RDB05471 - pKM2L-phPSA(1)

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pKM2L-phPSA(1) (#RDB05471)

Promoter Bank clone, Human prostate-specific antigen/kallikrein 3 (PSA/KLK3) promoter

Alternative name	p1879-8F
Clone info.	PCR amplified human prostate-specific antigen/kallikrein 3 (PSA/KLK3) promoter sequence was inserted into a Renilla luciferase reporter vector pKM2L (RDB04026).
Comment	PCR cloning, forward primer, G1PB0007F: 5' gaattccacattgtttgctgc 3'; reverse primer, G1PB0007R: 5' tggggctggggagcctccccc 3'; Cloned fragment: 50854283 to 50854921(NC_000019.10); relatively -632 to +7, where +1 corresponds to 1 nt of NM_001648.2. Entire sequence of promoter region has not been confirmed. Known differences: C to T substitution at 50,854,560; G to A substitution at 50,854,631. Promoter activity has not been analyzed.
Vector backbone	pKM2L (RDB04026) (Plasmid)
Size of vector backbone	4.2 kb
Selectable markers	Kan^r
Gene/insert name	Human kallikrein-related peptidase 3 genomic DNA
Depositor\|Developer	DNA Bank, \|

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The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.

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Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	1. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. 2. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. Additional terms and conditions: The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.

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Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB05471	pKM2L-phPSA(1)	DNA solution	JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check

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How to cite this biological resource

Materials & Methods section:

The pKM2L-phPSA(1) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB05471).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB05471_A7B9p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: M13_-40 Sequence file: RDB05471_A7B9a.seq
>05471_05471_A7B9_M13(-40)_G05_20_ABI24.ab1 1 CAGCGTCAGC TTGCATGCCT GCAGGTCATT TAAATAGGGA TAACAGGGTA ATGATTAAGA 61 TCTCGAGCTC TAAGCTTCAC ATATGCATGC ACTAGTGGCG AATTCCACAT TGTTTGCTGC 121 ACGTTGGATT TTGAAATGCT AGGGAACTTT GGGAGACTCA TATTTCTGGG CTAGAGGATC 181 TGTGGACCAC AAGATCTTTT TATGATGACA GTAGCAATGT ATCTGTGGAG CTGGATTCTG 241 GGTTGGGAGT GCAAGGAAAA GAATGTACTA AATGCCAAGA CATCTATTTC AGGAGCATGA 301 GGAATAAAAG TTCTAGTTTC TGGTCTCAGA GTGGTGCAGG GATCAGGGAG TCTCACAATC 361 TCCTGAGTGC TGGTGTTTTA GGGCACACTG GGTCTTGGAG TGCAAAGGAT CTAGGCACGT 421 GAGGCTTTGT ATGAAGAATC GGGGATCATA CCCACCCCCT GTTTCTGTTT CATCCTGGGC 481 GTGTCTCCTC TGCCTTTGTC CCCTAGATGA AGTCTCCATG AGCTACAGGG CCTGGTGCAT 541 CCAGGGTGAT CTAGTAATTG CAGAACAGCA AGTGCTAGCT CTCCCTCCCC TTCCACAGCT 601 CTGGGTGTGG GAGGGGGTTG TCCAGCCTCC AGCAGCATGG GGAGGGCCTT GGTCAGCCTC 661 TGGGTGCCAG CAGGGCAGGG GCGGAGTCCT GGGGAATGAA GGTTTTATAG GGCTCCTGGG 721 GGAGGCTCCC CAGCCCCGCC TGTCGACGCG TAGAATTCAC CCGGGTACTG CAGAAGTTGG 781 TCGTGAGGCA CTGGGCAGGT AAGTATCAAG GTTACAAGAC AGGTTTAAGG AGACCAATAG 841 AAACTGGGCT TGTCGAGACA GAGAAGACTC TTGCGTTTCT GATAGGCACC TATTGGTCTT 901 ACTGACATCC ACTTTGCCTT TCTCTCCACA GGTGTCCACT CCCAGTTCAA TTACAGCTCT 961 TAACGCTAGA GTACTTAAAT ACGACTCACT ATAGCTAGCC ACCATGGGCT TCCCAAGTGT 1021 ACGACCCCCG AGCAACGCAA ACGCATGGAT CACTTGGTCC TTCAGTGGAT GGCTTCGCTT 1081 GCCAAGGCAA ATGAATCGGT TGCCTTGA //
Primer: phRLR2 Sequence file: RDB05471_A7B9b.seq
>05471_05471_A7B9_phRLR2_H05_23_ABI24.ab1 1 ACCTTGCCTG TCTCGACAGC CCAGTTTCTA TTGGTCTCCT TAAACCTGTC TTGTAACCTT 61 GATACTTACC TGCCCAGTGC CTCACGACCA ACTTCTGCAG TACCCGGGTG AATTCTACGC 121 GTCGACAGGC GGGGCTGGGG AGCCTCCCCC AGGAGCCCTA TAAAACCTTC ATTCCCCAGG 181 ACTCCGCCCC TGCCCTGCTG GCACCCAGAG GCTGACCAAG GCCCTCCCCA TGCTGCTGGA 241 GGCTGGACAA CCCCCTCCCA CACCCAGAGC TGTGGAAGGG GAGGGAGAGC TAGCACTTGC 301 TGTTCTGCAA TTACTAGATC ACCCTGGATG CACCAGGCCC TGTAGCTCAT GGAGACTTCA 361 TCTAGGGGAC AAAGGCAGAG GAGACACGCC CAGGATGAAA CAGAAACAGG GGGTGGGTAT 421 GATCCCCGAT TCTTCATACA AAGCCTCACG TGCCTAGATC CTTTGCACTC CAAGACCCAG 481 TGTGCCCTAA AACACCAGCA CTCAGGAGAT TGTGAGACTC CCTGATCCCT GCACCACTCT 541 GAGACCAGAA ACTAGAACTT TTATTCCTCA TGCTCCTGAA ATAGATGTCT TGGCATTTAG 601 TACATTCTTT TCCTTGCACT CCCAACCCAG AATCCAGCTC CACAGATACA TTGCTACTGT 661 CATCATAAAA AGATCTTGTG GTCCACAGAT CCTCTAGCCC AGAAATATGA GTCTCCCAAA 721 GTTCCCTAGC ATTTCAAAAT CCAACGTGCA GCAAACAATG TGGAATTCGC CACTAGTGCA 781 TGCATATGTG AAGCTTAGAG CTCGAGATCT TAATCATTAC CCTGTTATCC CTATTTAAAT 841 GACCTGCAGG CATGCAAGCT TGGCACTGGC CGTCGTTTTA CAACGTCGTG ACTGGGAAAA 901 CCCTGGCGTT ACCCAACTTA ATCGCCCTTG CAGCACATCC CCCTTTCGCC AGCTGGCGTA 961 AATAGCGAGA GGCCCGCACC CGATCGCCCT TTCCCACAGT TTGCGCAGCC CTGAAATGGC 1021 GAAATGCGAT TATTCACAAA GCCGCCGTTC CCGTCCAGGT CAGCGTAATG CTCTGCCAGT 1081 GTACGATCGA ATAAGCGAGT TCTGAATTTA GCAGAAC //
Primer: pAxCALNL_F1 Sequence file: RDB05471_A7B9c.seq
>05471_05471_A7B9_pAxCALNL_F1_A06_03_ABI24.ab1 1 ACATCTATCT TATCATGTCT GCTCGAAGCG GCCGCTCTAG AATTACTGCT CGTTCTTCAG 61 CACGCGCTCC ACGAAGCTCT TGATGTACTT ACCCATTTCA TCTGGAGCGT CCTCCTGGCT 121 GAAGTGGAGG CCCTTCACCT TCACGAACTC GGTGTTAGGG AACTTCTTAG CTCCCTCGAC 181 AATAGCGTTG GAAAAGAACC CAGGGTCGGA CTCGATGAAC ATCTTAGGCA GATCGTCGCT 241 GGCCCGAAGG TAGGCGTTGT AGTTGCGGAC AATCTGGACG ACGTCGGGCT TGCCTCCCTT 301 AACGAGAGGG ATCTCGCGAG GCCAGGAGAG GGTAGGCCGT CTAACCTCGC CCTTCTCCTT 361 GAATGGCTCC AGGTAGGCAG CGAACTCCTC AGGCTCCAGT TTCCGCATGA TCTTGCTTGG 421 GAGCATGGTC TCGACGAAGA AGTTATTCTC AAGCACCATT TTCTCGCCCT CTTCGCTCTT 481 GATCAGGGCG ATATCCTCCT CGATGTCAGG CCACTCGTCC CAGGACTCGA TCACGTCCAC 541 GACACTCTCA GCATGGACGA TGGCCTTGAT CTTGTCTTGG TGCTCGTAGG AGTAGTGAAA 601 GGCCAGACAA GCCCCCCAGT CGTGGCCCAC AAAGATGATT TTCTTTGGAA GGTTCAGCAG 661 CTCGAACCAA GCGGTGAGGT ACTTGTAGTG ATCCAGGAGG CGATATGAGC CATTCCCGCT 721 CTTGCCGGAC TTACCCATTC CGATCAGATC AGGGATGATG CATCTAGCCA CGGGCTCGAT 781 GTGAGGCACG ACGTGCCTCC ACAGGTAGCT GGAGGCAGCG TTACCATGCA GAAAAATCAC 841 GGCGTTCTCG GCGTGCTTCT CGGAATCATA GTAGTTGATG AAGGAGTCCA GCACGTTCAT 901 TTGCTTGCAG CGAGCCCACC ACTGAGGCCC AGTGATCATG CGTTTGCGTT GCTCGGGGGT 961 CGTACACCCT TGGAAGCCAT GGTGGCTAGC CTATAGTGAG TCGTATTTAA GGTACTCTAG 1021 CCCTTTAGAG CTGTAAATTG AAACTGGGAA GTTGACACCT TGTGGGAGAA GAAGGCCAAG 1081 TGCATGGTCA GGTTAGAACC CAATAGGTGT GCCTTAATCC //