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pKM2L-phCXCR4 (#RDB05481)

Promoter Bank clone, Human chemokine receptor (CXCR4) promoter

Alternative name p1889-18F
Clone info. PCR amplified human chemokine receptor (CXCR4) promoter sequence was inserted into a Renilla luciferase reporter vector pKM2L (RDB04026). Cloned fragment: 136120778 to 136118108(NC_000002.12); relatively -2623 to +48, where +1 corresponds to 1 nt of NM_003467.2.
Comment PCR cloning, forward primer, 1889F-SpeI: 5' gactactagtcaattctgaatcctgccttttgcac 3'; reverse primer, 1889R-SalI: 5' gactgtcgacctcggcgtcactttgctacctg 3'. Entire sequence of promoter region has not been confirmed. Known differences (at least) : T insertion between 136,120,598 and 136,120,599 of NC_000002.12 ; C to T substitution at 136,120,697 of NC_000002.12.
Vector backbone pKM2L (RDB04026) (Plasmid)
Size of vector backbone 4.2 kb
Selectable markers Kan^r
Gene/insert name Human chemokine receptor (CXCR4) genomic DNA
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RDB05481 pKM2L-phCXCR4 DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pKM2L-phCXCR4 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB05481).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB17940_B0B3p1-1 check

Nucleotide sequence of a portion of this resource (if available).

Primer: pUC-M13_-100 (Pr0310)
Region: MCS, insert 5'
Sequence file: RDB17940_B0B3a.seq check
>05481_17940_B0B3_pUC-M13_-100_A04_01_ABI24.ab1
    1 NNNNNNNNNN NNNNNGGGNA NNGCNNGGNT TTTCCNNGTC NCGACGTTGT AAAACGACGG
   61 CCAGTGCCNA GCTTGCATGC CTGCAGGTCA TTTAAATAGG GATAACAGGG TAATGATTAA
  121 GATCTCGAGC TCTAAGCTTC ACATATGCAT GCACTAGTCA ATTCTGAATC CTGCCTTTTG
  181 CACTTAATGT TTCATAAGTA TTTCCCCATG TCACTAAAAA TTCTTCACAA ATAACATTCA
  241 CGATGTCCAT ATGGAATTTC AGATGTGGAT GAACCAAAAT CTTGTCAACT ATTCCACTAA
  301 CAGTGGTTAT TTAGGGATGT TCAGACATTT CACTATTTAA AAAAAAAATG TTTCCACAAA
  361 TACCTTTGTG GCATAAGTTT TTATGAGTGG AGTTACTGTT CTGAAGTTCC TGCTGAATAG
  421 AAAATGCTTT CCAGTGAGGC TGTCCCAAGC CACATTCCCA TCAGTGACAA GCGAGAGACA
  481 GCTGGTCTTT TCAAATCCGG AGACCAAATA TTATCTTTGA AAAAAAATGG ATTTTTGCCT
  541 AATTTGGTAG TCACCAAATA GCATCTCATT GTTCTTTTAA TTATCTGCTT CCTTTTAGTA
  601 GAGATCCCTA AAAAGATCTG AAAGGAGTCT TCAGATAAAG GAAGGAGCTT TCTTTTGTCT
  661 GTCTACAATC AACAAATATT TATTATGCAA ACCATTTTGC TCCGAGTTTT CTCCTCTTTC
  721 CCTTTTTGGA CAGATTTGGG AGATCTCACC TTTCAGGTTT TAGACATCGT GCAGGGAGGA
  781 GTTTTGAGGT AGGGTGCAGC TTACGGTCCA GGATAAAACA TACTGATTCT GCCACTACCA
  841 GGCTTTGTGA AAAGCAAGTC ATGAAAACGC TCTGAAATTC TAGANCTTCA GTAGATAGGA
  901 NCTANCGNGT CTATAAAAAT ATGAANATCC TTAAGTTTTA TTAAANATTC NAAAAAAGTA
  961 AAAGTGNTTT TACGGNTTTA TTTTCNNTTT NANTTCTTAN NNNNNNNGTT TANTNNAAAG
1021 GANNNNNNNN NGNANACNNN NNANANNNNN NNNNN
//
Primer: hRluc_N_rev (Pr0567)
Region: hRluc, intron, insert 3'
Sequence file: RDB17940_B0B3b.seq check
>05481_17940_B0B3_hRluc_N_rev_B04_04_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNCTNNNNN NNNNNNCNNC CACTGNNGCC CNGTGATCAT
   61 GCGTTTGCGT TGCTCGGGGT CGTACACCTT GGAAGCCATG GTGGCTAGCC TATAGTGAGT
  121 CGTATTAAGT ACTCTAGCCT TAAGAGCTGT AATTGAACTG GGAGTGGACA CCTGTGGAGA
  181 GAAAGGCAAA GTGGATGTCA GTAAGACCAA TAGGTGCCTA TCAGAAACGC AAGAGTCTTC
  241 TCTGTCTCGA CAAGCCCAGT TTCTATTGGT CTCCTTAAAC CTGTCTTGTA ACCTTGATAC
  301 TTACCTGCCC AGTGCCTCAC GACCAACTTC TGCAGTACCC GGGTGAATTC TACGCGTCGA
  361 CCTCGGCGTC ACTTTGCTAC CTGCTGCCGC AGCCAACAAA CTGAAGTTTC TGGCCGCGGC
  421 CGGACTTTTA TAAAAACACG CTCCGAGCGC GGCGCATGCG CCGCTGGGGC GGGGAGGGAG
  481 AAGGCGGGGT GGGGGAGAGG GGCAGACGCG AGGAAGGAGG GCGCAGGGGC GGGGCGGAGA
  541 GGTGCGCGGC TTGGGAAGCC CAGGGGACCC TGCTGTTTGC GGGTGGTCGG TAGTGAGTCC
  601 GGAGCCGGAC AGGACCTCCC AGAGGCATTT CCTAAGTTTG AGGGAAGCGG GATGCGCCTG
  661 AAGACAGGTG GGAAGCGCGG GCTAGGAACG CGTCTCTCTG TGGCGCGTCC ATCCTTGCTA
  721 AAGTGGATGG AATTCCAGAC CTGGGAATGC TACAGTAGAG ACACTGAGGC CCAGGGAANG
  781 GGAGTGACTT GCTGGAGTAA CCCAACCNGT ANGCGGCAAT GCTGGAAGTA NGGNCAAGCT
  841 TCCNACGCCA TTCCCCGCGC TTTCAGCAGA NGCAGCCCAT TCAGGAGGTA AAAGGAGTNA
  901 AGATCCTTCC TTCNGANGAN GTAGCTATAC TTGCNCATTC NCAGCATCNC TNGGGNCNNN
  961 NGCNGATAAT TANGAGANAC TTTCAGAANA ANGNNGNANT TGNNNTNNTT CNNGNANNNT
 1021 GNTCANNNGN ANNTATGTAN NGNNNNNNNA TTTCATNNNA NCNGNNNNNC NNNNTNCNNN
1081 NNNNNNN
//
Primer: pAxCALNLF1 (Pr0166)
Region: hRluc
Sequence file: RDB17940_B0B3c.seq check
>05481_17940_B0B3_pAxCALNLF1_C04_07_ABI24.ab1
    1 NNNNNNNNNN NNNNNNGNNN NNTCGNNNCG GCCGCTCTAG AATTACTGCT CGTTCTTCAG
   61 CACGCGCTCC ACGAAGCTCT TGATGTACTT ACCCATTTCA TCTGGAGCGT CCTCCTGGCT
  121 GAAGTGGAGG CCCTTCACCT TCACGAACTC GGTGTTAGGG AACTTCTTAG CTCCCTCGAC
  181 AATAGCGTTG GAAAAGAACC CAGGGTCGGA CTCGATGAAC ATCTTAGGCA GATCGTCGCT
  241 GGCCCGAAGG TAGGCGTTGT AGTTGCGGAC AATCTGGACG ACGTCGGGCT TGCCTCCCTT
  301 AACGAGAGGG ATCTCGCGAG GCCAGGAGAG GGTAGGCCGT CTAACCTCGC CCTTCTCCTT
  361 GAATGGCTCC AGGTAGGCAG CGAACTCCTC AGGCTCCAGT TTCCGCATGA TCTTGCTTGG
  421 GAGCATGGTC TCGACGAAGA AGTTATTCTC AAGCACCATT TTCTCGCCCT CTTCGCTCTT
  481 GATCAGGGCG ATATCCTCCT CGATGTCAGG CCACTCGTCC CAGGACTCGA TCACGTCCAC
  541 GACACTCTCA GCATGGACGA TGGCCTTGAT CTTGTCTTGG TGCTCGTAGG AGTAGTGAAA
  601 GGCCAGACAA GCCCCCCAGT CGTGGCCCAC AAAGATGATT TTCTTTGGAA GGTTCAGCAG
  661 CTCGAACCAA GCGGTGAGGT ACTTGTAGTG ATCCAGGAGG CGATATGAGC CATTCCCGCT
  721 CTTGCCGGAC TTACCCATTC CGATCAGATC AGGGATGATG CATCTAGCCA CGGGCTCGAT
  781 GTGAGGCACG ACGTGCCTCC ACAGGTAGCT GGAGGCAGCG TTACCATGCA GAAAAATCAC
  841 GGCGTTCTCG GCGTGCTTCT CGGAATCATA GTAGTTGATG AAGGANTCCA GCACGTTCNT
  901 TTGCTTGCAG CGAGCCCACC ACNGNNGNCC AGNGATCNTG CGTTTGCGTN GCTCGGGGNC
961 NTACNNCTTG GNANGCCNNN NNNNNTANNN NATAGNGANN CGTANNNNNN NNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles