Resource data sheet
Prev.

pAxCALNLwtit2

Dual cassette vector to generate recombinant adenovirus containing loxP sites and CA promoter

Catalog number RDB05214
Resource name pAxCALNLwtit2
Clone info. A dual cassette of adenovirus vector for ON-OFF regulation. Improved version of pAxCALNLw. Csp45I and PacI can be used to generate recombinant adenovirus by transfection. Conversion to recombinant adenovirus was confirmed with HEK293 cell (Nov, 2005).
The pAxCALNLwtit2 cosmid is a derivative of pAxcwit2 (RDB no. 5212) and includes a CALNL cassette [Kanegae et al., Nucleic Acids Res. 23, 3816-21, 1995; Kanegae et al., Gene 181, 207-12, 1996] at the E1 region. The pAxCALNLwtit2 cosmid, digested with Csp45I or PacI, can generate rAd upon transfection of HEK293 cells and can also be used to generate rAd by the COS-TPC method [Miyake et al., Proc. Natl. Acad. Sci. USA 93, 1320-24, 1996]. The resultant rAd expresses the gene of interest via the Cre-mediated control of gene expression by an expression cassette that provides the on/off-switching reporter unit and is composed of a CA promoter [Niwa et al., Gene 108, 193-99, 1991.], a loxP sequence, a stuffer sequence, and a second loxP sequence, which is followed by the SwaI cloning site and the G polyA sequence, namely, a CALNL cassette [Kanegae et al., Nucleic Acids Res. 23, 3816-21, 1995; Kanegae et al., Gene 181, 207-12, 1996].
Vector backbone charomid 9-11 (Cosmid, use packaging extracts for transformation)
Size of vector backbone 11 kb
Selectable markers Amp^r
Gene/insert name AdV_5 - Genomic DNA
Depositor Saito, Izumu |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Terashima, M., Exp. Med., 21, 931-936(2003)).
Ordering Please visit Information of Request for Distribution. 
Order form 
[Credit Card Payment]  [Bank Transfer or Check Payment]
Material Transfer Agreement (MTA) [Word]
Remarks ((Additional form)) CAG(FormE)
Remember that you will be working with samples containing infectious virus.
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB05214 pAxCALNLwtit2 DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file PDF RDB05214.pdf from Depositor

Featured content

Featured content Empty Backbone (English text)
Featured content Empty Backbone (Japanese text)

References

original Fukuda, H., Possible mechanism of adenovirus generation from a cloned viral genome tagged with nucleotides at its ends. Microbiol. Immunol., 50, 643-654 (2006). PMID 16924150.
original Terashima, M., Exp. Med., 21, 931-936 (2003).

