RDB05499 - pKM2L-phSPRR2F

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pKM2L-phSPRR2F (#RDB05499)

Promoter Bank clone, Human small proline-rich protein 2F (SPRR2F) promoter

Alternative name	p1910-1F
Clone info.	PCR amplified human small proline-rich protein 2F (SPRR2F) promoter sequence was inserted into a Renilla luciferase reporter vector pKM2L (RDB04026). Cloned fragment: 153113923 to 153113513(NC_000001.11); relatively -408 to +3, where +1 corresponds to 1 nt of NM_001014450.2.
Comment	PCR cloning, forward primer, F5PB0038F: 5' aaagtagaggtggagatgggttcat 3'; reverse primer, F5PB0038R: 5' tttaggagtcgtgcagcagaaaacc 3'; Entire sequence of promoter region has not been confirmed. Promoter activity has not been analyzed.
Vector backbone	pKM2L (RDB04026) (Plasmid)
Size of vector backbone	4.2 kb
Selectable markers	Kan^r
Gene/insert name	Human small proline-rich protein 2F (SPRR2F) genomic DNA
Depositor\|Developer	DNA Bank, \|

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The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.

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Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	1. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. 2. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. Additional terms and conditions: The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.

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MTAに書く使用条件	1. 本件リソースは学術研究機関にのみ提供し、学術研究にのみ利用することができる。　2. 謝辞の表明を必要とする。 MTAに書く付加的使用条件：本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB05499	pKM2L-phSPRR2F	DNA solution	JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pKM2L-phSPRR2F was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB05499).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB05499_A5Kkp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward Sequence file: RDB05499_A5Kka.seq
>05499_05499_A5Kk_3_M13(-40)_B11_05.ab1 1 CGGCGGCAGC TTGCATGCCT GCAGGTCATT TAAATAGGGA TAACAGGGTA ATGATTAAGA 61 TCTCGAGCTC TAAGCTTCAC ATATGCATGC ACTAGTGGCA AAGTAGAGGT GGAGATGGGT 121 TCATGTACAA CAATATGGCA TTCAGTAAAG TGGATCAGGA CAGGAAGTTT TATGATTTAG 181 GGAAGGTGTA AGACAGGAAA GGATCATTGC CCCCAGTCAA AAAAGAGAGC CCTTGACCAC 241 CAGTTAGAGA ATCCCCCAAG TCCCTCTTTG CCATAGGTCA CTGAAACTGA GATCTAAGGC 301 AGGGCCTCTG TGAGTCAGGA GCACTTAACC CAGTGGGGAG ATCCCAGAAC AGGATATTTC 361 CTAGTTTGAT AATCACTGTC AGGCCAGTTG TGGATAAATT TGCATTTGGC TTAAGAAATT 421 ACTGGATAAG TGTAGCTTTT GGCAGTTACA CTTTCTGCTG GGAGGGGTAG CAGGCCCTAT 481 AAAGAGGTTT TCTGCTGCAC GACTCCTAAA GCCTGTCGAC GCGTAGAATT CACCCGGGTA 541 CTGCAGAAGT TGGTCGTGAG GCACTGGGCA GGTAAGTATC AAGGTTACAA GACAGGTTTA 601 AGGAGACCAA TAGAAACTGG GCTTGTCGAG ACAGAGAAGA CTCTTGCGTT TCTGATAGGC 661 ACCTATTGGT CTTACTGACA TCCACTTTGC CTTTCTCTCC ACAGGTGTCC ACTCCCAGTT 721 CAATTACAGC TCTTAAGGCT AGAGTACTTA ATACGACTCA CTATAGGCTA GCCACCATGG 781 CTTCCAAGGT GTACGACCCC GAGCAACGCA AACGCATGAT CACTGGGCCT CAGTGGTGGG 841 CTCGCTGCAA GCAAATGAAC GTGCTGGACT CCTTCATCAA CTACTATGAT TCCGAGAAGC 901 ACGCCGAGAA CGCCGTGATT TTTCTGCATG GTAACGCTGC CTCCAGCTAC CTGTGGAGGC 961 ACGTCGTGCC TCACATCGAG CCCGTGGCTA GATGCATCAT CCCTGATCTG ATCGGAATGG 1021 GTAAGTCCGG CAAGAGCGGG GAATGGCTCA TATCGCTCTG ATCACTACAA GTACCTTCAC 1081 GCTGATCGAG CTGCTGACCT CGAGAAATCA TCCTTGTGGC CACGAACTGG GGGGCTGGTT 1141 CTTGGCCTTT CGCA //
Primer: CMV pro,5'LTR,HIV-1 psi Sequence file: RDB05499_A5Kkb.seq
>05499_05499_A5Kk_3_pAxCALNL_F1_E11_14.ab1 1 TCAGTATCTT ATCATGTCTG CTCGAAGCGG CCGCTCTAGA ATTACTGCTC GTTCTTCAGC 61 ACGCGCTCCA CGAAGCTCTT GATGTACTTA CCCATTTCAT CTGGAGCGTC CTCCTGGCTG 121 AAGTGGAGGC CCTTCACCTT CACGAACTCG GTGTTAGGGA ACTTCTTAGC TCCCTCGACA 181 ATAGCGTTGG AAAAGAACCC AGGGTCGGAC TCGATGAACA TCTTAGGCAG ATCGTCGCTG 241 GCCCGAAGGT AGGCGTTGTA GTTGCGGACA ATCTGGACGA CGTCGGGCTT GCCTCCCTTA 301 ACGAGAGGGA TCTCGCGAGG CCAGGAGAGG GTAGGCCGTC TAACCTCGCC CTTCTCCTTG 361 AATGGCTCCA GGTAGGCAGC GAACTCCTCA GGCTCCAGTT TCCGCATGAT CTTGCTTGGG 421 AGCATGGTCT CGACGAAGAA GTTATTCTCA AGCACCATTT TCTCGCCCTC TTCGCTCTTG 481 ATCAGGGCGA TATCCTCCTC GATGTCAGGC CACTCGTCCC AGGACTCGAT CACGTCCACG 541 ACACTCTCAG CATGGACGAT GGCCTTGATC TTGTCTTGGT GCTCGTAGGA GTAGTGAAAG 601 GCCAGACAAG CCCCCCAGTC GTGGCCCACA AAGATGATTT TCTTTGGAAG GTTCAGCAGC 661 TCGAACCAAG CGGTGAGGTA CTTGTAGTGA TCCAGGAGGC GATATGAGCC ATTCCCGCTC 721 TTGCCGGACT TACCCATTCC GATCAGATCA GGGATGATGC ATCTAGCCAC GGGCTCGATG 781 TGAGGCACGA CGTGCCTCCA CAGGTAGCTG GAGGCAGCGT TACCATGCAG AAAAATCACG 841 GCGTTCTCGG CGTGCTTCTC GGAATCATAG TAGTTGATGA AGGAGTCCAG CACGTTCATT 901 TGCTTGCAGC GAGCCCACCA CTGAGGCCCA GTGATCATGC GTTTGCGTTG CTCGGGGTCG 961 TACACCTTGT AAGCCATGGT GGCTAGCCTA TAGTGAGTCG TATTAAGTAC TCTAGCCTTT 1021 AGAGCTGTAA TTGAAACTGG GAGTGGACAC TGTGGAGAGA AAGGCAAGTG GCATGTCAGT 1081 AGACATAGTG CCTATCAGAA CGCAGAGTCC TCTCTGTCTC GACAGCCAGC TTTCTATTGG 1141 ATCTCTCCTT TGAC //