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pAxCALNLwtit2 (#RDB05214)

Dual cassette vector to generate recombinant adenovirus containing loxP sites and CA promoter


Drawn by SnapGene® software
Sequence information
(Assembled from experimentally sequenced data)		 GenBank Flat File Format open
SnapGene file download
Remarks, protocol and/or map (pdf) RDB05214.pdf
Publication Fukuda, H., Microbiol. Immunol., 50, 643-654 (2006). PMID 16924150. [PubMed] [Article] [RRC of NBRP]
Publication Terashima, M., Exp. Med., 21, 931-936 (2003).
Test sheet Data Sheet open 
 
Clone info. A dual cassette of adenovirus vector for ON-OFF regulation. Improved version of pAxCALNLw. Csp45I and PacI can be used to generate recombinant adenovirus by transfection. Conversion to recombinant adenovirus was confirmed with HEK293 cell (Nov, 2005).
Comment The pAxCALNLwtit2 cosmid is a derivative of pAxcwit2 (RDB05212) and includes a CALNL cassette [Kanegae et al., Nucleic Acids Res. 23, 3816-21, 1995; Kanegae et al., Gene 181, 207-12, 1996] at the E1 region. The pAxCALNLwtit2 cosmid, digested with Csp45I or PacI, can generate rAd upon transfection of HEK293 cells and can also be used to generate rAd by the COS-TPC method [Miyake et al., Proc. Natl. Acad. Sci. USA 93, 1320-24, 1996]. The resultant rAd expresses the gene of interest via the Cre-mediated control of gene expression by an expression cassette that provides the on/off-switching reporter unit and is composed of a CA promoter [Niwa et al., Gene 108, 193-99, 1991.], a loxP sequence, a stuffer sequence, and a second loxP sequence, which is followed by the SwaI cloning site and the G polyA sequence, namely, a CALNL cassette [Kanegae et al., Nucleic Acids Res. 23, 3816-21, 1995; Kanegae et al., Gene 181, 207-12, 1996].
Vector backbone charomid 9-11 (Cosmid, use packaging extracts for transforming E. coli host)
Size of vector backbone 11 kb
Selectable markers Amp^r
Growth conditions 37C, LB+Amp
Gene/insert name AdV_5 - Genomic DNA
Depositor|Developer Saito, Izumu |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Terashima, M., Exp. Med., 21, 931-936(2003)).
Additional terms and conditions:
Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.
Remarks Remember that you will be working with samples containing infectious virus.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Terashima, M., Exp. Med., 21, 931-936(2003))。
付加的使用条件：
CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学 宮崎純一博士への謝辞の表明を必要とする。
備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog # Resource name Shipping form Fee
RDB05214 pAxCALNLwtit2 DNA solution

How to cite this biological resource

Materials & Methods section:

The pAxCALNLwtit2 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB05214).

Reference section:

Fukuda, H., Terashima, M., Koshikawa, M., Kanegae, Y., Saito, I., Possible mechanism of adenovirus generation from a cloned viral genome tagged with nucleotides at its ends. Microbiol. Immunol., 50, 643-654 (2006). PMID 16924150. [PubMed] [Article] [RRC of NBRP]
Terashima, M., Kondo, S., Kanegae, Y., Saito, I., Exp. Med., 21, 931-936 (2003).

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Fukuda, H., Possible mechanism of adenovirus generation from a cloned viral genome tagged with nucleotides at its ends. Microbiol. Immunol., 50, 643-654 (2006). PMID 16924150. [PubMed] [Article] [RRC of NBRP]
original Terashima, M., Exp. Med., 21, 931-936 (2003).