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB12784_A4Dsp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pCAG-F_V2 RDB12784_A4Dsa_v2.seq
>05214_12784_A4Ds-pCAG-F_V2_A04_01.ab1
    1 GCACAGTCAA TCTGTGCGGA GCCGAATCTG GGAGGCGCCG CCGCACCCCC TCTAGCGGGC
   61 GCGGGGCGAA GCGGTGCGGC GCCGGCAGGA AGGAAATGGG CGGGGAGGGC CTTCGTGCGT
  121 CGCCGCGCCG CCGTCCCCTT CTCCCTCTCC AGCCTCGGGG CTGTCCGCGG GGGGACGGCT
  181 GCCTTCGGGG GGGACGGGGC AGGGCGGGGT TCGGCTTCTG GCGTGTGACC GGCGGCTCTA
  241 GAGCCTCTGC TAACCATGTT CATGCCTTCT TCTTTTTCCT ACAGCTCCTG GGCAACGTGC
  301 TGGTTATTGT GCTGTCTCAT CATTTTGGCA AAGAATTGAT TAATTCGAGC GAACGCGTAT
  361 AACTTCGTAT AGCATACATT ATACGAAGTT ATCTCGAGTC GGATTTGATC TGATCAAGAG
  421 ACAGGATGAG GATCGTTTCG CATGATTGAA CAAGATGGAT TGCACGCAGG TTCTCCGGCC
  481 GCTTGGGTGG AGAGGCTATT CGGCTATGAC TGGGCACAAC AGACAATCGG CTGCTCTGAT
  541 GCCGCCGTGT TCCGGCTGTC AGCGCAGGGG CGCCCGGTTC TTTTTGTCAA GACCGACCTG
  601 TCCGGTGCCC TGAATGAACT GCAGGACGAG GCAGCGCGGC TATCGTGGCT GGCCACGACG
  661 GGCGTTCCTT GCGCAGCTGT GCTCGACGTT GTCACTGAAG CGGGAAGGGA CTGGCTGCTA
  721 TTGGGCGAAG TGCCGGGGCA GGATCTCCTG TCATCTCACC TTGCTCCTGC CGAGAAAGTA
  781 TCCATCATGG CTGATGCAAT GCGGCGGCTG CATACGCTTG ATCCGGCTAC CTGCCCATTC
  841 GACCACCAAG CGAAACATCG CATCGAGCGA GCACGTACTC GGATGGAAGC CGGTCTTGTC
  901 GATCAGATGA TCTGGACGAA GAGCATCAGG GGCTCGCGCC AGCCGAACTG TTCGCCAGGC
  961 TCAAGCGCGC ATGCCGACGC GAGGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA
 1021 TATTCATGGG TGGAAGATGG CCGCTATTCC TGGAGATCCA TCGACCTGTA TGGCCGGCCT
 1081 GGGATGTATG TCGGAACGGC ATATCATGGA CATTACCGAT TTGTCGATCC ACAGTCACTA
1141 TAGCTGTGAG TAGAAGACTA TTGATAGCGA CGCA
//
Primer: Neo-C_F RDB12784_A4Dsb_v2.seq
>05214_12784_A4Ds-Neo-C_F_B04_04.ab1
    1 CGTCTGTAAT TGCTGAGAGC TTGGCGGCGA ATGGGCTGAC CGCTTCCTCG TGCTTTACGG
   61 TATCGCCGCT CCCGATTCGC AGCGCATCGC CTTCTATCGC CTTCTTGACG AGTTCTTCTG
  121 AGCGGGACTC TGGGGTTCGC GAAATGACCG ACCAAGCGAC GCCCAACCTG CCATCACGAG
  181 ATTTCGATTC CACCGCCGCC TTCTATGAAA GGTTGGGCTT CGGAATCGTT TTCCGGGACG
  241 CCGGCTGGAT GATCCTCCAG CGCGGGGATC TCATGCTGGA GTTCTTCGCC CACCCCATCG
  301 ATAACTTGTT TATTGCAGCT TATAATGGTT ACAAATAAAG CAATAGCATC ACAAATTTCA
  361 CAAATAAAGC ATTTTTTTCA CTGCATTCTA GTTGTGGTTT GTCCAAACTC ATCAATGTAT
  421 CTTATCATGT CTGGATCAAT CCGAACGCGT ATAACTTCGT ATAGCATACA TTATACGAAG
  481 TTATCTCGAG TCGCTCGGTA CGATTTCCAT CGATTTAAAT TATAAACTAG TCTAGAATCG
  541 ATGGGGATCA AATCAATTCA CTCCTCAGGT GCAGGCTGCC TATCAGAAGG TGGTGGCTGG
