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pKM2L-phOAT (#RDB05513)

Promoter Bank clone, Human ornithine aminotransferase (OAT) promoter

Alternative name p1927-13F
Clone info. PCR amplified human ornithine aminotransferase (OAT) promoter sequence was inserted into a Renilla luciferase reporter vector pKM2L (RDB04026). Genomic sequence from 91451 to 90009 of AL445237.16 (reverse direction) corresponding -1407 to +36 (the transcription start site is designated +1. Fig.1 of Engelhardt, J.F. J. Biol. Chem. 266 (2), 752-758, 1991) is cloned. Alternatively, 124420331 to 124418889(NC_000010.11); relatively -1355 to +88, where +1 corresponds to 1 nt of NM_000274.3 was cloned.
Comment PCR cloning, forward primer, 1927 F2: 5' tccagctccttcccatgcagttgct 3'; reverse primer, 1927 R2: 5' acaaccgggtacacgcggcgtctat 3' Entire sequence of promoter region has not been confirmed.
Vector backbone pKM2L (RDB04026) (Plasmid)
Size of vector backbone 4.2 kb
Selectable markers Kan^r
Gene/insert name Human ornithine aminotransferase (gyrate atrophy) genomic DNA
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RDB05513 pKM2L-phOAT DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pKM2L-phOAT was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB05513).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB05513_A6Iup1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: M13_-40
Sequence file: RDB05513_A6Iua.seq check
>05513_05513_A6Iu_M13(-40)_D04_10_ABI24.ab1
    1 AGCAGTCAGC TTGCATGCCT GCAGGTCATT TAAATAGGGA TAACAGGGTA ATGATTAAGA
   61 TCTCGAGCTC TAAGCTTCAC ATATGCATGC ACTAGTGGCT CCAGCTCCTT CCCATGCAGT
  121 TGCTGATGAC TTTGGTGACC AGATAGCCTT TCCCACACTC CTCTCTTTGG GTTCTGGAAT
  181 TCTGGAAACC CCACTTGGAG GCCGTTGGGC CTAGGATGGA TAATGGCCCT CGCTGGTGCA
  241 TGAGACCCAG GTTATTTCTC CATCCCTTGT GGTTTACTTG CCTACATTGA TTGATTGATT
  301 GGTTGATTAA GATGGTCTTG CTTTGTTGCC CAGGCTGGAG TGCAGTGGTG CAGTCATAGG
  361 TCACTGCAGC CTCAACCTCC TGGGCTCAAG TGATCCTCCC ACCTCAGCCT TTGGAGTAGC
  421 TGGGAACATA GGTGTGCCAC CACACCAGAT TAATTTTTAT TTTTTTTTGT GGAGACGGGG
  481 TCTCACTGTG TCGCCCAGGC TGGTCTGGAA CTCCTGGGAT CAAGCAATCC TCCTGCCTCG
  541 GTCTGCCAAA GTGCTGGGAT TACAGGCATG AACCACGGGC CCCCACCACC ACGTTTTAGG
  601 AAATGTTCAC ATTGGAGTGT GCCCTCTGTT TCCAGCTGGG CCCCCGACTG GTCATTGTGG
  661 TCTAATGGAG TGTGGTGCCG GCAGCTGCCA GCGCCTGCCC TCTGAGAGGG GTGGCGAGGG
  721 GCACACAGGA CCAGACTGCC CCTACTGTTG GATGACTGGG TAAGTCGCTT TCCTTCTCTG
  781 GGTCTCTGTT TTCCAATATT TAACATCTTG TTGTGGGCTA AGTGAAGTCC AAGTCCCTTC
  841 CGATTCTAAC ATGAGGTTGT CCCGGAATAT CTATTTTGTG TTCTGAATTT AATAACCGGC
  901 TGTGGAGGGC ACCACAGGCA AATCTGTACT GTGAGTGGGT TGCTGCCACT CAATTATCTG
  961 ACAGCAGCTG CGAACAGGTG GGCAGTGTGT TTTCCATAGG CAGCGACGAC TTTTGGGAGG
 1021 TGGATTCAGA GGCACAACTA AGCCGCCGCG GCGCATCAAG ATCAAGGGCA TGAGCTCCCG
1081 CAGCTGTGCT GGAGCCAGAA GCTGGGGGCC TGATCCCTCC CCTTCCAGGG AGCAC
//
Primer: phRLR2
Sequence file: RDB05513_A6Iub.seq check
>05513_05513_A6Iu_phRLR2_E04_13_ABI24.ab1
    1 AACTCCTGTC TCGACAGCCC AGTTTCTATT GGTCTCCTTA AACCTGTCTT GTAACCTTGA
   61 TACTTACCTG CCCAGTGCCT CACGACCAAC TTCTGCAGTA CCCGGGTGAA TTCTACGCGT
  121 CGACAGGCAC AACCGGGTAC ACGCGACGTC TATGAAGCGC AACAGTGACG CCGGAGCGTC
  181 ACTGCAACTT AAAGGCTGAT TCTGCCCCGC CCCCAAGACA CCCTCGCCGC TCATTGGCTG
  241 GGGCGGAGGG CAGGATACCA ATCGCGTGGG TCCCGTCCTC CGCCCCGCCC TCCGGCCGCG
  301 CATCAAGGAT CTTTGCTCCT CCCGCGGATT CGATCCGCGC TCGCGCCTCC TGAGCCCGCC
  361 GGCCTGTACC TGAGCTCAGG CCCCTCGGTC GCCTCCTCTG GCGGGACGAG AAGAGTCCTT
  421 GTCCGCCCTT GCTGACGAGA AGACTGAGGC CCGAAAAGGA AAAGTCTGTT GGGCCCCCCA
  481 CTGCGCGGGC CGGGTGTGGC TGGAGGCCGA GAGACGTGAG GCTGGACCGC CCGCCCTGTG
  541 CTCCTGGAGG GAGGGACCAG CCCCAGCTCT GCCCCAGCCA CAGCTGCGGG AGCCCCATGC
  601 CCTTGAACCC TGATGCGCCG CGCGGCTTAG TTGTGCCTCT GAATCCACCT CCAAAAGTCG
  661 TCGCTGCCTA TGGAAAACAC ACTGCCCACC TGTTCGCAGC TGCTGTCAGA TAATTGAGTG
  721 GCAGCAACCC ACTCACAGTA CAGATTTGCC TGTGGTGCCC TCCACAGCCG GTTATTAAAT
  781 TCAGAACACA AAATAGATAT TCCGGGACAA CCTCATGTTA GAATCGGAAG GGACTTGGAC
  841 TTCACTTAGC CCACAACAAG ATGTTAAATA TTGGAAAACA GAGACCCAGA GAAGGAAAGC
  901 GACTTACCCA GTCATCCAAC AGTAGGGGCA GTCTGGTCCT GTGTGCCCCT CGCCACCCCT
  961 CTCAGAGGGC AGGCGCTGGC AGCTGCCGGC ACCACACTCC ATTAGACCAC AATGACCAGT
 1021 CGGGGGCCCA GCTGGGAAAC AGAGGCACAC TCCAATGTGA ACATTTCCTA AAACGTTGAT
 1081 GTTGGGGGGC CCGTGGATCA ATGCGTGTAT CCCAAGCACT TTGGCAGACG AAGTAGGAGG
1141 AATTGGCCTT GGAATT
//
Primer: pAxCALNL_F1
Sequence file: RDB05513_A6Iuc.seq check
>05513_05513_A6Iu_pAxCALNL_F1_F04_16_ABI24.ab1
    1 ACAATCTATC TTATCATGTC TGCTCGAAGC GGCCGCTCTA GAATTACTGC TCGTTCTTCA
   61 GCACGCGCTC CACGAAGCTC TTGATGTACT TACCCATTTC ATCTGGAGCG TCCTCCTGGC
  121 TGAAGTGGAG GCCCTTCACC TTCACGAACT CGGTGTTAGG GAACTTCTTA GCTCCCTCGA
  181 CAATAGCGTT GGAAAAGAAC CCAGGGTCGG ACTCGATGAA CATCTTAGGC AGATCGTCGC
  241 TGGCCCGAAG GTAGGCGTTG TAGTTGCGGA CAATCTGGAC GACGTCGGGC TTGCCTCCCT
  301 TAACGAGAGG GATCTCGCGA GGCCAGGAGA GGGTAGGCCG TCTAACCTCG CCCTTCTCCT
  361 TGAATGGCTC CAGGTAGGCA GCGAACTCCT CAGGCTCCAG TTTCCGCATG ATCTTGCTTG
  421 GGAGCATGGT CTCGACGAAG AAGTTATTCT CAAGCACCAT TTTCTCGCCC TCTTCGCTCT
  481 TGATCAGGGC GATATCCTCC TCGATGTCAG GCCACTCGTC CCAGGACTCG ATCACGTCCA
  541 CGACACTCTC AGCATGGACG ATGGCCTTGA TCTTGTCTTG GTGCTCGTAG GAGTAGTGAA
  601 AGGCCAGACA AGCCCCCCAG TCGTGGCCCA CAAAGATGAT TTTCTTTGGA AGGTTCAGCA
  661 GCTCGAACCA AGCGGTGAGG TACTTGTAGT GATCCAGGAG GCGATATGAG CCATTCCCGC
  721 TCTTGCCGGA CTTACCCATT CCGATCAGAT CAGGGATGAT GCATCTAGCC ACGGGCTCGA
  781 TGTGAGGCAC GACGTGCCTC CACAGGTAGC TGGAGGCAGC GTTACCATGC AGAAAAATCA
  841 CGGCGTTCTC GGCGTGCTTC TCGGAATCAT AGTAGTTGAT GAAGGAGTCC AGCACGTTCA
  901 TTTGCTTGCA GCGAGCCCAC CACTGAGGCC CAGTGATCAT GCGTTTGCGT TGCTCGGGGT
  961 CGTACACCTT GGAAAGCCAT GGGTGGCTAG CCCTATAGTG AGTCGTATTA AGTACTCTAG
 1021 CCCTTAAGAG CTGTAATTTG AACTGGGGAG TGACACCTGT GGTAGAAGAA AGCAAGTCAT
 1081 GTCAGTAGAC CATAGTGCTA TCAGAACGCA GAGTCATCTC TGTCCTCCGA ACAAAGCCCA
1141 AGT
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

reference Engelhardt, J.F., Transcriptional analysis of the human ornithine aminotransferase promoter. J. Biol. Chem. 266 (2): 752-758 (1991). PMID 1845993.