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pKM2L-phHSP701 (#RDB05541)

Promoter Bank clone, Human 72-kDa heat shock protein (HSP70-1) promoter

Alternative name p1959-17F
Clone info. PCR amplified human 72-kDa heat shock protein (HSP70-1) promoter sequence was inserted into a Renilla luciferase reporter vector pKM2L (RDB04026). An HSP70-1 promoter -752 to +164 (+1 as the transcription start site. Fig.2 of Wu, B.J. Proc. Natl. Acad. Sci. USA 83, 629-633, 1986) corresponding to the nucleotide sequence from 1171 to 2086 of DQ451402.1 is cloned. Alternatively, 31814789 to 31815704(NC_000006.12); relatively -725 to +191, where +1 corresponds to 1 nt of NM_005345.6 was cloned.
Comment PCR cloning, forward primer, 1959 F2: 5' cctcaggcttaaaccaactagggaa 3'; reverse primer, 1959 R2: 5' gaacactggatccgcgagaagag 3' Entire sequence of promoter region has not been confirmed.
Vector backbone pKM2L (RDB04026) (Plasmid)
Size of vector backbone 4.2 kb
Selectable markers Kan^r
Gene/insert name Human 72-kDa heat shock protein (HSP70-1) genomic DNA
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RDB05541 pKM2L-phHSP701 DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pKM2L-phHSP701 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB05541).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB14249_A6Gep1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: M13_-40
Sequence file: RDB14249_A6Gea.seq check
>05541_14249_A6Ge_M13(-40)_A02_02.ab1
    1 CGCGGGTCAG CTTGCATGCC TGCAGGTCAT TTAAATAGGG ATAACAGGGT AATGATTAAG
   61 ATCTCGAGCT CTAAGCTTCA CATATGCATG CACTAGTGGC CCTCAGGCTT AAACCAACTA
  121 GGGAACTTTC CAGTACTTTC CCAAACAAGG ACCTACTGAG CCTTTCAGGT TCACAATCAA
  181 TCAGATCCCT ACTGGCTCAC CTAGTCTCCC GACGCCTTCG CTTCAGTTTG GAAACGTCCA
  241 GATTACGCAG CCCCAGCGAG TAGGTGGGGG CTCCCTCAAT ATCAAACTGC ACAACCGGGG
  301 TCCCCCCACC CCCCACCCCG TCCCTCCCTG CAAATTTGAG ACGGCTCCAA CTCAGTAATC
  361 TTTTTCCAAA CTGGCCCATG AGGTCAGAGA CAGTATCTCC ATTGTAACGT GGCCGGGCGG
  421 TGTCAACACA AACGCCCCCA CCCTCCCCTG GACGCGCGTA ACCCGCTCCC CGCACCAGCC
  481 CCCTGCCCAC AACTGCGCAG GCCCAGCAAG CCCCCACAAT TAAAAGCCCA GCGCCGACCC
  541 TTCCTGTCAA TTAGGCGCTG AAGCGCAGGC GGTCAGCATC GCCATGGAGA CCAACACCCT
  601 TCCCACCGCC ACTCCCCCTT CCTCTCAGGG TCCCTGTCCC CTCCAGTGAA TCCCAGAAGA
  661 CTCTGGAGAG TTCTGAGCAG GGGGCGGCAC TCTGGCCTCT GATTGGTCCA AGGAAGGCTG
  721 GGGGGCAGGA CGGGAGGCGA AAACCCTGGA ATATTCCCGA CCTGGCAGCC TCATCGAGCT
  781 CGGTGATTGG CTCAGAAGGG AAAAGGCGGG TCTCCGTGAC GACTTATAAA AGCCCAGGGG
  841 CAAGCGGTCC GGATAACGGC TAGCCTGAGG AGCTGCTGCG ACAGTCCACT ACCTTTTTTC
  901 GAGAGTGACT CCCGTTGTCC CAAAGGCTTC CCAGAGCGAA ACCTGTGCGG CTGCAGCACC
  961 GGGCGCGTCG AGTTTCCCGG CGTCCGGGAA AGGACCCGAG CTCTTCTCGC GGGATCCCAG
 1021 TGCTCGCCTG TCGACGCGTA GAACTCACCC GGGTTACTGC AGAAGTTGGA TCGTGAGGCA
1081 CTTGGGCAAG CTTAAGCTAT CAAGGGTTAA CCAG
//
Primer: phRLR2
Sequence file: RDB14249_A6Geb.seq check
>05541_14249_A6Ge_phRLR2_B02_05.ab1
    1 ATCCTATCTG ACAGCCCAGT TTCTATTGGT CTCCTTAAAC CTGTCTTGTA ACCTTGATAC
