: phRLR2primer (Pr0083) Region: chimeric intron,HSV TK pro 3' Sequence file: RDB19664_B2Gdb.seq
>05549_19664_B2Gd_phRLR2primer_B06_06_ABI24.ab1 1 NNNNNNNNNN NNNNNNNAGC CNNGTTTCTN TTGGTCTCCT TAAACCTGTC TTGTAACCTT 61 GATACTTACC TGCCCAGTGC CTCACGACCA ACTTCTGCAG TACCCGGGTG AATTCTACGC 121 GTCGACAGGC TTAAGCGGGT CGCTGCAGGG TCGCTCGGTG TTCGAGGCCA CACGCGTCAC 181 CTTAATATGC GAAGTGGACC TGGGACCGCG CCGCCCCGAC TGCATCTGCG TGTTCGAATT 241 CGCCAATGAC AAGACGCTGG GCGGGGTTTG TGTCATCATA GAACTAAAGA CATGCAAATA 301 TATTTCTTCC GGGGACACCG CCAGCAAACG CGAGCAACGG GCCACGGGGA TGAAGCAGCT 361 GCGCCACTCC CTGAAGCTCC TGCAGTCCCT CGCGCCTCCG GGTGACAAGA TAGTGTACCT 421 GTGCCCCGTC CTGGTGTTTG TCGCCCAACG GACGCTCCGC GTCAGCCGCG TGACCCGGCT 481 CGTCCCGCAG AAGGTCTCCG GTAATATCAC CGCAGTCGTG CGGATGCTCC AGAGCCTGTC 541 CACGTATACG GTCCCCATGG AGCCTAGGAC CCAGCGAGCC CGTCGCCGCC GCGGCGGCGC 601 CGCCCGGGGG TCTGCGAGCA GACCGAAAAG GTCACACTCT GGGGCGCGCG ACCCGCCCGA 661 GTCAGCGGCC CGCCAGTTAC CACCCGCCGA CCAAACCCCC GCCTCCACGG AGGGCGGGGG 721 GGTGCTTAAG AGGATCGCGG CGCTCTTCTG CGTGCCCGTG GCCACCAAGA CCAAACCCCG 781 AGCCGCCTCC GAATGAGAGT GTTTCGTTCC TTNCCCCTCN CCCCGCGTCA GANAAACCCT 841 AACCACCGCT TAAGCGGCCC CCGCGAGGTC CGAAGANTCN TTTGCCACTA GTGNATGCAT 901 ATGTGAAGCT TANAGCTCGA GATCTTAATC ANTANCCTGT TATNCCNATT TAAATGANCN 961 GNNNNNTGCN AGCTTGGNNN TGGNNGNCNT TTTNCNNCGN CNNGNNNNGN NNAANCNNNN 1021 NNNNNCCCNN NNTNNNNNNN NNTNNNNNCN NNNNCCCNNT NNNNNNNNNN //
Primer: chimeric intron 3-prime (Pr0444) Region: T7 pro,hRluc 5' Sequence file: RDB19664_B2Gdc.seq
>05549_19664_B2Gd_chimeric intron 3-prime_D02_04_ABI08.ab1 1 NNNNNNNNNN NNNNNNTNNN TTACNGCTCT TNNGGNTAGA GTACTTAATA CGACTCACTA 61 TAGGCTAGCC ACCATGGCTT CCAAGGTGTA CGACCCCGAG CAACGCAAAC GCATGATCAC 121 TGGGCCTCAG TGGTGGGCTC GCTGCAAGCA AATGAACGTG CTGGACTCCT TCATCAACTA 181 CTATGATTCC GAGAAGCACG CCGAGAACGC CGTGATTTTT CTGCATGGTA ACGCTGCCTC 241 CAGCTACCTG TGGAGGCACG TCGTGCCTCA CATCGAGCCC GTGGCTAGAT GCATCATCCC 301 TGATCTGATC GGAATGGGTA AGTCCGGCAA GAGCGGGAAT GGCTCATATC GCCTCCTGGA 361 TCACTACAAG TACCTCACCG CTTGGTTCGA GCTGCTGAAC CTTCCAAAGA AAATCATCTT 421 TGTGGGCCAC GACTGGGGGG CTTGTCTGGN CTTTCACTAC TCCTACGAGC ACCAAGACAA 481 GATCAAGGCC ATCGTCCATG CTGAGAGTGT CGTGNANNTG ATCGANTCCT GGGGACGAGT 541 GGCCTGACNT CNANNANGAN NTCGNCCTGN TNNNNANCGA ANANGGNGAN AAAATGNNGC 601 TTGNNAANNA CNTCTTCNNC NANNNNNTGC TCCNNNNNNN NATNNNGNNN AAACTNNNNN 661 NTGANNANNN NNNTNNNNAN CTNNNNNNNN NCNNGNNNNA NNGNNNNGNN NNANNNNNNN 721 NNN //
Primer: SV40pA_R (Pr0882) Region: SV40 pA,hRluc 3' Sequence file: RDB19664_B2Gdd.seq
>05549_19664_B2Gd_SV40pA_R_E02_05_ABI08.ab1 1 NNNNNNNNNN NNTCNNNNNN NTNNNNAATA AAGCATTTTT TTNNCTGCAT TCTAGTTGTG 61 GTTTGTCCAA NCTNNTCAAT GTATCTTATC ATGTCTGCTC GAAGCGGCCG CTCTAGAATT 121 ACTGCTCGTT CTTCAGCACG CGCTCCACGA AGCTCTTGAT GTACTTACCC ATTTCATCTG 181 GAGCGTCCTC CTGGCTGAAG TGGAGGCCCT TCACCTTCAC GAACTCGGTG TTAGGGAACT 241 TCTTAGCTCC CTCGACAATA GCGTTGGAAA AGAACCCAGG GTCGGACTCG ATGAACATCT 301 TAGGCAGATC GTCGCTGGCC CGAAGGTAGG CGTTGTAGTT GCGGACAATC TGGACGACGT 361 CGGGCTTGCC TCCCTTAACG AGAGGGATCT CGCGAGGCCA GGAGAGGGTA GGCCGTCTAA 421 CCTCGCCCTT CTCCTTGAAT GGCTCCAGGT AGGCAGCGAA CTCCTCAGGC TCCAGTTTCC 481 GCATGATCTT GCTTGGGAGC ATGGTCTCGA CGAAGAAGTT ATTCTCAAGC ACCATTTTCT 541 CGCCCTCTTC GCTCTTGATC AGGGCGATAT CCTCCTCGAT GTCAGGCCAC TCGTCCCAGG 601 ACTCGATCAC GTCCACGACA CTCTCAGCAT GGACGATGGC CTTGATCTTG TCTTGGTGCT 661 CGTAGGAGTA GTGAAAGGCC AGACAAGCCC CCCAGTCGTG GCCCACAAAG ATGATTTTCT 721 TTGNAAGGTT CAGCAGCTCG AACCAAGCGG TGAGGTACTT GTAGTGNTCC AGGANGCGAT 781 ATGAGCCATT CCCGCTCTTG CCGGACTTAC CCATTCCGAT CAGATCANGG ATGATGCATC 841 TAGCCACGGG CTCGATGTGA GGNACGACGN GCCTCCACAN GTAGCTGGAN GCAGCGTTAC 901 NNTGCANAAA AATCANGGCG TTCTCNGCGT GCNTCNCGGN ATCNTANTAN TTGNNGNANG 961 GANTNCNNNN NCGNNNATTT GCNTNGCNNC NAGCCNNNCN ACTGNNNNCN NNNNNNNNN //