  601 TGTGGCCAAT GCCCTGGCTC ACAAATACCA CTGAGATCTT TTTCCCTCTG CCAAAAATTA
  661 TGGGGACATC ATGAAGCCCC TTGAGCATCT GACTTCTGGC TAATAAAGGA AATTTATTTT
  721 CATTGCAATA GTGTGTTGGA ATTTTTTGTG TCTCTCACTC GGAATGACAT ATGGGAGGGC
  781 AAATCATTTA AAACATCAGA ATGAGTATTT GGGTTTAGAG TTTGGCAACA TATGCCCATA
  841 TGCTGGCTGC CATGAACAAA GGTTGGCTAT AAAGAGGTCA TCAGTATATG AACAGCCCCT
  901 GCTGTCCATC GTATCATAGA AAAGGCTGGA CTGGAGTAAG ATTTTTTTTA ATTGGTTGGT
  961 AATTTTTCTA CATCCTAAAT TTCTTACTGT TTACTAGCGG ACTTTACTCC TCCTGACTAC
 1021 TCAGTCTAGC GTACACCTTT CGTAGATCCT GACTGAAGCC AGCTGATCAG AGTCTGGACT
1081 A
//
Primer: Amp_R RDB12784_A4Dsc_v2.seq
>05214_12784_A4Ds-Amp_R_C04_07.ab1
    1 CTCGTTTCAT ATTATTGAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG
   61 AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC
  121 CTGACGTCTA AGAAACCATT ATTATCATGA CATTAACCTA TAAAAATAGG CGTATCACGA
  181 GGCCCTTTCG TCTTCAAGAA TTCCGGATCG ATCCGCATGT TCGATTTCGA TTTAATTAAT
  241 TCGAACATCA TCAATAATAT ACCTTATTTT GGATTGAAGC CAATATGATA ATGAGGGGGT
  301 GGAGTTTGTG ACGTGGCGCG GGGCGTGGGA ACGGGGCGGG TGACGTAGTA GTGTGGCGGA
  361 AGTGTGATGT TGCAAGTGTG GCGGAACACA TGTAAGCGAC GGATGTGGCA AAAGTGACGT
  421 TTTTGGTGTG CGCCGGTGTA CACAGGAAGT GACAATTTTC GCGCGGTTTT AGGCGGATGT
  481 TGTAGTAAAT TTGGGCGTAA CCGAGTAAGA TTTGGCCATT TTCGCGGGAA AACTGAATAA
  541 GAGGAAGTGA AATCTGAATA ATTTTGTGTT ACTCATAGCG CGTAATATTT GTCTAGGGCC
  601 GCGGGGACTT TGACCGTTTA CGTGGAGACT CGCCCAGGTG TTTTTCTCAG GTGTTTTCCG
  661 CGTTCCGGGT CAAAGTTGGC GTTTTATTAT TATAGTCAGC ATCGATTTAG CTTGGGCTGC
  721 AGGTCGAGGG ATCTCCATAA GAGAAGAGGG ACAGCTATGA CTGGGAGTAG TCAGGAGAGG
  781 AGGAAAAATC TGGCTAGTAA AACATGTAAG GAAAATTTTA GGGATGTTAA AGAAAAAAAT
  841 AACACAAAAC AAAAATATAA AAAAAATCTA ACCTCAAGTC AAGGCTTTTC TATGGAATAA
  901 GAATGGACAG CAAGGGGGCT GTTTCATATA CTGATGACCT CTTTATAGCC AACCCTTTGT
  961 TCATGGGCAG CCAGCATATG GGCATATGTT TGCCAAACTC TAAACCCAAA TACTCATTCT
 1021 GATGTTTTAA TGATTTGCCC TTCCCATATG TCCTTCCGAG TGAGAAGACA CAAAAATTCC
 1081 AAACACACTA ATTGCCAATG AAATAATTGC ATTTAATAGC CAGAAAGTTC AGATGCCTTC
1141 ACAGAGGGGA CTCTCTC
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Fukuda, H., Possible mechanism of adenovirus generation from a cloned viral genome tagged with nucleotides at its ends. Microbiol. Immunol., 50, 643-654 (2006). PMID 16924150.
original Terashima, M., Exp. Med., 21, 931-936 (2003).

2018.12.10

GNP_filter3_RDBDEP_html_181012.pl