   61 TTACCTGCCC AGTGCCTCAC GACCAACTTC TGCAGTACCC GGGTGAATTC TACGCGTCGA
  121 CAGGCGAACA CTGGATCCGC GAGAAGAGCT CGGTCCTTCC GGACGCCGGA AACTCGACGC
  181 GCCGGTGCCT GCAGCCGCAC AGGTTCGCTC TGGGAAGCCT TGGGACAACG GGAGTCACTC
  241 TCGAAAAAGG TAGTGGACTG TCGCAGCAGC TCCTCAGGCT AGCCGTTATC CGGACCGCTT
  301 GCCCCTGGGC TTTTATAAGT CGTCACGGAG ACCCGCCTTT TCCCTTCTGA GCCAATCACC
  361 GAGCTCGATG AGGCTGCCAG GTCGGGAATA TTCCAGGGTT TTCGCCTCCC GTCCTGCCCC
  421 CCAGCCTTCC TTGGACCAAT CAGAGGCCAG AGTGCCGCCC CCTGCTCAGA ACTCTCCAGA
  481 GTCTTCTGGG ATTCACTGGA GGGGACAGGG ACCCTGAGAG GAAGGGGGAG TGGCGGTGGG
  541 AAGGGTGTTG GTCTCCATGG CGATGCTGAC CGCCTGCGCT TCAGCGCCTA ATTGACAGGA
  601 AGGGTCGGCG CTGGGCTTTT AATTGTGGGG GCTTGCTGGG CCTGCGCAGT TGTGGGCAGG
  661 GGGCTGGTGC GGGGAGCGGG TTACGCGCGT CCAGGGGAGG GTGGGGGCGT TTGTGTTGAC
  721 ACCGCCCGGC CACGTTACAA TGGAGATACT GTCTCTGACC TCATGGGCCA GTTTGGAAAA
  781 AGATTACTGA GTTGGAGCCG TCTCAAATTT GCAGGGAGGG ACGGGGTGGG GGGGGGTGGG
  841 GGGGGACCCC GGTTGTGCAG TTTTGATATT GGAGGGAGCC CCCCACCCTA CTCGCTGGGG
  901 GCTGCGTAAT CTGGGACGTT TCCAAACTGA AAGCGAAAGG CGTCGGGAGA CTAGGTGAGC
  961 CAGTAGGGAT CTGATTGATT GTGAACCCTG AAGCTCAGTA GTCCCTTGTT TGGGAAAGTA
 1021 CTGCAAAGTT CCCTAGTTTG GTTTAGGCCT GAAGGACCAA CTAGTGCATG CATATGTGAA
1081 AGGCTTAAGA GCTCGGAGAA TTCATTAATT CAA
//
Primer: pAxCALNL_F1
Sequence file: RDB14249_A6Gec.seq check
>05541_14249_A6Ge_pAxCALNL_F1_C02_08.ab1
    1 TCATTATCTT ATCATGTCTG CTCGAAGCGG CCGCTCTAGA ATTACTGCTC GTTCTTCAGC
   61 ACGCGCTCCA CGAAGCTCTT GATGTACTTA CCCATTTCAT CTGGAGCGTC CTCCTGGCTG
  121 AAGTGGAGGC CCTTCACCTT CACGAACTCG GTGTTAGGGA ACTTCTTAGC TCCCTCGACA
  181 ATAGCGTTGG AAAAGAACCC AGGGTCGGAC TCGATGAACA TCTTAGGCAG ATCGTCGCTG
  241 GCCCGAAGGT AGGCGTTGTA GTTGCGGACA ATCTGGACGA CGTCGGGCTT GCCTCCCTTA
  301 ACGAGAGGGA TCTCGCGAGG CCAGGAGAGG GTAGGCCGTC TAACCTCGCC CTTCTCCTTG
  361 AATGGCTCCA GGTAGGCAGC GAACTCCTCA GGCTCCAGTT TCCGCATGAT CTTGCTTGGG
  421 AGCATGGTCT CGACGAAGAA GTTATTCTCA AGCACCATTT TCTCGCCCTC TTCGCTCTTG
  481 ATCAGGGCGA TATCCTCCTC GATGTCAGGC CACTCGTCCC AGGACTCGAT CACGTCCACG
  541 ACACTCTCAG CATGGACGAT GGCCTTGATC TTGTCTTGGT GCTCGTAGGA GTAGTGAAAG
  601 GCCAGACAAG CCCCCCAGTC GTGGCCCACA AAGATGATTT TCTTTGGAAG GTTCAGCAGC
  661 TCGAACCAAG CGGTGAGGTA CTTGTAGTGA TCCAGGAGGC GATATGAGCC ATTCCCGCTC
  721 TTGCCGGACT TACCCATTCC GATCAGATCA GGGATGATGC ATCTAGCCAC GGGCTCGATG
  781 TGAGGCACGA CGTGCCTCCA CAGGTAGCTG GAGGCAGCGT TACCATGCAG AAAAATCACG
  841 GCGTTCTCGG CGTGCTTCTC GGAATCATAG TAGTTGATGA AGGAGTCCAG CACGTTCATT
  901 TGCTTGCAGC GAGCCCACCA CTGAGGCCCA GTGATCATGC GTTTGCGTTG CTCGGGGTCG
  961 TACACCTTGG AAAGCCATGG GTGGGCTAGC CCTATAGTGA GTCGGTATTT AAGTACTCTA
 1021 GCCCTTAAGA GCCTGGTAGT GGAACTGGGG AGTTGACACT TGTTGAGAAG AAAGGCAAGC
1081 TTGGTAGTTC AGTAAGACCA ATTACG
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

reference Wu, B.J., Human HSP70 promoter contains at least two distinct regulatory domains. Proc. Natl. Acad. Sci. USA. 83 (3): 629-633 (1986). PMID 3456160.