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pAxCAwtit2

Dual cassette vector to generate recombinant adenovirus containing CA promoter

Catalog number RDB05213
Resource name pAxCAwtit2
Clone info. A dual cassette for constructing recombinant adenovirus containing CAG promoter. Improved version of pAxCAwtit. Csp45I and PacI can be used to generate recombinant adenovirus by transfection. Conversion to recombinant adenovirus was confirmed with HEK293 cell (Nov, 2005).
The pAxCAwtit2 cosmid is a derivative of pAxcwit2 (RDB no. 5212) and includes a CAG cassette [Niwa et al., Gene 108, 193-99, 1991], harboring a unique SwaI site as the cloning site for the gene of interest, at the E1 region. The pAxCAwtit2 cosmid, digested with Csp45I or PacI, can generate rAd upon transfection of HEK293 cells and can also be used to generate rAd by the COS-TPC method [Miyake et al., Proc. Natl. Acad. Sci. USA 93, 1320-24, 1996].
Vector backbone charomid 9-11 (Cosmid, use packaging extracts for transformation)
Selectable markers Amp^r
Gene/insert name AdV_5 - Genomic DNA
Depositor Saito, Izumu |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Terashima, M., Exp. Med., 21, 931-936(2003)).
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Remarks ((Additional form)) CAG(FormE)
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RDB05213 pAxCAwtit2 DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file PDF RDB05213.pdf from Depositor

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References

original Fukuda, H., Possible mechanism of adenovirus generation from a cloned viral genome tagged with nucleotides at its ends. Microbiol. Immunol., 50, 643-654 (2006). PMID 16924150.
original Terashima, M., Exp. Med., 21, 931-936 (2003).

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB13981_A6C4p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pCAG-F_V2 RDB13981_A6C4a.seq
>05213_13981_A6C4_pCAG-F_V2_A04_01.ab1
    1 GGACGTTGTC AATCTGTGCG GAGCCGAATC TGGGAGGCGC CGCCGCACCC CCTCTAGCGG
   61 GCGCGGGGCG AAGCGGTGCG GCGCCGGCAG GAAGGAAATG GGCGGGGAGG GCCTTCGTGC
  121 GTCGCCGCGC CGCCGTCCCC TTCTCCCTCT CCAGCCTCGG GGCTGTCCGC GGGGGGACGG
  181 CTGCCTTCGG GGGGGACGGG GCAGGGCGGG GTTCGGCTTC TGGCGTGTGA CCGGCGGCTC
  241 TAGAGCCTCT GCTAACCATG TTCATGCCTT CTTCTTTTTC CTACAGCTCC TGGGCAACGT
  301 GCTGGTTATT GTGCTGTCTC ATCATTTTGG CAAAGAATTG ATTTATCGAT TTAAATTATA
  361 AACTAGTCTA GAATCGATAA TCAATTCACT CCTCAGGTGC AGGCTGCCTA TCAGAAGGTG
  421 GTGGCTGGTG TGGCCAATGC CCTGGCTCAC AAATACCACT GAGATCTTTT TCCCTCTGCC
  481 AAAAATTATG GGGACATCAT GAAGCCCCTT GAGCATCTGA CTTCTGGCTA ATAAAGGAAA
  541 TTTATTTTCA TTGCAATAGT GTGTTGGAAT TTTTTGTGTC TCTCACTCGG AAGGACATAT
  601 GGGAGGGCAA ATCATTTAAA ACATCAGAAT GAGTATTTGG TTTAGAGTTT GGCAAACATA
  661 TGCCCATATG CTGGCTGCCA TGAACAAAGG TTGGCTATAA AGAGGTCATC AGTATATGAA
  721 ACAGCCCCCT GCTGTCCATT CCTTATTCCA TAGAAAAGCC TTGACTTGAG GTTAGATTTT
  781 TTTTATATTT TGTTTTGTGT TATTTTTTTC TTTAACATCC CCTAAAAATT TTCCCTTACA
  841 TGTTTTACTA GCCAGATTTT TTCCTTCCTC TCCTGACTAC TCCCCAGTCA TAGCTGTCCC
  901 CTCTTTCTCT AATGGGAGAT CCCTCGACCC CCTCGAAGGG CCCCAAGCTG GTTTAAACGA
  961 TGGCTTGACT ATAATTAATA AACGACCCAC TTTGAACCCG GACGGCGGAA AAAACACCTG
 1021 AGAAAATCAC CTGGGCGAAG TCCTTCCACG ATAAACCGGA TCAAAGATCC CCCGCGGGCC
 1081 TAGATACAGA ATAATACGCG CCTATTGGAG TTACCTCGAA GTAATCGACT TCACGTTCCG
1141 TCACCTGATC AGTTTCTCCC CAGCA
//
Primer: Amp_R RDB13981_A6C4b.seq
>05213_13981_A6C4_Amp_R_B04_04.ab1
    1 CTCGTTTCAT ATTATTGAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG
   61 AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC
  121 CTGACGTCTA AGAAACCATT ATTATCATGA CATTAACCTA TAAAAATAGG CGTATCACGA
  181 GGCCCTTTCG TCTTCAAGAA TTCCGGATCG ATCCGCATGT TCGATTTCGA TTTAATTAAT
  241 TCGAACATCA TCAATAATAT ACCTTATTTT GGATTGAAGC CAATATGATA ATGAGGGGGT
  301 GGAGTTTGTG ACGTGGCGCG GGGCGTGGGA ACGGGGCGGG TGACGTAGTA GTGTGGCGGA
  361 AGTGTGATGT TGCAAGTGTG GCGGAACACA TGTAAGCGAC GGATGTGGCA AAAGTGACGT
  421 TTTTGGTGTG CGCCGGTGTA CACAGGAAGT GACAATTTTC GCGCGGTTTT AGGCGGATGT
  481 TGTAGTAAAT TTGGGCGTAA CCGAGTAAGA TTTGGCCATT TTCGCGGGAA AACTGAATAA
  541 GAGGAAGTGA AATCTGAATA ATTTTGTGTT ACTCATAGCG CGTAATATTT GTCTAGGGCC
  601 GCGGGGACTT TGACCGTTTA CGTGGAGACT CGCCCAGGTG TTTTTCTCAG GTGTTTTCCG
  661 CGTTCCGGGT CAAAGTTGGC GTTTTATTAT TATAGTCAGC ATCGTTTAAA CAGCTTGGGC
  721 CCTCGAGGGG TCGAGGGATC TCCATAAGAG AAGAGGGACA GCTATGACTG GGAGTAGTCA
  781 GGAGAGGAGG AAAAATCTGG CTAGTAAAAC ATGTAAGGAA AATTTTAGGG ATGTTAAAGA
  841 AAAAAAATAA CACAAAACAA AATATAAAAA AAAATCTAAC CTCAAGTCAA GGCTTTTCTA
  901 TGGAATAAAG AATGGACAGC AGGGGCTGTT TCATATACTG ATGACCTCTT TATAGCCAAC
  961 CTTTGTTCAT GGCAGCCAGC ATATGGCATA TGTGCCAACT CTAACCAATA CTCAATCTGA
 1021 TGTTTAATTG ATTGGCCTCC ATATGTCCGT CCGAGTGAAG ACACTACAAA AAGTCGAACA
1081 CACTATGCAT GCACACTAAA TTGCGTTAAT TAAGCTAGAA AAGTTCACGA AATGCTAT
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Fukuda, H., Possible mechanism of adenovirus generation from a cloned viral genome tagged with nucleotides at its ends. Microbiol. Immunol., 50, 643-654 (2006). PMID 16924150.
original Terashima, M., Exp. Med., 21, 931-936 (2003).
user report Kurihara, C., An easy method for preparation of Cre-loxP regulated fluorescent adenoviral expression vectors and its application for direct reprogramming into hepatocytes. Biotechnol. Rep. (Amst.) 12: 26-32 (2016). PMID 28352551.

2018.12.10

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