Resource data sheet
DNA Bank Top
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pAxCALNLhSTAT3(forward) (#RDB04699)

Shuttle vector to generate rAd expressing human STAT3

Alternative name 1425C8F
Clone info. Shuttle vector to generate rAd expressing human STAT3. Cre-recombinase is required to remove stuffer sequence.
Comment Cre-recombinase is required to remove LNL stuffer sequence. Use COS-TPC method for the production of adenovirus.
Vector backbone pAxCALNLw (Cosmid, use packaging extracts for transforming E. coli host)
Size of vector backbone 46074 bp
Selectable markers Amp^r
Gene/insert name Human STAT3 cDNA
Depositor|Developer DNA Bank, |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature (Gene 200: 149-156, 1997) and acknowledgement to the DNA Bank are requested.
Additional terms and conditions:
Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.
Remarks Remember that you will be working with samples containing infectious virus.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 研究発表に際し、理研DNA Bankヘの謝辞ならびに次の論文の引用を必要とする (Gene 200: 149-156, 1997)。
MTAに書く付加的使用条件：
CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学 宮崎純一博士への謝辞の表明を必要とする。
備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB04699 pAxCALNLhSTAT3(forward) DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pAxCALNLhSTAT3(forward) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB04699).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB04699_A4Kb.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCA_F1
Sequence file: RDB04699_A4Kba.seq check
>04699_04699_A4Kb_15_pAxCA_F1_B04_04.ab1
    1 CTTCCCTGCT ACATGTTCAT GCCTTCTTCT TTTTCCTACA GCTCCTGGGC AACGTGCTGG
   61 TTATTGTGCT GTCTCATCAT TTTGGCAAAG AATTGATTAA TTCGAGCGAA CGCGTATAAC
  121 TTCGTATAGC ATACATTATA CGAAGTTATC TCGAGTCGGA TTTGATCTGA TCAAGAGACA
  181 GGATGAGGAT CGTTTCGCAT GATTGAACAA GATGGATTGC ACGCAGGTTC TCCGGCCGCT
  241 TGGGTGGAGA GGCTATTCGG CTATGACTGG GCACAACAGA CAATCGGCTG CTCTGATGCC
  301 GCCGTGTTCC GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT TTGTCAAGAC CGACCTGTCC
  361 GGTGCCCTGA ATGAACTGCA GGACGAGGCA GCGCGGCTAT CGTGGCTGGC CACGACGGGC
  421 GTTCCTTGCG CAGCTGTGCT CGACGTTGTC ACTGAAGCGG GAAGGGACTG GCTGCTATTG
  481 GGCGAAGTGC CGGGGCAGGA TCTCCTGTCA TCTCACCTTG CTCCTGCCGA GAAAGTATCC
  541 ATCATGGCTG ATGCAATGCG GCGGCTGCAT ACGCTTGATC CGGCTACCTG CCCATTCGAC
  601 CACCAAGCGA AACATCGCAT CGAGCGAGCA CGTACTCGGA TGGAAGCCGG TCTTGTCGAT
  661 CAGGATGATC TGGACGAAGA GCATCAGGGG CTCGCGCCAG CCGAACTGTT CGCCAGGCTC
  721 AAGGCGCGCA TGCCCGACGG CGAGGATCTC GTCGTGACCC ATGGCGATGC CTGCTTGCCG
  781 AATATCATGG TGGAAAATGG CCGCTTTTCT GGATTCATCG ACTGTGGCCG GCTGGGTGTG
  841 GCGGACCGCT ATCAGGACAT AGCGTTGGCT ACCCGTGATA TTGCTGAAGA GCTTGGCGGC
  901 GAATGGGCTG ACCGCTTCCT CGTGCTTTAC GGTATCGCCG CTCCCGATTC GCAGCGCATC
  961 GCCTTCTATC GCCTTCTTGA CGAGTTCTTC TGAGCGGGAC TCTGGGGTTC GAAATGACCG
 1021 ACCAAGCGAC GCCCAACCCT GCCATCACGA GATTTCGATT CCACCGCCGC CCTTTCTATG
 1081 AAAGGTTGGG CTTTCGGATC CGATTTTCCG GACGCTGCTG ATGAATTCCT TCAGCGGCGG
1141 GGATCTCAAT GGCATGTGGG GAGGTATCTT TCTCGCGCTC CAAC
//
Primer: pAxCALNL_F1
Sequence file: RDB04699_A4Kbb.seq check
>04699_04699_A4Kb_15_pAxCALNL_F1_A04_01.ab1
    1 ACAGGCTTCT TATCATGTCT GGATCAATCC GAACGCGTAT AACTTCGTAT AGCATACATT
   61 ATACGAAGTT ATCTCGAGTC GCTCGGTACG ATTTTCGAGC ACTGTTGGCC TACTGGGTAA
  121 TTCAGCGGTT TCCGGAGCTG CGGCGGCGCA GACTGGGAGG GGGAGCCGGG GGTTCCGACG
  181 TCGCAGCCGA GGGAACAAGC CCCAACCGGA TCCTGGACAG GCACCCCGGC TTGGCGCTGT
  241 CTCTCCCCCT CGGCTCGGAG AGGCCCTTCG GCCTGAGGGA GCCTCGCCGC CCGTCCCCGG
  301 CACACGCGCA GCCCCGGCCT CTCGGCCTCT GCCGGAGAAA CAGGATGGCC CAATGGAATC
  361 AGCTACAGCA GCTTGACACA CGGTACCTGG AGCAGCTCCA TCAGCTCTAC AGTGACAGCT
  421 TCCCAATGGA GCTGCGGCAG TTTCTGGCCC CTTGGATTGA GAGTCAAGAT TGGGCATATG
  481 CGGCCAGCAA AGAATCACAT GCCACTTTGG TGTTTCATAA TCTCCTGGGA GAGATTGACC
  541 AGCAGTATAG CCGCTTCCTG CAAGAGTCGA ATGTTCTCTA TCAGCACAAT CTACGAAGAA
  601 TCAAGCAGTT TCTTCAGAGC AGGTATCTTG AGAAGCCCAA TGGAGATTGC CCGGATTGTG
  661 GCCCGGTGCC TGTGGGAAGA ATCACGCCTT CTACAGACTG CAGCCACTGC GGGCCCCAGC
  721 AAGGGGGCCA GGCCAACCAC CCCACAGCAG CCGTGGTGAC GGAGAAGCAG CATATGCTGG
  781 AGCACCACCT TCGGGATGTC CGGAACAACA GTGCAGGATC TAGAACAGAA TATGAAAGTG
  841 AGTAGAGAAT CTTCCGGAAT GACCTTTGAT TTACAACTAT AAAAGCCTTC ATCGTGTCTA
901 ACGAGATATG GCGACATT
//
Primer: pAxCA_R1
Sequence file: RDB04699_A4Kbc check
1

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles