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pKM2L-phPAI1

Promoter Bank clone, Human plasminogen activator inhibitor 1 (PAI-1) promoter

Catalog number RDB05506
Resource name pKM2L-phPAI1
Alternative name p1918-8F
Clone info. PCR amplified human plasminogen activator inhibitor 1 (PAI-1) promoter sequence was inserted into a Renilla luciferase reporter vector pKM2L (RDB04026). Cloned fragment: 101126296 to 101127123 (NC_000007.14); relatively -793 to +35, where +1 corresponds to 1 nt of NM_000602.4.
Comment PCR cloning, forward primer, F6PB0046F: 5' aagcttttaccatggtaacccctgg 3'; reverse primer, F6PB0046R: 5' ctgcagccaaacacagctgtgctc 3' Entire sequence of promoter region has not been confirmed. Known difference (at least) : 1nt (G) insertion between 101126425 to 101126426nt.
Vector backbone pKM2L (RDB04026) (Plasmid)
Size of vector backbone 4.2 kb
Selectable markers Kan^r
Gene/insert name Human plasminogen activator inhibitor 1 (PAI-1) genomic DNA
Depositor|Developer DNA Bank, |
Other clones in our bank

External Database
Human plasminogen activator inhibitor 1 (PAI-1)

          Reference sequence
            X13323, AF386492, AC004876

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR 1. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. 2. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
          Additional terms and conditions for distribution The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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          RDB05506 pKM2L-phPAI1 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Sequence (full) RDB05506zzk02.seq provided by the depositor

          Original reference

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Promoter Collection (Renilla Luciferase) (English text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB19567_B2Cbp1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pBAC_lac_F
          Region: insert 5'
          Sequence file: RDB19567_B2Cba.seq check
          >D05506_19567_B2Cb_3_pBAC_lac_F_F02_17_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN GCTTGCNTGC CTGCAGGTCA TTTAAATAGG GNNAACAGGG
             61 TAATGATTAA GATCTCGAGC TCTAAGCTTC ACATATGCAT GCACTAGTGG CAAGCTTTTA
            121 CCATGGTAAC CCCTGGTCCC GTTCAGCCAC CACCACCCCA CCCAGCACAC CTCCAACCTC
            181 AGCCAGACAA GGTTGTTGAC ACAAGAGAGC CCTCAGGGGC ACAGAGAGAG TCTGGACACG
            241 TGGGGGAGTC AGCCGTGTAT CATCGGAGGC GGCCGGGCAC ATGGCAGGGA TGAGGGAAAG
            301 ACCAAGAGTC CTCTGTTGGG CCCAAGTCCT AGACAGACAA AACCTAGACA ATCACGTGGC
            361 TGGCTGCATG CCCTGTGGCT GTTGGGCTGG GCCCAGGAGG AGGGAGGGGC GCTCTTTCCT
            421 GGAGGTGGTC CAGAGCACCG GGTGGACAGC CCTGGGGGAA AACTTCCACG TTTTGATGGA
            481 GGTTATCTTT GATAACTCCA CAGTGACCTG GTTCGCCAAA GGAAAAGCAG GCAACGTGAG
            541 CTGTTTTTTT TTTCTCCAAG CTGAACACTA GGGGTCCTAG GCTTTTTGGG TCACCCGGCA
            601 TGGCAGACAG TCAACCTGGC AGGACATCCG GGAGAGACAG ACACAGGCAG AGGGCAGAAA
            661 GGTCAAGGGA GGTTCTCAGG CCAAGGCTAT TGGGGTTTGC TCAATTGTTC CTGAATGCTC
            721 TTACACACGT ACACACACAG AGCAGCACAC ACACACACAC ACACATGCCT CAGCAAGTCC
            781 CAGANAGGGA NGTGTCGAGG GGGANCNGCT GGCTGTTCAN ANGGANTCCC AGANCCNGTG
            841 AGTGGGTGGG GCTGGAACAT GAGTNCATCT ATTTCCTGNC CNCNTCTGGT ATAAAANGAN
            901 GNAGTGGNCC NCAGANGANC ACAGCTGTGT TTGNNTGCNN NNCTGNCNAC NCNNANAATT
            961 CNCCCNGGNN NNGNANAANN NGGNCGNNAN GNNNTGGNNN NNNNNNTNTN NNNNNNANNN
          1021 NNNNNNTNNN NNNNNNN
          //
          Primer: phRLR2primer
          Region: chimeric intron,insert 3'
          Sequence file: RDB19567_B2Cbb.seq check
          >D05506_19567_B2Cb_3_phRLR2primer_G02_20_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNGTTTCTAT TGGTCTCCTT AAACCTGTCT TGTAACCTTG
             61 ATACTTACCT GCCCAGTGCC TCACGACCAA CTTCTGCAGT ACCCGGGTGA ATTCTACGCG
            121 TCGACAGGCC TGCAGCCAAA CACAGCTGTG CTCCTCTGTG GGCCACTGCC TCCTTTTATA
            181 CCAGATGTGG GCAGGAAATA GATGAACTCA TGTTCCAGCC CCACCCACTC ACTGGCTCTG
            241 GGAGTCCGTC TGAACAGCCA GCGGGTCCCC CTCGACACCT CCCTCTCTGG GACTTGCTGA
            301 GGCATGTGTG TGTGTGTGTG TGTGCTGCTC TGTGTGTGTA CGTGTGTAAG AGCATTCAGG
            361 AACAATTGAG CAAACCCCAA TAGCCTTGGC CTGAGAACCT CCCTTGACCT TTCTGCCCTC
            421 TGCCTGTGTC TGTCTCTCCC GGATGTCCTG CCAGGTTGAC TGTCTGCCAT GCCGGGTGAC
            481 CCAAAAAGCC TAGGACCCCT AGTGTTCAGC TTGGAGAAAA AAAAAACAGC TCACGTTGCC
            541 TGCTTTTCCT TTGGCGAACC AGGTCACTGT GGAGTTATCA AAGATAACCT CCATCAAAAC
            601 GTGGAAGTTT TCCCCCAGGG CTGTCCACCC GGTGCTCTGG ACCACCTCCA GGAAAGAGCG
            661 CCCCTCCCTC CTCCTGGGCC CAGCCCAACA GCCACAGGGC ATGCAGCCAG CCACGTGATT
            721 GTCTAGGTTT TGTCTGTCTA GGANTTGGGC CCAACAGANG ANTCTTGGTC TTTCCCTCNT
            781 CCCTGCCATG TGCCCGGNCG CCTNCGATGA TACACGGCTG ANTCCCCCAC GTGNNCAGAN
            841 TCTCTCTGTG CCCCNGANGG CTCTCTNGNG TCNACNACCN TGTCTGGNTG NNNTGNANNN
            901 GNGCTGGGNG GGGNGGNGGN GGNTGAACGG GNNCAGGGGT TNNNNNNNNA AAANCTTGNN
          961 NCTANNNNNT GNNTNTGTNN NNNTNANNNN TCGANNNNNT NNNCNNTANN NNNNNNNN
          //
          Primer: SV40pA_R
          Region: SV40 polyA,hRluc 3'
          Sequence file: RDB19567_B2Cbc.seq check
          >D05506_19567_B2Cb_3_SV40pA_R_H02_23_ABI24.ab1
              1 NNNNNNNNNN CATCNNNNNN TTNNNNAATA AAGCATTTTT TTNNCTGCAT TCTAGTTGTG
             61 GTTTGTCCAA ACTCATCAAT GTATCTTATC ATGTCTGCTC GAAGCGGCCG CTCTAGAATT
            121 ACTGCTCGTT CTTCAGCACG CGCTCCACGA AGCTCTTGAT GTACTTACCC ATTTCATCTG
            181 GAGCGTCCTC CTGGCTGAAG TGGAGGCCCT TCACCTTCAC GAACTCGGTG TTAGGGAACT
            241 TCTTAGCTCC CTCGACAATA GCGTTGGAAA AGAACCCAGG GTCGGACTCG ATGAACATCT
            301 TAGGCAGATC GTCGCTGGCC CGAAGGTAGG CGTTGTAGTT GCGGACAATC TGGACGACGT
            361 CGGGCTTGCC TCCCTTAACG AGAGGGATCT CGCGAGGCCA GGAGAGGGTA GGCCGTCTAA
            421 CCTCGCCCTT CTCCTTGAAT GGCTCCAGGT AGGCAGCGAA CTCCTCAGGC TCCAGTTTCC
            481 GCATGATCTT GCTTGGGAGC ATGGTCTCGA CGAAGAAGTT ATTCTCAAGC ACCATTTTCT
            541 CGCCCTCTTC GCTCTTGATC AGGGCGATAT CCTCCTCGAT GTCAGGCCAC TCGTCCCAGG
            601 ACTCGATCAC GTCCACGACA CTCTCAGCAT GGACGATGGC CTTGATCTTG TCTTGGTGCT
            661 CGTAGGANTA GTGAAAGGNC AGACAAGCCC NNCAGTCGTG GCCCACAAAG ANGATTTTCT
            721 TTGNAANGNT CANCAGCTCG AACCAAGCGG TGAGGNACTT GTAGTGNTNC NGGANGNGAT
            781 ATGAGCCNTT CCCGCTCTTG NCGGANTTAC CCNTTNCNAT CNNATCNNNN NGANGCATCT
            841 AGCCNCGGGC TCNNNGNGAN GNACGANNNG NCTNCNCNNN TANCTNNAGN NAGCGNNNCN
            901 NTGNNNAAAA ATCNCNGNNN NNNCNGNNNG NTNNNNNNNN NCNTNNNANN NGANGNNNNN
          961 TNCAGCNNNN NNNNTNNNNN NNNNN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles


          2022.05.18

          GNP_filter3_RDBDEP_html_220516.pl