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pKM2L-phSREBP2

Promoter Bank clone, Human sterol regulatory element-binding protein-2 (SREBP-2) promoter

Catalog number RDB05498
Resource name pKM2L-phSREBP2
Alternative name p1909-4F
Clone info. PCR amplified human sterol regulatory element-binding protein-2 (SREBP-2) promoter sequence was inserted into a Renilla luciferase reporter vector pKM2L (RDB04026). Cloned fragment: 41832440 to 41833270(NC_000022.11); relatively -639 to +192, where +1 corresponds to 1 nt of NM_004599.3.
Comment PCR cloning, forward primer, E5PB0037F: 5' ctggcgtggttttcaggtcctcagt 3'; reverse primer, E5PB0037R: 5' cgcccggctcagcgcagaagcc 3' Entire sequence of promoter region has not been confirmed.
Vector backbone pKM2L (RDB04026) (Plasmid)
Size of vector backbone 4.2 kb
Selectable markers Kan^r
Gene/insert name Human sterol regulatory element binding transcription factor 2 genomic DNA
Depositor|Developer DNA Bank, |
Other clones in our bank
External Database
Human sterol regulatory element binding transcription factor 2
          Reference sequence
            D86746, AL021453, AK096917
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution 1. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. 2. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
          Additional terms and conditions:
          The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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          提供条件 1. 本件リソースは学術研究機関にのみ提供し、学術研究にのみ利用することができる。　2. 謝辞の表明を必要とする。
          付加的提供条件：
          本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。
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          Catalog # Resource name Availability Shipping form Fee (non-profit org.)
          RDB05498 pKM2L-phSREBP2 Under QC test. Please contact us. DNA solution

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          How to cite this biological resource

          Materials & Methods section:

          The pKM2L-phSREBP2 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB05498).

          Reference section:

          Further references such as user reports and related articles (go to bottom)

          References and tips

          Featured content

          QC test results

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          References

          Original, user report and related articles

          user_report Marton, J., Poly(ADP-ribose) polymerase-2 is a lipid-modulated modulator of muscular lipid homeostasis. Biochim. Biophys. Acta Mol. Cell. Biol. Lipids pii: S1388-1981 (18) 30187-2 (2018). PMID 30077797.

          2023.05.02

          GNP_filter3_RDBDEP_html_230501.pl