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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pKM2L-phCYCA1

Promoter Bank clone, Human cyclin A1 promoter

Catalog number RDB05482
Resource name pKM2L-phCYCA1
Alternative name p1890-58F
Clone info. PCR amplified human cyclin A1 promoter sequence was inserted into a Renilla luciferase reporter vector pKM2L (RDB04026). Cloned fragment: 36431252 to 36432530(NC_000013.11); relatively -1020 to +259, where +1 corresponds to 1 nt of NM_003914.3.
Comment PCR cloning, forward primer, 1890 F1: 5' cagactatagcattggtaatgtgtg 3'; reverse primer, 1890 R1: 5' ttcccgtcgttatctttctatgtg 3' Entire sequence of promoter region has not been confirmed.
Vector backbone pKM2L (RDB04026) (Plasmid)
Size of vector backbone 4.2 kb
Selectable markers Kan^r
Gene/insert name Human cyclin A1 genomic DNA
Depositor|Developer DNA Bank, |
Other clones in our bank
External Database
Human cyclin A1
          Reference sequence
            AF124143, AL359767

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR 1. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. 2. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
          Additional terms and conditions for distribution The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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          RDB05482 pKM2L-phCYCA1 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Promoter Collection (Renilla Luciferase) (English text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB05482_A3L9.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer:
          Sequence file: RDB05482_A3L9a.seq check
          >05482_05482-A3L9_3_M13(-40)_E09_15.ab1
              1 CGGCGGGCCA GCTTGCATGC CTGCAGGTCA TTTAAATAGG GATAACAGGG TAATGATTAA
             61 GATCTCGAGC TCTAAGCTTC ACATATGCAT GCACTAGTGG CCAGACTATA GCATTGGTAA
            121 TGTGTGTGTG CATATATACA TATTATTTTT AAAAAAATAA AGTTCGATTA TTTCACCTGG
            181 CTTGTCAGTC ACCTATGCAG GCGTCTGAGC CCCCGGGTTT CCAGGAGCCC CCCGTATAAG
            241 GACCCCAGGG ACTCCTCTCC CCACGCGGCC GGGCCGCCCG CCCGGCCCCC AGCCCGGAGA
            301 GCTGCCACCG ACCCCCTCAA CGTCCCAAGC CCCAGCTCTG TCGCCCGCGT TCCTTCCTCT
            361 TCCTGGGCCA CAATCTTGGC TTTCCCGGGC CGGCTTCACG CAGTTGCGCA GGAGCCCGCG
            421 GGGGAAGACC TCTCGTGGGG ACCTCGAGCA CCACGTGCGA CCCTAAATCC CCACATCTCC
            481 TCTGCCGCCT CGCAGGCCAC ATGCACCGGG AGCCGGGCGG GGCAGGCGCG GCCCGCAAGG
            541 ACCCCCGCGA TGGAGACGCA ACACTGCCGC GACTGCACTT GGGGCAGCCC CGCCGCGTCC
            601 CAGCCGCCTC CCGGCAGGAA GCGTAGGTGT GTGAGCCGAC CCGGAGCGAG CCGCGCCCTC
          661 GGGCCAGCGT GGGC
          //
          Primer:
          Sequence file: RDB05482_A3L9b.seq check
          >05482_05482-A3L9_3_phRLR2_F09_18.ab1
              1 ACTCCCCTGT CTCGACAGCC CAGTTTCTAT TGGTCTCCTT AAACCTGTCT TGTAACCTTG
             61 ATACTTACCT GCCCAGTGCC TCACGACCAA CTTCTGCAGT ACCCGGGTGA ATTCTACGCG
            121 TCGACAGGCT TCCCGTCGTT ATCTTTCTAT GTGTCCGGAA CAACTGGCAA GTGCACTGGA
            181 GGATCGCGGT TAAGAGGTGG CCGGCTGGGC GGGGCAGGGC GGGGCAGGGT TGGGCAGGGC
            241 CGGGCCGGGC CGGGCGGGGC AGGGCGGGGA AGGGCAGGGA AGGGCAGGGC ACGGAAGGGC
            301 GGGGCGGGAA GGACCAAGTG TCGAGGGATT CCAGCCTGGG AGGCGGCCGG GCTTAGCGAT
            361 GCGGCTGAGC TGCCCACCCG CGTCTGTTGA ATCGGCCAAT CAGCGGCGCC CCCGGACCTA
            421 GCGGGAGCGT TTCCATGCCG ACCGCGGCAG GCGTGCGCTC TCCGGGGCCG CATCGCCATG
            481 GCGACCTGCT CACCTGACTC GCTCCGGGTG GACTCGCCGC GACCCCTGGG CGTTCAGGCC
            541 CCTGAGGGCC CAACGGGCGA CGGCCCTCCA GCTCCAGCTG TGCGTCTCCG CCCGGGCGAG
            601 GTGGGGCTGA CCGCGGCCGT CTGCACACGT CCCCTCTGGT CCGCGCCCCC GGCCTGGGAG
          661 GGCGGACTCG CC
          //

          Please visit Sequencing and PCR primers for primer information.

          References

          Original, user report and related articles

          2022.05.18

          GNP_filter3_RDBDEP_html_220516.pl