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pKM2L-phPAI1 (#RDB05506)

Promoter Bank clone, Human plasminogen activator inhibitor 1 (PAI-1) promoter

Alternative name p1918-8F
Clone info. PCR amplified human plasminogen activator inhibitor 1 (PAI-1) promoter sequence was inserted into a Renilla luciferase reporter vector pKM2L (RDB04026). Cloned fragment: 101126296 to 101127123 (NC_000007.14); relatively -793 to +35, where +1 corresponds to 1 nt of NM_000602.4.
Comment PCR cloning, forward primer, F6PB0046F: 5' aagcttttaccatggtaacccctgg 3'; reverse primer, F6PB0046R: 5' ctgcagccaaacacagctgtgctc 3' Entire sequence of promoter region has not been confirmed. Known difference (at least) : 1nt (G) insertion between 101126425 to 101126426nt.
Vector backbone pKM2L (RDB04026) (Plasmid)
Size of vector backbone 4.2 kb
Selectable markers Kan^r
Gene/insert name Human plasminogen activator inhibitor 1 (PAI-1) genomic DNA
Depositor|Developer DNA Bank, |
 
Sequence (full) RDB05506zzk02.seq provided by the depositor

Distribution information

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The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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RDB05506 pKM2L-phPAI1 DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pKM2L-phPAI1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB05506).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB19567_B2Cbp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pBAC_lac_F (Pr0544)
Region: insert 5'
Sequence file: RDB19567_B2Cba.seq check
>D05506_19567_B2Cb_3_pBAC_lac_F_F02_17_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN GCTTGCNTGC CTGCAGGTCA TTTAAATAGG GNNAACAGGG
   61 TAATGATTAA GATCTCGAGC TCTAAGCTTC ACATATGCAT GCACTAGTGG CAAGCTTTTA
  121 CCATGGTAAC CCCTGGTCCC GTTCAGCCAC CACCACCCCA CCCAGCACAC CTCCAACCTC
  181 AGCCAGACAA GGTTGTTGAC ACAAGAGAGC CCTCAGGGGC ACAGAGAGAG TCTGGACACG
  241 TGGGGGAGTC AGCCGTGTAT CATCGGAGGC GGCCGGGCAC ATGGCAGGGA TGAGGGAAAG
  301 ACCAAGAGTC CTCTGTTGGG CCCAAGTCCT AGACAGACAA AACCTAGACA ATCACGTGGC
  361 TGGCTGCATG CCCTGTGGCT GTTGGGCTGG GCCCAGGAGG AGGGAGGGGC GCTCTTTCCT
  421 GGAGGTGGTC CAGAGCACCG GGTGGACAGC CCTGGGGGAA AACTTCCACG TTTTGATGGA
  481 GGTTATCTTT GATAACTCCA CAGTGACCTG GTTCGCCAAA GGAAAAGCAG GCAACGTGAG
  541 CTGTTTTTTT TTTCTCCAAG CTGAACACTA GGGGTCCTAG GCTTTTTGGG TCACCCGGCA
  601 TGGCAGACAG TCAACCTGGC AGGACATCCG GGAGAGACAG ACACAGGCAG AGGGCAGAAA
  661 GGTCAAGGGA GGTTCTCAGG CCAAGGCTAT TGGGGTTTGC TCAATTGTTC CTGAATGCTC
  721 TTACACACGT ACACACACAG AGCAGCACAC ACACACACAC ACACATGCCT CAGCAAGTCC
  781 CAGANAGGGA NGTGTCGAGG GGGANCNGCT GGCTGTTCAN ANGGANTCCC AGANCCNGTG
  841 AGTGGGTGGG GCTGGAACAT GAGTNCATCT ATTTCCTGNC CNCNTCTGGT ATAAAANGAN
  901 GNAGTGGNCC NCAGANGANC ACAGCTGTGT TTGNNTGCNN NNCTGNCNAC NCNNANAATT
  961 CNCCCNGGNN NNGNANAANN NGGNCGNNAN GNNNTGGNNN NNNNNNTNTN NNNNNNANNN
1021 NNNNNNTNNN NNNNNNN
//
Primer: phRLR2primer (Pr0083)
Region: chimeric intron,insert 3'
Sequence file: RDB19567_B2Cbb.seq check
>D05506_19567_B2Cb_3_phRLR2primer_G02_20_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNGTTTCTAT TGGTCTCCTT AAACCTGTCT TGTAACCTTG
   61 ATACTTACCT GCCCAGTGCC TCACGACCAA CTTCTGCAGT ACCCGGGTGA ATTCTACGCG
  121 TCGACAGGCC TGCAGCCAAA CACAGCTGTG CTCCTCTGTG GGCCACTGCC TCCTTTTATA
  181 CCAGATGTGG GCAGGAAATA GATGAACTCA TGTTCCAGCC CCACCCACTC ACTGGCTCTG
  241 GGAGTCCGTC TGAACAGCCA GCGGGTCCCC CTCGACACCT CCCTCTCTGG GACTTGCTGA
  301 GGCATGTGTG TGTGTGTGTG TGTGCTGCTC TGTGTGTGTA CGTGTGTAAG AGCATTCAGG
  361 AACAATTGAG CAAACCCCAA TAGCCTTGGC CTGAGAACCT CCCTTGACCT TTCTGCCCTC
  421 TGCCTGTGTC TGTCTCTCCC GGATGTCCTG CCAGGTTGAC TGTCTGCCAT GCCGGGTGAC
  481 CCAAAAAGCC TAGGACCCCT AGTGTTCAGC TTGGAGAAAA AAAAAACAGC TCACGTTGCC
  541 TGCTTTTCCT TTGGCGAACC AGGTCACTGT GGAGTTATCA AAGATAACCT CCATCAAAAC
  601 GTGGAAGTTT TCCCCCAGGG CTGTCCACCC GGTGCTCTGG ACCACCTCCA GGAAAGAGCG
  661 CCCCTCCCTC CTCCTGGGCC CAGCCCAACA GCCACAGGGC ATGCAGCCAG CCACGTGATT
  721 GTCTAGGTTT TGTCTGTCTA GGANTTGGGC CCAACAGANG ANTCTTGGTC TTTCCCTCNT
  781 CCCTGCCATG TGCCCGGNCG CCTNCGATGA TACACGGCTG ANTCCCCCAC GTGNNCAGAN
  841 TCTCTCTGTG CCCCNGANGG CTCTCTNGNG TCNACNACCN TGTCTGGNTG NNNTGNANNN
  901 GNGCTGGGNG GGGNGGNGGN GGNTGAACGG GNNCAGGGGT TNNNNNNNNA AAANCTTGNN
961 NCTANNNNNT GNNTNTGTNN NNNTNANNNN TCGANNNNNT NNNCNNTANN NNNNNNNN
//
Primer: SV40pA_R (Pr0882)
Region: SV40 polyA,hRluc 3'
Sequence file: RDB19567_B2Cbc.seq check
>D05506_19567_B2Cb_3_SV40pA_R_H02_23_ABI24.ab1
    1 NNNNNNNNNN CATCNNNNNN TTNNNNAATA AAGCATTTTT TTNNCTGCAT TCTAGTTGTG
   61 GTTTGTCCAA ACTCATCAAT GTATCTTATC ATGTCTGCTC GAAGCGGCCG CTCTAGAATT
  121 ACTGCTCGTT CTTCAGCACG CGCTCCACGA AGCTCTTGAT GTACTTACCC ATTTCATCTG
  181 GAGCGTCCTC CTGGCTGAAG TGGAGGCCCT TCACCTTCAC GAACTCGGTG TTAGGGAACT
  241 TCTTAGCTCC CTCGACAATA GCGTTGGAAA AGAACCCAGG GTCGGACTCG ATGAACATCT
  301 TAGGCAGATC GTCGCTGGCC CGAAGGTAGG CGTTGTAGTT GCGGACAATC TGGACGACGT
  361 CGGGCTTGCC TCCCTTAACG AGAGGGATCT CGCGAGGCCA GGAGAGGGTA GGCCGTCTAA
  421 CCTCGCCCTT CTCCTTGAAT GGCTCCAGGT AGGCAGCGAA CTCCTCAGGC TCCAGTTTCC
  481 GCATGATCTT GCTTGGGAGC ATGGTCTCGA CGAAGAAGTT ATTCTCAAGC ACCATTTTCT
  541 CGCCCTCTTC GCTCTTGATC AGGGCGATAT CCTCCTCGAT GTCAGGCCAC TCGTCCCAGG
  601 ACTCGATCAC GTCCACGACA CTCTCAGCAT GGACGATGGC CTTGATCTTG TCTTGGTGCT
  661 CGTAGGANTA GTGAAAGGNC AGACAAGCCC NNCAGTCGTG GCCCACAAAG ANGATTTTCT
  721 TTGNAANGNT CANCAGCTCG AACCAAGCGG TGAGGNACTT GTAGTGNTNC NGGANGNGAT
  781 ATGAGCCNTT CCCGCTCTTG NCGGANTTAC CCNTTNCNAT CNNATCNNNN NGANGCATCT
  841 AGCCNCGGGC TCNNNGNGAN GNACGANNNG NCTNCNCNNN TANCTNNAGN NAGCGNNNCN
  901 NTGNNNAAAA ATCNCNGNNN NNNCNGNNNG NTNNNNNNNN NCNTNNNANN NGANGNNNNN
961 TNCAGCNNNN NNNNTNNNNN NNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles