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pKM2L-phCYCA1 (#RDB05482)

Promoter Bank clone, Human cyclin A1 promoter

Alternative name p1890-58F
Clone info. PCR amplified human cyclin A1 promoter sequence was inserted into a Renilla luciferase reporter vector pKM2L (RDB04026). Cloned fragment: 36431252 to 36432530(NC_000013.11); relatively -1020 to +259, where +1 corresponds to 1 nt of NM_003914.3.
Comment PCR cloning, forward primer, 1890 F1: 5' cagactatagcattggtaatgtgtg 3'; reverse primer, 1890 R1: 5' ttcccgtcgttatctttctatgtg 3' Entire sequence of promoter region has not been confirmed.
Vector backbone pKM2L (RDB04026) (Plasmid)
Size of vector backbone 4.2 kb
Selectable markers Kan^r
Gene/insert name Human cyclin A1 genomic DNA
Depositor|Developer DNA Bank, |
 

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Terms and conditions for distribution 1. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. 2. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
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The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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RDB05482 pKM2L-phCYCA1 DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pKM2L-phCYCA1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB05482).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB05482_A3L9.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer:
Sequence file: RDB05482_A3L9a.seq check
>05482_05482-A3L9_3_M13(-40)_E09_15.ab1
    1 CGGCGGGCCA GCTTGCATGC CTGCAGGTCA TTTAAATAGG GATAACAGGG TAATGATTAA
   61 GATCTCGAGC TCTAAGCTTC ACATATGCAT GCACTAGTGG CCAGACTATA GCATTGGTAA
  121 TGTGTGTGTG CATATATACA TATTATTTTT AAAAAAATAA AGTTCGATTA TTTCACCTGG
  181 CTTGTCAGTC ACCTATGCAG GCGTCTGAGC CCCCGGGTTT CCAGGAGCCC CCCGTATAAG
  241 GACCCCAGGG ACTCCTCTCC CCACGCGGCC GGGCCGCCCG CCCGGCCCCC AGCCCGGAGA
  301 GCTGCCACCG ACCCCCTCAA CGTCCCAAGC CCCAGCTCTG TCGCCCGCGT TCCTTCCTCT
  361 TCCTGGGCCA CAATCTTGGC TTTCCCGGGC CGGCTTCACG CAGTTGCGCA GGAGCCCGCG
  421 GGGGAAGACC TCTCGTGGGG ACCTCGAGCA CCACGTGCGA CCCTAAATCC CCACATCTCC
  481 TCTGCCGCCT CGCAGGCCAC ATGCACCGGG AGCCGGGCGG GGCAGGCGCG GCCCGCAAGG
  541 ACCCCCGCGA TGGAGACGCA ACACTGCCGC GACTGCACTT GGGGCAGCCC CGCCGCGTCC
  601 CAGCCGCCTC CCGGCAGGAA GCGTAGGTGT GTGAGCCGAC CCGGAGCGAG CCGCGCCCTC
661 GGGCCAGCGT GGGC
//
Primer:
Sequence file: RDB05482_A3L9b.seq check
>05482_05482-A3L9_3_phRLR2_F09_18.ab1
    1 ACTCCCCTGT CTCGACAGCC CAGTTTCTAT TGGTCTCCTT AAACCTGTCT TGTAACCTTG
   61 ATACTTACCT GCCCAGTGCC TCACGACCAA CTTCTGCAGT ACCCGGGTGA ATTCTACGCG
  121 TCGACAGGCT TCCCGTCGTT ATCTTTCTAT GTGTCCGGAA CAACTGGCAA GTGCACTGGA
  181 GGATCGCGGT TAAGAGGTGG CCGGCTGGGC GGGGCAGGGC GGGGCAGGGT TGGGCAGGGC
  241 CGGGCCGGGC CGGGCGGGGC AGGGCGGGGA AGGGCAGGGA AGGGCAGGGC ACGGAAGGGC
  301 GGGGCGGGAA GGACCAAGTG TCGAGGGATT CCAGCCTGGG AGGCGGCCGG GCTTAGCGAT
  361 GCGGCTGAGC TGCCCACCCG CGTCTGTTGA ATCGGCCAAT CAGCGGCGCC CCCGGACCTA
  421 GCGGGAGCGT TTCCATGCCG ACCGCGGCAG GCGTGCGCTC TCCGGGGCCG CATCGCCATG
  481 GCGACCTGCT CACCTGACTC GCTCCGGGTG GACTCGCCGC GACCCCTGGG CGTTCAGGCC
  541 CCTGAGGGCC CAACGGGCGA CGGCCCTCCA GCTCCAGCTG TGCGTCTCCG CCCGGGCGAG
  601 GTGGGGCTGA CCGCGGCCGT CTGCACACGT CCCCTCTGGT CCGCGCCCCC GGCCTGGGAG
661 GGCGGACTCG CC
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles