Resource data sheet
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pCMVhVEGF

Expression vector of human VEGF cDNA

Catalog number RDB03961
Resource name pCMVhVEGF
Clone info. Expression vector of human VEGF driven by CMV promoter.
Eypression was confirmed with ELISA by depositor.
Vector backbone pCMV (Plasmid)
Selectable markers Amp^r
Gene/insert name Human VEGF cDNA
Depositor Hamada, Hirofumi |
 
Other clones in our bank human VEGFA (NCBI Gene 7422) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.(J. Gene Med., 5, 900-908 (2003).) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB03961 pCMVhVEGF DNA solution Please contact us.

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Featured content

References

original , J. Gene Med., 5, 900-908 (2003). PMID 14533199.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB12357_A4Am.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: RDB12357_A4Ama.seq
>03961_12357_A4Am_CMV-Foward_E10_13.ab1
    1 CGTTCACTCC GCCCATTGAC GCAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA
   61 GCAGAGCTCG TTTAGTGAAC CGTCAGATCG CCTGGAGACG CCATCCACGC TGTTTTGACC
  121 TCCATAGAAG ACACCGGGAC CGATCCAGCC TCCGGACTCT AGAGGATCCG GTACTCGAGG
  181 AACTGAAAAA CCAGAAAGTT AACTGGTAAG TTTAGTCTTT TTGTCTTTTA TTTCAGGTCC
  241 CGGATCCGGT GGTGGTGCAA ATCAAAGAAC TGCTCCTCAG TGGATGTTGC CTTTACTTCT
  301 AGGCCTGTAC GGAAGTGTTA CTTCTGCTCT AAAAGCTGCG GAATTGTACC CGCGGCCAAT
  361 TCACCATGAA CTTTCTGCTG TCTTGGGTGC ATTGGAGCCT TGCCTTGCTG CTCTACCTCC
  421 ACCATGCCAA GTGGTCCCAG GCTGCACCCA TGGCAGAAGG AGGAGGGCAG AATCATCACG
  481 AAGTGGTGAA GTTCATGGAT GTCTATCAGC GCAGCTACTG CCATCCAATC GAGACCCTGG
  541 TGGACATCTT CCAGGAGTAC CCTGATGAGA TCGAGTACAT CTTCAAGCCA TCCTGTGTGC
  601 CCCTGATGCG ATGCGGGGGC TGCTGCAATG ACGAGGGCCT GGAGTGTGTG CCCACTGAGG
  661 AGTCCAACAT CACCATGCAG ATTATGCGGA TCAAACCTCA CCAAGGCCAG CACATAGGAG
  721 AGATGAGCTT CCTACAGCAC AACAAATGTG AATGCAGACC AAAGAAAGAT AGAGCAAGAC
  781 AAGAAAATCC CTGTGGGCCT TGCTCAGAGC GGAGAAAGCA TTTGTTTGTA CAAGATCCGC
  841 AGACGTGTAA ATGTTCCTGC AAAAACACAG ACTCGCGTTG CAAGGCGAGG CAGCTTGAGT
  901 TAAACGAACG TACTTGCAGA TGTGACAAGC CGAGGCGGTG AGGATCGGCC GCGGGGATCC
  961 AGACATGATA AGATACATTG ATGAGTTTGG ACAAACCACA ACTAGAATGC AGTGAAAAAA
 1021 AATGCTTTTA TTTGTGAAAT TTGTGATGCT ATTGCTTTTA TTTGTAACCA TTATAAGCTG
 1081 CAATAACAGT ACCACCACAT TGCATTCATT TTTATGTTTC CAGGTCCATG GAAGGTGTGG
1141 AAGATTTTTC GGAATCCTCA TAGGAGTTCG AACCCTGTGC CAAGGG
//
Primer: RDB12357_A4Amb.seq
>03961_12357_A4Am_pGCAP_R_F10_16.ab1
    1 ATATTCTTAA TCATGTCTGG ATCCCCGCGG CCGATCCTCA CCGCCTCGGC TTGTCACATC
   61 TGCAAGTACG TTCGTTTAAC TCAAGCTGCC TCGCCTTGCA ACGCGAGTCT GTGTTTTTGC
  121 AGGAACATTT ACACGTCTGC GGATCTTGTA CAAACAAATG CTTTCTCCGC TCTGAGCAAG
  181 GCCCACAGGG ATTTTCTTGT CTTGCTCTAT CTTTCTTTGG TCTGCATTCA CATTTGTTGT
  241 GCTGTAGGAA GCTCATCTCT CCTATGTGCT GGCCTTGGTG AGGTTTGATC CGCATAATCT
  301 GCATGGTGAT GTTGGACTCC TCAGTGGGCA CACACTCCAG GCCCTCGTCA TTGCAGCAGC
  361 CCCCGCATCG CATCAGGGGC ACACAGGATG GCTTGAAGAT GTACTCGATC TCATCAGGGT
  421 ACTCCTGGAA GATGTCCACC AGGGTCTCGA TTGGATGGCA GTAGCTGCGC TGATAGACAT
  481 CCATGAACTT CACCACTTCG TGATGATTCT GCCCTCCTCC TTCTGCCATG GGTGCAGCCT
  541 GGGACCACTT GGCATGGTGG AGGTAGAGCA GCAAGGCAAG GCTCCAATGC ACCCAAGACA
  601 GCAGAAAGTT CATGGTGAAT TGGCCGCGGG TACAATTCCG CAGCTTTTAG AGCAGAAGTA
  661 ACACTTCCGT ACAGGCCTAG AAGTAAAGGC AACATCCACT GAGGAGCAGT TCTTTGATTT
  721 GCACCACCAC CGGATCCGGG ACCTGAAATA AAAGACAAAA AGACTAAACT TACCAGTTAA
  781 CTTTCTGGTT TTTCAGTTCC TCGAGTACCG GATCCTCTAG AGTCCGGAGG CTGGATCGGT
  841 CCCGGTGTCT TCTATGGAGG TCAAAACAGC GTGGATGGCG TCTCCAGGCG ATCTGACGGT
  901 TCACTAAACG AGCTCTGCTT ATATAGACCT CCCACCGTAC ACGCCTACCG CCCATTTGCG
  961 TCAATGGGGC GGAGTTGTTA CGACATTTTG GAAAGTCCCG TTGATTTTGG TGCCAAAACA
 1021 AACTCCCATT GACGTCAATG GGGTGAGACT TGGAAAATCC CCGTGAGTCA ACCGCTATCC
 1081 ACGCCCATTT GATGTACTGC AAAACGCATC ACATGGTAAT AGCGATGACT TATACGTAGA
1141 TGTACTGGCT AGTTAGTAAG TTCCCATAAG GTCATGTGTT AACTTGGGGG CCC
//

Please visit Sequencing and PCR primers for primer information.


References

References

original , J. Gene Med., 5, 900-908 (2003). PMID 14533199.

2018.12.10

GNP_filter3_RDBDEP_html_181012.pl