RDB05490 - pKM2L-phGCG

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pKM2L-phGCG (#RDB05490)

Promoter Bank clone, Human glucagon promoter

Alternative name	p1898-1F
Clone info.	PCR amplified human glucagon promoter sequence was inserted into a Renilla luciferase reporter vector pKM2L (RDB04026). Cloned fragment: 162153856 to 162152194(NC_000002.12); relatively -1452 to +211, where +1 corresponds to 1 nt of NM_002054.4.
Comment	PCR cloning, forward primer, 1898F-SpeI: 5' gactactagtctagaccgacctaaggcttgacacc 3'; reverse primer, 1898R-SalI: 5' gactgtcgacagagagcaagccctctttgggaac 3'; Entire sequence of promoter region has not been confirmed. Promoter activity has not been analyzed.
Vector backbone	pKM2L (RDB04026) (Plasmid)
Size of vector backbone	4.2 kb
Selectable markers	Kan^r
Gene/insert name	Human glucagon genomic DNA
Depositor\|Developer	DNA Bank, \|

Distribution information

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Terms and conditions for distribution 1. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. 2. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
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The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.

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Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	1. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. 2. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. Additional terms and conditions: The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件	1. 本件リソースは学術研究機関にのみ提供し、学術研究にのみ利用することができる。　2. 謝辞の表明を必要とする。 MTAに書く付加的使用条件：本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB05490	pKM2L-phGCG	DNA solution	JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pKM2L-phGCG was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB05490).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB05490_A6A7p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: M13_-40 Sequence file: RDB05490_A6A7a.seq
>05490_05490_A6A7_2_M13(-40)_B10_04.ab1 1 CGGGGGTCAG CTTGCATGCC TGCAGGTCAT TTAATAGGGA TAACAGGGTA ATGATTAAGA 61 TCTCGAGCTC TAAGCTTCAC ATATGCATGC ACTAGTCTAG ACCGACCTAA GGCTTGACAC 121 CTTTCTCTGC TCTTTCACCC CCTCTGCTGG TAGAGTGCCA AGAAGCAGGT CTCAGATCAA 181 GACACCAGCA GTACCAATGA GGAGATAAAG TGAGGCAGAT ATTTTATGCA GCTGTGCTTC 241 TCAAATTTTA ATATGAACAC AGATTTCCTT AGGATTTTGT TAAAATACAG ATTCTGATAG 301 GCACGAGATT CTGCATTTCT AGCAAGCTCC CAGATGATGC TGGTCCTTAG ACCACACTTT 361 GCAAAGCAAG GCTTTCTAGC ACCCAATAAT CACCAGCACA AAAACCCCTC AATGATGTCT 421 TCCAGTCTGA CTTGAAAGTG ATCTCTGTGG CCTTCAAGTA GCTCTACATT CAAACGTTGG 481 TCTCATGCAC AAATTGAACA GTTAAGTTCA TTTGGAATAT TTCTCCAGTT GCCTCAATGA 541 TTTACCTCCA TACAAGTCAT CAGTGACATC ATGCGGTTGG TTATATTAGA ATATTGGAAT 601 CTAGTTAGGA AATGTTGTGA TGAGTTTGCA ATATGGAATA AAATTTTTAT TTTAGAAAAG 661 ACATTTACCA ACCCCTCTCA TTTTACAAAT AAGGAGGCAG AGACACAAAG AGAATGCGTG 721 ACTTGACCAA GGTCACACAG CTGGTCAATA ACAGCAAAAC TAAAATCAAA TTATGCCAAC 781 ACTACTTCTT ATGCTGTCTT TCCAGATTAT TTACCATTTT TCTATCTCTC TACAGTGACC 841 AGAATCATTT CTGCTAAATG TCACAGATCA TTAAAGCCTG TGTGTCCAGT CACAAAACTC 901 AGGAAACGTG AAAATATGCA TCTCATCTCA ACAGTTTTTC CTCATATCTC ATTCTTTTGT 961 AAACTTAGTA CCCCCACTCT CTTATCAGTA AAAATTTAGA TTTTTTAAAT ATATATTAGA 1021 ATGGAAAAAA ATACCCAGAT CATTTCCTGG CTAGCATTCA GCTATTCTTG GCATGCTTTT 1081 AATTCCATCG A //
Primer: phRLR2 Sequence file: RDB05490_A6A7b.seq
>05490_05490_A6A7_2_phRLR2_E10_13.ab1 1 ATCCAGACTC GAAGCCCAGT TTCTATTGGT CTCCTTAACC TGTCTTGTAA CCTTGATACT 61 TACCTGCCCA GTGCCTCACG ACCAACTTCT GCAGTACCCG GGTGAATTCT ACGCGTCGAC 121 AGAGAGCAAG CCCTCTTTGG GAACTTTTGA TGTGCTCTGT GTCCTAAGCT CTGTACTTCT 181 GCACCAAGGC GCACTGCTTT TATATACTCT CCAGCTCTGT TTAGCCTCAC AGATATTACG 241 CTGACAATAT AAATTTCTCA TCTGTAAATA ATGAGTTTTT GTTACAAATG ACTTCCCTCT 301 CCCACTCACT TTGCTCATCT GCATTCTATG CTTATATTGG GTGAGAGACC TTAGAGACCT 361 GGAAACTTTT TATGCACTCT TGTATCTTTT TTTTTTTTTG GTTGGGAATG GAGAGAGCAG 421 CTTGGAAATC CACCTTCTAC TTTCAATCAC GCATGAAAAT CACTTAAGTT CTCTAAAGTG 481 AATTTTGACG TTGAAGACAA AAGACCTTGA AATTACGTAG CCCATGGCCA TCCCTGTTTT 541 GTGCATGTCC TCATTTGAGG TTCTTACAAG TCTTTGGTTT GCTACCTAGC CATGATGAAT 601 TTACCTGAAT ATAACAATCA CTTCAGTCAT TCATTTATCT AAATAATGAT CATTCATTCA 661 ACACATATGT ATTAAAAATC CTCTCTTCCT CACAACGTGG AGTTTATTTA TGTTATGTTT 721 GTGGAAAATT TAAGGATATT GATTAAACCC CTATGTGTTC TGTTTTAATA AATGGAATTC 781 AGGCAGAAAG GATGGAGCAA AATGAAGATT AAACATCCAA GATAGCTGAT GCTAACAGGA 841 AATTATCTGG TATTTTTTTC CTTCTAATAT ATATTTAAAA TCTAATTTTA CTGATAAGAG 901 AGTGGGGTAC TAAGTTACAA AAGAATGAGA TATGAGGAAA ACTGTTTGAG ATGAGATGCA 961 TATTTTTCAC GTTTCCCTGA GTTTTGTGAC TGGACACACA GGCTTTAAAT GATCTGTGAC 1021 ATTTAGCCAG AAATTGAATC CTGGTTCACT GTAGAGCATA GCAACAATGC TCAATAAGTC 1081 TGGCAAAAGG ACCAGGCATT AAG //
Primer: pAxCALNL_F1 Sequence file: RDB05490_A6A7c.seq
>05490_05490_A6A7_2_pAxCALNL_F1_H10_22.ab1 1 TAGGTATCTT ATCATGTCTG CTCGAGCGGC CGCTCTAGAA TTACTGCTCG TTCTTCAGCA 61 CGCGCTCCAC GAAGCTCTTG ATGTACTTAC CCATTTCATC TGGAGCGTCC TCCTGGCTGA 121 AGTGGAGGCC CTTCACCTTC ACGAACTCGG TGTTAGGGAA CTTCTTAGCT CCCTCGACAA 181 TAGCGTTGGA AAAGAACCCA GGGTCGGACT CGATGAACAT CTTAGGCAGA TCGTCGCTGG 241 CCCGAAGGTA GGCGTTGTAG TTGCGGACAA TCTGGACGAC GTCGGGCTTG CCTCCCTTAA 301 CGAGAGGGAT CTCGCGAGGC CAGGAGAGGG TAGGCCGTCT AACCTCGCCC TTCTCCTTGA 361 ATGGCTCCAG GTAGGCAGCG AACTCCTCAG GCTCCAGTTT CCGCATGATC TTGCTTGGGA 421 GCATGGTCTC GACGAAGAAG TTATTCTCAA GCACCATTTT CTCGCCCTCT TCGCTCTTGA 481 TCAGGGCGAT ATCCTCCTCG ATGTCAGGCC ACTCGTCCCA GGACTCGATC ACGTCCACGA 541 CACTCTCAGC ATGGACGATG GCCTTGATCT TGTCTTGGTG CTCGTAGGAG TAGTGAAAGG 601 CCAGACAAGC CCCCCAGTCG TGGCCCACAA AGATGATTTT CTTTGGAAGG TTCAGCAGCT 661 CGAACCAAGC GGTGAGGTAC TTGTAGTGAT CCAGGAGGCG ATATGAGCCA TTCCCGCTCT 721 TGCCGGACTT ACCCATTCCG ATCAGATCAG GGATGATGCA TCTAGCCACG GGCTCGATGT 781 GAGGCACGAC GTGCCTCCAC AGGTAGCTGG AGGCAGCGTT ACCATGCAGA AAAAATCACG 841 GCGTTCTCGG CGTGCTTCTC GGAATCATAG TAGTTGATGA AGGAGTCCAG CACGTTCATT 901 TGCTTGCAGC GAGCCCACCC ACTGAGGCCC AGTTGATCAT GCGTTTGCGT TTGCTCGGGG 961 GTTCGGTACA CCTTTGCAAG CCCATGGTGG CCTTAGCTAT AGTGAGTCGT AATAGGTACT 1021 CTAGCCCTTT AGGAGCTGTA ATGAACTTGG TAGTGACACC T //