RDB05523 - pKM2L-phHIF1A

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pKM2L-phHIF1A (#RDB05523)

Promoter Bank clone, Human hypoxia-inducible factor 1 alpha subunit (HIF1A) promoter

Alternative name	p1938-16F
Clone info.	PCR amplified human hypoxia-inducible factor 1 alpha subunit (HIF1A) promoter sequence was inserted into a Renilla luciferase reporter vector pKM2L (RDB04026). Cloned fragment: 61692498 to 61695674(NC_000014.9); relatively -2903 to +274, where +1 corresponds to 1 nt of NM_001530.3.
Comment	PCR cloning, forward primer, 1938 F2: 5' tgcatttcaccttcaaaaggctca 3'; reverse primer, 1938 R2: 5' acccctcgtgagactagagagaag 3' Entire sequence of promoter region has not been confirmed.
Vector backbone	pKM2L (RDB04026) (Plasmid)
Size of vector backbone	4.2 kb
Selectable markers	Kan^r
Gene/insert name	Human hypoxia-inducible factor 1, alpha subunit genomic DNA
Depositor\|Developer	DNA Bank, \|

Sequence	RDB05523z.seq

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The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.

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Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	1. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. 2. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. Additional terms and conditions: The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.

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Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB05523	pKM2L-phHIF1A	DNA solution

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pKM2L-phHIF1A was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB05523).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB13521_A5Ipp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: M13_-40 Sequence file: RDB13521_A5Ipa.seq
>05523_13521_A5Ip_M13(-40)_A05_02.ab1 1 AGGGCAAAGG CAAGGGTTGC ATGCCTGCAG GTCATTTAAA TAGGGATAAA CAGGGTAATG 61 ATTAAGATCT CGAGCTCTAA GGGGCACGTA GGGCATGCAC TAGTGGCTGC ATTTCACCTT 121 CAAAAGGCTC AATGCTCTGT ATTATTTAGT AGTAATCAAA ATTTCAAGTT TTACTTAACC 181 TCCTGATTCA CTGCCCAATT TCCTAATAAA TACGGGCTAA GGGTCAATGG GGTCATTTGC 241 AAGTAATCTT GTAGTCTACT CAGAAAGTTC TGCAAAGTTA GAAAGTGATT AAATGACTGT 301 TTGTTAAGAT ATACTTACAT AGTAATAACC TAAATGCATT TGTTAAGTGG TTGTAGAGAG 361 AGGGATTTAA AATTTTATCC TATATGAAAT TTTCCTTTTT GGTGTCTGTT ATTTAATAGG 421 ATTGTTTGAA TTAGGGGATA CTATTTGGTG CCTTTGTAAC TATACGAAAA TTAGTTGGTT 481 GAATATTACT GCTTTCCATG TTCATATTTA TATTTGTATA GACATATATA TATATACACA 541 TATACTACTT TCCTTTCCAT TTTCATATTT ATATTTGTGT ATACACATAT ACATAAACAT 601 ATATTTTATA CATTTTTGAA AAGGAAAATT AACTTAAGGG CATATTTAAT GAATATTCAA 661 AAATTTTTTT GCTGATCAAA TTATCATTCT GCTTTAAACT TTTGAAATGA TCCAAAAAAA 721 TTTTAAATGA CTTAGATTTA CTGTTACAAA ATGCTTGTCT TTTGATGTCA CAAACATTAT 781 ATACTATAAT CACTGGCCAG AGAGAATTGC TATAAGTATA ATGAAAAGGG AAATGATGGA 841 AGATCTCTGC AGCTATCCTC ATAAATGAGG GTGGGGAACA CGATGGGCAG TTCCAAAGTT 901 GAAAATAGAG AATATATGTG GGATTTATAT TAACATAATT GGTATTCTTG GATAGTAAAA 961 ATGGCTAAAA CTGTAGGAGA AAGCCCGAAG TAATTACTGG TTAACAGAGG GAATTAAATT 1021 TTGAGGGGCA AATAATAATG ATGATAGGCC AGGGCACTGT GGGCTCATTG CCCTGAAAAT 1081 CCCAGCACCT TTGGGGAACC GCGAAGGCGA GACCGGAACC ACCTTGAGGG TCAGGGAGGT 1141 TCGAGGAGCA ACCCTGGGCA AACATTGGGT GAATAAACCT TCGCGTCTTC C //
Primer: phRLR2 Sequence file: RDB13521_A5Ipb.seq
>05523_13521_A5Ip_phRLR2_A11_02.ab1 1 ACTTCCTGTC TCGACAGCCC AGTTTCTATT GGTCTCCTTA AACCTGTCTT GTAACCTTGA 61 TACTTACCTG CCCAGTGCCT CACGACCAAC TTCTGCAGTA CCCGGGTGAA TTCTACGCGT 121 CGACAGGCAC CCCTCGTGAG ACTAGAGAGA AGCGGGCGGC AATCGCCGGG GTCCGGGCCC 181 GGCTCCGAGC CTCTCCTCAG GTGGCTTGTC AGGGCCCAGC CCGACACACT GGCCGAAGCG 241 ACGAAGAGGG TGATCCTCCT GTCCCCTCAG ACGAGGCAGC ACTGTGCACT GAGGAGCTGA 301 GGCAGCGTCA GGGGGCGGGC AAGGGCGGAG GCGCGCTCGG GCGCGCGGGG AGGGGAGAGG 361 GGGC //
Primer: pAxCALNL_F1 Sequence file: RDB13521_A5Ipc.seq
>05523_13521_A5Ip_pAxCALNL_F1_B11_05.ab1 1 ACCAAGGAAA TCTTATCATG TCTGCTCGAA GCGGCCGCTC TAGAATTACT GCTCGTTCTT 61 CAGCACGCGC TCCACGAAGC TCTTGATGTA CTTACCCATT TCATCTGGAG CGTCCTCCTG 121 GCTGAAGTGG AGGCCCTTCA CCTTCACGAA CTCGGTGTTA GGGAACTTCT TAGCTCCCTC 181 GACAATAGCG TTGGAAAAGA ACCCAGGGTC GGACTCGATG AACATCTTAG GCAGATCGTC 241 GCTGGCCCGA AGGTAGGCGT TGTAGTTGCG GACAATCTGG ACGACGTCGG GCTTGCCTCC 301 CTTAACGAGA GGGATCTCGC GAGGCCAGGA GAGGGTAGGC CGTCTAACCT CGCCCTTCTC 361 CTTGAATGGC TCCAGGTAGG CAGCGAACTC CTCAGGCTCC AGTTTCCGCA TGATCTTGCT 421 TGGGAGCATG GTCTCGACGA AGAAGTTATT CTCAAGCACC ATTTTCTCGC CCTCTTCGCT 481 CTTGATCAGG GCGATATCCT CCTCGATGTC AGGCCACTCG TCCCAGGACT CGATCACGTC 541 CACGACACTC TCAGCATGGA CGATGGCCTT GATCTTGTCT TGGTGCTCGT AGGAGTAGTG 601 AAAGGCCAGA CAAGCCCCCC AGTCGTGGCC CACAAAGATG ATTTTCTTTG GAAGGTTCAG 661 CAGCTCGAAC CAAGCGGTGA GGTACTTGTA GTGATCCAGG AGGCGATATG AGCCATTCCC 721 GCTCTTGCCG GACTTACCCA TTCCGATCAG ATCAGGGATG ATGCATCTAG CCACGGGCTC 781 GATGTGAGGC ACGACGTGCC TCCACAGGTA GCTGGAGGCA GCGTTACCAT GCAGAAAAAT 841 CACGGCGTTC TCGGCGTGCT TCTCGGAATC ATAGTAGTTG ATGAAAGGAG TCCAGCACGT 901 TCATTTGCTT GCAGCGAGCC CACCACTGAG CCCAGTGATC ATGCGTTTGC GTTGCTCGGG 961 GTCGTACACC TTGGAGCCAT GGTGGCTAGC CTATAGTGAG TCGTATTAAG TACTCTAGCC 1021 TTTAGAGCTG TAATTGAACT GGGGAGTGGA CACCTGTGAG AGAAGGCAAG TGATGTCAGT 1081 AGACATAGGT GCCTATCAGA AACCGCAGAG TCCTTCTCTG TCTCCGACAA GCAGTTCTAA 1141 TTGCTTCTCT AACCTGTTC //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

reference	Minet, E., HIF1A gene transcription is dependent on a core promoter sequence encompassing activating and inhibiting sequences located upstream from the transcription initiation site and cis elements located within the 5'UTR. Biochem. Biophys. Res. Commun. 261 (2): 534-540 (1999). PMID 10425220.

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Other resources in our bank

human hypoxia-inducible factor 1, alpha subunit

External Database
Human hypoxia-inducible factor 1, alpha subunit

Reference sequence