Resource data sheet
DNA Bank Top
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pKM2L-phMMP1 (#RDB05478)

Promoter Bank clone, Human collagenase-1 (MMP-1) promoter

Alternative name p1886-2F
Clone info. PCR amplified human collagenase-1 (MMP-1) promoter sequence was inserted into a Renilla luciferase reporter vector pKM2L (RDB04026). Cloned fragment: 102800490 to 102798158 (NC_000011.10); relatively -2255 to +78, where +1 corresponds to 1nt of NM_002421.3.
Comment PCR cloning, forward primer, 1886 F1: 5' gtttccaatccacgtcagcaact 3'; reverse primer, F2PB0014R: 5' tatcccagctaggaagctccctctg 3' Known differences with NC_000011.10: A to G substitution at 102,798,479 and T to C substitution at 102,800,447. Entire sequence of promoter region has not been confirmed.
Vector backbone pKM2L (RDB04026) (Plasmid)
Size of vector backbone 4.2 kb
Selectable markers Kan^r
Gene/insert name Human collagenase-1 (MMP-1) genomic DNA
Depositor|Developer DNA Bank, |
 
Sequence RDB05478z.seq

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution 1. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. 2. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Additional terms and conditions:
The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 1. 本件リソースは学術研究機関にのみ提供し、学術研究にのみ利用することができる。　2. 謝辞の表明を必要とする。
MTAに書く付加的使用条件：
本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB05478 pKM2L-phMMP1 DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pKM2L-phMMP1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB05478).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB17373_A9Fap1-2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: M13_-40 ()
Sequence file: RDB17373_A9Faa.seq check
>05478_17373_A9Fa_1_M13_-40_E07_05_ABI08.ab1
    1 AGCCCAGGGG CAAGCTTGCA TGCCTGCAGG TCATTTAAAT AGGGATAACA GGGTAATGAT
   61 TAAGATCTCG AGCTCTAAGC TTCACATATG CATGCACTAG TGGCGTTTCC AATCCACGTC
  121 AGCAACTATG ACATTTAATG AAACACTGTG AGCATTTAGC ATGAGAGCTC TGGACTCAGA
  181 TGCAGGGAGC TTTGCTAGAG AAGGGAGGAA AAAGCAGGCA TGATGTGGCG GGTTGTGGGG
  241 GACTCCAAGG CTCTATTTCC AACTTCCATC AGAGAACTTC TGTTTTCACC TGGTTTTCAA
  301 ATTTGCTTTC CAAAAGGGAT TTTGTTTAAG TAAAGGATAC AGAGGTTTAT AAAAGTTTGA
  361 AAACTTCTAC ATTGCAGGAT GTGCAGGCTC TTGCCAGATG GGACAGTGTA TGAGACTCTT
  421 CCAGGGTGAC GTCTTAGGCA ATTTCCTGTC CAATCACAGA TGGTCACATG CTGCTTTCCT
  481 GAGTTAACCT ATTAACTCAC CCTTGTTTCC CAGGCCTCAG TGGAGCTAGG CTTGTCACGT
  541 CTTCACAGTG ACTAGATTCC CTCACAGTCG AGTATATCTG CCACTCCTTG ACTTTTAAAA
  601 CATAGTCTAT GTTCACCCTC TAATATGAAG AGCCCCTTTC ACTATTTTCT TTGTCTGTGC
  661 TGGAATCACT TCCAGGGCAA GTTGTCCTTG GTCTTCGCCC CACCCTCCCT CTGAATGCTC
  721 TGAGAAGAAG AATTCCTTTT CCTTAAAAAG GCTTCCCCAT CCTCCTACCC TCCTGAACTC
  781 ACATGGTATG CCACTTAAAT GAAGAAATTG GAGTTAAATA ATTAGAAAAG ATATGAATTA
  841 ACTCCAATCC ATTCAAGATA TTCTTAAGTA TTGTTTATGA ATTAGATATC CGTCCTGACG
  901 CCGAAAGAAA ACCAGGATTC ATAAGGGGAA GAAAGTGGTT GAAAGCAACC CGATACGTGG
  961 GGAAAGGGGG GAGACACCCT AAGGGCTGAA GTGATAAAAA AGGCCAGTGG TTCCTTCACG
 1021 TATTGGTTTT TCAATAAATG CTTTCCCAAG GGAAGGGGAG ATGGGCCATG AAGTAGGGGA
 1081 GCTACACGAG AGATAACCAA CCTTTCTTTA CCAAGGAATG CTACAGATAG CACTTGGTGA
 1141 CACCCTGGTT CACCATGTAC CCAGACCAAT TATAATGTGT GGGAACACTT AAGACGTTCT
