RDB05503 - pKM2L-phPOMC

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pKM2L-phPOMC (#RDB05503)

Promoter Bank clone, Human pro-opiomelanocortin (POMC) promoter

Alternative name	p1914-3F
Clone info.	PCR amplified human pro-opiomelanocortin (POMC) promoter sequence was inserted into a Renilla luciferase reporter vector pKM2L (RDB04026). Cloned fragment: 25169263 to 25168520(NC_000002.12); relatively -412 to +332, where +1 corresponds to 1 nt of NM_001035256.2.
Comment	PCR cloning, forward primer, B6PB0042F: 5' ctgctcttcacagcatcaccctctc 3'; reverse primer, B6PB0042R: 5' gagggcggggacgctcggcggc 3'; Entire sequence of promoter region has not been confirmed. Promoter activity has not been analyzed.
Vector backbone	pKM2L (RDB04026) (Plasmid)
Size of vector backbone	4.2 kb
Selectable markers	Kan^r
Gene/insert name	Human proopiomelanocortin (adrenocorticotropin/ beta-lipotropin/ alpha-melanocyte stimulating hormone/ beta-melanocyte stimulating hormone/ beta-endorphin) genomic DNA
Depositor\|Developer	DNA Bank, \|

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Terms and conditions for distribution 1. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. 2. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
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The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.

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Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	1. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. 2. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. Additional terms and conditions: The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件	1. 本件リソースは学術研究機関にのみ提供し、学術研究にのみ利用することができる。　2. 謝辞の表明を必要とする。 MTAに書く付加的使用条件：本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB05503	pKM2L-phPOMC	DNA solution	JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pKM2L-phPOMC was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB05503).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB05503_A4Lm.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: M13_-40 Sequence file: RDB05503_A4Lma.seq
>05503_05503_A4Lm_3_M13(-40)_C08_08.ab1 1 CCAGCGGCTC AGCTTGCATG CCTGCAGGTC ATTTAAATAG GGATAACAGC GCGTAATGAT 61 TAAGATCTCG AGCTCTAAGC TTCACATATG CATGCACTAG TGGCCTGCTC TTCACAGCAT 121 CACCCTCTCC CCATTTAATG GTTTAGGTTA ACAGGACTTT TTCCTTGAGG CTTGGGACAC 181 GGAAGGGAGC CTCCCCTAAA CCAGGCCCTT GGAGAGCAGG CCCCAGGGGA GCAGTGCAAC 241 TCACCTTCAC ACCCACAAGA CGGCTCCTGA CTTCTGCTCC CTCCTCCCCT CCCCAAAGTG 301 GAACAGAGAG AATATGATTC CCCACGACTT CCACATCACA GTTTCCAAAC AATGGGGAAA 361 TCGGAGGCCT CCCCGTGTGC AGACGGTGAT ATTTACCGCC AAATGCGAAC CAGGCAGATG 421 CCAGCCCCAG CACGCACGCA GGTAACTTCA CCCTCGCCTC AACGACCTCA GAGGCTGCCC 481 GGCCTGCCCC ACACGGGGGT GCTAAGCCTC CCGCCCGTTC TAAGCGGAGA CCCAACGCCA 541 TCCATAATTA AGTTCTTCCT GAGGGCGAGC GGCCAGGTGC GCCTTCGGCA GGACAGTGCT 601 AATTCCAGCC CCTTTCCAGC GCGTCTCCCC GCGCTCGTCC CCCGTCTGGA AGCCCCCCTC 661 CCACGCCCCG CGGCCCCCCT TCCCCTGGCC CGGGGAGCTG CTCCTTGTGC TGCCGGGAAG 721 GTCAAAGTCC CGCGCCCACC AGGAGAGCTC GGCAAGTATA TAAGGACAGA GGAGCGCGGG 781 ACCAAGCGGC GGCGAAGGAG GGGAAGAAGA GCCGCGACCG AGAGAGGCCG CCGAGCGTCC 841 CCGCCCTCGC CTGTCGACGC GTAGAATTCA CCCGGGTACT GCAGAAGTTG GTCGTGAGGC 901 ACTGGGCAGG TAAGTATCAA GGTTACAAGA CAGGTTTAAG GAGACCAATA GAAACTGGGC 961 TTGTCGAGAC AGAGAAGACT CTTGCGTTTC TGATAGGCAC CTATTGGTCT TACTGACATC 1021 CACTTTGCCT TTCTCTCCAC AGGTGTCCAC TCCCAGTTCA AATTACAGCT CTTTAAAGCT 1081 AGAGTACTTT AATACGACTC ACTATAGGCT AGCCACCAAT GCCTCACGTG TACGACCCCG 1141 AGCCAACGGC CAAAACGCCA ATTGATTCCA CCCTTTG //
Primer: phRLR2 Sequence file: RDB05503_A4Lmb.seq
>05503_05503_A4Lm_3_phRLR2_D08_11.ab1 1 AACTCCTATT GCAGCCCAGT TTCTATTGGT CTCCTTAAAC CTGTCTTGTA ACCTTGATAC 61 TTACCTGCCC AGTGCCTCAC GACCAACTTC TGCAGTACCC GGGTGAATTC TACGCGTCGA 121 CAGGCGAGGG CGGGGACGCT CGGCGGCCTC TCTCGGTCGC GGCTCTTCTT CCCCTCCTTC 181 GCCGCCGCTT GGTCCCGCGC TCCTCTGTCC TTATATACTT GCCGAGCTCT CCTGGTGGGC 241 GCGGGACTTT GACCTTCCCG GCAGCACAAG GAGCAGCTCC CCGGGCCAGG GGAAGGGGGG 301 CCGCGGGGCG TGGGAGGGGG GCTTCCAGAC GGGGGACGAG CGCGGGGAGA CGCGCTGGAA 361 AGGGGCTGGA ATTAGCACTG TCCTGCCGAA GGCGCACCTG GCCGCTCGCC CTCAGGAAGA 421 ACTTAATTAT GGATGGCGTT GGGTCTCCGC TTAGAACGGG CGGGAGGCTT AGCACCCCCG 481 TGTGGGGCAG GCCGGGCAGC CTCTGAGGTC GTTGAGGCGA GGGTGAAGTT ACCTGCGTGC 541 GTGCTGGGGC TGGCATCTGC CTGGTTCGCA TTTGGCGGTA AATATCACCG TCTGCACACG 601 GGGAGGCCTC CGATTTCCCC ATTGTTTGGA AACTGTGATG TGGAAGTCGT GGGGAATCAT 661 ATTCTCTCTG TTCCACTTTG GGGAGGGGAG GAGGGAGCAG AAGTCAGGAG CCGTCTTGTG 721 GGTGTGAAGG TGAGTTGCAC TGCTCCCCTG GGGCCTGCTC TCCAAGGGCC TGGTTTAGGG 781 GAGGCTCCCT TCCGTGTCCC AAGCCTCAAG GAAAAAGTCC TGTTAACCTA AACCATTAAA 841 TGGGGAGAGG GTGATGCTGT GAAGAGCAGG CCACTAGTGC ATGCATATGT GAAGCTTAGA 901 GCTCGAGATC TTAATCATTA CCCTGTTATC CCTATTTAAA TGACCTGCAG GCATGCAAGC 961 TTGGCACTGG GCCGTCGTTT TACAACGTCG TGACTGGGAA AACCCTGGCG TTACCCAACT 1021 TTAATCGCCT TGCAGCACAT CCCCCTTTCG CCAGCTGGCG TATAGCGAGA GGCCCCGCCA 1081 CGATCGCCTT TCCCACAGTT GCGCAGCTGA TGGCCGATGC GATTTTATTC AACAAAGCCG 1141 CCCGATTCCC CGTCCAAAGT CG //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

Resource search

Link

Other resources in our bank

human proopiomelanocortin (adrenocorticotropin/ beta-lipotropin/ alpha-melanocyte stimulating hormone/ beta-melanocyte stimulating hormone/ beta-endorphin)

External Database
Human proopiomelanocortin (adrenocorticotropin/ beta-lipotropin/ alpha-melanocyte stimulating hormone/ beta-melanocyte stimulating hormone/ beta-endorphin)

Reference sequence
J00290