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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pAxCAwtit2

Dual cassette vector to generate recombinant adenovirus containing CA promoter

Catalog number RDB05213
Resource name pAxCAwtit2
Clone info. A dual cassette for constructing recombinant adenovirus containing CAG promoter. Improved version of pAxCAwtit. Csp45I and PacI can be used to generate recombinant adenovirus by transfection. Conversion to recombinant adenovirus was confirmed with HEK293 cell (Nov, 2005).
Comment The pAxCAwtit2 cosmid is a derivative of pAxcwit2 (RDB no. 5212) and includes a CAG cassette [Niwa et al., Gene 108, 193-99, 1991], harboring a unique SwaI site as the cloning site for the gene of interest, at the E1 region. The pAxCAwtit2 cosmid, digested with Csp45I or PacI, can generate rAd upon transfection of HEK293 cells and can also be used to generate rAd by the COS-TPC method [Miyake et al., Proc. Natl. Acad. Sci. USA 93, 1320-24, 1996].
Vector backbone charomid 9-11 (Cosmid, use packaging extracts for transformation)
Selectable markers Amp^r
Gene/insert name AdV_5 - Genomic DNA
Depositor|Developer Saito, Izumu |
Other clones in our bank

External Database
AdV_5 -

            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Terashima, M., Exp. Med., 21, 931-936(2003)).
            Additional terms and conditions for distribution Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.
            Ordering Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
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            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            Remarks Remember that you will be working with samples containing infectious virus.
            提供案内 (日本国内) [open/close]

            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Terashima, M., Exp. Med., 21, 931-936(2003))。
            付加的提供条件 CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学 宮崎純一博士への謝辞の表明を必要とする。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
            備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB05213 pAxCAwtit2 DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Remarks, protocol and/or map (pdf) RDB05213.pdf

            Original reference

            original Fukuda, H., Possible mechanism of adenovirus generation from a cloned viral genome tagged with nucleotides at its ends. Microbiol. Immunol., 50, 643-654 (2006). PMID 16924150.
            original Terashima, M., Exp. Med., 21, 931-936 (2003).

            Further references such as user reports and related articles (go to bottom)

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            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB13981_A6C4p1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: pCAG-F_V2
            Sequence file: RDB13981_A6C4a.seq check
            >05213_13981_A6C4_pCAG-F_V2_A04_01.ab1
                1 GGACGTTGTC AATCTGTGCG GAGCCGAATC TGGGAGGCGC CGCCGCACCC CCTCTAGCGG
               61 GCGCGGGGCG AAGCGGTGCG GCGCCGGCAG GAAGGAAATG GGCGGGGAGG GCCTTCGTGC
              121 GTCGCCGCGC CGCCGTCCCC TTCTCCCTCT CCAGCCTCGG GGCTGTCCGC GGGGGGACGG
              181 CTGCCTTCGG GGGGGACGGG GCAGGGCGGG GTTCGGCTTC TGGCGTGTGA CCGGCGGCTC
              241 TAGAGCCTCT GCTAACCATG TTCATGCCTT CTTCTTTTTC CTACAGCTCC TGGGCAACGT
              301 GCTGGTTATT GTGCTGTCTC ATCATTTTGG CAAAGAATTG ATTTATCGAT TTAAATTATA
              361 AACTAGTCTA GAATCGATAA TCAATTCACT CCTCAGGTGC AGGCTGCCTA TCAGAAGGTG
              421 GTGGCTGGTG TGGCCAATGC CCTGGCTCAC AAATACCACT GAGATCTTTT TCCCTCTGCC
              481 AAAAATTATG GGGACATCAT GAAGCCCCTT GAGCATCTGA CTTCTGGCTA ATAAAGGAAA
              541 TTTATTTTCA TTGCAATAGT GTGTTGGAAT TTTTTGTGTC TCTCACTCGG AAGGACATAT
              601 GGGAGGGCAA ATCATTTAAA ACATCAGAAT GAGTATTTGG TTTAGAGTTT GGCAAACATA
              661 TGCCCATATG CTGGCTGCCA TGAACAAAGG TTGGCTATAA AGAGGTCATC AGTATATGAA
              721 ACAGCCCCCT GCTGTCCATT CCTTATTCCA TAGAAAAGCC TTGACTTGAG GTTAGATTTT
              781 TTTTATATTT TGTTTTGTGT TATTTTTTTC TTTAACATCC CCTAAAAATT TTCCCTTACA
              841 TGTTTTACTA GCCAGATTTT TTCCTTCCTC TCCTGACTAC TCCCCAGTCA TAGCTGTCCC
              901 CTCTTTCTCT AATGGGAGAT CCCTCGACCC CCTCGAAGGG CCCCAAGCTG GTTTAAACGA
              961 TGGCTTGACT ATAATTAATA AACGACCCAC TTTGAACCCG GACGGCGGAA AAAACACCTG
             1021 AGAAAATCAC CTGGGCGAAG TCCTTCCACG ATAAACCGGA TCAAAGATCC CCCGCGGGCC
             1081 TAGATACAGA ATAATACGCG CCTATTGGAG TTACCTCGAA GTAATCGACT TCACGTTCCG
            1141 TCACCTGATC AGTTTCTCCC CAGCA
            //
            Primer: Amp_R
            Sequence file: RDB13981_A6C4b.seq check
            >05213_13981_A6C4_Amp_R_B04_04.ab1
                1 CTCGTTTCAT ATTATTGAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG
               61 AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC
              121 CTGACGTCTA AGAAACCATT ATTATCATGA CATTAACCTA TAAAAATAGG CGTATCACGA
              181 GGCCCTTTCG TCTTCAAGAA TTCCGGATCG ATCCGCATGT TCGATTTCGA TTTAATTAAT
              241 TCGAACATCA TCAATAATAT ACCTTATTTT GGATTGAAGC CAATATGATA ATGAGGGGGT
              301 GGAGTTTGTG ACGTGGCGCG GGGCGTGGGA ACGGGGCGGG TGACGTAGTA GTGTGGCGGA
              361 AGTGTGATGT TGCAAGTGTG GCGGAACACA TGTAAGCGAC GGATGTGGCA AAAGTGACGT
              421 TTTTGGTGTG CGCCGGTGTA CACAGGAAGT GACAATTTTC GCGCGGTTTT AGGCGGATGT
              481 TGTAGTAAAT TTGGGCGTAA CCGAGTAAGA TTTGGCCATT TTCGCGGGAA AACTGAATAA
              541 GAGGAAGTGA AATCTGAATA ATTTTGTGTT ACTCATAGCG CGTAATATTT GTCTAGGGCC
              601 GCGGGGACTT TGACCGTTTA CGTGGAGACT CGCCCAGGTG TTTTTCTCAG GTGTTTTCCG
              661 CGTTCCGGGT CAAAGTTGGC GTTTTATTAT TATAGTCAGC ATCGTTTAAA CAGCTTGGGC
              721 CCTCGAGGGG TCGAGGGATC TCCATAAGAG AAGAGGGACA GCTATGACTG GGAGTAGTCA
              781 GGAGAGGAGG AAAAATCTGG CTAGTAAAAC ATGTAAGGAA AATTTTAGGG ATGTTAAAGA
              841 AAAAAAATAA CACAAAACAA AATATAAAAA AAAATCTAAC CTCAAGTCAA GGCTTTTCTA
              901 TGGAATAAAG AATGGACAGC AGGGGCTGTT TCATATACTG ATGACCTCTT TATAGCCAAC
              961 CTTTGTTCAT GGCAGCCAGC ATATGGCATA TGTGCCAACT CTAACCAATA CTCAATCTGA
             1021 TGTTTAATTG ATTGGCCTCC ATATGTCCGT CCGAGTGAAG ACACTACAAA AAGTCGAACA
            1081 CACTATGCAT GCACACTAAA TTGCGTTAAT TAAGCTAGAA AAGTTCACGA AATGCTAT
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Fukuda, H., Possible mechanism of adenovirus generation from a cloned viral genome tagged with nucleotides at its ends. Microbiol. Immunol., 50, 643-654 (2006). PMID 16924150.
            original Terashima, M., Exp. Med., 21, 931-936 (2003).
            user_report Kurihara, C., An easy method for preparation of Cre-loxP regulated fluorescent adenoviral expression vectors and its application for direct reprogramming into hepatocytes. Biotechnol. Rep. (Amst.) 12: 26-32 (2016). PMID 28352551.

            2022.05.18

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