1201 ACAC
//
Primer: phRLR2 ()
Sequence file: RDB17373_A9Fab.seq check
>05478_17373_A9Fa_1_phRLR2_F07_06_ABI08.ab1
    1 ATTCTTGGTC TCGAACAGCC CAGTTTCTAT TGGTCTCCTT AAACCTGTCT TGTAACCTTG
   61 ATACTTACCT GCCCAGTGCC TCACGACCAA CTTCTGCAGT ACCCGGGTGA ATTCTACGCG
  121 TCGACAGGCT ATCCCAGCTA GGAAGCTCCC TCTGTATATA TAGAGTCCTT GCCCTTCCAG
  181 AAAGCCAGAG GCTGTCTGAC TCATGCTTTA TAACATCCTC TTGATTAGCT ATGAATAGAC
  241 TAGGCAATCA TTAGAAATGG TGACTCCTAG CAGATTATTT GGAATCACTT GGTGTTGCAA
  301 TGTCATGATT AACTTCAAAC AAGATGTGTG CGAAGGAGAC ACACTCTGCC ATGTAAACAG
  361 TGGTTTTCTC CAATTCATTT CTTTCCTATT TGAGAAAGGT TCTTGTTATA TACACAGGTC
  421 AAAGAGTACT CCATGGTCTT TTTGAAAAAG GCTGGTTCTG ATGGTCATAA AGTGCTACAG
  481 GTTTCTCCAC ACACCTTGCT CCCAGGGCAG AGGGTGGAAT TACTAACACT GCGCACCTGA
  541 TGGCTGTTCG GCACCTGTAC TGACTGAAGT TCAGCTCCCC CAGCACTCAC TTTACGGTGG
  601 CTCTTCGGGG TTCTCTGAGG TTCCCTTCTG CCTTTGTTAA GCTGCCTGGT ACCCTATTGC
  661 GATAGCACCA TGGCTTCCCT TAATCTTTAC TCAGAGCCAG GCATGGTGGC TCAAGCCTAT
  721 AATCCCAGAA TTTCAGGAGG CCGAGGCAGG GGAATCACTT GAGCCCAGGA GTTCAAGATC
  781 AGCCTGGGCA ACATGGTGAG ACCCCATCTC TGTAAAAAAC AAACAAACAA ACAAACATTT
  841 TTTAAAGTTA GCCAAGCATG GAGTCATGCA CCTGTAGTCC TAGTCACTGA AGAGGCTGAG
  901 TGGGAGGATC ACTTGGGCCT GGGGAGGTTG AGGCTGCATT GAGCTGTGAT TGTGCCGCTG
  961 CACTCCAGCC TAGCACAGAG AGGGACCCTG TCTAAATAAA TTTGAAAAAA ATTTACTCAG
 1021 GCCTAGGATT AAGAGCCTTT ACCTGAAGAA GACCCCCTCA TCCACAAGCC GGAGTATGAG
 1081 ATACCTCCCC CACCCCCTTT GCCCATCATT ATCATGGGAC AAATAGGGAA TCTTCCTGGA
 1141 ACTGTTCTGA AATGTAACCG ATGAATGAAA AGGGCTGGAA AGGAACTGAA CGGTAAACTC
1201 TGGAGACTCG TAGAT
//
Primer: pAxCALNL_F1 ()
Sequence file: RDB17373_A9Fac.seq check
>05478_17373_A9Fa_1_pAxCALNL_F1_G07_07_ABI08.ab1
    1 CACCAGGTAT CTTATCATGT CTGCTCGAAG CGGCCGCTCT AGAATTACTG CTCGTTCTTC
   61 AGCACGCGCT CCACGAAGCT CTTGATGTAC TTACCCATTT CATCTGGAGC GTCCTCCTGG
  121 CTGAAGTGGA GGCCCTTCAC CTTCACGAAC TCGGTGTTAG GGAACTTCTT AGCTCCCTCG
  181 ACAATAGCGT TGGAAAAGAA CCCAGGGTCG GACTCGATGA ACATCTTAGG CAGATCGTCG
  241 CTGGCCCGAA GGTAGGCGTT GTAGTTGCGG ACAATCTGGA CGACGTCGGG CTTGCCTCCC
  301 TTAACGAGAG GGATCTCGCG AGGCCAGGAG AGGGTAGGCC GTCTAACCTC GCCCTTCTCC
  361 TTGAATGGCT CCAGGTAGGC AGCGAACTCC TCAGGCTCCA GTTTCCGCAT GATCTTGCTT
  421 GGGAGCATGG TCTCGACGAA GAAGTTATTC TCAAGCACCA TTTTCTCGCC CTCTTCGCTC
  481 TTGATCAGGG CGATATCCTC CTCGATGTCA GGCCACTCGT CCCAGGACTC GATCACGTCC
  541 ACGACACTCT CAGCATGGAC GATGGCCTTG ATCTTGTCTT GGTGCTCGTA GGAGTAGTGA
  601 AAGGCCAGAC AAGCCCCCCA GTCGTGGCCC ACAAAGATGA TTTTCTTTGG AAGGTTCAGC
  661 AGCTCGAACC AAGCGGTGAG GTACTTGTAG TGATCCAGGA GGCGATATGA GCCATTCCCG
  721 CTCTTGCCGG ACTTACCCAT TCCGATCAGA TCAGGGATGA TGCATCTAGC CACGGGCTCG
  781 ATGTGAGGCA CGACGTGCCT CCACAGGTAG CTGGAGGCAG CGTTACCATG CAGAAAAATC
  841 ACGGCGTTCT CGGCGTGCTT CTCGGAATCA TAGTAGTTGA TGAAGGAGTC CAGCACGTTC
  901 ATTTGCTTGC AGCGAGCCCA CCACTGAGGC CCAGTGATCA TGCGTTTGCG TTGCTCGGGG
  961 TCGTACACCT TGGAAGCCAT GGTGGCTAGC CTATAGTGAG TCGTATTAAG TACTCTAGCC
 1021 CTTAAGAGCT GTAATTGAAC TGGGAGTGGA CACCTGTGGA GGAGAAAGGC AAAGTGGATG
 1081 TCAGTTAAGA TAATAGGTGC TTATCAGAAA CGCAGAGTCT CTCTGGTTTC GACAGGCCCA
1141 GTTTCTATTG GATCTCGTTT AAACTGTGTC TTTGGTTAAC CT
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles